Synthesis and characterization of fluorescent PVA/pvac-rodhamine microspheres

Autores
Ambrosio Tellez, Luisa Fernanda; Verón, María Gisela; Silin, Nicolas; Prado, Miguel Oscar
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fluorescent polymeric microspheres are used in a wide range of biomedical and technological applications, including blood flow determination, screening, flow visualization, flow tracing, medical imaging, fluorescence microscopy and flow cytometry among others. Rhodamine 6G (R6G) is a lipophilic cationic dye, which is among the most stable organic fluorophores, and with a high quantum yield in fluorescence (absorption and emission) in the visible range (500-600 nm). It is mainly applied in laser technology, and also in fluorescent bioimage. In this work we present an efficient synthesis route to obtain Polyvinyl Alcohol/Polyvinyl Acetate (PVA/PVAc) spherical microparticles containing Rhodamine 6G in a concentration sufficient to exploit their fluorescent properties. The synthesis method comprises the suspension polymerization of Vinyl acetate (VAc) monomer in the presence of Rhodamine, followed by saponification. Microspheres loaded with 1.9% w / w of Rhodamine 6G were obtained. They presented good sphericity and a size distribution in the range of 20 to 1550 microns. The fluorescence intensity of the microspheres was measured by UV-Vis and fluorescence spectroscopy, and detected using an image-based experimental setup, respectively. The results obtained with these methodologies showed that our microspheres exhibit fluorescence and that they are quite detectable in water flows. However, the response of UV-Visible fluorescence and absorbance was affected by the presence of the PVA / PVAc polymer matrix.
Fil: Ambrosio Tellez, Luisa Fernanda. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina
Fil: Verón, María Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina
Fil: Silin, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina
Fil: Prado, Miguel Oscar. Comisión Nacional de Energía Atómica. Gerencia del Área de Energía Nuclear. Instituto Balseiro; Argentina. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche; Argentina
Materia
FLUORESCENCE
MICROSPHERES
POLYVINYL ALCOHOL
RHODAMINE MOLECULE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/123887
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/123887
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Synthesis and characterization of fluorescent PVA/pvac-rodhamine microspheresAmbrosio Tellez, Luisa FernandaVerón, María GiselaSilin, NicolasPrado, Miguel OscarFLUORESCENCEMICROSPHERESPOLYVINYL ALCOHOLRHODAMINE MOLECULEhttps://purl.org/becyt/ford/2.5https://purl.org/becyt/ford/2Fluorescent polymeric microspheres are used in a wide range of biomedical and technological applications, including blood flow determination, screening, flow visualization, flow tracing, medical imaging, fluorescence microscopy and flow cytometry among others. Rhodamine 6G (R6G) is a lipophilic cationic dye, which is among the most stable organic fluorophores, and with a high quantum yield in fluorescence (absorption and emission) in the visible range (500-600 nm). It is mainly applied in laser technology, and also in fluorescent bioimage. In this work we present an efficient synthesis route to obtain Polyvinyl Alcohol/Polyvinyl Acetate (PVA/PVAc) spherical microparticles containing Rhodamine 6G in a concentration sufficient to exploit their fluorescent properties. The synthesis method comprises the suspension polymerization of Vinyl acetate (VAc) monomer in the presence of Rhodamine, followed by saponification. Microspheres loaded with 1.9% w / w of Rhodamine 6G were obtained. They presented good sphericity and a size distribution in the range of 20 to 1550 microns. The fluorescence intensity of the microspheres was measured by UV-Vis and fluorescence spectroscopy, and detected using an image-based experimental setup, respectively. The results obtained with these methodologies showed that our microspheres exhibit fluorescence and that they are quite detectable in water flows. However, the response of UV-Visible fluorescence and absorbance was affected by the presence of the PVA / PVAc polymer matrix.Fil: Ambrosio Tellez, Luisa Fernanda. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; ArgentinaFil: Verón, María Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; ArgentinaFil: Silin, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; ArgentinaFil: Prado, Miguel Oscar. Comisión Nacional de Energía Atómica. Gerencia del Área de Energía Nuclear. Instituto Balseiro; Argentina. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche; ArgentinaUniversidade Federal de São Carlos2019-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/123887Ambrosio Tellez, Luisa Fernanda; Verón, María Gisela; Silin, Nicolas; Prado, Miguel Oscar; Synthesis and characterization of fluorescent PVA/pvac-rodhamine microspheres; Universidade Federal de São Carlos; Materials Research; 22; 4; 7-2019; 1-91516-1439CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392019000400225&tlng=eninfo:eu-repo/semantics/altIdentifier/doi/10.1590/1980-5373-mr-2019-0133info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:35Zoai:ri.conicet.gov.ar:11336/123887instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:36.1CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Synthesis and characterization of fluorescent PVA/pvac-rodhamine microspheres
title Synthesis and characterization of fluorescent PVA/pvac-rodhamine microspheres
spellingShingle Synthesis and characterization of fluorescent PVA/pvac-rodhamine microspheres
Ambrosio Tellez, Luisa Fernanda
FLUORESCENCE
MICROSPHERES
POLYVINYL ALCOHOL
RHODAMINE MOLECULE
title_short Synthesis and characterization of fluorescent PVA/pvac-rodhamine microspheres
title_full Synthesis and characterization of fluorescent PVA/pvac-rodhamine microspheres
title_fullStr Synthesis and characterization of fluorescent PVA/pvac-rodhamine microspheres
title_full_unstemmed Synthesis and characterization of fluorescent PVA/pvac-rodhamine microspheres
title_sort Synthesis and characterization of fluorescent PVA/pvac-rodhamine microspheres
dc.creator.none.fl_str_mv Ambrosio Tellez, Luisa Fernanda
Verón, María Gisela
Silin, Nicolas
Prado, Miguel Oscar
author Ambrosio Tellez, Luisa Fernanda
author_facet Ambrosio Tellez, Luisa Fernanda
Verón, María Gisela
Silin, Nicolas
Prado, Miguel Oscar
author_role author
author2 Verón, María Gisela
Silin, Nicolas
Prado, Miguel Oscar
author2_role author
author
author
dc.subject.none.fl_str_mv FLUORESCENCE
MICROSPHERES
POLYVINYL ALCOHOL
RHODAMINE MOLECULE
topic FLUORESCENCE
MICROSPHERES
POLYVINYL ALCOHOL
RHODAMINE MOLECULE
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.5
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Fluorescent polymeric microspheres are used in a wide range of biomedical and technological applications, including blood flow determination, screening, flow visualization, flow tracing, medical imaging, fluorescence microscopy and flow cytometry among others. Rhodamine 6G (R6G) is a lipophilic cationic dye, which is among the most stable organic fluorophores, and with a high quantum yield in fluorescence (absorption and emission) in the visible range (500-600 nm). It is mainly applied in laser technology, and also in fluorescent bioimage. In this work we present an efficient synthesis route to obtain Polyvinyl Alcohol/Polyvinyl Acetate (PVA/PVAc) spherical microparticles containing Rhodamine 6G in a concentration sufficient to exploit their fluorescent properties. The synthesis method comprises the suspension polymerization of Vinyl acetate (VAc) monomer in the presence of Rhodamine, followed by saponification. Microspheres loaded with 1.9% w / w of Rhodamine 6G were obtained. They presented good sphericity and a size distribution in the range of 20 to 1550 microns. The fluorescence intensity of the microspheres was measured by UV-Vis and fluorescence spectroscopy, and detected using an image-based experimental setup, respectively. The results obtained with these methodologies showed that our microspheres exhibit fluorescence and that they are quite detectable in water flows. However, the response of UV-Visible fluorescence and absorbance was affected by the presence of the PVA / PVAc polymer matrix.
Fil: Ambrosio Tellez, Luisa Fernanda. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina
Fil: Verón, María Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina
Fil: Silin, Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina
Fil: Prado, Miguel Oscar. Comisión Nacional de Energía Atómica. Gerencia del Área de Energía Nuclear. Instituto Balseiro; Argentina. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche; Argentina
description Fluorescent polymeric microspheres are used in a wide range of biomedical and technological applications, including blood flow determination, screening, flow visualization, flow tracing, medical imaging, fluorescence microscopy and flow cytometry among others. Rhodamine 6G (R6G) is a lipophilic cationic dye, which is among the most stable organic fluorophores, and with a high quantum yield in fluorescence (absorption and emission) in the visible range (500-600 nm). It is mainly applied in laser technology, and also in fluorescent bioimage. In this work we present an efficient synthesis route to obtain Polyvinyl Alcohol/Polyvinyl Acetate (PVA/PVAc) spherical microparticles containing Rhodamine 6G in a concentration sufficient to exploit their fluorescent properties. The synthesis method comprises the suspension polymerization of Vinyl acetate (VAc) monomer in the presence of Rhodamine, followed by saponification. Microspheres loaded with 1.9% w / w of Rhodamine 6G were obtained. They presented good sphericity and a size distribution in the range of 20 to 1550 microns. The fluorescence intensity of the microspheres was measured by UV-Vis and fluorescence spectroscopy, and detected using an image-based experimental setup, respectively. The results obtained with these methodologies showed that our microspheres exhibit fluorescence and that they are quite detectable in water flows. However, the response of UV-Visible fluorescence and absorbance was affected by the presence of the PVA / PVAc polymer matrix.
publishDate 2019
dc.date.none.fl_str_mv 2019-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/123887
Ambrosio Tellez, Luisa Fernanda; Verón, María Gisela; Silin, Nicolas; Prado, Miguel Oscar; Synthesis and characterization of fluorescent PVA/pvac-rodhamine microspheres; Universidade Federal de São Carlos; Materials Research; 22; 4; 7-2019; 1-9
1516-1439
CONICET Digital
CONICET
url http://hdl.handle.net/11336/123887
identifier_str_mv Ambrosio Tellez, Luisa Fernanda; Verón, María Gisela; Silin, Nicolas; Prado, Miguel Oscar; Synthesis and characterization of fluorescent PVA/pvac-rodhamine microspheres; Universidade Federal de São Carlos; Materials Research; 22; 4; 7-2019; 1-9
1516-1439
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392019000400225&tlng=en
info:eu-repo/semantics/altIdentifier/doi/10.1590/1980-5373-mr-2019-0133
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de São Carlos
publisher.none.fl_str_mv Universidade Federal de São Carlos
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268677429264384
score 13.13397

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