Detection of class 1 and 2 integrons, extended-spectrum β-lactamases and qnr alleles in enterobacterial isolates from the digestive tract of Intensive Care Unit inpatients

Autores
Bado, Inés; Cordeiro, Nicolás F.; Robino, Luciana; García Fulgueiras, Virginia; Seija, Verónica; Bazet, Cristina; Gutkind, Gabriel Osvaldo; Ayala, Juan A.; Vignoli, Rafael
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this study, we searched for extended-spectrum β-lactamases (ESBLs), class 1 and 2 integrons, and qnrA, qnrB and qnrS genes in 56 oxyimino-cephalosporin and/or ciprofloxacin-resistant enterobacterial isolates obtained from the gastrointestinal tract of patients admitted in an Intensive Care Unit in Uruguay. ESBLs were detected in 11 isolates (6 CTX-M-2, 3 CTX-M-9, 1 CTX-M-15 and 1 PER-2). qnr genes and integrons were detected in 5 and 24 isolates, respectively. Eight different antibiotic resistance gene cassettes were found within six different genetic arrangements. Two types of complex class 1 integrons carrying insertion sequence ISCR1 were found, one showing blaCTX-M-2–orf3 and the other qnrA1–ampR. Ten of the thirteen isolates carrying class 2 integrons presented the element IS5 inserted between intI2 and dfrA1, whereas another class 2 integron lacked the internal stop codon usually present in intI2. This is the first report of the occurrence of qnrA, qnrB and blaCTX-M-9 in Uruguay. Dissemination of the different groups of CTX-M enzymes (i.e. groups 1, 2 and 9) appears to be a recent phenomenon in South America.
Fil: Bado, Inés. Universidad de la Republica; Uruguay
Fil: Cordeiro, Nicolás F.. Universidad de la Republica; Uruguay
Fil: Robino, Luciana. Universidad de la Republica; Uruguay
Fil: García Fulgueiras, Virginia. Universidad de la Republica; Uruguay
Fil: Seija, Verónica. Universidad de la Republica; Uruguay
Fil: Bazet, Cristina. Universidad de la Republica; Uruguay
Fil: Gutkind, Gabriel Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Ayala, Juan A.. Consejo Superior de Investigaciones Cientificas; España. Universidad Autónoma de Madrid; España
Fil: Vignoli, Rafael. Universidad de la Republica; Uruguay
Materia
Integrons
Transferable
Resistance
Blactx-M
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14245
Acceder
id CONICETDig_59e00f88fc08b456fd938ac8c9b70541
oai_identifier_str oai:ri.conicet.gov.ar:11336/14245
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Detection of class 1 and 2 integrons, extended-spectrum β-lactamases and qnr alleles in enterobacterial isolates from the digestive tract of Intensive Care Unit inpatientsBado, InésCordeiro, Nicolás F.Robino, LucianaGarcía Fulgueiras, VirginiaSeija, VerónicaBazet, CristinaGutkind, Gabriel OsvaldoAyala, Juan A.Vignoli, RafaelIntegronsTransferableResistanceBlactx-Mhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3In this study, we searched for extended-spectrum β-lactamases (ESBLs), class 1 and 2 integrons, and qnrA, qnrB and qnrS genes in 56 oxyimino-cephalosporin and/or ciprofloxacin-resistant enterobacterial isolates obtained from the gastrointestinal tract of patients admitted in an Intensive Care Unit in Uruguay. ESBLs were detected in 11 isolates (6 CTX-M-2, 3 CTX-M-9, 1 CTX-M-15 and 1 PER-2). qnr genes and integrons were detected in 5 and 24 isolates, respectively. Eight different antibiotic resistance gene cassettes were found within six different genetic arrangements. Two types of complex class 1 integrons carrying insertion sequence ISCR1 were found, one showing blaCTX-M-2–orf3 and the other qnrA1–ampR. Ten of the thirteen isolates carrying class 2 integrons presented the element IS5 inserted between intI2 and dfrA1, whereas another class 2 integron lacked the internal stop codon usually present in intI2. This is the first report of the occurrence of qnrA, qnrB and blaCTX-M-9 in Uruguay. Dissemination of the different groups of CTX-M enzymes (i.e. groups 1, 2 and 9) appears to be a recent phenomenon in South America.Fil: Bado, Inés. Universidad de la Republica; UruguayFil: Cordeiro, Nicolás F.. Universidad de la Republica; UruguayFil: Robino, Luciana. Universidad de la Republica; UruguayFil: García Fulgueiras, Virginia. Universidad de la Republica; UruguayFil: Seija, Verónica. Universidad de la Republica; UruguayFil: Bazet, Cristina. Universidad de la Republica; UruguayFil: Gutkind, Gabriel Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Ayala, Juan A.. Consejo Superior de Investigaciones Cientificas; España. Universidad Autónoma de Madrid; EspañaFil: Vignoli, Rafael. Universidad de la Republica; UruguayElsevier Science2010-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14245Bado, Inés; Cordeiro, Nicolás F.; Robino, Luciana; García Fulgueiras, Virginia; Seija, Verónica; et al.; Detection of class 1 and 2 integrons, extended-spectrum β-lactamases and qnr alleles in enterobacterial isolates from the digestive tract of Intensive Care Unit inpatients; Elsevier Science; International Journal Of Antimicrobial Agents; 36; 5; 11-2010; 453-4580924-8579enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0924857910003006info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijantimicag.2010.06.042info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:33Zoai:ri.conicet.gov.ar:11336/14245instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:33.714CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Detection of class 1 and 2 integrons, extended-spectrum β-lactamases and qnr alleles in enterobacterial isolates from the digestive tract of Intensive Care Unit inpatients
title Detection of class 1 and 2 integrons, extended-spectrum β-lactamases and qnr alleles in enterobacterial isolates from the digestive tract of Intensive Care Unit inpatients
spellingShingle Detection of class 1 and 2 integrons, extended-spectrum β-lactamases and qnr alleles in enterobacterial isolates from the digestive tract of Intensive Care Unit inpatients
Bado, Inés
Integrons
Transferable
Resistance
Blactx-M
title_short Detection of class 1 and 2 integrons, extended-spectrum β-lactamases and qnr alleles in enterobacterial isolates from the digestive tract of Intensive Care Unit inpatients
title_full Detection of class 1 and 2 integrons, extended-spectrum β-lactamases and qnr alleles in enterobacterial isolates from the digestive tract of Intensive Care Unit inpatients
title_fullStr Detection of class 1 and 2 integrons, extended-spectrum β-lactamases and qnr alleles in enterobacterial isolates from the digestive tract of Intensive Care Unit inpatients
title_full_unstemmed Detection of class 1 and 2 integrons, extended-spectrum β-lactamases and qnr alleles in enterobacterial isolates from the digestive tract of Intensive Care Unit inpatients
title_sort Detection of class 1 and 2 integrons, extended-spectrum β-lactamases and qnr alleles in enterobacterial isolates from the digestive tract of Intensive Care Unit inpatients
dc.creator.none.fl_str_mv Bado, Inés
Cordeiro, Nicolás F.
Robino, Luciana
García Fulgueiras, Virginia
Seija, Verónica
Bazet, Cristina
Gutkind, Gabriel Osvaldo
Ayala, Juan A.
Vignoli, Rafael
author Bado, Inés
author_facet Bado, Inés
Cordeiro, Nicolás F.
Robino, Luciana
García Fulgueiras, Virginia
Seija, Verónica
Bazet, Cristina
Gutkind, Gabriel Osvaldo
Ayala, Juan A.
Vignoli, Rafael
author_role author
author2 Cordeiro, Nicolás F.
Robino, Luciana
García Fulgueiras, Virginia
Seija, Verónica
Bazet, Cristina
Gutkind, Gabriel Osvaldo
Ayala, Juan A.
Vignoli, Rafael
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Integrons
Transferable
Resistance
Blactx-M
topic Integrons
Transferable
Resistance
Blactx-M
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In this study, we searched for extended-spectrum β-lactamases (ESBLs), class 1 and 2 integrons, and qnrA, qnrB and qnrS genes in 56 oxyimino-cephalosporin and/or ciprofloxacin-resistant enterobacterial isolates obtained from the gastrointestinal tract of patients admitted in an Intensive Care Unit in Uruguay. ESBLs were detected in 11 isolates (6 CTX-M-2, 3 CTX-M-9, 1 CTX-M-15 and 1 PER-2). qnr genes and integrons were detected in 5 and 24 isolates, respectively. Eight different antibiotic resistance gene cassettes were found within six different genetic arrangements. Two types of complex class 1 integrons carrying insertion sequence ISCR1 were found, one showing blaCTX-M-2–orf3 and the other qnrA1–ampR. Ten of the thirteen isolates carrying class 2 integrons presented the element IS5 inserted between intI2 and dfrA1, whereas another class 2 integron lacked the internal stop codon usually present in intI2. This is the first report of the occurrence of qnrA, qnrB and blaCTX-M-9 in Uruguay. Dissemination of the different groups of CTX-M enzymes (i.e. groups 1, 2 and 9) appears to be a recent phenomenon in South America.
Fil: Bado, Inés. Universidad de la Republica; Uruguay
Fil: Cordeiro, Nicolás F.. Universidad de la Republica; Uruguay
Fil: Robino, Luciana. Universidad de la Republica; Uruguay
Fil: García Fulgueiras, Virginia. Universidad de la Republica; Uruguay
Fil: Seija, Verónica. Universidad de la Republica; Uruguay
Fil: Bazet, Cristina. Universidad de la Republica; Uruguay
Fil: Gutkind, Gabriel Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Ayala, Juan A.. Consejo Superior de Investigaciones Cientificas; España. Universidad Autónoma de Madrid; España
Fil: Vignoli, Rafael. Universidad de la Republica; Uruguay
description In this study, we searched for extended-spectrum β-lactamases (ESBLs), class 1 and 2 integrons, and qnrA, qnrB and qnrS genes in 56 oxyimino-cephalosporin and/or ciprofloxacin-resistant enterobacterial isolates obtained from the gastrointestinal tract of patients admitted in an Intensive Care Unit in Uruguay. ESBLs were detected in 11 isolates (6 CTX-M-2, 3 CTX-M-9, 1 CTX-M-15 and 1 PER-2). qnr genes and integrons were detected in 5 and 24 isolates, respectively. Eight different antibiotic resistance gene cassettes were found within six different genetic arrangements. Two types of complex class 1 integrons carrying insertion sequence ISCR1 were found, one showing blaCTX-M-2–orf3 and the other qnrA1–ampR. Ten of the thirteen isolates carrying class 2 integrons presented the element IS5 inserted between intI2 and dfrA1, whereas another class 2 integron lacked the internal stop codon usually present in intI2. This is the first report of the occurrence of qnrA, qnrB and blaCTX-M-9 in Uruguay. Dissemination of the different groups of CTX-M enzymes (i.e. groups 1, 2 and 9) appears to be a recent phenomenon in South America.
publishDate 2010
dc.date.none.fl_str_mv 2010-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14245
Bado, Inés; Cordeiro, Nicolás F.; Robino, Luciana; García Fulgueiras, Virginia; Seija, Verónica; et al.; Detection of class 1 and 2 integrons, extended-spectrum β-lactamases and qnr alleles in enterobacterial isolates from the digestive tract of Intensive Care Unit inpatients; Elsevier Science; International Journal Of Antimicrobial Agents; 36; 5; 11-2010; 453-458
0924-8579
url http://hdl.handle.net/11336/14245
identifier_str_mv Bado, Inés; Cordeiro, Nicolás F.; Robino, Luciana; García Fulgueiras, Virginia; Seija, Verónica; et al.; Detection of class 1 and 2 integrons, extended-spectrum β-lactamases and qnr alleles in enterobacterial isolates from the digestive tract of Intensive Care Unit inpatients; Elsevier Science; International Journal Of Antimicrobial Agents; 36; 5; 11-2010; 453-458
0924-8579
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0924857910003006
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijantimicag.2010.06.042
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269291996512256
score 13.13397

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