Detection of integrons class 1 and class 2 in VTEC strains isolated from pigs

Autores
Colello, Rocío; Moredo, Fabiana Alicia; Etcheverría, Analía Inés; Leotta, Gerardo Anibal; Parma, Alberto Ernesto; Padola, Nora Lía
Año de publicación
2012
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Introduction and objectives: In the last 10 years, approximately 500 HUS new annual cases w ere reported in Argentina, w ith an incidence of 17/100,000 children under five years old. The production of VT1, VT2 and/or their variants is the primary virulence trait responsible for human disease. VT2e is typically associated w ith pig edema disease and has been detected only rarely in VTEC of human origin. Some studies have show n that VTEC serotypes have developed resistance to antibiotics used in human and veterinary medicine. Humans may obtain antibiotic-resistant E. coli by contact with animals, foods, or environment. The w idespread use of antibiotics creates a reservoir of resistant bacteria carrying antibiotic resistance genes. Integrons contain the genetic determinants of the components of a site-specific recombination system w hich recognize and capture the mobile antibiotic-resistance genes cassette. Integrons include a gene for an integrase (int), an adjacent recombination site (attl), and a strong promoter/s that ensure expression of the integrated cassettes.The aim of this study w as to detect integrons in VTEC strains with antibiotic multiresistance. Material & Methods: Tw enty-one VTEC strains isolated from pig faeces f rom ten farms (named A to J) f rom Argentina w ere analysed to detect integrons (int1 and int2) by PCR. Results: Out of tw enty-one analysed strains, 8 (38 %) carried integrons enconding genes. These strains belonged to 4 different farms. Conclusions: The use of antibiotics in animal production systems has determined that bacteria could develop resistance mechanisms originating strains w ith risk for human if entering a food chain. Integrons are not only associated w ith resistance to antibiotics, but also w ith the horizontal transference of resistance genes. Some studies have informed of the presence of integrons in Enterobacteriaceae and Escherichia coli in samples isolated f rom pigs, not being registered data in VTEC strains so far. In this study integrons class 1 and class 2 w ere detected in VTEC strains isolated from pigs show ing that inadequate use of antibiotic as therapeutic agents or grow ing promoter in veterinary, implies a risk for public health because the acquisition and the horizontal transference of integrons among strains.
Fil: Colello, Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Moredo, Fabiana Alicia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Etcheverría, Analía Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Leotta, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Parma, Alberto Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Padola, Nora Lía. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina
8th International Symposium on Shiga Toxin (Verocytotoxin) Producing Escherichia coli Infections
Amsterdam
Países Bajos
Radboud University Medical Center
Materia
VTEC
PIGS
INTEGRONS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/234018
Acceder
id CONICETDig_144c9c6aed7682e0f3332be1f95bd13f
oai_identifier_str oai:ri.conicet.gov.ar:11336/234018
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Detection of integrons class 1 and class 2 in VTEC strains isolated from pigsColello, RocíoMoredo, Fabiana AliciaEtcheverría, Analía InésLeotta, Gerardo AnibalParma, Alberto ErnestoPadola, Nora LíaVTECPIGSINTEGRONShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Introduction and objectives: In the last 10 years, approximately 500 HUS new annual cases w ere reported in Argentina, w ith an incidence of 17/100,000 children under five years old. The production of VT1, VT2 and/or their variants is the primary virulence trait responsible for human disease. VT2e is typically associated w ith pig edema disease and has been detected only rarely in VTEC of human origin. Some studies have show n that VTEC serotypes have developed resistance to antibiotics used in human and veterinary medicine. Humans may obtain antibiotic-resistant E. coli by contact with animals, foods, or environment. The w idespread use of antibiotics creates a reservoir of resistant bacteria carrying antibiotic resistance genes. Integrons contain the genetic determinants of the components of a site-specific recombination system w hich recognize and capture the mobile antibiotic-resistance genes cassette. Integrons include a gene for an integrase (int), an adjacent recombination site (attl), and a strong promoter/s that ensure expression of the integrated cassettes.The aim of this study w as to detect integrons in VTEC strains with antibiotic multiresistance. Material & Methods: Tw enty-one VTEC strains isolated from pig faeces f rom ten farms (named A to J) f rom Argentina w ere analysed to detect integrons (int1 and int2) by PCR. Results: Out of tw enty-one analysed strains, 8 (38 %) carried integrons enconding genes. These strains belonged to 4 different farms. Conclusions: The use of antibiotics in animal production systems has determined that bacteria could develop resistance mechanisms originating strains w ith risk for human if entering a food chain. Integrons are not only associated w ith resistance to antibiotics, but also w ith the horizontal transference of resistance genes. Some studies have informed of the presence of integrons in Enterobacteriaceae and Escherichia coli in samples isolated f rom pigs, not being registered data in VTEC strains so far. In this study integrons class 1 and class 2 w ere detected in VTEC strains isolated from pigs show ing that inadequate use of antibiotic as therapeutic agents or grow ing promoter in veterinary, implies a risk for public health because the acquisition and the horizontal transference of integrons among strains.Fil: Colello, Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Moredo, Fabiana Alicia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Etcheverría, Analía Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Leotta, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Parma, Alberto Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Padola, Nora Lía. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina8th International Symposium on Shiga Toxin (Verocytotoxin) Producing Escherichia coli InfectionsAmsterdamPaíses BajosRadboud University Medical CenterWiley2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectSimposioJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/234018Detection of integrons class 1 and class 2 in VTEC strains isolated from pigs; 8th International Symposium on Shiga Toxin (Verocytotoxin) Producing Escherichia coli Infections; Amsterdam; Países Bajos; 2012; 51-511863-2378CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/j.1863-2378.2012.01512.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1863-2378.2012.01512.xInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:26Zoai:ri.conicet.gov.ar:11336/234018instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:26.861CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Detection of integrons class 1 and class 2 in VTEC strains isolated from pigs
title Detection of integrons class 1 and class 2 in VTEC strains isolated from pigs
spellingShingle Detection of integrons class 1 and class 2 in VTEC strains isolated from pigs
Colello, Rocío
VTEC
PIGS
INTEGRONS
title_short Detection of integrons class 1 and class 2 in VTEC strains isolated from pigs
title_full Detection of integrons class 1 and class 2 in VTEC strains isolated from pigs
title_fullStr Detection of integrons class 1 and class 2 in VTEC strains isolated from pigs
title_full_unstemmed Detection of integrons class 1 and class 2 in VTEC strains isolated from pigs
title_sort Detection of integrons class 1 and class 2 in VTEC strains isolated from pigs
dc.creator.none.fl_str_mv Colello, Rocío
Moredo, Fabiana Alicia
Etcheverría, Analía Inés
Leotta, Gerardo Anibal
Parma, Alberto Ernesto
Padola, Nora Lía
author Colello, Rocío
author_facet Colello, Rocío
Moredo, Fabiana Alicia
Etcheverría, Analía Inés
Leotta, Gerardo Anibal
Parma, Alberto Ernesto
Padola, Nora Lía
author_role author
author2 Moredo, Fabiana Alicia
Etcheverría, Analía Inés
Leotta, Gerardo Anibal
Parma, Alberto Ernesto
Padola, Nora Lía
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv VTEC
PIGS
INTEGRONS
topic VTEC
PIGS
INTEGRONS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Introduction and objectives: In the last 10 years, approximately 500 HUS new annual cases w ere reported in Argentina, w ith an incidence of 17/100,000 children under five years old. The production of VT1, VT2 and/or their variants is the primary virulence trait responsible for human disease. VT2e is typically associated w ith pig edema disease and has been detected only rarely in VTEC of human origin. Some studies have show n that VTEC serotypes have developed resistance to antibiotics used in human and veterinary medicine. Humans may obtain antibiotic-resistant E. coli by contact with animals, foods, or environment. The w idespread use of antibiotics creates a reservoir of resistant bacteria carrying antibiotic resistance genes. Integrons contain the genetic determinants of the components of a site-specific recombination system w hich recognize and capture the mobile antibiotic-resistance genes cassette. Integrons include a gene for an integrase (int), an adjacent recombination site (attl), and a strong promoter/s that ensure expression of the integrated cassettes.The aim of this study w as to detect integrons in VTEC strains with antibiotic multiresistance. Material & Methods: Tw enty-one VTEC strains isolated from pig faeces f rom ten farms (named A to J) f rom Argentina w ere analysed to detect integrons (int1 and int2) by PCR. Results: Out of tw enty-one analysed strains, 8 (38 %) carried integrons enconding genes. These strains belonged to 4 different farms. Conclusions: The use of antibiotics in animal production systems has determined that bacteria could develop resistance mechanisms originating strains w ith risk for human if entering a food chain. Integrons are not only associated w ith resistance to antibiotics, but also w ith the horizontal transference of resistance genes. Some studies have informed of the presence of integrons in Enterobacteriaceae and Escherichia coli in samples isolated f rom pigs, not being registered data in VTEC strains so far. In this study integrons class 1 and class 2 w ere detected in VTEC strains isolated from pigs show ing that inadequate use of antibiotic as therapeutic agents or grow ing promoter in veterinary, implies a risk for public health because the acquisition and the horizontal transference of integrons among strains.
Fil: Colello, Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Moredo, Fabiana Alicia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Etcheverría, Analía Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Leotta, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Parma, Alberto Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Padola, Nora Lía. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquímica y Biotecnología; Argentina
8th International Symposium on Shiga Toxin (Verocytotoxin) Producing Escherichia coli Infections
Amsterdam
Países Bajos
Radboud University Medical Center
description Introduction and objectives: In the last 10 years, approximately 500 HUS new annual cases w ere reported in Argentina, w ith an incidence of 17/100,000 children under five years old. The production of VT1, VT2 and/or their variants is the primary virulence trait responsible for human disease. VT2e is typically associated w ith pig edema disease and has been detected only rarely in VTEC of human origin. Some studies have show n that VTEC serotypes have developed resistance to antibiotics used in human and veterinary medicine. Humans may obtain antibiotic-resistant E. coli by contact with animals, foods, or environment. The w idespread use of antibiotics creates a reservoir of resistant bacteria carrying antibiotic resistance genes. Integrons contain the genetic determinants of the components of a site-specific recombination system w hich recognize and capture the mobile antibiotic-resistance genes cassette. Integrons include a gene for an integrase (int), an adjacent recombination site (attl), and a strong promoter/s that ensure expression of the integrated cassettes.The aim of this study w as to detect integrons in VTEC strains with antibiotic multiresistance. Material & Methods: Tw enty-one VTEC strains isolated from pig faeces f rom ten farms (named A to J) f rom Argentina w ere analysed to detect integrons (int1 and int2) by PCR. Results: Out of tw enty-one analysed strains, 8 (38 %) carried integrons enconding genes. These strains belonged to 4 different farms. Conclusions: The use of antibiotics in animal production systems has determined that bacteria could develop resistance mechanisms originating strains w ith risk for human if entering a food chain. Integrons are not only associated w ith resistance to antibiotics, but also w ith the horizontal transference of resistance genes. Some studies have informed of the presence of integrons in Enterobacteriaceae and Escherichia coli in samples isolated f rom pigs, not being registered data in VTEC strains so far. In this study integrons class 1 and class 2 w ere detected in VTEC strains isolated from pigs show ing that inadequate use of antibiotic as therapeutic agents or grow ing promoter in veterinary, implies a risk for public health because the acquisition and the horizontal transference of integrons among strains.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Simposio
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/234018
Detection of integrons class 1 and class 2 in VTEC strains isolated from pigs; 8th International Symposium on Shiga Toxin (Verocytotoxin) Producing Escherichia coli Infections; Amsterdam; Países Bajos; 2012; 51-51
1863-2378
CONICET Digital
CONICET
url http://hdl.handle.net/11336/234018
identifier_str_mv Detection of integrons class 1 and class 2 in VTEC strains isolated from pigs; 8th International Symposium on Shiga Toxin (Verocytotoxin) Producing Escherichia coli Infections; Amsterdam; Países Bajos; 2012; 51-51
1863-2378
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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