Extended-spectrum β-lactamases and plasmid-mediated quinolone resistance in enterobacterial clinical isolates in the paediatric hospital of Uruguay

Autores
García Fulgueiras, Virginia; Bado, Inés; Mota, María Inés; Robino, Luciana; Cordeiro, Nicolás F.; Varela, Adriana; Algorta, Gabriela; Gutkind, Gabriel Osvaldo; Ayala, Juan A.; Vignoli, Rafael
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objectives To analyse the prevalence of resistance to β-lactams and plasmid-mediated quinolone resistance in Enterobacteriaceae in the paediatric hospital of Uruguay. Methods A total of 368 enterobacterial isolates collected between 1 May and 30 November 2009 were studied for the presence of extended-spectrum β-lactamases (ESBLs), qnr alleles and aac(6′)Ib by phenotypic and molecular methods. The genomic context and transferability of β-lactamase and qnr genes were examined by PCR and conjugation, respectively. Results The proportion of inpatients having an infection caused by ESBL-producing enterobacteria was 0.23% (16/7073) in paediatrics wards, 0.64‰ (3/4696) in the neonatology department and 0.03‰ (1/32 557) in the emergency department. ESBL-carrying enterobacteria constituted a total of 21.6% (16/74), 13% (3/23) and 0.37% (1/271) when samples were obtained from paediatrics wards, the neonatology department and the emergency department, respectively. Overall, CTX-M-2 (n = 7), CTX-M-9 (n = 3), CTX-M-8 (n = 2), CTX-M-15 (n = 1), SHV-5 (n = 5) and SHV-2 (n = 2) β-lactamases were detected. Thirteen out of 20 ESBL-producing isolates also carried the aac(6′)Ib gene, and the cr variant was detected in one of them. qnr alleles were detected in four isolates comprising two qnrA1 genes, a qnrB8-like variant and a new qnrB gene showing 26 amino acid differences from QnrB1. Conclusions The proportion of ESBL-producing enterobacteria in Uruguay's paediatric hospital during the study period was 2.3 per 1000 hospitalized patients. The number of different microorganisms detected, as well as the various EBSLs, suggests the occurrence of sporadic episodes instead of nosocomial outbreaks. Nevertheless, the presence of new resistance genes reinforces the necessity for permanent surveillance programmes.
Fil: García Fulgueiras, Virginia. Universidad de la Republica; Uruguay
Fil: Bado, Inés. Universidad de la Republica; Uruguay
Fil: Mota, María Inés. Universidad de la Republica; Uruguay. Uruguay. Ministerio de Salud Pública; Uruguay
Fil: Robino, Luciana. Universidad de la Republica; Uruguay
Fil: Cordeiro, Nicolás F.. Universidad de la Republica; Uruguay
Fil: Varela, Adriana. Uruguay. Ministerio de Salud Pública; Uruguay
Fil: Algorta, Gabriela. Universidad de la Republica; Uruguay. Uruguay. Ministerio de Salud Pública; Uruguay
Fil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Ayala, Juan A.. Universidad Autónoma de Madrid; España. Consejo Superior de Investigaciones Cientificas; España
Fil: Vignoli, Rafael. Universidad de la Republica; Uruguay
Materia
Antibiotic Resistance
Enterobacteriaceae
Integrons
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/12923
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/12923
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Extended-spectrum β-lactamases and plasmid-mediated quinolone resistance in enterobacterial clinical isolates in the paediatric hospital of UruguayGarcía Fulgueiras, VirginiaBado, InésMota, María InésRobino, LucianaCordeiro, Nicolás F.Varela, AdrianaAlgorta, GabrielaGutkind, Gabriel OsvaldoAyala, Juan A.Vignoli, RafaelAntibiotic ResistanceEnterobacteriaceaeIntegronshttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Objectives To analyse the prevalence of resistance to β-lactams and plasmid-mediated quinolone resistance in Enterobacteriaceae in the paediatric hospital of Uruguay. Methods A total of 368 enterobacterial isolates collected between 1 May and 30 November 2009 were studied for the presence of extended-spectrum β-lactamases (ESBLs), qnr alleles and aac(6′)Ib by phenotypic and molecular methods. The genomic context and transferability of β-lactamase and qnr genes were examined by PCR and conjugation, respectively. Results The proportion of inpatients having an infection caused by ESBL-producing enterobacteria was 0.23% (16/7073) in paediatrics wards, 0.64‰ (3/4696) in the neonatology department and 0.03‰ (1/32 557) in the emergency department. ESBL-carrying enterobacteria constituted a total of 21.6% (16/74), 13% (3/23) and 0.37% (1/271) when samples were obtained from paediatrics wards, the neonatology department and the emergency department, respectively. Overall, CTX-M-2 (n = 7), CTX-M-9 (n = 3), CTX-M-8 (n = 2), CTX-M-15 (n = 1), SHV-5 (n = 5) and SHV-2 (n = 2) β-lactamases were detected. Thirteen out of 20 ESBL-producing isolates also carried the aac(6′)Ib gene, and the cr variant was detected in one of them. qnr alleles were detected in four isolates comprising two qnrA1 genes, a qnrB8-like variant and a new qnrB gene showing 26 amino acid differences from QnrB1. Conclusions The proportion of ESBL-producing enterobacteria in Uruguay's paediatric hospital during the study period was 2.3 per 1000 hospitalized patients. The number of different microorganisms detected, as well as the various EBSLs, suggests the occurrence of sporadic episodes instead of nosocomial outbreaks. Nevertheless, the presence of new resistance genes reinforces the necessity for permanent surveillance programmes.Fil: García Fulgueiras, Virginia. Universidad de la Republica; UruguayFil: Bado, Inés. Universidad de la Republica; UruguayFil: Mota, María Inés. Universidad de la Republica; Uruguay. Uruguay. Ministerio de Salud Pública; UruguayFil: Robino, Luciana. Universidad de la Republica; UruguayFil: Cordeiro, Nicolás F.. Universidad de la Republica; UruguayFil: Varela, Adriana. Uruguay. Ministerio de Salud Pública; UruguayFil: Algorta, Gabriela. Universidad de la Republica; Uruguay. Uruguay. Ministerio de Salud Pública; UruguayFil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Ayala, Juan A.. Universidad Autónoma de Madrid; España. Consejo Superior de Investigaciones Cientificas; EspañaFil: Vignoli, Rafael. Universidad de la Republica; UruguayOxford University Press2011-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/12923García Fulgueiras, Virginia; Bado, Inés; Mota, María Inés; Robino, Luciana; Cordeiro, Nicolás F.; et al.; Extended-spectrum β-lactamases and plasmid-mediated quinolone resistance in enterobacterial clinical isolates in the paediatric hospital of Uruguay; Oxford University Press; Journal of Antimicrobial Chemotherapy; 66; 8; 8-2011; 1725-17290305-7453enginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dkr222info:eu-repo/semantics/altIdentifier/doi/10.1093/jac/dkr222info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:15Zoai:ri.conicet.gov.ar:11336/12923instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:16.243CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Extended-spectrum β-lactamases and plasmid-mediated quinolone resistance in enterobacterial clinical isolates in the paediatric hospital of Uruguay
title Extended-spectrum β-lactamases and plasmid-mediated quinolone resistance in enterobacterial clinical isolates in the paediatric hospital of Uruguay
spellingShingle Extended-spectrum β-lactamases and plasmid-mediated quinolone resistance in enterobacterial clinical isolates in the paediatric hospital of Uruguay
García Fulgueiras, Virginia
Antibiotic Resistance
Enterobacteriaceae
Integrons
title_short Extended-spectrum β-lactamases and plasmid-mediated quinolone resistance in enterobacterial clinical isolates in the paediatric hospital of Uruguay
title_full Extended-spectrum β-lactamases and plasmid-mediated quinolone resistance in enterobacterial clinical isolates in the paediatric hospital of Uruguay
title_fullStr Extended-spectrum β-lactamases and plasmid-mediated quinolone resistance in enterobacterial clinical isolates in the paediatric hospital of Uruguay
title_full_unstemmed Extended-spectrum β-lactamases and plasmid-mediated quinolone resistance in enterobacterial clinical isolates in the paediatric hospital of Uruguay
title_sort Extended-spectrum β-lactamases and plasmid-mediated quinolone resistance in enterobacterial clinical isolates in the paediatric hospital of Uruguay
dc.creator.none.fl_str_mv García Fulgueiras, Virginia
Bado, Inés
Mota, María Inés
Robino, Luciana
Cordeiro, Nicolás F.
Varela, Adriana
Algorta, Gabriela
Gutkind, Gabriel Osvaldo
Ayala, Juan A.
Vignoli, Rafael
author García Fulgueiras, Virginia
author_facet García Fulgueiras, Virginia
Bado, Inés
Mota, María Inés
Robino, Luciana
Cordeiro, Nicolás F.
Varela, Adriana
Algorta, Gabriela
Gutkind, Gabriel Osvaldo
Ayala, Juan A.
Vignoli, Rafael
author_role author
author2 Bado, Inés
Mota, María Inés
Robino, Luciana
Cordeiro, Nicolás F.
Varela, Adriana
Algorta, Gabriela
Gutkind, Gabriel Osvaldo
Ayala, Juan A.
Vignoli, Rafael
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Antibiotic Resistance
Enterobacteriaceae
Integrons
topic Antibiotic Resistance
Enterobacteriaceae
Integrons
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Objectives To analyse the prevalence of resistance to β-lactams and plasmid-mediated quinolone resistance in Enterobacteriaceae in the paediatric hospital of Uruguay. Methods A total of 368 enterobacterial isolates collected between 1 May and 30 November 2009 were studied for the presence of extended-spectrum β-lactamases (ESBLs), qnr alleles and aac(6′)Ib by phenotypic and molecular methods. The genomic context and transferability of β-lactamase and qnr genes were examined by PCR and conjugation, respectively. Results The proportion of inpatients having an infection caused by ESBL-producing enterobacteria was 0.23% (16/7073) in paediatrics wards, 0.64‰ (3/4696) in the neonatology department and 0.03‰ (1/32 557) in the emergency department. ESBL-carrying enterobacteria constituted a total of 21.6% (16/74), 13% (3/23) and 0.37% (1/271) when samples were obtained from paediatrics wards, the neonatology department and the emergency department, respectively. Overall, CTX-M-2 (n = 7), CTX-M-9 (n = 3), CTX-M-8 (n = 2), CTX-M-15 (n = 1), SHV-5 (n = 5) and SHV-2 (n = 2) β-lactamases were detected. Thirteen out of 20 ESBL-producing isolates also carried the aac(6′)Ib gene, and the cr variant was detected in one of them. qnr alleles were detected in four isolates comprising two qnrA1 genes, a qnrB8-like variant and a new qnrB gene showing 26 amino acid differences from QnrB1. Conclusions The proportion of ESBL-producing enterobacteria in Uruguay's paediatric hospital during the study period was 2.3 per 1000 hospitalized patients. The number of different microorganisms detected, as well as the various EBSLs, suggests the occurrence of sporadic episodes instead of nosocomial outbreaks. Nevertheless, the presence of new resistance genes reinforces the necessity for permanent surveillance programmes.
Fil: García Fulgueiras, Virginia. Universidad de la Republica; Uruguay
Fil: Bado, Inés. Universidad de la Republica; Uruguay
Fil: Mota, María Inés. Universidad de la Republica; Uruguay. Uruguay. Ministerio de Salud Pública; Uruguay
Fil: Robino, Luciana. Universidad de la Republica; Uruguay
Fil: Cordeiro, Nicolás F.. Universidad de la Republica; Uruguay
Fil: Varela, Adriana. Uruguay. Ministerio de Salud Pública; Uruguay
Fil: Algorta, Gabriela. Universidad de la Republica; Uruguay. Uruguay. Ministerio de Salud Pública; Uruguay
Fil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Ayala, Juan A.. Universidad Autónoma de Madrid; España. Consejo Superior de Investigaciones Cientificas; España
Fil: Vignoli, Rafael. Universidad de la Republica; Uruguay
description Objectives To analyse the prevalence of resistance to β-lactams and plasmid-mediated quinolone resistance in Enterobacteriaceae in the paediatric hospital of Uruguay. Methods A total of 368 enterobacterial isolates collected between 1 May and 30 November 2009 were studied for the presence of extended-spectrum β-lactamases (ESBLs), qnr alleles and aac(6′)Ib by phenotypic and molecular methods. The genomic context and transferability of β-lactamase and qnr genes were examined by PCR and conjugation, respectively. Results The proportion of inpatients having an infection caused by ESBL-producing enterobacteria was 0.23% (16/7073) in paediatrics wards, 0.64‰ (3/4696) in the neonatology department and 0.03‰ (1/32 557) in the emergency department. ESBL-carrying enterobacteria constituted a total of 21.6% (16/74), 13% (3/23) and 0.37% (1/271) when samples were obtained from paediatrics wards, the neonatology department and the emergency department, respectively. Overall, CTX-M-2 (n = 7), CTX-M-9 (n = 3), CTX-M-8 (n = 2), CTX-M-15 (n = 1), SHV-5 (n = 5) and SHV-2 (n = 2) β-lactamases were detected. Thirteen out of 20 ESBL-producing isolates also carried the aac(6′)Ib gene, and the cr variant was detected in one of them. qnr alleles were detected in four isolates comprising two qnrA1 genes, a qnrB8-like variant and a new qnrB gene showing 26 amino acid differences from QnrB1. Conclusions The proportion of ESBL-producing enterobacteria in Uruguay's paediatric hospital during the study period was 2.3 per 1000 hospitalized patients. The number of different microorganisms detected, as well as the various EBSLs, suggests the occurrence of sporadic episodes instead of nosocomial outbreaks. Nevertheless, the presence of new resistance genes reinforces the necessity for permanent surveillance programmes.
publishDate 2011
dc.date.none.fl_str_mv 2011-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/12923
García Fulgueiras, Virginia; Bado, Inés; Mota, María Inés; Robino, Luciana; Cordeiro, Nicolás F.; et al.; Extended-spectrum β-lactamases and plasmid-mediated quinolone resistance in enterobacterial clinical isolates in the paediatric hospital of Uruguay; Oxford University Press; Journal of Antimicrobial Chemotherapy; 66; 8; 8-2011; 1725-1729
0305-7453
url http://hdl.handle.net/11336/12923
identifier_str_mv García Fulgueiras, Virginia; Bado, Inés; Mota, María Inés; Robino, Luciana; Cordeiro, Nicolás F.; et al.; Extended-spectrum β-lactamases and plasmid-mediated quinolone resistance in enterobacterial clinical isolates in the paediatric hospital of Uruguay; Oxford University Press; Journal of Antimicrobial Chemotherapy; 66; 8; 8-2011; 1725-1729
0305-7453
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dkr222
info:eu-repo/semantics/altIdentifier/doi/10.1093/jac/dkr222
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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