Imidacloprid Causes DNA Damage in Fish: Clastogenesis as a Mechanism of Genotoxicity

Autores
Iturburu, Fernando Gastón; Simoniello, Maria Fernanda; Medici, Sandra Karina; Panzeri, Ana M.; Menone, Mirta Lujan
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Neonicotinoids are one of the most widely used insecticides in the world. DNA damage is considered an early biological effect which could lead to reproductive and carcinogenic effects. The present study aimed to evaluate DNA damage and bases oxidation as a mechanism of genotoxicity, on the freshwater fish Australoheros facetus acutely exposed to imidacloprid (IMI). The Comet assay with the nuclease ENDO III enzyme was performed for detecting pyrimidine bases oxidation using blood samples. Micronucleus and other nuclear abnormalities frequencies were also quantified. A significant increase of damage index at 100 and 1000 µg/L IMI was detected; while ENDO III score increased from 1 to 1000 µg/L IMI; varying both in a linear concentration-response manner. MN frequency increased in fish exposed to 1000 µg/L IMI. These results show that short-term exposures to environmentally relevant concentrations of IMI could affect the genetic integrity of fishes through oxidative damage.
Fil: Iturburu, Fernando Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Simoniello, Maria Fernanda. Universidad Nacional del Litoral; Argentina
Fil: Medici, Sandra Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Panzeri, Ana M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Menone, Mirta Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Materia
Neonicotinoid
Fishes
DNA fragmentation
Micronucleus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/92397
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Imidacloprid Causes DNA Damage in Fish: Clastogenesis as a Mechanism of GenotoxicityIturburu, Fernando GastónSimoniello, Maria FernandaMedici, Sandra KarinaPanzeri, Ana M.Menone, Mirta LujanNeonicotinoidFishesDNA fragmentationMicronucleushttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Neonicotinoids are one of the most widely used insecticides in the world. DNA damage is considered an early biological effect which could lead to reproductive and carcinogenic effects. The present study aimed to evaluate DNA damage and bases oxidation as a mechanism of genotoxicity, on the freshwater fish Australoheros facetus acutely exposed to imidacloprid (IMI). The Comet assay with the nuclease ENDO III enzyme was performed for detecting pyrimidine bases oxidation using blood samples. Micronucleus and other nuclear abnormalities frequencies were also quantified. A significant increase of damage index at 100 and 1000 µg/L IMI was detected; while ENDO III score increased from 1 to 1000 µg/L IMI; varying both in a linear concentration-response manner. MN frequency increased in fish exposed to 1000 µg/L IMI. These results show that short-term exposures to environmentally relevant concentrations of IMI could affect the genetic integrity of fishes through oxidative damage.Fil: Iturburu, Fernando Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Simoniello, Maria Fernanda. Universidad Nacional del Litoral; ArgentinaFil: Medici, Sandra Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Panzeri, Ana M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Menone, Mirta Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaSpringer2018-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/92397Iturburu, Fernando Gastón; Simoniello, Maria Fernanda; Medici, Sandra Karina; Panzeri, Ana M.; Menone, Mirta Lujan; Imidacloprid Causes DNA Damage in Fish: Clastogenesis as a Mechanism of Genotoxicity; Springer; Bulletin of Environmental Contamination and Toxicology; 100; 6; 4-2018; 760-7640007-4861CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s00128-018-2338-0info:eu-repo/semantics/altIdentifier/doi/10.1007/s00128-018-2338-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:09Zoai:ri.conicet.gov.ar:11336/92397instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:09.886CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Imidacloprid Causes DNA Damage in Fish: Clastogenesis as a Mechanism of Genotoxicity
title Imidacloprid Causes DNA Damage in Fish: Clastogenesis as a Mechanism of Genotoxicity
spellingShingle Imidacloprid Causes DNA Damage in Fish: Clastogenesis as a Mechanism of Genotoxicity
Iturburu, Fernando Gastón
Neonicotinoid
Fishes
DNA fragmentation
Micronucleus
title_short Imidacloprid Causes DNA Damage in Fish: Clastogenesis as a Mechanism of Genotoxicity
title_full Imidacloprid Causes DNA Damage in Fish: Clastogenesis as a Mechanism of Genotoxicity
title_fullStr Imidacloprid Causes DNA Damage in Fish: Clastogenesis as a Mechanism of Genotoxicity
title_full_unstemmed Imidacloprid Causes DNA Damage in Fish: Clastogenesis as a Mechanism of Genotoxicity
title_sort Imidacloprid Causes DNA Damage in Fish: Clastogenesis as a Mechanism of Genotoxicity
dc.creator.none.fl_str_mv Iturburu, Fernando Gastón
Simoniello, Maria Fernanda
Medici, Sandra Karina
Panzeri, Ana M.
Menone, Mirta Lujan
author Iturburu, Fernando Gastón
author_facet Iturburu, Fernando Gastón
Simoniello, Maria Fernanda
Medici, Sandra Karina
Panzeri, Ana M.
Menone, Mirta Lujan
author_role author
author2 Simoniello, Maria Fernanda
Medici, Sandra Karina
Panzeri, Ana M.
Menone, Mirta Lujan
author2_role author
author
author
author
dc.subject.none.fl_str_mv Neonicotinoid
Fishes
DNA fragmentation
Micronucleus
topic Neonicotinoid
Fishes
DNA fragmentation
Micronucleus
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Neonicotinoids are one of the most widely used insecticides in the world. DNA damage is considered an early biological effect which could lead to reproductive and carcinogenic effects. The present study aimed to evaluate DNA damage and bases oxidation as a mechanism of genotoxicity, on the freshwater fish Australoheros facetus acutely exposed to imidacloprid (IMI). The Comet assay with the nuclease ENDO III enzyme was performed for detecting pyrimidine bases oxidation using blood samples. Micronucleus and other nuclear abnormalities frequencies were also quantified. A significant increase of damage index at 100 and 1000 µg/L IMI was detected; while ENDO III score increased from 1 to 1000 µg/L IMI; varying both in a linear concentration-response manner. MN frequency increased in fish exposed to 1000 µg/L IMI. These results show that short-term exposures to environmentally relevant concentrations of IMI could affect the genetic integrity of fishes through oxidative damage.
Fil: Iturburu, Fernando Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Simoniello, Maria Fernanda. Universidad Nacional del Litoral; Argentina
Fil: Medici, Sandra Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Panzeri, Ana M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Menone, Mirta Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
description Neonicotinoids are one of the most widely used insecticides in the world. DNA damage is considered an early biological effect which could lead to reproductive and carcinogenic effects. The present study aimed to evaluate DNA damage and bases oxidation as a mechanism of genotoxicity, on the freshwater fish Australoheros facetus acutely exposed to imidacloprid (IMI). The Comet assay with the nuclease ENDO III enzyme was performed for detecting pyrimidine bases oxidation using blood samples. Micronucleus and other nuclear abnormalities frequencies were also quantified. A significant increase of damage index at 100 and 1000 µg/L IMI was detected; while ENDO III score increased from 1 to 1000 µg/L IMI; varying both in a linear concentration-response manner. MN frequency increased in fish exposed to 1000 µg/L IMI. These results show that short-term exposures to environmentally relevant concentrations of IMI could affect the genetic integrity of fishes through oxidative damage.
publishDate 2018
dc.date.none.fl_str_mv 2018-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/92397
Iturburu, Fernando Gastón; Simoniello, Maria Fernanda; Medici, Sandra Karina; Panzeri, Ana M.; Menone, Mirta Lujan; Imidacloprid Causes DNA Damage in Fish: Clastogenesis as a Mechanism of Genotoxicity; Springer; Bulletin of Environmental Contamination and Toxicology; 100; 6; 4-2018; 760-764
0007-4861
CONICET Digital
CONICET
url http://hdl.handle.net/11336/92397
identifier_str_mv Iturburu, Fernando Gastón; Simoniello, Maria Fernanda; Medici, Sandra Karina; Panzeri, Ana M.; Menone, Mirta Lujan; Imidacloprid Causes DNA Damage in Fish: Clastogenesis as a Mechanism of Genotoxicity; Springer; Bulletin of Environmental Contamination and Toxicology; 100; 6; 4-2018; 760-764
0007-4861
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/10.1007/s00128-018-2338-0
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00128-018-2338-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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