Pirimicarb-based formulation-induced genotoxicity and cytotoxicity on the fresh water fish Cnesterodon decemmaculatus (Jenyns, 1842) (Pisces, Poeciliidae)

Autores
Vera Candioti, Josefina; Soloneski, Sonia Maria Elsa; Larramendy, Marcelo Luis
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We analyzed the aspects of lethality, genotoxicity, and cytotoxicity in the ten spotted live-bearer exposed under laboratory conditions to the pirimicarb-based formulation Patton Flow1 (50% active ingredient (a.i.)). Acute effects were evaluated using different end points for lethality, genotoxicity, and cytotoxicity. Median lethal concentration (LC50) estimation was employed as a bioassay for lethality, whereas micronucleus (MN) induction and alterations in erythrocyte/erythroblast frequency were used as end points for genotoxicity and cytotoxicity, respectively. Results demonstrated an LC5096h value of 88 mg/L. Patton Flow1 increased the MN frequency in fish erythrocytes after 48 h of exposure at a concentration of 66 mg/L, whereas a concentration range of 22?66 mg/L was able to exert the same genotoxic effect at 96 h of treatment. Furthermore, cytotoxicity was also observed by alterations in erythrocyte/erythroblast frequencies within the concentration range of 22? 66 mg/L, regardless of the exposure time. Our current observations provide evidence that Patton Flow1 (50% a.i.) should be considered a clear lethal, cytotoxic, and genotoxic agent on Cnesterodon decemmaculatus. Thus, repeated applications of this carbamic insecticide can enter the aquatic environment and exert deleterious effects on aquatic organisms other than the evaluated species C. decemmaculatus.
Fil: Vera Candioti, Josefina. Universidad Nacional de la Plata. Facultad de Cs.naturales y Museo. Laboratorio de Citogenetica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Soloneski, Sonia Maria Elsa. Universidad Nacional de la Plata. Facultad de Cs.naturales y Museo. Laboratorio de Citogenetica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Larramendy, Marcelo Luis. Universidad Nacional de la Plata. Facultad de Cs.naturales y Museo. Laboratorio de Citogenetica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Materia
Patton Flow
Erythrocyte
Erythroblast Ratio
Micronucleus
Mortality
Poeciliidae
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/19171
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/19171
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Pirimicarb-based formulation-induced genotoxicity and cytotoxicity on the fresh water fish Cnesterodon decemmaculatus (Jenyns, 1842) (Pisces, Poeciliidae)Vera Candioti, JosefinaSoloneski, Sonia Maria ElsaLarramendy, Marcelo LuisPatton FlowErythrocyteErythroblast RatioMicronucleusMortalityPoeciliidaehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We analyzed the aspects of lethality, genotoxicity, and cytotoxicity in the ten spotted live-bearer exposed under laboratory conditions to the pirimicarb-based formulation Patton Flow1 (50% active ingredient (a.i.)). Acute effects were evaluated using different end points for lethality, genotoxicity, and cytotoxicity. Median lethal concentration (LC50) estimation was employed as a bioassay for lethality, whereas micronucleus (MN) induction and alterations in erythrocyte/erythroblast frequency were used as end points for genotoxicity and cytotoxicity, respectively. Results demonstrated an LC5096h value of 88 mg/L. Patton Flow1 increased the MN frequency in fish erythrocytes after 48 h of exposure at a concentration of 66 mg/L, whereas a concentration range of 22?66 mg/L was able to exert the same genotoxic effect at 96 h of treatment. Furthermore, cytotoxicity was also observed by alterations in erythrocyte/erythroblast frequencies within the concentration range of 22? 66 mg/L, regardless of the exposure time. Our current observations provide evidence that Patton Flow1 (50% a.i.) should be considered a clear lethal, cytotoxic, and genotoxic agent on Cnesterodon decemmaculatus. Thus, repeated applications of this carbamic insecticide can enter the aquatic environment and exert deleterious effects on aquatic organisms other than the evaluated species C. decemmaculatus.Fil: Vera Candioti, Josefina. Universidad Nacional de la Plata. Facultad de Cs.naturales y Museo. Laboratorio de Citogenetica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Soloneski, Sonia Maria Elsa. Universidad Nacional de la Plata. Facultad de Cs.naturales y Museo. Laboratorio de Citogenetica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Larramendy, Marcelo Luis. Universidad Nacional de la Plata. Facultad de Cs.naturales y Museo. Laboratorio de Citogenetica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaSage Publications Ltd2015-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/19171Vera Candioti, Josefina; Soloneski, Sonia Maria Elsa; Larramendy, Marcelo Luis; Pirimicarb-based formulation-induced genotoxicity and cytotoxicity on the fresh water fish Cnesterodon decemmaculatus (Jenyns, 1842) (Pisces, Poeciliidae); Sage Publications Ltd; Toxicology And Industrial Health; 31; 11; 3-2015; 1051-10600748-2337CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1177/0748233713486958info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/10.1177/0748233713486958info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:49Zoai:ri.conicet.gov.ar:11336/19171instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:49.292CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Pirimicarb-based formulation-induced genotoxicity and cytotoxicity on the fresh water fish Cnesterodon decemmaculatus (Jenyns, 1842) (Pisces, Poeciliidae)
title Pirimicarb-based formulation-induced genotoxicity and cytotoxicity on the fresh water fish Cnesterodon decemmaculatus (Jenyns, 1842) (Pisces, Poeciliidae)
spellingShingle Pirimicarb-based formulation-induced genotoxicity and cytotoxicity on the fresh water fish Cnesterodon decemmaculatus (Jenyns, 1842) (Pisces, Poeciliidae)
Vera Candioti, Josefina
Patton Flow
Erythrocyte
Erythroblast Ratio
Micronucleus
Mortality
Poeciliidae
title_short Pirimicarb-based formulation-induced genotoxicity and cytotoxicity on the fresh water fish Cnesterodon decemmaculatus (Jenyns, 1842) (Pisces, Poeciliidae)
title_full Pirimicarb-based formulation-induced genotoxicity and cytotoxicity on the fresh water fish Cnesterodon decemmaculatus (Jenyns, 1842) (Pisces, Poeciliidae)
title_fullStr Pirimicarb-based formulation-induced genotoxicity and cytotoxicity on the fresh water fish Cnesterodon decemmaculatus (Jenyns, 1842) (Pisces, Poeciliidae)
title_full_unstemmed Pirimicarb-based formulation-induced genotoxicity and cytotoxicity on the fresh water fish Cnesterodon decemmaculatus (Jenyns, 1842) (Pisces, Poeciliidae)
title_sort Pirimicarb-based formulation-induced genotoxicity and cytotoxicity on the fresh water fish Cnesterodon decemmaculatus (Jenyns, 1842) (Pisces, Poeciliidae)
dc.creator.none.fl_str_mv Vera Candioti, Josefina
Soloneski, Sonia Maria Elsa
Larramendy, Marcelo Luis
author Vera Candioti, Josefina
author_facet Vera Candioti, Josefina
Soloneski, Sonia Maria Elsa
Larramendy, Marcelo Luis
author_role author
author2 Soloneski, Sonia Maria Elsa
Larramendy, Marcelo Luis
author2_role author
author
dc.subject.none.fl_str_mv Patton Flow
Erythrocyte
Erythroblast Ratio
Micronucleus
Mortality
Poeciliidae
topic Patton Flow
Erythrocyte
Erythroblast Ratio
Micronucleus
Mortality
Poeciliidae
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We analyzed the aspects of lethality, genotoxicity, and cytotoxicity in the ten spotted live-bearer exposed under laboratory conditions to the pirimicarb-based formulation Patton Flow1 (50% active ingredient (a.i.)). Acute effects were evaluated using different end points for lethality, genotoxicity, and cytotoxicity. Median lethal concentration (LC50) estimation was employed as a bioassay for lethality, whereas micronucleus (MN) induction and alterations in erythrocyte/erythroblast frequency were used as end points for genotoxicity and cytotoxicity, respectively. Results demonstrated an LC5096h value of 88 mg/L. Patton Flow1 increased the MN frequency in fish erythrocytes after 48 h of exposure at a concentration of 66 mg/L, whereas a concentration range of 22?66 mg/L was able to exert the same genotoxic effect at 96 h of treatment. Furthermore, cytotoxicity was also observed by alterations in erythrocyte/erythroblast frequencies within the concentration range of 22? 66 mg/L, regardless of the exposure time. Our current observations provide evidence that Patton Flow1 (50% a.i.) should be considered a clear lethal, cytotoxic, and genotoxic agent on Cnesterodon decemmaculatus. Thus, repeated applications of this carbamic insecticide can enter the aquatic environment and exert deleterious effects on aquatic organisms other than the evaluated species C. decemmaculatus.
Fil: Vera Candioti, Josefina. Universidad Nacional de la Plata. Facultad de Cs.naturales y Museo. Laboratorio de Citogenetica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Soloneski, Sonia Maria Elsa. Universidad Nacional de la Plata. Facultad de Cs.naturales y Museo. Laboratorio de Citogenetica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Larramendy, Marcelo Luis. Universidad Nacional de la Plata. Facultad de Cs.naturales y Museo. Laboratorio de Citogenetica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
description We analyzed the aspects of lethality, genotoxicity, and cytotoxicity in the ten spotted live-bearer exposed under laboratory conditions to the pirimicarb-based formulation Patton Flow1 (50% active ingredient (a.i.)). Acute effects were evaluated using different end points for lethality, genotoxicity, and cytotoxicity. Median lethal concentration (LC50) estimation was employed as a bioassay for lethality, whereas micronucleus (MN) induction and alterations in erythrocyte/erythroblast frequency were used as end points for genotoxicity and cytotoxicity, respectively. Results demonstrated an LC5096h value of 88 mg/L. Patton Flow1 increased the MN frequency in fish erythrocytes after 48 h of exposure at a concentration of 66 mg/L, whereas a concentration range of 22?66 mg/L was able to exert the same genotoxic effect at 96 h of treatment. Furthermore, cytotoxicity was also observed by alterations in erythrocyte/erythroblast frequencies within the concentration range of 22? 66 mg/L, regardless of the exposure time. Our current observations provide evidence that Patton Flow1 (50% a.i.) should be considered a clear lethal, cytotoxic, and genotoxic agent on Cnesterodon decemmaculatus. Thus, repeated applications of this carbamic insecticide can enter the aquatic environment and exert deleterious effects on aquatic organisms other than the evaluated species C. decemmaculatus.
publishDate 2015
dc.date.none.fl_str_mv 2015-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/19171
Vera Candioti, Josefina; Soloneski, Sonia Maria Elsa; Larramendy, Marcelo Luis; Pirimicarb-based formulation-induced genotoxicity and cytotoxicity on the fresh water fish Cnesterodon decemmaculatus (Jenyns, 1842) (Pisces, Poeciliidae); Sage Publications Ltd; Toxicology And Industrial Health; 31; 11; 3-2015; 1051-1060
0748-2337
CONICET Digital
CONICET
url http://hdl.handle.net/11336/19171
identifier_str_mv Vera Candioti, Josefina; Soloneski, Sonia Maria Elsa; Larramendy, Marcelo Luis; Pirimicarb-based formulation-induced genotoxicity and cytotoxicity on the fresh water fish Cnesterodon decemmaculatus (Jenyns, 1842) (Pisces, Poeciliidae); Sage Publications Ltd; Toxicology And Industrial Health; 31; 11; 3-2015; 1051-1060
0748-2337
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1177/0748233713486958
info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/10.1177/0748233713486958
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sage Publications Ltd
publisher.none.fl_str_mv Sage Publications Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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