Effect of the 3,5,3'-Triiodo-L-Thyronine on Zwitterionic and Anionic Membranes

Autores
Sosa Morales, Marcelo Clemente; Alvarez, Rosa Maria Susana; Montich, Guillermo Gabriel
Año de publicación
2012
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Vibrational spectroscopy is a powerful tool for understanding interactions between phospholipid membranes and molecules of biological relevance such as thyroid hormones. Particularly, 3,5,3´-triiodo L-thyronine (T3) has a wide range of effects on metabolism and development. The present work consists in the analysis by FT-IR and Raman spectroscopies of the influence of T3 on the conformation of the model membranes. We measured the FT-IR spectra of dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylglycerol (DPPG) as function of the temperature, in absence and presence of T3 bound to these model membranes. In order to compare the phase transition behaviors, monitoring of the frequency shifts of the antisymmetric methylene stretching vibration near 2920 cm-1 was performed. The results demonstrate a large decrease in the transition temperature (Tm) from the gel to the liquid crystalline state when T3 is bound to DPPG (between 10-15 °C) while DPPC shows a decrease between 1-2°C upon the bound hormone. On the other hand, spectral Raman indicators have been used to the determination of acyl chain coupling and conformational order upon hormone incorporation to the model membranes. Particularly, the I1096/I1129 peak intensity ratio, indicative of the gauche:trans conformers proportion, was evaluated by Confocal Raman Microscopy. Spectra of the lipids in gel phase were obtained at room temperature. Differences in the gauche conformer´s content, induced by T3, suggest the existence of distinct interactions of the hormone with the membrane, according this is anionic or zwitterionic.
Fil: Sosa Morales, Marcelo Clemente. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Alvarez, Rosa Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Montich, Guillermo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
XLI Reunión Anual de la Sociedad Argentina de Biofísica
San Javier
Argentina
Sociedad Argentina de Biofísica
Materia
THYROID HORMONES
RAMAN
FTIR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/258234
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/258234
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of the 3,5,3'-Triiodo-L-Thyronine on Zwitterionic and Anionic MembranesSosa Morales, Marcelo ClementeAlvarez, Rosa Maria SusanaMontich, Guillermo GabrielTHYROID HORMONESRAMANFTIRhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Vibrational spectroscopy is a powerful tool for understanding interactions between phospholipid membranes and molecules of biological relevance such as thyroid hormones. Particularly, 3,5,3´-triiodo L-thyronine (T3) has a wide range of effects on metabolism and development. The present work consists in the analysis by FT-IR and Raman spectroscopies of the influence of T3 on the conformation of the model membranes. We measured the FT-IR spectra of dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylglycerol (DPPG) as function of the temperature, in absence and presence of T3 bound to these model membranes. In order to compare the phase transition behaviors, monitoring of the frequency shifts of the antisymmetric methylene stretching vibration near 2920 cm-1 was performed. The results demonstrate a large decrease in the transition temperature (Tm) from the gel to the liquid crystalline state when T3 is bound to DPPG (between 10-15 °C) while DPPC shows a decrease between 1-2°C upon the bound hormone. On the other hand, spectral Raman indicators have been used to the determination of acyl chain coupling and conformational order upon hormone incorporation to the model membranes. Particularly, the I1096/I1129 peak intensity ratio, indicative of the gauche:trans conformers proportion, was evaluated by Confocal Raman Microscopy. Spectra of the lipids in gel phase were obtained at room temperature. Differences in the gauche conformer´s content, induced by T3, suggest the existence of distinct interactions of the hormone with the membrane, according this is anionic or zwitterionic.Fil: Sosa Morales, Marcelo Clemente. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Alvarez, Rosa Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Montich, Guillermo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaXLI Reunión Anual de la Sociedad Argentina de BiofísicaSan JavierArgentinaSociedad Argentina de BiofísicaSociedad Argentina de Biofísica2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/258234Effect of the 3,5,3'-Triiodo-L-Thyronine on Zwitterionic and Anionic Membranes; XLI Reunión Anual de la Sociedad Argentina de Biofísica; San Javier; Argentina; 2012; 200-200978-987-27591-1-7CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://biofisica.org.ar/publicaciones/libros-de-resumenes/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:00:27Zoai:ri.conicet.gov.ar:11336/258234instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:00:28.131CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of the 3,5,3'-Triiodo-L-Thyronine on Zwitterionic and Anionic Membranes
title Effect of the 3,5,3'-Triiodo-L-Thyronine on Zwitterionic and Anionic Membranes
spellingShingle Effect of the 3,5,3'-Triiodo-L-Thyronine on Zwitterionic and Anionic Membranes
Sosa Morales, Marcelo Clemente
THYROID HORMONES
RAMAN
FTIR
title_short Effect of the 3,5,3'-Triiodo-L-Thyronine on Zwitterionic and Anionic Membranes
title_full Effect of the 3,5,3'-Triiodo-L-Thyronine on Zwitterionic and Anionic Membranes
title_fullStr Effect of the 3,5,3'-Triiodo-L-Thyronine on Zwitterionic and Anionic Membranes
title_full_unstemmed Effect of the 3,5,3'-Triiodo-L-Thyronine on Zwitterionic and Anionic Membranes
title_sort Effect of the 3,5,3'-Triiodo-L-Thyronine on Zwitterionic and Anionic Membranes
dc.creator.none.fl_str_mv Sosa Morales, Marcelo Clemente
Alvarez, Rosa Maria Susana
Montich, Guillermo Gabriel
author Sosa Morales, Marcelo Clemente
author_facet Sosa Morales, Marcelo Clemente
Alvarez, Rosa Maria Susana
Montich, Guillermo Gabriel
author_role author
author2 Alvarez, Rosa Maria Susana
Montich, Guillermo Gabriel
author2_role author
author
dc.subject.none.fl_str_mv THYROID HORMONES
RAMAN
FTIR
topic THYROID HORMONES
RAMAN
FTIR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Vibrational spectroscopy is a powerful tool for understanding interactions between phospholipid membranes and molecules of biological relevance such as thyroid hormones. Particularly, 3,5,3´-triiodo L-thyronine (T3) has a wide range of effects on metabolism and development. The present work consists in the analysis by FT-IR and Raman spectroscopies of the influence of T3 on the conformation of the model membranes. We measured the FT-IR spectra of dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylglycerol (DPPG) as function of the temperature, in absence and presence of T3 bound to these model membranes. In order to compare the phase transition behaviors, monitoring of the frequency shifts of the antisymmetric methylene stretching vibration near 2920 cm-1 was performed. The results demonstrate a large decrease in the transition temperature (Tm) from the gel to the liquid crystalline state when T3 is bound to DPPG (between 10-15 °C) while DPPC shows a decrease between 1-2°C upon the bound hormone. On the other hand, spectral Raman indicators have been used to the determination of acyl chain coupling and conformational order upon hormone incorporation to the model membranes. Particularly, the I1096/I1129 peak intensity ratio, indicative of the gauche:trans conformers proportion, was evaluated by Confocal Raman Microscopy. Spectra of the lipids in gel phase were obtained at room temperature. Differences in the gauche conformer´s content, induced by T3, suggest the existence of distinct interactions of the hormone with the membrane, according this is anionic or zwitterionic.
Fil: Sosa Morales, Marcelo Clemente. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Alvarez, Rosa Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Montich, Guillermo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
XLI Reunión Anual de la Sociedad Argentina de Biofísica
San Javier
Argentina
Sociedad Argentina de Biofísica
description Vibrational spectroscopy is a powerful tool for understanding interactions between phospholipid membranes and molecules of biological relevance such as thyroid hormones. Particularly, 3,5,3´-triiodo L-thyronine (T3) has a wide range of effects on metabolism and development. The present work consists in the analysis by FT-IR and Raman spectroscopies of the influence of T3 on the conformation of the model membranes. We measured the FT-IR spectra of dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylglycerol (DPPG) as function of the temperature, in absence and presence of T3 bound to these model membranes. In order to compare the phase transition behaviors, monitoring of the frequency shifts of the antisymmetric methylene stretching vibration near 2920 cm-1 was performed. The results demonstrate a large decrease in the transition temperature (Tm) from the gel to the liquid crystalline state when T3 is bound to DPPG (between 10-15 °C) while DPPC shows a decrease between 1-2°C upon the bound hormone. On the other hand, spectral Raman indicators have been used to the determination of acyl chain coupling and conformational order upon hormone incorporation to the model membranes. Particularly, the I1096/I1129 peak intensity ratio, indicative of the gauche:trans conformers proportion, was evaluated by Confocal Raman Microscopy. Spectra of the lipids in gel phase were obtained at room temperature. Differences in the gauche conformer´s content, induced by T3, suggest the existence of distinct interactions of the hormone with the membrane, according this is anionic or zwitterionic.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/258234
Effect of the 3,5,3'-Triiodo-L-Thyronine on Zwitterionic and Anionic Membranes; XLI Reunión Anual de la Sociedad Argentina de Biofísica; San Javier; Argentina; 2012; 200-200
978-987-27591-1-7
CONICET Digital
CONICET
url http://hdl.handle.net/11336/258234
identifier_str_mv Effect of the 3,5,3'-Triiodo-L-Thyronine on Zwitterionic and Anionic Membranes; XLI Reunión Anual de la Sociedad Argentina de Biofísica; San Javier; Argentina; 2012; 200-200
978-987-27591-1-7
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://biofisica.org.ar/publicaciones/libros-de-resumenes/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Biofísica
publisher.none.fl_str_mv Sociedad Argentina de Biofísica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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