Prussian‐Blue Catalysis and NFC Synergy: a Battery‐Free Laser‐Induced Graphene‐Based Platform for Urine Glucose Monitoring at Point‐of‐Care

Autores
Uzunçar, Sinan; Maroli, Gabriel; Urban Massimo; Merkoçi Arben
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Prussian-blue nanoparticles (PBNPs) show promise in electrochemical hydrogen peroxide (H2O2) sensing but face operational stability challenges without complex strategies. This study introduces a simplified, polymer-based synthesis method, enhancing their stability in a single step. Chemical polymerization of Prussian-blue (PB) and poly(3,4-ethylenedioxythiophene) (PEDOT) with gelatin as a polycationic soft template yields a self-assembled PB-infused Catalytic Hetero-interface Architecture (PB-CHIA) that remarkably improves the stability of PBNPs and offers functional groups for enzyme immobilization, supporting robust biosensing applications. The softened PEDOT rigidity extends PB-CHIA´s applicability to various carbonaceous electrode substrates, including glassy carbon and laser-induced graphene (LIG) via simple drop-casting. A fluidic cell module designed with the optimized LIG morphology (nano-fibrous fringes, LIG-F, diameter: 72.87 ± 12.24 nm) modified with PB-CHIA and glucose oxidase enables non-invasive urine glucose monitoring. The configuration accurately quantifies glucose within a linear range of 10–400 µM [R2: 0.991, Sensitivity: 29.88 ± 4.98 µA mM⁻¹ cm⁻2, Detection Limit: 4.52 ± 2.24 µM], covering medical needs. A near-field communication potentiostat is devised for a fully integrated, batteryless, wireless point-of-care (POC) prototype, enabling rapid smartphone readouts in 15 s for daily home-based use. The stable operation of PB-CHIA allows working electrodes’ scalable production, highlighting its potential for diverse POC devices in urinary analysis reliant on H2O2 assays.
Fil: Uzunçar, Sinan. Institut Català de Nanociència I Nanotecnologia; España
Fil: Maroli, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones en Ingeniería Eléctrica "Alfredo Desages". Universidad Nacional del Sur. Departamento de Ingeniería Eléctrica y de Computadoras. Instituto de Investigaciones en Ingeniería Eléctrica "Alfredo Desages"; Argentina
Fil: Urban Massimo. Institut Català de Nanociència I Nanotecnologia; España
Fil: Merkoçi Arben. Institut Català de Nanociència I Nanotecnologia; España
Materia
GLUCOSE
BIOSENSOR
ELECTROCHEMISTRY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/279781
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Prussian‐Blue Catalysis and NFC Synergy: a Battery‐Free Laser‐Induced Graphene‐Based Platform for Urine Glucose Monitoring at Point‐of‐CareUzunçar, SinanMaroli, GabrielUrban MassimoMerkoçi ArbenGLUCOSEBIOSENSORELECTROCHEMISTRYhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Prussian-blue nanoparticles (PBNPs) show promise in electrochemical hydrogen peroxide (H2O2) sensing but face operational stability challenges without complex strategies. This study introduces a simplified, polymer-based synthesis method, enhancing their stability in a single step. Chemical polymerization of Prussian-blue (PB) and poly(3,4-ethylenedioxythiophene) (PEDOT) with gelatin as a polycationic soft template yields a self-assembled PB-infused Catalytic Hetero-interface Architecture (PB-CHIA) that remarkably improves the stability of PBNPs and offers functional groups for enzyme immobilization, supporting robust biosensing applications. The softened PEDOT rigidity extends PB-CHIA´s applicability to various carbonaceous electrode substrates, including glassy carbon and laser-induced graphene (LIG) via simple drop-casting. A fluidic cell module designed with the optimized LIG morphology (nano-fibrous fringes, LIG-F, diameter: 72.87 ± 12.24 nm) modified with PB-CHIA and glucose oxidase enables non-invasive urine glucose monitoring. The configuration accurately quantifies glucose within a linear range of 10–400 µM [R2: 0.991, Sensitivity: 29.88 ± 4.98 µA mM⁻¹ cm⁻2, Detection Limit: 4.52 ± 2.24 µM], covering medical needs. A near-field communication potentiostat is devised for a fully integrated, batteryless, wireless point-of-care (POC) prototype, enabling rapid smartphone readouts in 15 s for daily home-based use. The stable operation of PB-CHIA allows working electrodes’ scalable production, highlighting its potential for diverse POC devices in urinary analysis reliant on H2O2 assays.Fil: Uzunçar, Sinan. Institut Català de Nanociència I Nanotecnologia; EspañaFil: Maroli, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones en Ingeniería Eléctrica "Alfredo Desages". Universidad Nacional del Sur. Departamento de Ingeniería Eléctrica y de Computadoras. Instituto de Investigaciones en Ingeniería Eléctrica "Alfredo Desages"; ArgentinaFil: Urban Massimo. Institut Català de Nanociència I Nanotecnologia; EspañaFil: Merkoçi Arben. Institut Català de Nanociència I Nanotecnologia; EspañaWiley-VCH2025-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/279781Uzunçar, Sinan; Maroli, Gabriel; Urban Massimo; Merkoçi Arben; Prussian‐Blue Catalysis and NFC Synergy: a Battery‐Free Laser‐Induced Graphene‐Based Platform for Urine Glucose Monitoring at Point‐of‐Care; Wiley-VCH; Advanced Science; 12; 20; 3-2025; 1-172198-3844CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://advanced.onlinelibrary.wiley.com/doi/10.1002/advs.202500365info:eu-repo/semantics/altIdentifier/doi/10.1002/advs.202500365info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-26T10:11:04Zoai:ri.conicet.gov.ar:11336/279781instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-26 10:11:04.502CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Prussian‐Blue Catalysis and NFC Synergy: a Battery‐Free Laser‐Induced Graphene‐Based Platform for Urine Glucose Monitoring at Point‐of‐Care
title Prussian‐Blue Catalysis and NFC Synergy: a Battery‐Free Laser‐Induced Graphene‐Based Platform for Urine Glucose Monitoring at Point‐of‐Care
spellingShingle Prussian‐Blue Catalysis and NFC Synergy: a Battery‐Free Laser‐Induced Graphene‐Based Platform for Urine Glucose Monitoring at Point‐of‐Care
Uzunçar, Sinan
GLUCOSE
BIOSENSOR
ELECTROCHEMISTRY
title_short Prussian‐Blue Catalysis and NFC Synergy: a Battery‐Free Laser‐Induced Graphene‐Based Platform for Urine Glucose Monitoring at Point‐of‐Care
title_full Prussian‐Blue Catalysis and NFC Synergy: a Battery‐Free Laser‐Induced Graphene‐Based Platform for Urine Glucose Monitoring at Point‐of‐Care
title_fullStr Prussian‐Blue Catalysis and NFC Synergy: a Battery‐Free Laser‐Induced Graphene‐Based Platform for Urine Glucose Monitoring at Point‐of‐Care
title_full_unstemmed Prussian‐Blue Catalysis and NFC Synergy: a Battery‐Free Laser‐Induced Graphene‐Based Platform for Urine Glucose Monitoring at Point‐of‐Care
title_sort Prussian‐Blue Catalysis and NFC Synergy: a Battery‐Free Laser‐Induced Graphene‐Based Platform for Urine Glucose Monitoring at Point‐of‐Care
dc.creator.none.fl_str_mv Uzunçar, Sinan
Maroli, Gabriel
Urban Massimo
Merkoçi Arben
author Uzunçar, Sinan
author_facet Uzunçar, Sinan
Maroli, Gabriel
Urban Massimo
Merkoçi Arben
author_role author
author2 Maroli, Gabriel
Urban Massimo
Merkoçi Arben
author2_role author
author
author
dc.subject.none.fl_str_mv GLUCOSE
BIOSENSOR
ELECTROCHEMISTRY
topic GLUCOSE
BIOSENSOR
ELECTROCHEMISTRY
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Prussian-blue nanoparticles (PBNPs) show promise in electrochemical hydrogen peroxide (H2O2) sensing but face operational stability challenges without complex strategies. This study introduces a simplified, polymer-based synthesis method, enhancing their stability in a single step. Chemical polymerization of Prussian-blue (PB) and poly(3,4-ethylenedioxythiophene) (PEDOT) with gelatin as a polycationic soft template yields a self-assembled PB-infused Catalytic Hetero-interface Architecture (PB-CHIA) that remarkably improves the stability of PBNPs and offers functional groups for enzyme immobilization, supporting robust biosensing applications. The softened PEDOT rigidity extends PB-CHIA´s applicability to various carbonaceous electrode substrates, including glassy carbon and laser-induced graphene (LIG) via simple drop-casting. A fluidic cell module designed with the optimized LIG morphology (nano-fibrous fringes, LIG-F, diameter: 72.87 ± 12.24 nm) modified with PB-CHIA and glucose oxidase enables non-invasive urine glucose monitoring. The configuration accurately quantifies glucose within a linear range of 10–400 µM [R2: 0.991, Sensitivity: 29.88 ± 4.98 µA mM⁻¹ cm⁻2, Detection Limit: 4.52 ± 2.24 µM], covering medical needs. A near-field communication potentiostat is devised for a fully integrated, batteryless, wireless point-of-care (POC) prototype, enabling rapid smartphone readouts in 15 s for daily home-based use. The stable operation of PB-CHIA allows working electrodes’ scalable production, highlighting its potential for diverse POC devices in urinary analysis reliant on H2O2 assays.
Fil: Uzunçar, Sinan. Institut Català de Nanociència I Nanotecnologia; España
Fil: Maroli, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones en Ingeniería Eléctrica "Alfredo Desages". Universidad Nacional del Sur. Departamento de Ingeniería Eléctrica y de Computadoras. Instituto de Investigaciones en Ingeniería Eléctrica "Alfredo Desages"; Argentina
Fil: Urban Massimo. Institut Català de Nanociència I Nanotecnologia; España
Fil: Merkoçi Arben. Institut Català de Nanociència I Nanotecnologia; España
description Prussian-blue nanoparticles (PBNPs) show promise in electrochemical hydrogen peroxide (H2O2) sensing but face operational stability challenges without complex strategies. This study introduces a simplified, polymer-based synthesis method, enhancing their stability in a single step. Chemical polymerization of Prussian-blue (PB) and poly(3,4-ethylenedioxythiophene) (PEDOT) with gelatin as a polycationic soft template yields a self-assembled PB-infused Catalytic Hetero-interface Architecture (PB-CHIA) that remarkably improves the stability of PBNPs and offers functional groups for enzyme immobilization, supporting robust biosensing applications. The softened PEDOT rigidity extends PB-CHIA´s applicability to various carbonaceous electrode substrates, including glassy carbon and laser-induced graphene (LIG) via simple drop-casting. A fluidic cell module designed with the optimized LIG morphology (nano-fibrous fringes, LIG-F, diameter: 72.87 ± 12.24 nm) modified with PB-CHIA and glucose oxidase enables non-invasive urine glucose monitoring. The configuration accurately quantifies glucose within a linear range of 10–400 µM [R2: 0.991, Sensitivity: 29.88 ± 4.98 µA mM⁻¹ cm⁻2, Detection Limit: 4.52 ± 2.24 µM], covering medical needs. A near-field communication potentiostat is devised for a fully integrated, batteryless, wireless point-of-care (POC) prototype, enabling rapid smartphone readouts in 15 s for daily home-based use. The stable operation of PB-CHIA allows working electrodes’ scalable production, highlighting its potential for diverse POC devices in urinary analysis reliant on H2O2 assays.
publishDate 2025
dc.date.none.fl_str_mv 2025-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/279781
Uzunçar, Sinan; Maroli, Gabriel; Urban Massimo; Merkoçi Arben; Prussian‐Blue Catalysis and NFC Synergy: a Battery‐Free Laser‐Induced Graphene‐Based Platform for Urine Glucose Monitoring at Point‐of‐Care; Wiley-VCH; Advanced Science; 12; 20; 3-2025; 1-17
2198-3844
CONICET Digital
CONICET
url http://hdl.handle.net/11336/279781
identifier_str_mv Uzunçar, Sinan; Maroli, Gabriel; Urban Massimo; Merkoçi Arben; Prussian‐Blue Catalysis and NFC Synergy: a Battery‐Free Laser‐Induced Graphene‐Based Platform for Urine Glucose Monitoring at Point‐of‐Care; Wiley-VCH; Advanced Science; 12; 20; 3-2025; 1-17
2198-3844
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://advanced.onlinelibrary.wiley.com/doi/10.1002/advs.202500365
info:eu-repo/semantics/altIdentifier/doi/10.1002/advs.202500365
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-VCH
publisher.none.fl_str_mv Wiley-VCH
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.176822

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