Unleashing inkjet-printed nanostructured electrodes and battery-free potentiostat for the DNA-based multiplexed detection of SARS-CoV-2 genes

Autores
Rossetti, Marianna; Srisomwat, Chawin; Urban, Massimo; Rosati, Giulio; Maroli, Gabriel; Yaman Akbay, Hatice Gödze; Chailapakul, Orawon; Merkoçi, Arben
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Following the global COVID-19 pandemic triggered by SARS-CoV-2, the need for rapid, specific and cost-effective point-of-care diagnostic solutions remains paramount. Even though COVID-19 is no longer a public health emergency, the disease still poses a global threat leading to deaths, and it continues to change with the risk of new variants emerging causing a new surge in cases and deaths. Here, we address the urgent need for rapid, cost-effective and point-of-care diagnostic solutions for SARS-CoV-2. We propose a multiplexed DNA-based sensing platform that utilizes inkjet-printed nanostructured gold electrodes and an inkjet-printed battery-free near-field communication (NFC) potentiostat for the simultaneous quantitative detection of two SARS-CoV-2 genes, the ORF1ab and the N gene. The detection strategy based on the formation of an RNA-DNA sandwich structure leads to a highly specific electrochemical output. The inkjet-printed nanostructured gold electrodes providing a large surface area enable efficient binding and increase the sensitivity. The inkjet-printed battery-free NFC potentiostat enables rapid measurements and real-time data analysis via a smartphone application, making the platform accessible and portable. With the advantages of speed (5 min), simplicity, sensitivity (low pM range, ∼450% signal gain) and cost-effectiveness, the proposed platform is a promising alternative for point-of-care diagnostics and high-throughput analysis that complements the COVID-19 diagnostic toolkit.
Fil: Rossetti, Marianna. Institut Català de Nanociència I Nanotecnologia; España
Fil: Srisomwat, Chawin. Chulalongkorn University; Tailandia
Fil: Urban, Massimo. Institut Català de Nanociència I Nanotecnologia; España. Universitat Autònoma de Barcelona; España
Fil: Rosati, Giulio. Institut Català de Nanociència I Nanotecnologia; España
Fil: Maroli, Gabriel. Institut Català de Nanociència I Nanotecnologia; España. Universitat Autònoma de Barcelona; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones en Ingeniería Eléctrica "Alfredo Desages". Universidad Nacional del Sur. Departamento de Ingeniería Eléctrica y de Computadoras. Instituto de Investigaciones en Ingeniería Eléctrica "Alfredo Desages"; Argentina
Fil: Yaman Akbay, Hatice Gödze. Institut Català de Nanociència I Nanotecnologia; España
Fil: Chailapakul, Orawon. Chulalongkorn University; Tailandia
Fil: Merkoçi, Arben. Institut Català de Nanociència I Nanotecnologia; España. Institució Catalana de Recerca i Estudis Avancats; España
Materia
BIOSENSORS
ELECTROCHEMISTRY
RNA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/263141
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Unleashing inkjet-printed nanostructured electrodes and battery-free potentiostat for the DNA-based multiplexed detection of SARS-CoV-2 genesRossetti, MariannaSrisomwat, ChawinUrban, MassimoRosati, GiulioMaroli, GabrielYaman Akbay, Hatice GödzeChailapakul, OrawonMerkoçi, ArbenBIOSENSORSELECTROCHEMISTRYRNAhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Following the global COVID-19 pandemic triggered by SARS-CoV-2, the need for rapid, specific and cost-effective point-of-care diagnostic solutions remains paramount. Even though COVID-19 is no longer a public health emergency, the disease still poses a global threat leading to deaths, and it continues to change with the risk of new variants emerging causing a new surge in cases and deaths. Here, we address the urgent need for rapid, cost-effective and point-of-care diagnostic solutions for SARS-CoV-2. We propose a multiplexed DNA-based sensing platform that utilizes inkjet-printed nanostructured gold electrodes and an inkjet-printed battery-free near-field communication (NFC) potentiostat for the simultaneous quantitative detection of two SARS-CoV-2 genes, the ORF1ab and the N gene. The detection strategy based on the formation of an RNA-DNA sandwich structure leads to a highly specific electrochemical output. The inkjet-printed nanostructured gold electrodes providing a large surface area enable efficient binding and increase the sensitivity. The inkjet-printed battery-free NFC potentiostat enables rapid measurements and real-time data analysis via a smartphone application, making the platform accessible and portable. With the advantages of speed (5 min), simplicity, sensitivity (low pM range, ∼450% signal gain) and cost-effectiveness, the proposed platform is a promising alternative for point-of-care diagnostics and high-throughput analysis that complements the COVID-19 diagnostic toolkit.Fil: Rossetti, Marianna. Institut Català de Nanociència I Nanotecnologia; EspañaFil: Srisomwat, Chawin. Chulalongkorn University; TailandiaFil: Urban, Massimo. Institut Català de Nanociència I Nanotecnologia; España. Universitat Autònoma de Barcelona; EspañaFil: Rosati, Giulio. Institut Català de Nanociència I Nanotecnologia; EspañaFil: Maroli, Gabriel. Institut Català de Nanociència I Nanotecnologia; España. Universitat Autònoma de Barcelona; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones en Ingeniería Eléctrica "Alfredo Desages". Universidad Nacional del Sur. Departamento de Ingeniería Eléctrica y de Computadoras. Instituto de Investigaciones en Ingeniería Eléctrica "Alfredo Desages"; ArgentinaFil: Yaman Akbay, Hatice Gödze. Institut Català de Nanociència I Nanotecnologia; EspañaFil: Chailapakul, Orawon. Chulalongkorn University; TailandiaFil: Merkoçi, Arben. Institut Català de Nanociència I Nanotecnologia; España. Institució Catalana de Recerca i Estudis Avancats; EspañaElsevier Advanced Technology2024-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/263141Rossetti, Marianna; Srisomwat, Chawin; Urban, Massimo; Rosati, Giulio; Maroli, Gabriel; et al.; Unleashing inkjet-printed nanostructured electrodes and battery-free potentiostat for the DNA-based multiplexed detection of SARS-CoV-2 genes; Elsevier Advanced Technology; Biosensors & Bioelectronics; 250; 116079; 4-2024; 1-90956-5663CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0956566324000824info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bios.2024.116079info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:23:26Zoai:ri.conicet.gov.ar:11336/263141instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:23:26.866CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Unleashing inkjet-printed nanostructured electrodes and battery-free potentiostat for the DNA-based multiplexed detection of SARS-CoV-2 genes
title Unleashing inkjet-printed nanostructured electrodes and battery-free potentiostat for the DNA-based multiplexed detection of SARS-CoV-2 genes
spellingShingle Unleashing inkjet-printed nanostructured electrodes and battery-free potentiostat for the DNA-based multiplexed detection of SARS-CoV-2 genes
Rossetti, Marianna
BIOSENSORS
ELECTROCHEMISTRY
RNA
title_short Unleashing inkjet-printed nanostructured electrodes and battery-free potentiostat for the DNA-based multiplexed detection of SARS-CoV-2 genes
title_full Unleashing inkjet-printed nanostructured electrodes and battery-free potentiostat for the DNA-based multiplexed detection of SARS-CoV-2 genes
title_fullStr Unleashing inkjet-printed nanostructured electrodes and battery-free potentiostat for the DNA-based multiplexed detection of SARS-CoV-2 genes
title_full_unstemmed Unleashing inkjet-printed nanostructured electrodes and battery-free potentiostat for the DNA-based multiplexed detection of SARS-CoV-2 genes
title_sort Unleashing inkjet-printed nanostructured electrodes and battery-free potentiostat for the DNA-based multiplexed detection of SARS-CoV-2 genes
dc.creator.none.fl_str_mv Rossetti, Marianna
Srisomwat, Chawin
Urban, Massimo
Rosati, Giulio
Maroli, Gabriel
Yaman Akbay, Hatice Gödze
Chailapakul, Orawon
Merkoçi, Arben
author Rossetti, Marianna
author_facet Rossetti, Marianna
Srisomwat, Chawin
Urban, Massimo
Rosati, Giulio
Maroli, Gabriel
Yaman Akbay, Hatice Gödze
Chailapakul, Orawon
Merkoçi, Arben
author_role author
author2 Srisomwat, Chawin
Urban, Massimo
Rosati, Giulio
Maroli, Gabriel
Yaman Akbay, Hatice Gödze
Chailapakul, Orawon
Merkoçi, Arben
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BIOSENSORS
ELECTROCHEMISTRY
RNA
topic BIOSENSORS
ELECTROCHEMISTRY
RNA
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Following the global COVID-19 pandemic triggered by SARS-CoV-2, the need for rapid, specific and cost-effective point-of-care diagnostic solutions remains paramount. Even though COVID-19 is no longer a public health emergency, the disease still poses a global threat leading to deaths, and it continues to change with the risk of new variants emerging causing a new surge in cases and deaths. Here, we address the urgent need for rapid, cost-effective and point-of-care diagnostic solutions for SARS-CoV-2. We propose a multiplexed DNA-based sensing platform that utilizes inkjet-printed nanostructured gold electrodes and an inkjet-printed battery-free near-field communication (NFC) potentiostat for the simultaneous quantitative detection of two SARS-CoV-2 genes, the ORF1ab and the N gene. The detection strategy based on the formation of an RNA-DNA sandwich structure leads to a highly specific electrochemical output. The inkjet-printed nanostructured gold electrodes providing a large surface area enable efficient binding and increase the sensitivity. The inkjet-printed battery-free NFC potentiostat enables rapid measurements and real-time data analysis via a smartphone application, making the platform accessible and portable. With the advantages of speed (5 min), simplicity, sensitivity (low pM range, ∼450% signal gain) and cost-effectiveness, the proposed platform is a promising alternative for point-of-care diagnostics and high-throughput analysis that complements the COVID-19 diagnostic toolkit.
Fil: Rossetti, Marianna. Institut Català de Nanociència I Nanotecnologia; España
Fil: Srisomwat, Chawin. Chulalongkorn University; Tailandia
Fil: Urban, Massimo. Institut Català de Nanociència I Nanotecnologia; España. Universitat Autònoma de Barcelona; España
Fil: Rosati, Giulio. Institut Català de Nanociència I Nanotecnologia; España
Fil: Maroli, Gabriel. Institut Català de Nanociència I Nanotecnologia; España. Universitat Autònoma de Barcelona; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones en Ingeniería Eléctrica "Alfredo Desages". Universidad Nacional del Sur. Departamento de Ingeniería Eléctrica y de Computadoras. Instituto de Investigaciones en Ingeniería Eléctrica "Alfredo Desages"; Argentina
Fil: Yaman Akbay, Hatice Gödze. Institut Català de Nanociència I Nanotecnologia; España
Fil: Chailapakul, Orawon. Chulalongkorn University; Tailandia
Fil: Merkoçi, Arben. Institut Català de Nanociència I Nanotecnologia; España. Institució Catalana de Recerca i Estudis Avancats; España
description Following the global COVID-19 pandemic triggered by SARS-CoV-2, the need for rapid, specific and cost-effective point-of-care diagnostic solutions remains paramount. Even though COVID-19 is no longer a public health emergency, the disease still poses a global threat leading to deaths, and it continues to change with the risk of new variants emerging causing a new surge in cases and deaths. Here, we address the urgent need for rapid, cost-effective and point-of-care diagnostic solutions for SARS-CoV-2. We propose a multiplexed DNA-based sensing platform that utilizes inkjet-printed nanostructured gold electrodes and an inkjet-printed battery-free near-field communication (NFC) potentiostat for the simultaneous quantitative detection of two SARS-CoV-2 genes, the ORF1ab and the N gene. The detection strategy based on the formation of an RNA-DNA sandwich structure leads to a highly specific electrochemical output. The inkjet-printed nanostructured gold electrodes providing a large surface area enable efficient binding and increase the sensitivity. The inkjet-printed battery-free NFC potentiostat enables rapid measurements and real-time data analysis via a smartphone application, making the platform accessible and portable. With the advantages of speed (5 min), simplicity, sensitivity (low pM range, ∼450% signal gain) and cost-effectiveness, the proposed platform is a promising alternative for point-of-care diagnostics and high-throughput analysis that complements the COVID-19 diagnostic toolkit.
publishDate 2024
dc.date.none.fl_str_mv 2024-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/263141
Rossetti, Marianna; Srisomwat, Chawin; Urban, Massimo; Rosati, Giulio; Maroli, Gabriel; et al.; Unleashing inkjet-printed nanostructured electrodes and battery-free potentiostat for the DNA-based multiplexed detection of SARS-CoV-2 genes; Elsevier Advanced Technology; Biosensors & Bioelectronics; 250; 116079; 4-2024; 1-9
0956-5663
CONICET Digital
CONICET
url http://hdl.handle.net/11336/263141
identifier_str_mv Rossetti, Marianna; Srisomwat, Chawin; Urban, Massimo; Rosati, Giulio; Maroli, Gabriel; et al.; Unleashing inkjet-printed nanostructured electrodes and battery-free potentiostat for the DNA-based multiplexed detection of SARS-CoV-2 genes; Elsevier Advanced Technology; Biosensors & Bioelectronics; 250; 116079; 4-2024; 1-9
0956-5663
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0956566324000824
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bios.2024.116079
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Advanced Technology
publisher.none.fl_str_mv Elsevier Advanced Technology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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