Unleashing inkjet-printed nanostructured electrodes and battery-free potentiostat for the DNA-based multiplexed detection of SARS-CoV-2 genes
- Autores
- Rossetti, Marianna; Srisomwat, Chawin; Urban, Massimo; Rosati, Giulio; Maroli, Gabriel; Yaman Akbay, Hatice Gödze; Chailapakul, Orawon; Merkoçi, Arben
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Following the global COVID-19 pandemic triggered by SARS-CoV-2, the need for rapid, specific and cost-effective point-of-care diagnostic solutions remains paramount. Even though COVID-19 is no longer a public health emergency, the disease still poses a global threat leading to deaths, and it continues to change with the risk of new variants emerging causing a new surge in cases and deaths. Here, we address the urgent need for rapid, cost-effective and point-of-care diagnostic solutions for SARS-CoV-2. We propose a multiplexed DNA-based sensing platform that utilizes inkjet-printed nanostructured gold electrodes and an inkjet-printed battery-free near-field communication (NFC) potentiostat for the simultaneous quantitative detection of two SARS-CoV-2 genes, the ORF1ab and the N gene. The detection strategy based on the formation of an RNA-DNA sandwich structure leads to a highly specific electrochemical output. The inkjet-printed nanostructured gold electrodes providing a large surface area enable efficient binding and increase the sensitivity. The inkjet-printed battery-free NFC potentiostat enables rapid measurements and real-time data analysis via a smartphone application, making the platform accessible and portable. With the advantages of speed (5 min), simplicity, sensitivity (low pM range, ∼450% signal gain) and cost-effectiveness, the proposed platform is a promising alternative for point-of-care diagnostics and high-throughput analysis that complements the COVID-19 diagnostic toolkit.
Fil: Rossetti, Marianna. Institut Català de Nanociència I Nanotecnologia; España
Fil: Srisomwat, Chawin. Chulalongkorn University; Tailandia
Fil: Urban, Massimo. Institut Català de Nanociència I Nanotecnologia; España. Universitat Autònoma de Barcelona; España
Fil: Rosati, Giulio. Institut Català de Nanociència I Nanotecnologia; España
Fil: Maroli, Gabriel. Institut Català de Nanociència I Nanotecnologia; España. Universitat Autònoma de Barcelona; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones en Ingeniería Eléctrica "Alfredo Desages". Universidad Nacional del Sur. Departamento de Ingeniería Eléctrica y de Computadoras. Instituto de Investigaciones en Ingeniería Eléctrica "Alfredo Desages"; Argentina
Fil: Yaman Akbay, Hatice Gödze. Institut Català de Nanociència I Nanotecnologia; España
Fil: Chailapakul, Orawon. Chulalongkorn University; Tailandia
Fil: Merkoçi, Arben. Institut Català de Nanociència I Nanotecnologia; España. Institució Catalana de Recerca i Estudis Avancats; España - Materia
-
BIOSENSORS
ELECTROCHEMISTRY
RNA - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/263141
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Unleashing inkjet-printed nanostructured electrodes and battery-free potentiostat for the DNA-based multiplexed detection of SARS-CoV-2 genesRossetti, MariannaSrisomwat, ChawinUrban, MassimoRosati, GiulioMaroli, GabrielYaman Akbay, Hatice GödzeChailapakul, OrawonMerkoçi, ArbenBIOSENSORSELECTROCHEMISTRYRNAhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Following the global COVID-19 pandemic triggered by SARS-CoV-2, the need for rapid, specific and cost-effective point-of-care diagnostic solutions remains paramount. Even though COVID-19 is no longer a public health emergency, the disease still poses a global threat leading to deaths, and it continues to change with the risk of new variants emerging causing a new surge in cases and deaths. Here, we address the urgent need for rapid, cost-effective and point-of-care diagnostic solutions for SARS-CoV-2. We propose a multiplexed DNA-based sensing platform that utilizes inkjet-printed nanostructured gold electrodes and an inkjet-printed battery-free near-field communication (NFC) potentiostat for the simultaneous quantitative detection of two SARS-CoV-2 genes, the ORF1ab and the N gene. The detection strategy based on the formation of an RNA-DNA sandwich structure leads to a highly specific electrochemical output. The inkjet-printed nanostructured gold electrodes providing a large surface area enable efficient binding and increase the sensitivity. The inkjet-printed battery-free NFC potentiostat enables rapid measurements and real-time data analysis via a smartphone application, making the platform accessible and portable. With the advantages of speed (5 min), simplicity, sensitivity (low pM range, ∼450% signal gain) and cost-effectiveness, the proposed platform is a promising alternative for point-of-care diagnostics and high-throughput analysis that complements the COVID-19 diagnostic toolkit.Fil: Rossetti, Marianna. Institut Català de Nanociència I Nanotecnologia; EspañaFil: Srisomwat, Chawin. Chulalongkorn University; TailandiaFil: Urban, Massimo. Institut Català de Nanociència I Nanotecnologia; España. Universitat Autònoma de Barcelona; EspañaFil: Rosati, Giulio. Institut Català de Nanociència I Nanotecnologia; EspañaFil: Maroli, Gabriel. Institut Català de Nanociència I Nanotecnologia; España. Universitat Autònoma de Barcelona; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones en Ingeniería Eléctrica "Alfredo Desages". Universidad Nacional del Sur. Departamento de Ingeniería Eléctrica y de Computadoras. Instituto de Investigaciones en Ingeniería Eléctrica "Alfredo Desages"; ArgentinaFil: Yaman Akbay, Hatice Gödze. Institut Català de Nanociència I Nanotecnologia; EspañaFil: Chailapakul, Orawon. Chulalongkorn University; TailandiaFil: Merkoçi, Arben. Institut Català de Nanociència I Nanotecnologia; España. Institució Catalana de Recerca i Estudis Avancats; EspañaElsevier Advanced Technology2024-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/263141Rossetti, Marianna; Srisomwat, Chawin; Urban, Massimo; Rosati, Giulio; Maroli, Gabriel; et al.; Unleashing inkjet-printed nanostructured electrodes and battery-free potentiostat for the DNA-based multiplexed detection of SARS-CoV-2 genes; Elsevier Advanced Technology; Biosensors & Bioelectronics; 250; 116079; 4-2024; 1-90956-5663CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0956566324000824info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bios.2024.116079info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:26:19Zoai:ri.conicet.gov.ar:11336/263141instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:26:19.824CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Unleashing inkjet-printed nanostructured electrodes and battery-free potentiostat for the DNA-based multiplexed detection of SARS-CoV-2 genes |
title |
Unleashing inkjet-printed nanostructured electrodes and battery-free potentiostat for the DNA-based multiplexed detection of SARS-CoV-2 genes |
spellingShingle |
Unleashing inkjet-printed nanostructured electrodes and battery-free potentiostat for the DNA-based multiplexed detection of SARS-CoV-2 genes Rossetti, Marianna BIOSENSORS ELECTROCHEMISTRY RNA |
title_short |
Unleashing inkjet-printed nanostructured electrodes and battery-free potentiostat for the DNA-based multiplexed detection of SARS-CoV-2 genes |
title_full |
Unleashing inkjet-printed nanostructured electrodes and battery-free potentiostat for the DNA-based multiplexed detection of SARS-CoV-2 genes |
title_fullStr |
Unleashing inkjet-printed nanostructured electrodes and battery-free potentiostat for the DNA-based multiplexed detection of SARS-CoV-2 genes |
title_full_unstemmed |
Unleashing inkjet-printed nanostructured electrodes and battery-free potentiostat for the DNA-based multiplexed detection of SARS-CoV-2 genes |
title_sort |
Unleashing inkjet-printed nanostructured electrodes and battery-free potentiostat for the DNA-based multiplexed detection of SARS-CoV-2 genes |
dc.creator.none.fl_str_mv |
Rossetti, Marianna Srisomwat, Chawin Urban, Massimo Rosati, Giulio Maroli, Gabriel Yaman Akbay, Hatice Gödze Chailapakul, Orawon Merkoçi, Arben |
author |
Rossetti, Marianna |
author_facet |
Rossetti, Marianna Srisomwat, Chawin Urban, Massimo Rosati, Giulio Maroli, Gabriel Yaman Akbay, Hatice Gödze Chailapakul, Orawon Merkoçi, Arben |
author_role |
author |
author2 |
Srisomwat, Chawin Urban, Massimo Rosati, Giulio Maroli, Gabriel Yaman Akbay, Hatice Gödze Chailapakul, Orawon Merkoçi, Arben |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
BIOSENSORS ELECTROCHEMISTRY RNA |
topic |
BIOSENSORS ELECTROCHEMISTRY RNA |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.4 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Following the global COVID-19 pandemic triggered by SARS-CoV-2, the need for rapid, specific and cost-effective point-of-care diagnostic solutions remains paramount. Even though COVID-19 is no longer a public health emergency, the disease still poses a global threat leading to deaths, and it continues to change with the risk of new variants emerging causing a new surge in cases and deaths. Here, we address the urgent need for rapid, cost-effective and point-of-care diagnostic solutions for SARS-CoV-2. We propose a multiplexed DNA-based sensing platform that utilizes inkjet-printed nanostructured gold electrodes and an inkjet-printed battery-free near-field communication (NFC) potentiostat for the simultaneous quantitative detection of two SARS-CoV-2 genes, the ORF1ab and the N gene. The detection strategy based on the formation of an RNA-DNA sandwich structure leads to a highly specific electrochemical output. The inkjet-printed nanostructured gold electrodes providing a large surface area enable efficient binding and increase the sensitivity. The inkjet-printed battery-free NFC potentiostat enables rapid measurements and real-time data analysis via a smartphone application, making the platform accessible and portable. With the advantages of speed (5 min), simplicity, sensitivity (low pM range, ∼450% signal gain) and cost-effectiveness, the proposed platform is a promising alternative for point-of-care diagnostics and high-throughput analysis that complements the COVID-19 diagnostic toolkit. Fil: Rossetti, Marianna. Institut Català de Nanociència I Nanotecnologia; España Fil: Srisomwat, Chawin. Chulalongkorn University; Tailandia Fil: Urban, Massimo. Institut Català de Nanociència I Nanotecnologia; España. Universitat Autònoma de Barcelona; España Fil: Rosati, Giulio. Institut Català de Nanociència I Nanotecnologia; España Fil: Maroli, Gabriel. Institut Català de Nanociència I Nanotecnologia; España. Universitat Autònoma de Barcelona; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones en Ingeniería Eléctrica "Alfredo Desages". Universidad Nacional del Sur. Departamento de Ingeniería Eléctrica y de Computadoras. Instituto de Investigaciones en Ingeniería Eléctrica "Alfredo Desages"; Argentina Fil: Yaman Akbay, Hatice Gödze. Institut Català de Nanociència I Nanotecnologia; España Fil: Chailapakul, Orawon. Chulalongkorn University; Tailandia Fil: Merkoçi, Arben. Institut Català de Nanociència I Nanotecnologia; España. Institució Catalana de Recerca i Estudis Avancats; España |
description |
Following the global COVID-19 pandemic triggered by SARS-CoV-2, the need for rapid, specific and cost-effective point-of-care diagnostic solutions remains paramount. Even though COVID-19 is no longer a public health emergency, the disease still poses a global threat leading to deaths, and it continues to change with the risk of new variants emerging causing a new surge in cases and deaths. Here, we address the urgent need for rapid, cost-effective and point-of-care diagnostic solutions for SARS-CoV-2. We propose a multiplexed DNA-based sensing platform that utilizes inkjet-printed nanostructured gold electrodes and an inkjet-printed battery-free near-field communication (NFC) potentiostat for the simultaneous quantitative detection of two SARS-CoV-2 genes, the ORF1ab and the N gene. The detection strategy based on the formation of an RNA-DNA sandwich structure leads to a highly specific electrochemical output. The inkjet-printed nanostructured gold electrodes providing a large surface area enable efficient binding and increase the sensitivity. The inkjet-printed battery-free NFC potentiostat enables rapid measurements and real-time data analysis via a smartphone application, making the platform accessible and portable. With the advantages of speed (5 min), simplicity, sensitivity (low pM range, ∼450% signal gain) and cost-effectiveness, the proposed platform is a promising alternative for point-of-care diagnostics and high-throughput analysis that complements the COVID-19 diagnostic toolkit. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/263141 Rossetti, Marianna; Srisomwat, Chawin; Urban, Massimo; Rosati, Giulio; Maroli, Gabriel; et al.; Unleashing inkjet-printed nanostructured electrodes and battery-free potentiostat for the DNA-based multiplexed detection of SARS-CoV-2 genes; Elsevier Advanced Technology; Biosensors & Bioelectronics; 250; 116079; 4-2024; 1-9 0956-5663 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/263141 |
identifier_str_mv |
Rossetti, Marianna; Srisomwat, Chawin; Urban, Massimo; Rosati, Giulio; Maroli, Gabriel; et al.; Unleashing inkjet-printed nanostructured electrodes and battery-free potentiostat for the DNA-based multiplexed detection of SARS-CoV-2 genes; Elsevier Advanced Technology; Biosensors & Bioelectronics; 250; 116079; 4-2024; 1-9 0956-5663 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0956566324000824 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bios.2024.116079 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Advanced Technology |
publisher.none.fl_str_mv |
Elsevier Advanced Technology |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614264297357312 |
score |
13.070432 |