Developing an elegant and integrated electrochemical-theoretical approach for detection of DNA damage induced by 4-nonylphenol

Autores
Ghanbari, Kazhal; Roshani, Mahmoud; Goicoechea, Hector Casimiro; Jalalvand, Ali R.
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this work, a novel biosensor was fabricated for detection of DNA damage induced by 4-nonylphenol (NP) and also determination of NP. To achieve this goal, a glassy carbon electrode (GCE) was modified with chitosan (Chit), gold nanoparticles (Au NPs) and DNA-multiwalled carbon nanotubes (DNA-MWCNTs). Then, the DNA-MWCNTs/Au NPs/Chit/GCE was incubated with methylene blue (MB) to obtain MB-DNA-MWCNTs/Au NPs/Chit/GCE in which MB was used as the redox indicator. The modifications applied to the GCE were characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopic (EDS) and theoretical evidence. MB is a derivative of anthraquinone which can intercalate into double helix structure of DNA. By treating MB-DNA-MWCNTs/Au NPs/Chit/GCE with NP, a higher Rct was observed because the insertion of the NP may result in a more negative charge environment on the DNA surface which hinders accessibility of [Fe(CN)6]3-/4- anion to the electrode surface. Change in the EIS response of the biosensor in the presence of NP was used to develop a novel system for monitoring the DNA damage induced by NP. The EIS technique was also used to develop a sensitive electroanalytical method for determination of NP.
Fil: Ghanbari, Kazhal. Ilam University; Irán
Fil: Roshani, Mahmoud. Ilam University; Irán
Fil: Goicoechea, Hector Casimiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina
Fil: Jalalvand, Ali R.. Kermanshah University of Medical Sciences; Irán
Materia
4-NONYLPHENOL
ANALYTICAL CHEMISTRY
DNA DAMAGE
ELECTROCHEMICAL BIOSENSOR
ELECTROCHEMISTRY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/149606
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/149606
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Developing an elegant and integrated electrochemical-theoretical approach for detection of DNA damage induced by 4-nonylphenolGhanbari, KazhalRoshani, MahmoudGoicoechea, Hector CasimiroJalalvand, Ali R.4-NONYLPHENOLANALYTICAL CHEMISTRYDNA DAMAGEELECTROCHEMICAL BIOSENSORELECTROCHEMISTRYhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In this work, a novel biosensor was fabricated for detection of DNA damage induced by 4-nonylphenol (NP) and also determination of NP. To achieve this goal, a glassy carbon electrode (GCE) was modified with chitosan (Chit), gold nanoparticles (Au NPs) and DNA-multiwalled carbon nanotubes (DNA-MWCNTs). Then, the DNA-MWCNTs/Au NPs/Chit/GCE was incubated with methylene blue (MB) to obtain MB-DNA-MWCNTs/Au NPs/Chit/GCE in which MB was used as the redox indicator. The modifications applied to the GCE were characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopic (EDS) and theoretical evidence. MB is a derivative of anthraquinone which can intercalate into double helix structure of DNA. By treating MB-DNA-MWCNTs/Au NPs/Chit/GCE with NP, a higher Rct was observed because the insertion of the NP may result in a more negative charge environment on the DNA surface which hinders accessibility of [Fe(CN)6]3-/4- anion to the electrode surface. Change in the EIS response of the biosensor in the presence of NP was used to develop a novel system for monitoring the DNA damage induced by NP. The EIS technique was also used to develop a sensitive electroanalytical method for determination of NP.Fil: Ghanbari, Kazhal. Ilam University; IránFil: Roshani, Mahmoud. Ilam University; IránFil: Goicoechea, Hector Casimiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Analítico y Quimiometría; ArgentinaFil: Jalalvand, Ali R.. Kermanshah University of Medical Sciences; IránElsevier2019-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/149606Ghanbari, Kazhal; Roshani, Mahmoud; Goicoechea, Hector Casimiro; Jalalvand, Ali R.; Developing an elegant and integrated electrochemical-theoretical approach for detection of DNA damage induced by 4-nonylphenol; Elsevier; Heliyon; 5; 10; 10-2019; 1-92405-8440CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.heliyon.2019.e02755info:eu-repo/semantics/altIdentifier/url/https://www.cell.com/heliyon/fulltext/S2405-8440(19)36415-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:23:27Zoai:ri.conicet.gov.ar:11336/149606instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:23:27.864CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Developing an elegant and integrated electrochemical-theoretical approach for detection of DNA damage induced by 4-nonylphenol
title Developing an elegant and integrated electrochemical-theoretical approach for detection of DNA damage induced by 4-nonylphenol
spellingShingle Developing an elegant and integrated electrochemical-theoretical approach for detection of DNA damage induced by 4-nonylphenol
Ghanbari, Kazhal
4-NONYLPHENOL
ANALYTICAL CHEMISTRY
DNA DAMAGE
ELECTROCHEMICAL BIOSENSOR
ELECTROCHEMISTRY
title_short Developing an elegant and integrated electrochemical-theoretical approach for detection of DNA damage induced by 4-nonylphenol
title_full Developing an elegant and integrated electrochemical-theoretical approach for detection of DNA damage induced by 4-nonylphenol
title_fullStr Developing an elegant and integrated electrochemical-theoretical approach for detection of DNA damage induced by 4-nonylphenol
title_full_unstemmed Developing an elegant and integrated electrochemical-theoretical approach for detection of DNA damage induced by 4-nonylphenol
title_sort Developing an elegant and integrated electrochemical-theoretical approach for detection of DNA damage induced by 4-nonylphenol
dc.creator.none.fl_str_mv Ghanbari, Kazhal
Roshani, Mahmoud
Goicoechea, Hector Casimiro
Jalalvand, Ali R.
author Ghanbari, Kazhal
author_facet Ghanbari, Kazhal
Roshani, Mahmoud
Goicoechea, Hector Casimiro
Jalalvand, Ali R.
author_role author
author2 Roshani, Mahmoud
Goicoechea, Hector Casimiro
Jalalvand, Ali R.
author2_role author
author
author
dc.subject.none.fl_str_mv 4-NONYLPHENOL
ANALYTICAL CHEMISTRY
DNA DAMAGE
ELECTROCHEMICAL BIOSENSOR
ELECTROCHEMISTRY
topic 4-NONYLPHENOL
ANALYTICAL CHEMISTRY
DNA DAMAGE
ELECTROCHEMICAL BIOSENSOR
ELECTROCHEMISTRY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this work, a novel biosensor was fabricated for detection of DNA damage induced by 4-nonylphenol (NP) and also determination of NP. To achieve this goal, a glassy carbon electrode (GCE) was modified with chitosan (Chit), gold nanoparticles (Au NPs) and DNA-multiwalled carbon nanotubes (DNA-MWCNTs). Then, the DNA-MWCNTs/Au NPs/Chit/GCE was incubated with methylene blue (MB) to obtain MB-DNA-MWCNTs/Au NPs/Chit/GCE in which MB was used as the redox indicator. The modifications applied to the GCE were characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopic (EDS) and theoretical evidence. MB is a derivative of anthraquinone which can intercalate into double helix structure of DNA. By treating MB-DNA-MWCNTs/Au NPs/Chit/GCE with NP, a higher Rct was observed because the insertion of the NP may result in a more negative charge environment on the DNA surface which hinders accessibility of [Fe(CN)6]3-/4- anion to the electrode surface. Change in the EIS response of the biosensor in the presence of NP was used to develop a novel system for monitoring the DNA damage induced by NP. The EIS technique was also used to develop a sensitive electroanalytical method for determination of NP.
Fil: Ghanbari, Kazhal. Ilam University; Irán
Fil: Roshani, Mahmoud. Ilam University; Irán
Fil: Goicoechea, Hector Casimiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Desarrollo Analítico y Quimiometría; Argentina
Fil: Jalalvand, Ali R.. Kermanshah University of Medical Sciences; Irán
description In this work, a novel biosensor was fabricated for detection of DNA damage induced by 4-nonylphenol (NP) and also determination of NP. To achieve this goal, a glassy carbon electrode (GCE) was modified with chitosan (Chit), gold nanoparticles (Au NPs) and DNA-multiwalled carbon nanotubes (DNA-MWCNTs). Then, the DNA-MWCNTs/Au NPs/Chit/GCE was incubated with methylene blue (MB) to obtain MB-DNA-MWCNTs/Au NPs/Chit/GCE in which MB was used as the redox indicator. The modifications applied to the GCE were characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopic (EDS) and theoretical evidence. MB is a derivative of anthraquinone which can intercalate into double helix structure of DNA. By treating MB-DNA-MWCNTs/Au NPs/Chit/GCE with NP, a higher Rct was observed because the insertion of the NP may result in a more negative charge environment on the DNA surface which hinders accessibility of [Fe(CN)6]3-/4- anion to the electrode surface. Change in the EIS response of the biosensor in the presence of NP was used to develop a novel system for monitoring the DNA damage induced by NP. The EIS technique was also used to develop a sensitive electroanalytical method for determination of NP.
publishDate 2019
dc.date.none.fl_str_mv 2019-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/149606
Ghanbari, Kazhal; Roshani, Mahmoud; Goicoechea, Hector Casimiro; Jalalvand, Ali R.; Developing an elegant and integrated electrochemical-theoretical approach for detection of DNA damage induced by 4-nonylphenol; Elsevier; Heliyon; 5; 10; 10-2019; 1-9
2405-8440
CONICET Digital
CONICET
url http://hdl.handle.net/11336/149606
identifier_str_mv Ghanbari, Kazhal; Roshani, Mahmoud; Goicoechea, Hector Casimiro; Jalalvand, Ali R.; Developing an elegant and integrated electrochemical-theoretical approach for detection of DNA damage induced by 4-nonylphenol; Elsevier; Heliyon; 5; 10; 10-2019; 1-9
2405-8440
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.heliyon.2019.e02755
info:eu-repo/semantics/altIdentifier/url/https://www.cell.com/heliyon/fulltext/S2405-8440(19)36415-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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