Stinging caterpillars from the genera Podalia , Leucanella and Lonomia in Misiones, Argentina: A preliminary comparative approach to understand their toxicity

Autores
Quintana, María Agustina; Mozer Sciani, Juliana; Vatti Auada, Aline Vivian; Martínez, María Mercedes; Sanchez, Matias Nicolas; Santoro, Marcelo Larami; Fan, Hui Wen; Peichoto, María Elisa
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Dermal contact with Lepidoptera specimens at their larval stage (caterpillar) may cause systemic and/or local envenomation. There are multiple venomous species of them in Argentina, but their overall venom composition is poorly known. Lately, several cases of envenomation have been reported in the Misiones province, Northeastern Argentina. Thus, this work aimed to compare the protein composition, and the enzymatic properties of bristle extracts from caterpillars belonging to the families Megalopygidae (Podalia ca. fuscescens) and Saturniidae (Leucanella memusae and Lonomia obliqua) - the most common causative agents of accidents in Misiones -, and additionally to test their cross-reactivity with the L. obliqua antivenom produced in Brazil. Saturniidae venoms exhibited striking similarity in both their electrophoretic protein profile, and antigenic cross-reactivity. All venoms degraded azocasein ? with the highest proteolytic activity observed in the P. ca. fuscescens bristle extract ?, and hyaluronic acid, but the latter at low levels. Lonomia obliqua venom exhibited the highest level of phospholipase A2 activity. Bristle extracts from P. ca. fuscescens and L. obliqua both degraded human fibrin(ogen) and shortened the clotting time triggered by calcium, while L. memusae venom inhibited plasma coagulation. Proteins related to the coagulation disturbance were identified by mass spectrometry in all samples. Altogether, our findings show for the first time a comparative biotoxinological analysis of three genera of caterpillars with medical relevance. Moreover, this study provides relevant information about the pathophysiological mechanisms whereby these caterpillar bristle extracts can induce toxicity on human beings, and gives insight into future directions for research on them.
Fil: Quintana, María Agustina. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina
Fil: Mozer Sciani, Juliana. Governo Do Estado de Sao Paulo. Secretaria Da Saude. Instituto Butantan; Brasil
Fil: Vatti Auada, Aline Vivian. Governo Do Estado de Sao Paulo. Secretaria Da Saude. Instituto Butantan; Brasil
Fil: Martínez, María Mercedes. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina
Fil: Sanchez, Matias Nicolas. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Santoro, Marcelo Larami. Governo Do Estado de Sao Paulo. Secretaria Da Saude. Instituto Butantan; Brasil
Fil: Fan, Hui Wen. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina
Fil: Peichoto, María Elisa. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Caterpillars
Coagulation
Hemostasis
Lonomia Antivenom
Megalopygidae
Saturniidae
South America
Spines
Venoms
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41213
Acceder
id CONICETDig_56beb5e4ef17367ec134639fbcd349e7
oai_identifier_str oai:ri.conicet.gov.ar:11336/41213
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Stinging caterpillars from the genera Podalia , Leucanella and Lonomia in Misiones, Argentina: A preliminary comparative approach to understand their toxicityQuintana, María AgustinaMozer Sciani, JulianaVatti Auada, Aline VivianMartínez, María MercedesSanchez, Matias NicolasSantoro, Marcelo LaramiFan, Hui WenPeichoto, María ElisaCaterpillarsCoagulationHemostasisLonomia AntivenomMegalopygidaeSaturniidaeSouth AmericaSpinesVenomshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Dermal contact with Lepidoptera specimens at their larval stage (caterpillar) may cause systemic and/or local envenomation. There are multiple venomous species of them in Argentina, but their overall venom composition is poorly known. Lately, several cases of envenomation have been reported in the Misiones province, Northeastern Argentina. Thus, this work aimed to compare the protein composition, and the enzymatic properties of bristle extracts from caterpillars belonging to the families Megalopygidae (Podalia ca. fuscescens) and Saturniidae (Leucanella memusae and Lonomia obliqua) - the most common causative agents of accidents in Misiones -, and additionally to test their cross-reactivity with the L. obliqua antivenom produced in Brazil. Saturniidae venoms exhibited striking similarity in both their electrophoretic protein profile, and antigenic cross-reactivity. All venoms degraded azocasein ? with the highest proteolytic activity observed in the P. ca. fuscescens bristle extract ?, and hyaluronic acid, but the latter at low levels. Lonomia obliqua venom exhibited the highest level of phospholipase A2 activity. Bristle extracts from P. ca. fuscescens and L. obliqua both degraded human fibrin(ogen) and shortened the clotting time triggered by calcium, while L. memusae venom inhibited plasma coagulation. Proteins related to the coagulation disturbance were identified by mass spectrometry in all samples. Altogether, our findings show for the first time a comparative biotoxinological analysis of three genera of caterpillars with medical relevance. Moreover, this study provides relevant information about the pathophysiological mechanisms whereby these caterpillar bristle extracts can induce toxicity on human beings, and gives insight into future directions for research on them.Fil: Quintana, María Agustina. Ministerio de Salud. Instituto Nacional de Medicina Tropical; ArgentinaFil: Mozer Sciani, Juliana. Governo Do Estado de Sao Paulo. Secretaria Da Saude. Instituto Butantan; BrasilFil: Vatti Auada, Aline Vivian. Governo Do Estado de Sao Paulo. Secretaria Da Saude. Instituto Butantan; BrasilFil: Martínez, María Mercedes. Ministerio de Salud. Instituto Nacional de Medicina Tropical; ArgentinaFil: Sanchez, Matias Nicolas. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Santoro, Marcelo Larami. Governo Do Estado de Sao Paulo. Secretaria Da Saude. Instituto Butantan; BrasilFil: Fan, Hui Wen. Ministerio de Salud. Instituto Nacional de Medicina Tropical; ArgentinaFil: Peichoto, María Elisa. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science Inc2017-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41213Quintana, María Agustina; Mozer Sciani, Juliana; Vatti Auada, Aline Vivian; Martínez, María Mercedes; Sanchez, Matias Nicolas; et al.; Stinging caterpillars from the genera Podalia , Leucanella and Lonomia in Misiones, Argentina: A preliminary comparative approach to understand their toxicity; Elsevier Science Inc; Comparative Biochemistry And Physiology. Toxicology & Pharmacology; 202; 11-2017; 55-621532-04561532-0456CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpc.2017.07.007info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1532045617301382info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:41:10Zoai:ri.conicet.gov.ar:11336/41213instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:41:10.633CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Stinging caterpillars from the genera Podalia , Leucanella and Lonomia in Misiones, Argentina: A preliminary comparative approach to understand their toxicity
title Stinging caterpillars from the genera Podalia , Leucanella and Lonomia in Misiones, Argentina: A preliminary comparative approach to understand their toxicity
spellingShingle Stinging caterpillars from the genera Podalia , Leucanella and Lonomia in Misiones, Argentina: A preliminary comparative approach to understand their toxicity
Quintana, María Agustina
Caterpillars
Coagulation
Hemostasis
Lonomia Antivenom
Megalopygidae
Saturniidae
South America
Spines
Venoms
title_short Stinging caterpillars from the genera Podalia , Leucanella and Lonomia in Misiones, Argentina: A preliminary comparative approach to understand their toxicity
title_full Stinging caterpillars from the genera Podalia , Leucanella and Lonomia in Misiones, Argentina: A preliminary comparative approach to understand their toxicity
title_fullStr Stinging caterpillars from the genera Podalia , Leucanella and Lonomia in Misiones, Argentina: A preliminary comparative approach to understand their toxicity
title_full_unstemmed Stinging caterpillars from the genera Podalia , Leucanella and Lonomia in Misiones, Argentina: A preliminary comparative approach to understand their toxicity
title_sort Stinging caterpillars from the genera Podalia , Leucanella and Lonomia in Misiones, Argentina: A preliminary comparative approach to understand their toxicity
dc.creator.none.fl_str_mv Quintana, María Agustina
Mozer Sciani, Juliana
Vatti Auada, Aline Vivian
Martínez, María Mercedes
Sanchez, Matias Nicolas
Santoro, Marcelo Larami
Fan, Hui Wen
Peichoto, María Elisa
author Quintana, María Agustina
author_facet Quintana, María Agustina
Mozer Sciani, Juliana
Vatti Auada, Aline Vivian
Martínez, María Mercedes
Sanchez, Matias Nicolas
Santoro, Marcelo Larami
Fan, Hui Wen
Peichoto, María Elisa
author_role author
author2 Mozer Sciani, Juliana
Vatti Auada, Aline Vivian
Martínez, María Mercedes
Sanchez, Matias Nicolas
Santoro, Marcelo Larami
Fan, Hui Wen
Peichoto, María Elisa
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Caterpillars
Coagulation
Hemostasis
Lonomia Antivenom
Megalopygidae
Saturniidae
South America
Spines
Venoms
topic Caterpillars
Coagulation
Hemostasis
Lonomia Antivenom
Megalopygidae
Saturniidae
South America
Spines
Venoms
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Dermal contact with Lepidoptera specimens at their larval stage (caterpillar) may cause systemic and/or local envenomation. There are multiple venomous species of them in Argentina, but their overall venom composition is poorly known. Lately, several cases of envenomation have been reported in the Misiones province, Northeastern Argentina. Thus, this work aimed to compare the protein composition, and the enzymatic properties of bristle extracts from caterpillars belonging to the families Megalopygidae (Podalia ca. fuscescens) and Saturniidae (Leucanella memusae and Lonomia obliqua) - the most common causative agents of accidents in Misiones -, and additionally to test their cross-reactivity with the L. obliqua antivenom produced in Brazil. Saturniidae venoms exhibited striking similarity in both their electrophoretic protein profile, and antigenic cross-reactivity. All venoms degraded azocasein ? with the highest proteolytic activity observed in the P. ca. fuscescens bristle extract ?, and hyaluronic acid, but the latter at low levels. Lonomia obliqua venom exhibited the highest level of phospholipase A2 activity. Bristle extracts from P. ca. fuscescens and L. obliqua both degraded human fibrin(ogen) and shortened the clotting time triggered by calcium, while L. memusae venom inhibited plasma coagulation. Proteins related to the coagulation disturbance were identified by mass spectrometry in all samples. Altogether, our findings show for the first time a comparative biotoxinological analysis of three genera of caterpillars with medical relevance. Moreover, this study provides relevant information about the pathophysiological mechanisms whereby these caterpillar bristle extracts can induce toxicity on human beings, and gives insight into future directions for research on them.
Fil: Quintana, María Agustina. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina
Fil: Mozer Sciani, Juliana. Governo Do Estado de Sao Paulo. Secretaria Da Saude. Instituto Butantan; Brasil
Fil: Vatti Auada, Aline Vivian. Governo Do Estado de Sao Paulo. Secretaria Da Saude. Instituto Butantan; Brasil
Fil: Martínez, María Mercedes. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina
Fil: Sanchez, Matias Nicolas. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Santoro, Marcelo Larami. Governo Do Estado de Sao Paulo. Secretaria Da Saude. Instituto Butantan; Brasil
Fil: Fan, Hui Wen. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina
Fil: Peichoto, María Elisa. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Dermal contact with Lepidoptera specimens at their larval stage (caterpillar) may cause systemic and/or local envenomation. There are multiple venomous species of them in Argentina, but their overall venom composition is poorly known. Lately, several cases of envenomation have been reported in the Misiones province, Northeastern Argentina. Thus, this work aimed to compare the protein composition, and the enzymatic properties of bristle extracts from caterpillars belonging to the families Megalopygidae (Podalia ca. fuscescens) and Saturniidae (Leucanella memusae and Lonomia obliqua) - the most common causative agents of accidents in Misiones -, and additionally to test their cross-reactivity with the L. obliqua antivenom produced in Brazil. Saturniidae venoms exhibited striking similarity in both their electrophoretic protein profile, and antigenic cross-reactivity. All venoms degraded azocasein ? with the highest proteolytic activity observed in the P. ca. fuscescens bristle extract ?, and hyaluronic acid, but the latter at low levels. Lonomia obliqua venom exhibited the highest level of phospholipase A2 activity. Bristle extracts from P. ca. fuscescens and L. obliqua both degraded human fibrin(ogen) and shortened the clotting time triggered by calcium, while L. memusae venom inhibited plasma coagulation. Proteins related to the coagulation disturbance were identified by mass spectrometry in all samples. Altogether, our findings show for the first time a comparative biotoxinological analysis of three genera of caterpillars with medical relevance. Moreover, this study provides relevant information about the pathophysiological mechanisms whereby these caterpillar bristle extracts can induce toxicity on human beings, and gives insight into future directions for research on them.
publishDate 2017
dc.date.none.fl_str_mv 2017-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41213
Quintana, María Agustina; Mozer Sciani, Juliana; Vatti Auada, Aline Vivian; Martínez, María Mercedes; Sanchez, Matias Nicolas; et al.; Stinging caterpillars from the genera Podalia , Leucanella and Lonomia in Misiones, Argentina: A preliminary comparative approach to understand their toxicity; Elsevier Science Inc; Comparative Biochemistry And Physiology. Toxicology & Pharmacology; 202; 11-2017; 55-62
1532-0456
1532-0456
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41213
identifier_str_mv Quintana, María Agustina; Mozer Sciani, Juliana; Vatti Auada, Aline Vivian; Martínez, María Mercedes; Sanchez, Matias Nicolas; et al.; Stinging caterpillars from the genera Podalia , Leucanella and Lonomia in Misiones, Argentina: A preliminary comparative approach to understand their toxicity; Elsevier Science Inc; Comparative Biochemistry And Physiology. Toxicology & Pharmacology; 202; 11-2017; 55-62
1532-0456
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpc.2017.07.007
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1532045617301382
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613301279916032
score 13.070432

Publicaciones similares