In Vitro Macrophage Assay Predicts the In Vivo Anti-inflammatory Potential of Exosomes from Human Mesenchymal Stromal Cells

Autores
Pacienza, Natalia Alejandra; Lee, Ryang Hwa; Bae, Eun-Hye; Kim, Dong-ki; Liu, Qisong; Prockop, Darwin J.; Yannarelli, Gustavo Gabriel
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Extracellular vesicles (EVs) play key roles in cell biology and may provide new clinical diagnostics and therapies. However, it has proven difficult to develop protocols for their purification and characterization. One of the major barriers in the field has been a lack of convenient assays for their bioactivity. Developing assays has not been a trivial matter, because of the heterogeneity of EVs, the multiple activities they demonstrate, and the uncertainty about their modes of action. Therefore, it is likely that multiple assays for their activities are needed. One important assay will be for the anti-inflammatory activity observed in mice after administration of the small EVs commonly referred to as exosomes. We developed an assay for the anti-inflammatory activity of exosomes with a line of mouse macrophages. The assay makes it possible to rank different preparations of exosomes by their anti-inflammatory activity, and their ranking predicts their efficacy in suppressing LPS-stimulated inflammation in mice. The assay is convenient for comparing multiple samples and, therefore, should be useful in developing protocols for the purification and characterization of anti-inflammatory exosomes.
Fil: Pacienza, Natalia Alejandra. Texas A&M University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina
Fil: Lee, Ryang Hwa. Texas A&M University; Estados Unidos
Fil: Bae, Eun-Hye. Texas A&M University; Estados Unidos
Fil: Kim, Dong-ki. Texas A&M University; Estados Unidos
Fil: Liu, Qisong. Texas A&M University; Estados Unidos
Fil: Prockop, Darwin J.. Texas A&M University; Estados Unidos
Fil: Yannarelli, Gustavo Gabriel. Texas A&M University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina
Materia
EXOSOMES
MESENCHYMAL STROMAL CELLS
BIOACTIVITY
INFLAMMATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/121379
Acceder
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spelling In Vitro Macrophage Assay Predicts the In Vivo Anti-inflammatory Potential of Exosomes from Human Mesenchymal Stromal CellsPacienza, Natalia AlejandraLee, Ryang HwaBae, Eun-HyeKim, Dong-kiLiu, QisongProckop, Darwin J.Yannarelli, Gustavo GabrielEXOSOMESMESENCHYMAL STROMAL CELLSBIOACTIVITYINFLAMMATIONhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Extracellular vesicles (EVs) play key roles in cell biology and may provide new clinical diagnostics and therapies. However, it has proven difficult to develop protocols for their purification and characterization. One of the major barriers in the field has been a lack of convenient assays for their bioactivity. Developing assays has not been a trivial matter, because of the heterogeneity of EVs, the multiple activities they demonstrate, and the uncertainty about their modes of action. Therefore, it is likely that multiple assays for their activities are needed. One important assay will be for the anti-inflammatory activity observed in mice after administration of the small EVs commonly referred to as exosomes. We developed an assay for the anti-inflammatory activity of exosomes with a line of mouse macrophages. The assay makes it possible to rank different preparations of exosomes by their anti-inflammatory activity, and their ranking predicts their efficacy in suppressing LPS-stimulated inflammation in mice. The assay is convenient for comparing multiple samples and, therefore, should be useful in developing protocols for the purification and characterization of anti-inflammatory exosomes.Fil: Pacienza, Natalia Alejandra. Texas A&M University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; ArgentinaFil: Lee, Ryang Hwa. Texas A&M University; Estados UnidosFil: Bae, Eun-Hye. Texas A&M University; Estados UnidosFil: Kim, Dong-ki. Texas A&M University; Estados UnidosFil: Liu, Qisong. Texas A&M University; Estados UnidosFil: Prockop, Darwin J.. Texas A&M University; Estados UnidosFil: Yannarelli, Gustavo Gabriel. Texas A&M University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; ArgentinaCell Press2019-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/121379Pacienza, Natalia Alejandra; Lee, Ryang Hwa; Bae, Eun-Hye; Kim, Dong-ki; Liu, Qisong; et al.; In Vitro Macrophage Assay Predicts the In Vivo Anti-inflammatory Potential of Exosomes from Human Mesenchymal Stromal Cells; Cell Press; Molecular Therapy:Methods and Clinical Development; 13; 6-2019; 67-762329-0501CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.omtm.2018.12.003info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:14:32Zoai:ri.conicet.gov.ar:11336/121379instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:14:32.972CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In Vitro Macrophage Assay Predicts the In Vivo Anti-inflammatory Potential of Exosomes from Human Mesenchymal Stromal Cells
title In Vitro Macrophage Assay Predicts the In Vivo Anti-inflammatory Potential of Exosomes from Human Mesenchymal Stromal Cells
spellingShingle In Vitro Macrophage Assay Predicts the In Vivo Anti-inflammatory Potential of Exosomes from Human Mesenchymal Stromal Cells
Pacienza, Natalia Alejandra
EXOSOMES
MESENCHYMAL STROMAL CELLS
BIOACTIVITY
INFLAMMATION
title_short In Vitro Macrophage Assay Predicts the In Vivo Anti-inflammatory Potential of Exosomes from Human Mesenchymal Stromal Cells
title_full In Vitro Macrophage Assay Predicts the In Vivo Anti-inflammatory Potential of Exosomes from Human Mesenchymal Stromal Cells
title_fullStr In Vitro Macrophage Assay Predicts the In Vivo Anti-inflammatory Potential of Exosomes from Human Mesenchymal Stromal Cells
title_full_unstemmed In Vitro Macrophage Assay Predicts the In Vivo Anti-inflammatory Potential of Exosomes from Human Mesenchymal Stromal Cells
title_sort In Vitro Macrophage Assay Predicts the In Vivo Anti-inflammatory Potential of Exosomes from Human Mesenchymal Stromal Cells
dc.creator.none.fl_str_mv Pacienza, Natalia Alejandra
Lee, Ryang Hwa
Bae, Eun-Hye
Kim, Dong-ki
Liu, Qisong
Prockop, Darwin J.
Yannarelli, Gustavo Gabriel
author Pacienza, Natalia Alejandra
author_facet Pacienza, Natalia Alejandra
Lee, Ryang Hwa
Bae, Eun-Hye
Kim, Dong-ki
Liu, Qisong
Prockop, Darwin J.
Yannarelli, Gustavo Gabriel
author_role author
author2 Lee, Ryang Hwa
Bae, Eun-Hye
Kim, Dong-ki
Liu, Qisong
Prockop, Darwin J.
Yannarelli, Gustavo Gabriel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv EXOSOMES
MESENCHYMAL STROMAL CELLS
BIOACTIVITY
INFLAMMATION
topic EXOSOMES
MESENCHYMAL STROMAL CELLS
BIOACTIVITY
INFLAMMATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Extracellular vesicles (EVs) play key roles in cell biology and may provide new clinical diagnostics and therapies. However, it has proven difficult to develop protocols for their purification and characterization. One of the major barriers in the field has been a lack of convenient assays for their bioactivity. Developing assays has not been a trivial matter, because of the heterogeneity of EVs, the multiple activities they demonstrate, and the uncertainty about their modes of action. Therefore, it is likely that multiple assays for their activities are needed. One important assay will be for the anti-inflammatory activity observed in mice after administration of the small EVs commonly referred to as exosomes. We developed an assay for the anti-inflammatory activity of exosomes with a line of mouse macrophages. The assay makes it possible to rank different preparations of exosomes by their anti-inflammatory activity, and their ranking predicts their efficacy in suppressing LPS-stimulated inflammation in mice. The assay is convenient for comparing multiple samples and, therefore, should be useful in developing protocols for the purification and characterization of anti-inflammatory exosomes.
Fil: Pacienza, Natalia Alejandra. Texas A&M University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina
Fil: Lee, Ryang Hwa. Texas A&M University; Estados Unidos
Fil: Bae, Eun-Hye. Texas A&M University; Estados Unidos
Fil: Kim, Dong-ki. Texas A&M University; Estados Unidos
Fil: Liu, Qisong. Texas A&M University; Estados Unidos
Fil: Prockop, Darwin J.. Texas A&M University; Estados Unidos
Fil: Yannarelli, Gustavo Gabriel. Texas A&M University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina
description Extracellular vesicles (EVs) play key roles in cell biology and may provide new clinical diagnostics and therapies. However, it has proven difficult to develop protocols for their purification and characterization. One of the major barriers in the field has been a lack of convenient assays for their bioactivity. Developing assays has not been a trivial matter, because of the heterogeneity of EVs, the multiple activities they demonstrate, and the uncertainty about their modes of action. Therefore, it is likely that multiple assays for their activities are needed. One important assay will be for the anti-inflammatory activity observed in mice after administration of the small EVs commonly referred to as exosomes. We developed an assay for the anti-inflammatory activity of exosomes with a line of mouse macrophages. The assay makes it possible to rank different preparations of exosomes by their anti-inflammatory activity, and their ranking predicts their efficacy in suppressing LPS-stimulated inflammation in mice. The assay is convenient for comparing multiple samples and, therefore, should be useful in developing protocols for the purification and characterization of anti-inflammatory exosomes.
publishDate 2019
dc.date.none.fl_str_mv 2019-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/121379
Pacienza, Natalia Alejandra; Lee, Ryang Hwa; Bae, Eun-Hye; Kim, Dong-ki; Liu, Qisong; et al.; In Vitro Macrophage Assay Predicts the In Vivo Anti-inflammatory Potential of Exosomes from Human Mesenchymal Stromal Cells; Cell Press; Molecular Therapy:Methods and Clinical Development; 13; 6-2019; 67-76
2329-0501
CONICET Digital
CONICET
url http://hdl.handle.net/11336/121379
identifier_str_mv Pacienza, Natalia Alejandra; Lee, Ryang Hwa; Bae, Eun-Hye; Kim, Dong-ki; Liu, Qisong; et al.; In Vitro Macrophage Assay Predicts the In Vivo Anti-inflammatory Potential of Exosomes from Human Mesenchymal Stromal Cells; Cell Press; Molecular Therapy:Methods and Clinical Development; 13; 6-2019; 67-76
2329-0501
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.omtm.2018.12.003
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cell Press
publisher.none.fl_str_mv Cell Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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