The potential role of epigenetics on multipotent cell differentiation capacity of mesenchymal stromal cells

Autores
Yannarelli, Gustavo Gabriel; Pacienza, Natalia Alejandra; Cuniberti, Luis Alberto; Medin, Jeffrey; Davies, John; Keating, Armand
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Human umbilical cord perivascular cells (HUCPVCs) are a readily available source of mesenchymal stromal cells (MSCs) for cell therapy. We were interested in understanding how differences from human bone marrow (BM)-derived MSCs might yield insights into MSC biology. We found that HUCPVCs exhibited increased telomerase activity and longer telomeres compared with BM-MSCs. We also observed enhanced expression of the pluripotency factors OCT4, SOX2, and NANOG in HUCPVCs. The methylation of OCT4 and NANOG promoters was similar in both cell types, indicating that differences in the expression of pluripotency factors between the MSCs were not associated with epigenetic changes. MSC methylation at these loci is greater than reported for embryonic stem cells but less than in dermal fibroblasts, suggesting that multipotentiality of MSCs is epigenetically restricted. These results are consistent with the notion that the MSC population (whether BM- or HUCPV-derived) exhibits higher proliferative capacity and contains more progenitor cells than do dermal fibroblasts.
Fil: Yannarelli, Gustavo Gabriel. Universidad Favaloro; Argentina. University Health Network; Canadá. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pacienza, Natalia Alejandra. University Health Network; Canadá. Universidad Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cuniberti, Luis Alberto. Universidad Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Medin, Jeffrey. University Health Network; Canadá
Fil: Davies, John. University of Toronto; Canadá
Fil: Keating, Armand. University Health Network; Canadá. University of Toronto; Canadá
Materia
Mesenchymal Stromal Cells
Telomerase
Pluripotency Factors
Epigenetics
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/22083
Acceder
id CONICETDig_5cbbbe911cc75defcad19dd521045614
oai_identifier_str oai:ri.conicet.gov.ar:11336/22083
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The potential role of epigenetics on multipotent cell differentiation capacity of mesenchymal stromal cellsYannarelli, Gustavo GabrielPacienza, Natalia AlejandraCuniberti, Luis AlbertoMedin, JeffreyDavies, JohnKeating, ArmandMesenchymal Stromal CellsTelomerasePluripotency FactorsEpigeneticshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Human umbilical cord perivascular cells (HUCPVCs) are a readily available source of mesenchymal stromal cells (MSCs) for cell therapy. We were interested in understanding how differences from human bone marrow (BM)-derived MSCs might yield insights into MSC biology. We found that HUCPVCs exhibited increased telomerase activity and longer telomeres compared with BM-MSCs. We also observed enhanced expression of the pluripotency factors OCT4, SOX2, and NANOG in HUCPVCs. The methylation of OCT4 and NANOG promoters was similar in both cell types, indicating that differences in the expression of pluripotency factors between the MSCs were not associated with epigenetic changes. MSC methylation at these loci is greater than reported for embryonic stem cells but less than in dermal fibroblasts, suggesting that multipotentiality of MSCs is epigenetically restricted. These results are consistent with the notion that the MSC population (whether BM- or HUCPV-derived) exhibits higher proliferative capacity and contains more progenitor cells than do dermal fibroblasts.Fil: Yannarelli, Gustavo Gabriel. Universidad Favaloro; Argentina. University Health Network; Canadá. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pacienza, Natalia Alejandra. University Health Network; Canadá. Universidad Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cuniberti, Luis Alberto. Universidad Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Medin, Jeffrey. University Health Network; CanadáFil: Davies, John. University of Toronto; CanadáFil: Keating, Armand. University Health Network; Canadá. University of Toronto; CanadáWiley2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/22083Yannarelli, Gustavo Gabriel; Pacienza, Natalia Alejandra; Cuniberti, Luis Alberto; Medin, Jeffrey; Davies, John; et al.; The potential role of epigenetics on multipotent cell differentiation capacity of mesenchymal stromal cells; Wiley; Stem Cells; 31; 1; 1-2013; 215-2201066-50991549-4918CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/stem.1262/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1002/stem.1262info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:50:27Zoai:ri.conicet.gov.ar:11336/22083instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:50:27.927CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The potential role of epigenetics on multipotent cell differentiation capacity of mesenchymal stromal cells
title The potential role of epigenetics on multipotent cell differentiation capacity of mesenchymal stromal cells
spellingShingle The potential role of epigenetics on multipotent cell differentiation capacity of mesenchymal stromal cells
Yannarelli, Gustavo Gabriel
Mesenchymal Stromal Cells
Telomerase
Pluripotency Factors
Epigenetics
title_short The potential role of epigenetics on multipotent cell differentiation capacity of mesenchymal stromal cells
title_full The potential role of epigenetics on multipotent cell differentiation capacity of mesenchymal stromal cells
title_fullStr The potential role of epigenetics on multipotent cell differentiation capacity of mesenchymal stromal cells
title_full_unstemmed The potential role of epigenetics on multipotent cell differentiation capacity of mesenchymal stromal cells
title_sort The potential role of epigenetics on multipotent cell differentiation capacity of mesenchymal stromal cells
dc.creator.none.fl_str_mv Yannarelli, Gustavo Gabriel
Pacienza, Natalia Alejandra
Cuniberti, Luis Alberto
Medin, Jeffrey
Davies, John
Keating, Armand
author Yannarelli, Gustavo Gabriel
author_facet Yannarelli, Gustavo Gabriel
Pacienza, Natalia Alejandra
Cuniberti, Luis Alberto
Medin, Jeffrey
Davies, John
Keating, Armand
author_role author
author2 Pacienza, Natalia Alejandra
Cuniberti, Luis Alberto
Medin, Jeffrey
Davies, John
Keating, Armand
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Mesenchymal Stromal Cells
Telomerase
Pluripotency Factors
Epigenetics
topic Mesenchymal Stromal Cells
Telomerase
Pluripotency Factors
Epigenetics
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Human umbilical cord perivascular cells (HUCPVCs) are a readily available source of mesenchymal stromal cells (MSCs) for cell therapy. We were interested in understanding how differences from human bone marrow (BM)-derived MSCs might yield insights into MSC biology. We found that HUCPVCs exhibited increased telomerase activity and longer telomeres compared with BM-MSCs. We also observed enhanced expression of the pluripotency factors OCT4, SOX2, and NANOG in HUCPVCs. The methylation of OCT4 and NANOG promoters was similar in both cell types, indicating that differences in the expression of pluripotency factors between the MSCs were not associated with epigenetic changes. MSC methylation at these loci is greater than reported for embryonic stem cells but less than in dermal fibroblasts, suggesting that multipotentiality of MSCs is epigenetically restricted. These results are consistent with the notion that the MSC population (whether BM- or HUCPV-derived) exhibits higher proliferative capacity and contains more progenitor cells than do dermal fibroblasts.
Fil: Yannarelli, Gustavo Gabriel. Universidad Favaloro; Argentina. University Health Network; Canadá. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pacienza, Natalia Alejandra. University Health Network; Canadá. Universidad Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cuniberti, Luis Alberto. Universidad Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Medin, Jeffrey. University Health Network; Canadá
Fil: Davies, John. University of Toronto; Canadá
Fil: Keating, Armand. University Health Network; Canadá. University of Toronto; Canadá
description Human umbilical cord perivascular cells (HUCPVCs) are a readily available source of mesenchymal stromal cells (MSCs) for cell therapy. We were interested in understanding how differences from human bone marrow (BM)-derived MSCs might yield insights into MSC biology. We found that HUCPVCs exhibited increased telomerase activity and longer telomeres compared with BM-MSCs. We also observed enhanced expression of the pluripotency factors OCT4, SOX2, and NANOG in HUCPVCs. The methylation of OCT4 and NANOG promoters was similar in both cell types, indicating that differences in the expression of pluripotency factors between the MSCs were not associated with epigenetic changes. MSC methylation at these loci is greater than reported for embryonic stem cells but less than in dermal fibroblasts, suggesting that multipotentiality of MSCs is epigenetically restricted. These results are consistent with the notion that the MSC population (whether BM- or HUCPV-derived) exhibits higher proliferative capacity and contains more progenitor cells than do dermal fibroblasts.
publishDate 2013
dc.date.none.fl_str_mv 2013-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/22083
Yannarelli, Gustavo Gabriel; Pacienza, Natalia Alejandra; Cuniberti, Luis Alberto; Medin, Jeffrey; Davies, John; et al.; The potential role of epigenetics on multipotent cell differentiation capacity of mesenchymal stromal cells; Wiley; Stem Cells; 31; 1; 1-2013; 215-220
1066-5099
1549-4918
CONICET Digital
CONICET
url http://hdl.handle.net/11336/22083
identifier_str_mv Yannarelli, Gustavo Gabriel; Pacienza, Natalia Alejandra; Cuniberti, Luis Alberto; Medin, Jeffrey; Davies, John; et al.; The potential role of epigenetics on multipotent cell differentiation capacity of mesenchymal stromal cells; Wiley; Stem Cells; 31; 1; 1-2013; 215-220
1066-5099
1549-4918
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/stem.1262/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1002/stem.1262
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083029260304384
score 13.22299

Publicaciones similares