Recombinant protein expression in microbial systems

Autores
Rosano, German Leandro; Ceccarelli, Eduardo Augusto
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The emergence of recombinant DNA technology during the early 70's set a revolution in molecular biology. This set of techniques was strengthened even further later on with the introduction of the polymerase chain reaction and allowed scientists to explore and understand essential life processes in an easy and straightforward way. It also marked the birth of the modern biotech industry. At that time, it was shown that eukaryotic DNA could be propagated in Escherichia coli (Morrow et al., 1974) and functional products could be synthesized from heterologous genes cloned in bacterial plasmids (Ratzkin and Carbon, 1977; Vapnek et al., 1977). After these successful cases, it was soon realized that the potential applications of these techniques were almost limitless. In fact, US patent 4,237,224 granted to Cohen and Boyer (1980) claimed to commercial ownership of the methodology for cloning virtually all possible DNAs in all possible vectors. While cloning any gene in any given vector is feasible, obtaining a functional product from its expression is not that simple.
Fil: Rosano, German Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Ceccarelli, Eduardo Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Materia
RECOMBINANT PROTEINS
MICROORGANISM
INCLUSION BODIES
FUSION TAGS
ESCHERICHIA COLI
YEAST
FILAMENTOUS FUNGI
MICROALGAE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/21362
Acceder
id CONICETDig_553736c0b03417b793b3e6327a7a0f10
oai_identifier_str oai:ri.conicet.gov.ar:11336/21362
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Recombinant protein expression in microbial systemsRosano, German LeandroCeccarelli, Eduardo AugustoRECOMBINANT PROTEINSMICROORGANISMINCLUSION BODIESFUSION TAGSESCHERICHIA COLIYEASTFILAMENTOUS FUNGIMICROALGAEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The emergence of recombinant DNA technology during the early 70's set a revolution in molecular biology. This set of techniques was strengthened even further later on with the introduction of the polymerase chain reaction and allowed scientists to explore and understand essential life processes in an easy and straightforward way. It also marked the birth of the modern biotech industry. At that time, it was shown that eukaryotic DNA could be propagated in Escherichia coli (Morrow et al., 1974) and functional products could be synthesized from heterologous genes cloned in bacterial plasmids (Ratzkin and Carbon, 1977; Vapnek et al., 1977). After these successful cases, it was soon realized that the potential applications of these techniques were almost limitless. In fact, US patent 4,237,224 granted to Cohen and Boyer (1980) claimed to commercial ownership of the methodology for cloning virtually all possible DNAs in all possible vectors. While cloning any gene in any given vector is feasible, obtaining a functional product from its expression is not that simple.Fil: Rosano, German Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Ceccarelli, Eduardo Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFrontiers2014-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/21362Rosano, German Leandro; Ceccarelli, Eduardo Augusto; Recombinant protein expression in microbial systems; Frontiers; Frontiers in Microbiology; 5; 7-2014; 1-2; 3411664-302XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2014.00341info:eu-repo/semantics/altIdentifier/url/http://journal.frontiersin.org/article/10.3389/fmicb.2014.00341/fullinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-26T08:55:57Zoai:ri.conicet.gov.ar:11336/21362instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-26 08:55:57.313CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Recombinant protein expression in microbial systems
title Recombinant protein expression in microbial systems
spellingShingle Recombinant protein expression in microbial systems
Rosano, German Leandro
RECOMBINANT PROTEINS
MICROORGANISM
INCLUSION BODIES
FUSION TAGS
ESCHERICHIA COLI
YEAST
FILAMENTOUS FUNGI
MICROALGAE
title_short Recombinant protein expression in microbial systems
title_full Recombinant protein expression in microbial systems
title_fullStr Recombinant protein expression in microbial systems
title_full_unstemmed Recombinant protein expression in microbial systems
title_sort Recombinant protein expression in microbial systems
dc.creator.none.fl_str_mv Rosano, German Leandro
Ceccarelli, Eduardo Augusto
author Rosano, German Leandro
author_facet Rosano, German Leandro
Ceccarelli, Eduardo Augusto
author_role author
author2 Ceccarelli, Eduardo Augusto
author2_role author
dc.subject.none.fl_str_mv RECOMBINANT PROTEINS
MICROORGANISM
INCLUSION BODIES
FUSION TAGS
ESCHERICHIA COLI
YEAST
FILAMENTOUS FUNGI
MICROALGAE
topic RECOMBINANT PROTEINS
MICROORGANISM
INCLUSION BODIES
FUSION TAGS
ESCHERICHIA COLI
YEAST
FILAMENTOUS FUNGI
MICROALGAE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The emergence of recombinant DNA technology during the early 70's set a revolution in molecular biology. This set of techniques was strengthened even further later on with the introduction of the polymerase chain reaction and allowed scientists to explore and understand essential life processes in an easy and straightforward way. It also marked the birth of the modern biotech industry. At that time, it was shown that eukaryotic DNA could be propagated in Escherichia coli (Morrow et al., 1974) and functional products could be synthesized from heterologous genes cloned in bacterial plasmids (Ratzkin and Carbon, 1977; Vapnek et al., 1977). After these successful cases, it was soon realized that the potential applications of these techniques were almost limitless. In fact, US patent 4,237,224 granted to Cohen and Boyer (1980) claimed to commercial ownership of the methodology for cloning virtually all possible DNAs in all possible vectors. While cloning any gene in any given vector is feasible, obtaining a functional product from its expression is not that simple.
Fil: Rosano, German Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Ceccarelli, Eduardo Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
description The emergence of recombinant DNA technology during the early 70's set a revolution in molecular biology. This set of techniques was strengthened even further later on with the introduction of the polymerase chain reaction and allowed scientists to explore and understand essential life processes in an easy and straightforward way. It also marked the birth of the modern biotech industry. At that time, it was shown that eukaryotic DNA could be propagated in Escherichia coli (Morrow et al., 1974) and functional products could be synthesized from heterologous genes cloned in bacterial plasmids (Ratzkin and Carbon, 1977; Vapnek et al., 1977). After these successful cases, it was soon realized that the potential applications of these techniques were almost limitless. In fact, US patent 4,237,224 granted to Cohen and Boyer (1980) claimed to commercial ownership of the methodology for cloning virtually all possible DNAs in all possible vectors. While cloning any gene in any given vector is feasible, obtaining a functional product from its expression is not that simple.
publishDate 2014
dc.date.none.fl_str_mv 2014-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/21362
Rosano, German Leandro; Ceccarelli, Eduardo Augusto; Recombinant protein expression in microbial systems; Frontiers; Frontiers in Microbiology; 5; 7-2014; 1-2; 341
1664-302X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/21362
identifier_str_mv Rosano, German Leandro; Ceccarelli, Eduardo Augusto; Recombinant protein expression in microbial systems; Frontiers; Frontiers in Microbiology; 5; 7-2014; 1-2; 341
1664-302X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2014.00341
info:eu-repo/semantics/altIdentifier/url/http://journal.frontiersin.org/article/10.3389/fmicb.2014.00341/full
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers
publisher.none.fl_str_mv Frontiers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1849873125603278848
score 13.011256

Publicaciones similares