Recombinant protein expression in Escherichia coli: advances and challenges

Autores
Rosano, German Leandro; Ceccarelli, Eduardo Augusto
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Escherichia coli is one of the organisms of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform. For this reason, there are many molecular tools and protocols at hand for the high-level production of heterologous proteins, such as a vast catalog of expression plasmids, a great number of engineered strains and many cultivation strategies.We review the different approaches for the synthesis of recombinant proteins in E. coli and discuss recent progress in this ever-growing field.
Fil: Rosano, German Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario, Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Ceccarelli, Eduardo Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario, Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Materia
Expresión de proteínas
Proteínas heterólogas
Plásmidos de expresión
Escherichia coli
Recombinant protein expression
Expression plasmid
Inclusion bodies
Affinity tags
E. coli expression strains
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8821
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Recombinant protein expression in Escherichia coli: advances and challengesRosano, German LeandroCeccarelli, Eduardo AugustoExpresión de proteínasProteínas heterólogasPlásmidos de expresiónEscherichia coliRecombinant protein expressionExpression plasmidInclusion bodiesAffinity tagsE. coli expression strainshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Escherichia coli is one of the organisms of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform. For this reason, there are many molecular tools and protocols at hand for the high-level production of heterologous proteins, such as a vast catalog of expression plasmids, a great number of engineered strains and many cultivation strategies.We review the different approaches for the synthesis of recombinant proteins in E. coli and discuss recent progress in this ever-growing field.Fil: Rosano, German Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario, Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Ceccarelli, Eduardo Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario, Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFrontiers2014-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8821Rosano, German Leandro; Ceccarelli, Eduardo Augusto; Recombinant protein expression in Escherichia coli: advances and challenges; Frontiers; Frontiers in Microbiology; 5; 4-2014; 1-17; 1721664-302Xenginfo:eu-repo/semantics/altIdentifier/url/http://www.frontiersin.org/Journal/Abstract.aspx?s=679&name=microbiotechnology,_ecotoxicology_and_bioremediation&ART_DOI=10.3389/fmicb.2014.00172info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2014.00172info:eu-repo/semantics/altIdentifier/pmid/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4029002/info:eu-repo/semantics/altIdentifier/url/https://doaj.org/article/d515e752014c4329afbe7ac76820c696info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-26T08:55:47Zoai:ri.conicet.gov.ar:11336/8821instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-26 08:55:47.239CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Recombinant protein expression in Escherichia coli: advances and challenges
title Recombinant protein expression in Escherichia coli: advances and challenges
spellingShingle Recombinant protein expression in Escherichia coli: advances and challenges
Rosano, German Leandro
Expresión de proteínas
Proteínas heterólogas
Plásmidos de expresión
Escherichia coli
Recombinant protein expression
Expression plasmid
Inclusion bodies
Affinity tags
E. coli expression strains
title_short Recombinant protein expression in Escherichia coli: advances and challenges
title_full Recombinant protein expression in Escherichia coli: advances and challenges
title_fullStr Recombinant protein expression in Escherichia coli: advances and challenges
title_full_unstemmed Recombinant protein expression in Escherichia coli: advances and challenges
title_sort Recombinant protein expression in Escherichia coli: advances and challenges
dc.creator.none.fl_str_mv Rosano, German Leandro
Ceccarelli, Eduardo Augusto
author Rosano, German Leandro
author_facet Rosano, German Leandro
Ceccarelli, Eduardo Augusto
author_role author
author2 Ceccarelli, Eduardo Augusto
author2_role author
dc.subject.none.fl_str_mv Expresión de proteínas
Proteínas heterólogas
Plásmidos de expresión
Escherichia coli
Recombinant protein expression
Expression plasmid
Inclusion bodies
Affinity tags
E. coli expression strains
topic Expresión de proteínas
Proteínas heterólogas
Plásmidos de expresión
Escherichia coli
Recombinant protein expression
Expression plasmid
Inclusion bodies
Affinity tags
E. coli expression strains
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Escherichia coli is one of the organisms of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform. For this reason, there are many molecular tools and protocols at hand for the high-level production of heterologous proteins, such as a vast catalog of expression plasmids, a great number of engineered strains and many cultivation strategies.We review the different approaches for the synthesis of recombinant proteins in E. coli and discuss recent progress in this ever-growing field.
Fil: Rosano, German Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario, Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Ceccarelli, Eduardo Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario, Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
description Escherichia coli is one of the organisms of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform. For this reason, there are many molecular tools and protocols at hand for the high-level production of heterologous proteins, such as a vast catalog of expression plasmids, a great number of engineered strains and many cultivation strategies.We review the different approaches for the synthesis of recombinant proteins in E. coli and discuss recent progress in this ever-growing field.
publishDate 2014
dc.date.none.fl_str_mv 2014-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8821
Rosano, German Leandro; Ceccarelli, Eduardo Augusto; Recombinant protein expression in Escherichia coli: advances and challenges; Frontiers; Frontiers in Microbiology; 5; 4-2014; 1-17; 172
1664-302X
url http://hdl.handle.net/11336/8821
identifier_str_mv Rosano, German Leandro; Ceccarelli, Eduardo Augusto; Recombinant protein expression in Escherichia coli: advances and challenges; Frontiers; Frontiers in Microbiology; 5; 4-2014; 1-17; 172
1664-302X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.frontiersin.org/Journal/Abstract.aspx?s=679&name=microbiotechnology,_ecotoxicology_and_bioremediation&ART_DOI=10.3389/fmicb.2014.00172
info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2014.00172
info:eu-repo/semantics/altIdentifier/pmid/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4029002/
info:eu-repo/semantics/altIdentifier/url/https://doaj.org/article/d515e752014c4329afbe7ac76820c696
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers
publisher.none.fl_str_mv Frontiers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.011256

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