Rapid screening of statin-producing filamentous fungi

Autores
Cabral, Maria Eugenia; Castellanos, Lucia Ines; Siñeriz, Faustino; Fariña, Julia Ines
Año de publicación
2007
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Hypercholesterolemia, an important risk factor in coronary artery disease, is nowadays commonly treated with statins. They are a class of fungal secondary metabolites that can inhibit the de novo synthesis of endogenous cholesterol by means of the competitive inhibition of HMG-CoA reductase. From 14-day-old fungal cultures cultivated at 20°C on lactose-yeast extract agar medium, statins were extracted with ethyl acetate at 50°C for 20 min with agitation. Two different methods were applied: a) Glass beads disruption: mycelium-covered agar plugs were transferred to a test tube and subsequently treated with glass beads and ethyl acetate. b) Freeze-thawing: mycelium-covered agar plugs were freezed overnight and, after thawing, supernatant was recovered by centrifugation and mixed with an equal volume of ethyl acetate. Presence of statins in the organic phase was simultaneously evaluated by both thin layer chromatography (TLC) and a bioassay based on the yeast growth-inhibitory effect (either with Candida albicans or Saccharomyces cerevisiae. For bioassay, actively growing yeasts were included in molten YNB agar medium, poured into plates and get solidified. Statin extracts (25 ul were poured into 6mm-diam. wells and compared to standard statins. After incubation at 26°, inhibition haloes formation was assessed. Results according to both methodologies revealed that 23 isolates from 230 filamentous fungi collected from Las Yungas (Tucumán, Argentina) would be able to produce bioactive compounds compatible with statins. Applied protocols showed to be valid at the time of selecting statin-producers, allowing high sensitivity and readily available results in association with the ease of sample processing.
Fil: Cabral, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Siñeriz, Faustino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Society for Industrial Microbiology Annual Meeting and Exhibition
Denver
Estados Unidos
Society for Industrial Microbiology
Materia
SCREENING
STATIN
FILAMENTOUS FUNGI
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/200206
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/200206
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Rapid screening of statin-producing filamentous fungiCabral, Maria EugeniaCastellanos, Lucia InesSiñeriz, FaustinoFariña, Julia InesSCREENINGSTATINFILAMENTOUS FUNGIhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Hypercholesterolemia, an important risk factor in coronary artery disease, is nowadays commonly treated with statins. They are a class of fungal secondary metabolites that can inhibit the de novo synthesis of endogenous cholesterol by means of the competitive inhibition of HMG-CoA reductase. From 14-day-old fungal cultures cultivated at 20°C on lactose-yeast extract agar medium, statins were extracted with ethyl acetate at 50°C for 20 min with agitation. Two different methods were applied: a) Glass beads disruption: mycelium-covered agar plugs were transferred to a test tube and subsequently treated with glass beads and ethyl acetate. b) Freeze-thawing: mycelium-covered agar plugs were freezed overnight and, after thawing, supernatant was recovered by centrifugation and mixed with an equal volume of ethyl acetate. Presence of statins in the organic phase was simultaneously evaluated by both thin layer chromatography (TLC) and a bioassay based on the yeast growth-inhibitory effect (either with Candida albicans or Saccharomyces cerevisiae. For bioassay, actively growing yeasts were included in molten YNB agar medium, poured into plates and get solidified. Statin extracts (25 ul were poured into 6mm-diam. wells and compared to standard statins. After incubation at 26°, inhibition haloes formation was assessed. Results according to both methodologies revealed that 23 isolates from 230 filamentous fungi collected from Las Yungas (Tucumán, Argentina) would be able to produce bioactive compounds compatible with statins. Applied protocols showed to be valid at the time of selecting statin-producers, allowing high sensitivity and readily available results in association with the ease of sample processing.Fil: Cabral, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Siñeriz, Faustino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaSociety for Industrial Microbiology Annual Meeting and ExhibitionDenverEstados UnidosSociety for Industrial MicrobiologySociety for Industrial Microbiology2007info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/200206Rapid screening of statin-producing filamentous fungi; Society for Industrial Microbiology Annual Meeting and Exhibition; Denver; Estados Unidos; 2007; 1-1CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sim.confex.com/sim/2007/techprogram/P3796.HTMInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:24Zoai:ri.conicet.gov.ar:11336/200206instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:24.851CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Rapid screening of statin-producing filamentous fungi
title Rapid screening of statin-producing filamentous fungi
spellingShingle Rapid screening of statin-producing filamentous fungi
Cabral, Maria Eugenia
SCREENING
STATIN
FILAMENTOUS FUNGI
title_short Rapid screening of statin-producing filamentous fungi
title_full Rapid screening of statin-producing filamentous fungi
title_fullStr Rapid screening of statin-producing filamentous fungi
title_full_unstemmed Rapid screening of statin-producing filamentous fungi
title_sort Rapid screening of statin-producing filamentous fungi
dc.creator.none.fl_str_mv Cabral, Maria Eugenia
Castellanos, Lucia Ines
Siñeriz, Faustino
Fariña, Julia Ines
author Cabral, Maria Eugenia
author_facet Cabral, Maria Eugenia
Castellanos, Lucia Ines
Siñeriz, Faustino
Fariña, Julia Ines
author_role author
author2 Castellanos, Lucia Ines
Siñeriz, Faustino
Fariña, Julia Ines
author2_role author
author
author
dc.subject.none.fl_str_mv SCREENING
STATIN
FILAMENTOUS FUNGI
topic SCREENING
STATIN
FILAMENTOUS FUNGI
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Hypercholesterolemia, an important risk factor in coronary artery disease, is nowadays commonly treated with statins. They are a class of fungal secondary metabolites that can inhibit the de novo synthesis of endogenous cholesterol by means of the competitive inhibition of HMG-CoA reductase. From 14-day-old fungal cultures cultivated at 20°C on lactose-yeast extract agar medium, statins were extracted with ethyl acetate at 50°C for 20 min with agitation. Two different methods were applied: a) Glass beads disruption: mycelium-covered agar plugs were transferred to a test tube and subsequently treated with glass beads and ethyl acetate. b) Freeze-thawing: mycelium-covered agar plugs were freezed overnight and, after thawing, supernatant was recovered by centrifugation and mixed with an equal volume of ethyl acetate. Presence of statins in the organic phase was simultaneously evaluated by both thin layer chromatography (TLC) and a bioassay based on the yeast growth-inhibitory effect (either with Candida albicans or Saccharomyces cerevisiae. For bioassay, actively growing yeasts were included in molten YNB agar medium, poured into plates and get solidified. Statin extracts (25 ul were poured into 6mm-diam. wells and compared to standard statins. After incubation at 26°, inhibition haloes formation was assessed. Results according to both methodologies revealed that 23 isolates from 230 filamentous fungi collected from Las Yungas (Tucumán, Argentina) would be able to produce bioactive compounds compatible with statins. Applied protocols showed to be valid at the time of selecting statin-producers, allowing high sensitivity and readily available results in association with the ease of sample processing.
Fil: Cabral, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Siñeriz, Faustino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Society for Industrial Microbiology Annual Meeting and Exhibition
Denver
Estados Unidos
Society for Industrial Microbiology
description Hypercholesterolemia, an important risk factor in coronary artery disease, is nowadays commonly treated with statins. They are a class of fungal secondary metabolites that can inhibit the de novo synthesis of endogenous cholesterol by means of the competitive inhibition of HMG-CoA reductase. From 14-day-old fungal cultures cultivated at 20°C on lactose-yeast extract agar medium, statins were extracted with ethyl acetate at 50°C for 20 min with agitation. Two different methods were applied: a) Glass beads disruption: mycelium-covered agar plugs were transferred to a test tube and subsequently treated with glass beads and ethyl acetate. b) Freeze-thawing: mycelium-covered agar plugs were freezed overnight and, after thawing, supernatant was recovered by centrifugation and mixed with an equal volume of ethyl acetate. Presence of statins in the organic phase was simultaneously evaluated by both thin layer chromatography (TLC) and a bioassay based on the yeast growth-inhibitory effect (either with Candida albicans or Saccharomyces cerevisiae. For bioassay, actively growing yeasts were included in molten YNB agar medium, poured into plates and get solidified. Statin extracts (25 ul were poured into 6mm-diam. wells and compared to standard statins. After incubation at 26°, inhibition haloes formation was assessed. Results according to both methodologies revealed that 23 isolates from 230 filamentous fungi collected from Las Yungas (Tucumán, Argentina) would be able to produce bioactive compounds compatible with statins. Applied protocols showed to be valid at the time of selecting statin-producers, allowing high sensitivity and readily available results in association with the ease of sample processing.
publishDate 2007
dc.date.none.fl_str_mv 2007
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/200206
Rapid screening of statin-producing filamentous fungi; Society for Industrial Microbiology Annual Meeting and Exhibition; Denver; Estados Unidos; 2007; 1-1
CONICET Digital
CONICET
url http://hdl.handle.net/11336/200206
identifier_str_mv Rapid screening of statin-producing filamentous fungi; Society for Industrial Microbiology Annual Meeting and Exhibition; Denver; Estados Unidos; 2007; 1-1
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://sim.confex.com/sim/2007/techprogram/P3796.HTM
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
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application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Society for Industrial Microbiology
publisher.none.fl_str_mv Society for Industrial Microbiology
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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