New tools for recombinant protein production in Escherichia coli : A 5‐year update

Autores
Rosano, German Leandro; Morales, Enrique Salvador; Ceccarelli, Eduardo Augusto
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The production of proteins in sufficient amounts is key for their study or use as biotherapeutic agents. Escherichia coli is the host of choice for recombinant protein production given its fast growth, easy manipulation, and cost-effectiveness. As such, its protein production capabilities are continuously being improved. Also, the associated tools (such as plasmids and cultivation conditions) are subject of ongoing research to optimize product yield. In this work, we review the latest advances in recombinant protein production in E. coli.
Fil: Rosano, German Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Morales, Enrique Salvador. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Ceccarelli, Eduardo Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Materia
AFFINITY TAGS
E. COLI STRAINS
ESCHERICHIA COLI
EXPRESSION PLASMID
FUSION PARTNERS
INCLUSION BODIES
RECOMBINANT PROTEIN PRODUCTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/151837
Acceder
id CONICETDig_3843785e5697ac3b91b3fc013bcef15a
oai_identifier_str oai:ri.conicet.gov.ar:11336/151837
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling New tools for recombinant protein production in Escherichia coli : A 5‐year updateRosano, German LeandroMorales, Enrique SalvadorCeccarelli, Eduardo AugustoAFFINITY TAGSE. COLI STRAINSESCHERICHIA COLIEXPRESSION PLASMIDFUSION PARTNERSINCLUSION BODIESRECOMBINANT PROTEIN PRODUCTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The production of proteins in sufficient amounts is key for their study or use as biotherapeutic agents. Escherichia coli is the host of choice for recombinant protein production given its fast growth, easy manipulation, and cost-effectiveness. As such, its protein production capabilities are continuously being improved. Also, the associated tools (such as plasmids and cultivation conditions) are subject of ongoing research to optimize product yield. In this work, we review the latest advances in recombinant protein production in E. coli.Fil: Rosano, German Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Morales, Enrique Salvador. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Ceccarelli, Eduardo Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaJohn Wiley & Sons Inc2019-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/151837Rosano, German Leandro; Morales, Enrique Salvador; Ceccarelli, Eduardo Augusto; New tools for recombinant protein production in Escherichia coli : A 5‐year update; John Wiley & Sons Inc; Protein Science; 28; 8; 7-2019; 1412-14220961-8368CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/pro.3668info:eu-repo/semantics/altIdentifier/doi/10.1002/pro.3668info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-26T08:40:04Zoai:ri.conicet.gov.ar:11336/151837instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-26 08:40:04.323CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv New tools for recombinant protein production in Escherichia coli : A 5‐year update
title New tools for recombinant protein production in Escherichia coli : A 5‐year update
spellingShingle New tools for recombinant protein production in Escherichia coli : A 5‐year update
Rosano, German Leandro
AFFINITY TAGS
E. COLI STRAINS
ESCHERICHIA COLI
EXPRESSION PLASMID
FUSION PARTNERS
INCLUSION BODIES
RECOMBINANT PROTEIN PRODUCTION
title_short New tools for recombinant protein production in Escherichia coli : A 5‐year update
title_full New tools for recombinant protein production in Escherichia coli : A 5‐year update
title_fullStr New tools for recombinant protein production in Escherichia coli : A 5‐year update
title_full_unstemmed New tools for recombinant protein production in Escherichia coli : A 5‐year update
title_sort New tools for recombinant protein production in Escherichia coli : A 5‐year update
dc.creator.none.fl_str_mv Rosano, German Leandro
Morales, Enrique Salvador
Ceccarelli, Eduardo Augusto
author Rosano, German Leandro
author_facet Rosano, German Leandro
Morales, Enrique Salvador
Ceccarelli, Eduardo Augusto
author_role author
author2 Morales, Enrique Salvador
Ceccarelli, Eduardo Augusto
author2_role author
author
dc.subject.none.fl_str_mv AFFINITY TAGS
E. COLI STRAINS
ESCHERICHIA COLI
EXPRESSION PLASMID
FUSION PARTNERS
INCLUSION BODIES
RECOMBINANT PROTEIN PRODUCTION
topic AFFINITY TAGS
E. COLI STRAINS
ESCHERICHIA COLI
EXPRESSION PLASMID
FUSION PARTNERS
INCLUSION BODIES
RECOMBINANT PROTEIN PRODUCTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The production of proteins in sufficient amounts is key for their study or use as biotherapeutic agents. Escherichia coli is the host of choice for recombinant protein production given its fast growth, easy manipulation, and cost-effectiveness. As such, its protein production capabilities are continuously being improved. Also, the associated tools (such as plasmids and cultivation conditions) are subject of ongoing research to optimize product yield. In this work, we review the latest advances in recombinant protein production in E. coli.
Fil: Rosano, German Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Morales, Enrique Salvador. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Ceccarelli, Eduardo Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
description The production of proteins in sufficient amounts is key for their study or use as biotherapeutic agents. Escherichia coli is the host of choice for recombinant protein production given its fast growth, easy manipulation, and cost-effectiveness. As such, its protein production capabilities are continuously being improved. Also, the associated tools (such as plasmids and cultivation conditions) are subject of ongoing research to optimize product yield. In this work, we review the latest advances in recombinant protein production in E. coli.
publishDate 2019
dc.date.none.fl_str_mv 2019-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/151837
Rosano, German Leandro; Morales, Enrique Salvador; Ceccarelli, Eduardo Augusto; New tools for recombinant protein production in Escherichia coli : A 5‐year update; John Wiley & Sons Inc; Protein Science; 28; 8; 7-2019; 1412-1422
0961-8368
CONICET Digital
CONICET
url http://hdl.handle.net/11336/151837
identifier_str_mv Rosano, German Leandro; Morales, Enrique Salvador; Ceccarelli, Eduardo Augusto; New tools for recombinant protein production in Escherichia coli : A 5‐year update; John Wiley & Sons Inc; Protein Science; 28; 8; 7-2019; 1412-1422
0961-8368
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/pro.3668
info:eu-repo/semantics/altIdentifier/doi/10.1002/pro.3668
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Inc
publisher.none.fl_str_mv John Wiley & Sons Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1849872382584422400
score 13.011256

Publicaciones similares