A specific transitory increase in intracellular calcium induced by progesterone promotes acrosomal exocytosis in mouse sperm

Autores
Romarowski, Ana; Sánchez Cárdenas, Claudia; Ramírez Gómez, Héctor V.; Puga Molina, Lis del Carmen; Treviño, Claudia L.; Hernández Cruz, Arturo; Darszon, Alberto I.; Buffone, Mariano Gabriel
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
During capacitation, sperm acquire the ability to undergo the acrosome reaction (AR), an essential step in fertilization. Progesterone produced by cumulus cells has been associated with various physiological processes in sperm, including stimulation of AR. An increase in intracellular Ca2+ ([Ca2+]i) is necessary for AR to occur. In this study, we investigated the spatio- temporal correlation between the changes in [Ca2+]i and AR in single mouse spermatozoa in response to Progesterone. We found that Progesterone stimulates an [Ca2+]i increase in five different patterns: gradual increase, oscillatory, late transitory, immediate transitory and sustained. We also observed that the [Ca2+]i increase promoted by Progesterone starts at either the flagellum or the head. We validated the use of FM4-64 as an indicator for the occurrence of the AR by simultaneously detecting its fluorescence increase and the loss of EGFP in transgenic EGFPAcr sperm. For the first time, we have simultaneously visualized the rise in [Ca2+]i and the process of exocytosis in response to Progesterone and found that only a specific transitory increase in [Ca2+]i originated in the sperm head promotes the initiation of AR.
Fil: Romarowski, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Sánchez Cárdenas, Claudia. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Ramírez Gómez, Héctor V.. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Puga Molina, Lis del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Treviño, Claudia L.. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Hernández Cruz, Arturo. Universidad Nacional Autónoma de México. Instituto de Fisiología Celular; México
Fil: Darszon, Alberto I.. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Materia
Sperm
Acrosomal Exocytosis
Calcium
Progesterone
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/23038
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/23038
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A specific transitory increase in intracellular calcium induced by progesterone promotes acrosomal exocytosis in mouse spermRomarowski, AnaSánchez Cárdenas, ClaudiaRamírez Gómez, Héctor V.Puga Molina, Lis del CarmenTreviño, Claudia L.Hernández Cruz, ArturoDarszon, Alberto I.Buffone, Mariano GabrielSpermAcrosomal ExocytosisCalciumProgesteronehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3During capacitation, sperm acquire the ability to undergo the acrosome reaction (AR), an essential step in fertilization. Progesterone produced by cumulus cells has been associated with various physiological processes in sperm, including stimulation of AR. An increase in intracellular Ca2+ ([Ca2+]i) is necessary for AR to occur. In this study, we investigated the spatio- temporal correlation between the changes in [Ca2+]i and AR in single mouse spermatozoa in response to Progesterone. We found that Progesterone stimulates an [Ca2+]i increase in five different patterns: gradual increase, oscillatory, late transitory, immediate transitory and sustained. We also observed that the [Ca2+]i increase promoted by Progesterone starts at either the flagellum or the head. We validated the use of FM4-64 as an indicator for the occurrence of the AR by simultaneously detecting its fluorescence increase and the loss of EGFP in transgenic EGFPAcr sperm. For the first time, we have simultaneously visualized the rise in [Ca2+]i and the process of exocytosis in response to Progesterone and found that only a specific transitory increase in [Ca2+]i originated in the sperm head promotes the initiation of AR.Fil: Romarowski, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Sánchez Cárdenas, Claudia. Universidad Nacional Autónoma de México. Instituto de Biotecnología; MéxicoFil: Ramírez Gómez, Héctor V.. Universidad Nacional Autónoma de México. Instituto de Biotecnología; MéxicoFil: Puga Molina, Lis del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Treviño, Claudia L.. Universidad Nacional Autónoma de México. Instituto de Biotecnología; MéxicoFil: Hernández Cruz, Arturo. Universidad Nacional Autónoma de México. Instituto de Fisiología Celular; MéxicoFil: Darszon, Alberto I.. Universidad Nacional Autónoma de México. Instituto de Biotecnología; MéxicoFil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaSociety for the Study of Reproduction2016-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/23038Romarowski, Ana; Sánchez Cárdenas, Claudia; Ramírez Gómez, Héctor V.; Puga Molina, Lis del Carmen; Treviño, Claudia L.; et al.; A specific transitory increase in intracellular calcium induced by progesterone promotes acrosomal exocytosis in mouse sperm; Society for the Study of Reproduction; Biology of Reproduction; 94; 3; 1-3-2016; 1-120006-33631529-7268CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolreprod/article-lookup/doi/10.1095/biolreprod.115.136085info:eu-repo/semantics/altIdentifier/doi/10.1095/biolreprod.115.136085info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4829090/info:eu-repo/semantics/altIdentifier/pmid/26819478info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:43Zoai:ri.conicet.gov.ar:11336/23038instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:43.52CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A specific transitory increase in intracellular calcium induced by progesterone promotes acrosomal exocytosis in mouse sperm
title A specific transitory increase in intracellular calcium induced by progesterone promotes acrosomal exocytosis in mouse sperm
spellingShingle A specific transitory increase in intracellular calcium induced by progesterone promotes acrosomal exocytosis in mouse sperm
Romarowski, Ana
Sperm
Acrosomal Exocytosis
Calcium
Progesterone
title_short A specific transitory increase in intracellular calcium induced by progesterone promotes acrosomal exocytosis in mouse sperm
title_full A specific transitory increase in intracellular calcium induced by progesterone promotes acrosomal exocytosis in mouse sperm
title_fullStr A specific transitory increase in intracellular calcium induced by progesterone promotes acrosomal exocytosis in mouse sperm
title_full_unstemmed A specific transitory increase in intracellular calcium induced by progesterone promotes acrosomal exocytosis in mouse sperm
title_sort A specific transitory increase in intracellular calcium induced by progesterone promotes acrosomal exocytosis in mouse sperm
dc.creator.none.fl_str_mv Romarowski, Ana
Sánchez Cárdenas, Claudia
Ramírez Gómez, Héctor V.
Puga Molina, Lis del Carmen
Treviño, Claudia L.
Hernández Cruz, Arturo
Darszon, Alberto I.
Buffone, Mariano Gabriel
author Romarowski, Ana
author_facet Romarowski, Ana
Sánchez Cárdenas, Claudia
Ramírez Gómez, Héctor V.
Puga Molina, Lis del Carmen
Treviño, Claudia L.
Hernández Cruz, Arturo
Darszon, Alberto I.
Buffone, Mariano Gabriel
author_role author
author2 Sánchez Cárdenas, Claudia
Ramírez Gómez, Héctor V.
Puga Molina, Lis del Carmen
Treviño, Claudia L.
Hernández Cruz, Arturo
Darszon, Alberto I.
Buffone, Mariano Gabriel
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Sperm
Acrosomal Exocytosis
Calcium
Progesterone
topic Sperm
Acrosomal Exocytosis
Calcium
Progesterone
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv During capacitation, sperm acquire the ability to undergo the acrosome reaction (AR), an essential step in fertilization. Progesterone produced by cumulus cells has been associated with various physiological processes in sperm, including stimulation of AR. An increase in intracellular Ca2+ ([Ca2+]i) is necessary for AR to occur. In this study, we investigated the spatio- temporal correlation between the changes in [Ca2+]i and AR in single mouse spermatozoa in response to Progesterone. We found that Progesterone stimulates an [Ca2+]i increase in five different patterns: gradual increase, oscillatory, late transitory, immediate transitory and sustained. We also observed that the [Ca2+]i increase promoted by Progesterone starts at either the flagellum or the head. We validated the use of FM4-64 as an indicator for the occurrence of the AR by simultaneously detecting its fluorescence increase and the loss of EGFP in transgenic EGFPAcr sperm. For the first time, we have simultaneously visualized the rise in [Ca2+]i and the process of exocytosis in response to Progesterone and found that only a specific transitory increase in [Ca2+]i originated in the sperm head promotes the initiation of AR.
Fil: Romarowski, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Sánchez Cárdenas, Claudia. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Ramírez Gómez, Héctor V.. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Puga Molina, Lis del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Treviño, Claudia L.. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Hernández Cruz, Arturo. Universidad Nacional Autónoma de México. Instituto de Fisiología Celular; México
Fil: Darszon, Alberto I.. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
description During capacitation, sperm acquire the ability to undergo the acrosome reaction (AR), an essential step in fertilization. Progesterone produced by cumulus cells has been associated with various physiological processes in sperm, including stimulation of AR. An increase in intracellular Ca2+ ([Ca2+]i) is necessary for AR to occur. In this study, we investigated the spatio- temporal correlation between the changes in [Ca2+]i and AR in single mouse spermatozoa in response to Progesterone. We found that Progesterone stimulates an [Ca2+]i increase in five different patterns: gradual increase, oscillatory, late transitory, immediate transitory and sustained. We also observed that the [Ca2+]i increase promoted by Progesterone starts at either the flagellum or the head. We validated the use of FM4-64 as an indicator for the occurrence of the AR by simultaneously detecting its fluorescence increase and the loss of EGFP in transgenic EGFPAcr sperm. For the first time, we have simultaneously visualized the rise in [Ca2+]i and the process of exocytosis in response to Progesterone and found that only a specific transitory increase in [Ca2+]i originated in the sperm head promotes the initiation of AR.
publishDate 2016
dc.date.none.fl_str_mv 2016-03-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/23038
Romarowski, Ana; Sánchez Cárdenas, Claudia; Ramírez Gómez, Héctor V.; Puga Molina, Lis del Carmen; Treviño, Claudia L.; et al.; A specific transitory increase in intracellular calcium induced by progesterone promotes acrosomal exocytosis in mouse sperm; Society for the Study of Reproduction; Biology of Reproduction; 94; 3; 1-3-2016; 1-12
0006-3363
1529-7268
CONICET Digital
CONICET
url http://hdl.handle.net/11336/23038
identifier_str_mv Romarowski, Ana; Sánchez Cárdenas, Claudia; Ramírez Gómez, Héctor V.; Puga Molina, Lis del Carmen; Treviño, Claudia L.; et al.; A specific transitory increase in intracellular calcium induced by progesterone promotes acrosomal exocytosis in mouse sperm; Society for the Study of Reproduction; Biology of Reproduction; 94; 3; 1-3-2016; 1-12
0006-3363
1529-7268
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolreprod/article-lookup/doi/10.1095/biolreprod.115.136085
info:eu-repo/semantics/altIdentifier/doi/10.1095/biolreprod.115.136085
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4829090/
info:eu-repo/semantics/altIdentifier/pmid/26819478
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society for the Study of Reproduction
publisher.none.fl_str_mv Society for the Study of Reproduction
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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