Super-resolution imaging of live sperm reveals dynamic changes of the actin cytoskeleton during acrosomal exocytosis
- Autores
- Romarowski, Ana; Velasco Félix, Ángel G.; Torres Rodriguez, Paulina; Gervasi, María G.; Xu, Xinran; Luque, Guillermina Maria; Contreras Jiménez, Gastón; Sánchez Cárdenas, Claudia; Ramírez Gómez, Héctor V.; Krapf, Diego; Visconti, Pablo E.; Krapf, Dario; Guerrero, Adán; Darszon, Alberto; Buffone, Mariano Gabriel
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Filamentous actin (F-actin) is a key factor in exocytosis in many cell types. In mammalian sperm, acrosomal exocytosis (denoted the acrosome reaction or AR), a special type of controlled secretion, is regulated by multiple signaling pathways and the actin cytoskeleton. However, the dynamic changes of the actin cytoskeleton in live sperm are largely not understood. Here, we used the powerful properties of SiR-actin to examine actin dynamics in live mouse sperm at the onset of the AR. By using a combination of super-resolution microscopy techniques to image sperm loaded with SiR-actin or sperm from transgenic mice containing Lifeact-EGFP, six regions containing F-actin within the sperm head were revealed. The proportion of sperm possessing these structures changed upon capacitation. By performing live-cell imaging experiments, we report that dynamic changes of F-actin during the AR occur in specific regions of the sperm head. While certain F-actin regions undergo depolymerization prior to the initiation of the AR, others remain unaltered or are lost after exocytosis occurs. Our work emphasizes the utility of live-cell nanoscopy, which will undoubtedly impact the search for mechanisms that underlie basic sperm functions.
Fil: Romarowski, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Velasco Félix, Ángel G.. Universidad Nacional Autónoma de México; México
Fil: Rodriguez, Paulina Torres. Universidad Nacional Autónoma de México; México
Fil: Gervasi, Mar?á G.. University Of Massachusetts Amherst;
Fil: Xu, Xinran. School Of Biomedical Engineering;
Fil: Luque, Guillermina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Contreras-Jiménez, Gastón. Universidad Nacional Autónoma de México; México
Fil: Sánchez-Cárdenas, Claudia. Universidad Nacional Autónoma de México; México
Fil: Ramírez-Gómez, Héctor V.. Universidad Nacional Autónoma de México; México
Fil: Krapf, Diego. School Of Biomedical Engineering;
Fil: Visconti, Pablo E.. University Of Massachusetts Amherst;
Fil: Krapf, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Guerrero, Adán. Universidad Nacional Autónoma de México; México
Fil: Darszon, Alberto. Universidad Nacional Autónoma de México; México
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
ACROSOMAL EXOCYTOSIS
ACTIN
SPERM - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/93636
Ver los metadatos del registro completo
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Super-resolution imaging of live sperm reveals dynamic changes of the actin cytoskeleton during acrosomal exocytosisRomarowski, AnaVelasco Félix, Ángel G.Torres Rodriguez, PaulinaGervasi, María G.Xu, XinranLuque, Guillermina MariaContreras Jiménez, GastónSánchez Cárdenas, ClaudiaRamírez Gómez, Héctor V.Krapf, DiegoVisconti, Pablo E.Krapf, DarioGuerrero, AdánDarszon, AlbertoBuffone, Mariano GabrielACROSOMAL EXOCYTOSISACTINSPERMhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Filamentous actin (F-actin) is a key factor in exocytosis in many cell types. In mammalian sperm, acrosomal exocytosis (denoted the acrosome reaction or AR), a special type of controlled secretion, is regulated by multiple signaling pathways and the actin cytoskeleton. However, the dynamic changes of the actin cytoskeleton in live sperm are largely not understood. Here, we used the powerful properties of SiR-actin to examine actin dynamics in live mouse sperm at the onset of the AR. By using a combination of super-resolution microscopy techniques to image sperm loaded with SiR-actin or sperm from transgenic mice containing Lifeact-EGFP, six regions containing F-actin within the sperm head were revealed. The proportion of sperm possessing these structures changed upon capacitation. By performing live-cell imaging experiments, we report that dynamic changes of F-actin during the AR occur in specific regions of the sperm head. While certain F-actin regions undergo depolymerization prior to the initiation of the AR, others remain unaltered or are lost after exocytosis occurs. Our work emphasizes the utility of live-cell nanoscopy, which will undoubtedly impact the search for mechanisms that underlie basic sperm functions.Fil: Romarowski, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Velasco Félix, Ángel G.. Universidad Nacional Autónoma de México; MéxicoFil: Rodriguez, Paulina Torres. Universidad Nacional Autónoma de México; MéxicoFil: Gervasi, Mar?á G.. University Of Massachusetts Amherst;Fil: Xu, Xinran. School Of Biomedical Engineering;Fil: Luque, Guillermina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Contreras-Jiménez, Gastón. Universidad Nacional Autónoma de México; MéxicoFil: Sánchez-Cárdenas, Claudia. Universidad Nacional Autónoma de México; MéxicoFil: Ramírez-Gómez, Héctor V.. Universidad Nacional Autónoma de México; MéxicoFil: Krapf, Diego. School Of Biomedical Engineering;Fil: Visconti, Pablo E.. University Of Massachusetts Amherst;Fil: Krapf, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Guerrero, Adán. Universidad Nacional Autónoma de México; MéxicoFil: Darszon, Alberto. Universidad Nacional Autónoma de México; MéxicoFil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaCompany of Biologists2018-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/93636Romarowski, Ana; Velasco Félix, Ángel G.; Torres Rodriguez, Paulina; Gervasi, María G.; Xu, Xinran; et al.; Super-resolution imaging of live sperm reveals dynamic changes of the actin cytoskeleton during acrosomal exocytosis; Company of Biologists; Journal of Cell Science; 131; 21; 11-2018; 1-140021-9533CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1242/jcs.218958info:eu-repo/semantics/altIdentifier/url/https://jcs.biologists.org/content/131/21/jcs218958info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:42:43Zoai:ri.conicet.gov.ar:11336/93636instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:42:43.493CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Super-resolution imaging of live sperm reveals dynamic changes of the actin cytoskeleton during acrosomal exocytosis |
| title |
Super-resolution imaging of live sperm reveals dynamic changes of the actin cytoskeleton during acrosomal exocytosis |
| spellingShingle |
Super-resolution imaging of live sperm reveals dynamic changes of the actin cytoskeleton during acrosomal exocytosis Romarowski, Ana ACROSOMAL EXOCYTOSIS ACTIN SPERM |
| title_short |
Super-resolution imaging of live sperm reveals dynamic changes of the actin cytoskeleton during acrosomal exocytosis |
| title_full |
Super-resolution imaging of live sperm reveals dynamic changes of the actin cytoskeleton during acrosomal exocytosis |
| title_fullStr |
Super-resolution imaging of live sperm reveals dynamic changes of the actin cytoskeleton during acrosomal exocytosis |
| title_full_unstemmed |
Super-resolution imaging of live sperm reveals dynamic changes of the actin cytoskeleton during acrosomal exocytosis |
| title_sort |
Super-resolution imaging of live sperm reveals dynamic changes of the actin cytoskeleton during acrosomal exocytosis |
| dc.creator.none.fl_str_mv |
Romarowski, Ana Velasco Félix, Ángel G. Torres Rodriguez, Paulina Gervasi, María G. Xu, Xinran Luque, Guillermina Maria Contreras Jiménez, Gastón Sánchez Cárdenas, Claudia Ramírez Gómez, Héctor V. Krapf, Diego Visconti, Pablo E. Krapf, Dario Guerrero, Adán Darszon, Alberto Buffone, Mariano Gabriel |
| author |
Romarowski, Ana |
| author_facet |
Romarowski, Ana Velasco Félix, Ángel G. Torres Rodriguez, Paulina Gervasi, María G. Xu, Xinran Luque, Guillermina Maria Contreras Jiménez, Gastón Sánchez Cárdenas, Claudia Ramírez Gómez, Héctor V. Krapf, Diego Visconti, Pablo E. Krapf, Dario Guerrero, Adán Darszon, Alberto Buffone, Mariano Gabriel |
| author_role |
author |
| author2 |
Velasco Félix, Ángel G. Torres Rodriguez, Paulina Gervasi, María G. Xu, Xinran Luque, Guillermina Maria Contreras Jiménez, Gastón Sánchez Cárdenas, Claudia Ramírez Gómez, Héctor V. Krapf, Diego Visconti, Pablo E. Krapf, Dario Guerrero, Adán Darszon, Alberto Buffone, Mariano Gabriel |
| author2_role |
author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
ACROSOMAL EXOCYTOSIS ACTIN SPERM |
| topic |
ACROSOMAL EXOCYTOSIS ACTIN SPERM |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
Filamentous actin (F-actin) is a key factor in exocytosis in many cell types. In mammalian sperm, acrosomal exocytosis (denoted the acrosome reaction or AR), a special type of controlled secretion, is regulated by multiple signaling pathways and the actin cytoskeleton. However, the dynamic changes of the actin cytoskeleton in live sperm are largely not understood. Here, we used the powerful properties of SiR-actin to examine actin dynamics in live mouse sperm at the onset of the AR. By using a combination of super-resolution microscopy techniques to image sperm loaded with SiR-actin or sperm from transgenic mice containing Lifeact-EGFP, six regions containing F-actin within the sperm head were revealed. The proportion of sperm possessing these structures changed upon capacitation. By performing live-cell imaging experiments, we report that dynamic changes of F-actin during the AR occur in specific regions of the sperm head. While certain F-actin regions undergo depolymerization prior to the initiation of the AR, others remain unaltered or are lost after exocytosis occurs. Our work emphasizes the utility of live-cell nanoscopy, which will undoubtedly impact the search for mechanisms that underlie basic sperm functions. Fil: Romarowski, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Velasco Félix, Ángel G.. Universidad Nacional Autónoma de México; México Fil: Rodriguez, Paulina Torres. Universidad Nacional Autónoma de México; México Fil: Gervasi, Mar?á G.. University Of Massachusetts Amherst; Fil: Xu, Xinran. School Of Biomedical Engineering; Fil: Luque, Guillermina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Contreras-Jiménez, Gastón. Universidad Nacional Autónoma de México; México Fil: Sánchez-Cárdenas, Claudia. Universidad Nacional Autónoma de México; México Fil: Ramírez-Gómez, Héctor V.. Universidad Nacional Autónoma de México; México Fil: Krapf, Diego. School Of Biomedical Engineering; Fil: Visconti, Pablo E.. University Of Massachusetts Amherst; Fil: Krapf, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina Fil: Guerrero, Adán. Universidad Nacional Autónoma de México; México Fil: Darszon, Alberto. Universidad Nacional Autónoma de México; México Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
| description |
Filamentous actin (F-actin) is a key factor in exocytosis in many cell types. In mammalian sperm, acrosomal exocytosis (denoted the acrosome reaction or AR), a special type of controlled secretion, is regulated by multiple signaling pathways and the actin cytoskeleton. However, the dynamic changes of the actin cytoskeleton in live sperm are largely not understood. Here, we used the powerful properties of SiR-actin to examine actin dynamics in live mouse sperm at the onset of the AR. By using a combination of super-resolution microscopy techniques to image sperm loaded with SiR-actin or sperm from transgenic mice containing Lifeact-EGFP, six regions containing F-actin within the sperm head were revealed. The proportion of sperm possessing these structures changed upon capacitation. By performing live-cell imaging experiments, we report that dynamic changes of F-actin during the AR occur in specific regions of the sperm head. While certain F-actin regions undergo depolymerization prior to the initiation of the AR, others remain unaltered or are lost after exocytosis occurs. Our work emphasizes the utility of live-cell nanoscopy, which will undoubtedly impact the search for mechanisms that underlie basic sperm functions. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018-11 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/93636 Romarowski, Ana; Velasco Félix, Ángel G.; Torres Rodriguez, Paulina; Gervasi, María G.; Xu, Xinran; et al.; Super-resolution imaging of live sperm reveals dynamic changes of the actin cytoskeleton during acrosomal exocytosis; Company of Biologists; Journal of Cell Science; 131; 21; 11-2018; 1-14 0021-9533 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/93636 |
| identifier_str_mv |
Romarowski, Ana; Velasco Félix, Ángel G.; Torres Rodriguez, Paulina; Gervasi, María G.; Xu, Xinran; et al.; Super-resolution imaging of live sperm reveals dynamic changes of the actin cytoskeleton during acrosomal exocytosis; Company of Biologists; Journal of Cell Science; 131; 21; 11-2018; 1-14 0021-9533 CONICET Digital CONICET |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1242/jcs.218958 info:eu-repo/semantics/altIdentifier/url/https://jcs.biologists.org/content/131/21/jcs218958 |
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openAccess |
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application/pdf application/pdf application/pdf application/pdf |
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Company of Biologists |
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Company of Biologists |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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