Unresolved questions concerning mammalian sperm acrosomal exocytosis
- Autores
- Buffone, Mariano Gabriel; Hirohashi, Noritaka; Gerton, George L.
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In recent years, the study of mammalian acrosomal exocytosis has produced some major advances that challenge the long-held, general paradigms in the field. Principally, the idea that sperm must be acrosome-intact to bind to the zona pellucida of unfertilized eggs, based largely on in vitro fertilization studies of mouse oocytes denuded of the cumulus oophorus, has been overturned by experiments using state-of-the-art imaging of cumulus-intact oocytes and fertilization experiments where eggs were reinseminated by acrosome-reacted sperm recovered from the perivitelline space of zygotes. In light of these results, this minireview highlights a number of unresolved questions and emphasizes the fact that there is still much work to be done in this exciting field. Future experiments using recently advanced technologies should lead to a more complete and accurate understanding of the molecular mechanisms governing the fertilization process in mammals.
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Hirohashi, Noritaka. Shimane University. Education and Research Center for Biological Resources; Japón
Fil: Gerton, George L.. University Of Pennsylvania; Estados Unidos - Materia
-
Acrosomal Exocytosis
Fertilization
Capacitation
Spermatozoa - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/7664
Ver los metadatos del registro completo
id |
CONICETDig_29dc624afd338287c2081d6f57bcd6d9 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/7664 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Unresolved questions concerning mammalian sperm acrosomal exocytosisBuffone, Mariano GabrielHirohashi, NoritakaGerton, George L.Acrosomal ExocytosisFertilizationCapacitationSpermatozoahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3In recent years, the study of mammalian acrosomal exocytosis has produced some major advances that challenge the long-held, general paradigms in the field. Principally, the idea that sperm must be acrosome-intact to bind to the zona pellucida of unfertilized eggs, based largely on in vitro fertilization studies of mouse oocytes denuded of the cumulus oophorus, has been overturned by experiments using state-of-the-art imaging of cumulus-intact oocytes and fertilization experiments where eggs were reinseminated by acrosome-reacted sperm recovered from the perivitelline space of zygotes. In light of these results, this minireview highlights a number of unresolved questions and emphasizes the fact that there is still much work to be done in this exciting field. Future experiments using recently advanced technologies should lead to a more complete and accurate understanding of the molecular mechanisms governing the fertilization process in mammals.Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Hirohashi, Noritaka. Shimane University. Education and Research Center for Biological Resources; JapónFil: Gerton, George L.. University Of Pennsylvania; Estados UnidosSoc Study Reproduction2014-03-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7664Buffone, Mariano Gabriel; Hirohashi, Noritaka; Gerton, George L.; Unresolved questions concerning mammalian sperm acrosomal exocytosis; Soc Study Reproduction; Biology Of Reproduction; 90; 5; 26-3-2014; 112-1160006-33631529-7268enginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolreprod/article/90/5/112,%201-8/2514360info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:43Zoai:ri.conicet.gov.ar:11336/7664instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:44.05CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Unresolved questions concerning mammalian sperm acrosomal exocytosis |
title |
Unresolved questions concerning mammalian sperm acrosomal exocytosis |
spellingShingle |
Unresolved questions concerning mammalian sperm acrosomal exocytosis Buffone, Mariano Gabriel Acrosomal Exocytosis Fertilization Capacitation Spermatozoa |
title_short |
Unresolved questions concerning mammalian sperm acrosomal exocytosis |
title_full |
Unresolved questions concerning mammalian sperm acrosomal exocytosis |
title_fullStr |
Unresolved questions concerning mammalian sperm acrosomal exocytosis |
title_full_unstemmed |
Unresolved questions concerning mammalian sperm acrosomal exocytosis |
title_sort |
Unresolved questions concerning mammalian sperm acrosomal exocytosis |
dc.creator.none.fl_str_mv |
Buffone, Mariano Gabriel Hirohashi, Noritaka Gerton, George L. |
author |
Buffone, Mariano Gabriel |
author_facet |
Buffone, Mariano Gabriel Hirohashi, Noritaka Gerton, George L. |
author_role |
author |
author2 |
Hirohashi, Noritaka Gerton, George L. |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Acrosomal Exocytosis Fertilization Capacitation Spermatozoa |
topic |
Acrosomal Exocytosis Fertilization Capacitation Spermatozoa |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
In recent years, the study of mammalian acrosomal exocytosis has produced some major advances that challenge the long-held, general paradigms in the field. Principally, the idea that sperm must be acrosome-intact to bind to the zona pellucida of unfertilized eggs, based largely on in vitro fertilization studies of mouse oocytes denuded of the cumulus oophorus, has been overturned by experiments using state-of-the-art imaging of cumulus-intact oocytes and fertilization experiments where eggs were reinseminated by acrosome-reacted sperm recovered from the perivitelline space of zygotes. In light of these results, this minireview highlights a number of unresolved questions and emphasizes the fact that there is still much work to be done in this exciting field. Future experiments using recently advanced technologies should lead to a more complete and accurate understanding of the molecular mechanisms governing the fertilization process in mammals. Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina Fil: Hirohashi, Noritaka. Shimane University. Education and Research Center for Biological Resources; Japón Fil: Gerton, George L.. University Of Pennsylvania; Estados Unidos |
description |
In recent years, the study of mammalian acrosomal exocytosis has produced some major advances that challenge the long-held, general paradigms in the field. Principally, the idea that sperm must be acrosome-intact to bind to the zona pellucida of unfertilized eggs, based largely on in vitro fertilization studies of mouse oocytes denuded of the cumulus oophorus, has been overturned by experiments using state-of-the-art imaging of cumulus-intact oocytes and fertilization experiments where eggs were reinseminated by acrosome-reacted sperm recovered from the perivitelline space of zygotes. In light of these results, this minireview highlights a number of unresolved questions and emphasizes the fact that there is still much work to be done in this exciting field. Future experiments using recently advanced technologies should lead to a more complete and accurate understanding of the molecular mechanisms governing the fertilization process in mammals. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-03-26 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/7664 Buffone, Mariano Gabriel; Hirohashi, Noritaka; Gerton, George L.; Unresolved questions concerning mammalian sperm acrosomal exocytosis; Soc Study Reproduction; Biology Of Reproduction; 90; 5; 26-3-2014; 112-116 0006-3363 1529-7268 |
url |
http://hdl.handle.net/11336/7664 |
identifier_str_mv |
Buffone, Mariano Gabriel; Hirohashi, Noritaka; Gerton, George L.; Unresolved questions concerning mammalian sperm acrosomal exocytosis; Soc Study Reproduction; Biology Of Reproduction; 90; 5; 26-3-2014; 112-116 0006-3363 1529-7268 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolreprod/article/90/5/112,%201-8/2514360 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Soc Study Reproduction |
publisher.none.fl_str_mv |
Soc Study Reproduction |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1842269655610163200 |
score |
13.13397 |