Unresolved questions concerning mammalian sperm acrosomal exocytosis

Autores
Buffone, Mariano Gabriel; Hirohashi, Noritaka; Gerton, George L.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In recent years, the study of mammalian acrosomal exocytosis has produced some major advances that challenge the long-held, general paradigms in the field. Principally, the idea that sperm must be acrosome-intact to bind to the zona pellucida of unfertilized eggs, based largely on in vitro fertilization studies of mouse oocytes denuded of the cumulus oophorus, has been overturned by experiments using state-of-the-art imaging of cumulus-intact oocytes and fertilization experiments where eggs were reinseminated by acrosome-reacted sperm recovered from the perivitelline space of zygotes. In light of these results, this minireview highlights a number of unresolved questions and emphasizes the fact that there is still much work to be done in this exciting field. Future experiments using recently advanced technologies should lead to a more complete and accurate understanding of the molecular mechanisms governing the fertilization process in mammals.
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Hirohashi, Noritaka. Shimane University. Education and Research Center for Biological Resources; Japón
Fil: Gerton, George L.. University Of Pennsylvania; Estados Unidos
Materia
Acrosomal Exocytosis
Fertilization
Capacitation
Spermatozoa
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7664

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network_name_str CONICET Digital (CONICET)
spelling Unresolved questions concerning mammalian sperm acrosomal exocytosisBuffone, Mariano GabrielHirohashi, NoritakaGerton, George L.Acrosomal ExocytosisFertilizationCapacitationSpermatozoahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3In recent years, the study of mammalian acrosomal exocytosis has produced some major advances that challenge the long-held, general paradigms in the field. Principally, the idea that sperm must be acrosome-intact to bind to the zona pellucida of unfertilized eggs, based largely on in vitro fertilization studies of mouse oocytes denuded of the cumulus oophorus, has been overturned by experiments using state-of-the-art imaging of cumulus-intact oocytes and fertilization experiments where eggs were reinseminated by acrosome-reacted sperm recovered from the perivitelline space of zygotes. In light of these results, this minireview highlights a number of unresolved questions and emphasizes the fact that there is still much work to be done in this exciting field. Future experiments using recently advanced technologies should lead to a more complete and accurate understanding of the molecular mechanisms governing the fertilization process in mammals.Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Hirohashi, Noritaka. Shimane University. Education and Research Center for Biological Resources; JapónFil: Gerton, George L.. University Of Pennsylvania; Estados UnidosSoc Study Reproduction2014-03-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7664Buffone, Mariano Gabriel; Hirohashi, Noritaka; Gerton, George L.; Unresolved questions concerning mammalian sperm acrosomal exocytosis; Soc Study Reproduction; Biology Of Reproduction; 90; 5; 26-3-2014; 112-1160006-33631529-7268enginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolreprod/article/90/5/112,%201-8/2514360info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:43Zoai:ri.conicet.gov.ar:11336/7664instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:44.05CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Unresolved questions concerning mammalian sperm acrosomal exocytosis
title Unresolved questions concerning mammalian sperm acrosomal exocytosis
spellingShingle Unresolved questions concerning mammalian sperm acrosomal exocytosis
Buffone, Mariano Gabriel
Acrosomal Exocytosis
Fertilization
Capacitation
Spermatozoa
title_short Unresolved questions concerning mammalian sperm acrosomal exocytosis
title_full Unresolved questions concerning mammalian sperm acrosomal exocytosis
title_fullStr Unresolved questions concerning mammalian sperm acrosomal exocytosis
title_full_unstemmed Unresolved questions concerning mammalian sperm acrosomal exocytosis
title_sort Unresolved questions concerning mammalian sperm acrosomal exocytosis
dc.creator.none.fl_str_mv Buffone, Mariano Gabriel
Hirohashi, Noritaka
Gerton, George L.
author Buffone, Mariano Gabriel
author_facet Buffone, Mariano Gabriel
Hirohashi, Noritaka
Gerton, George L.
author_role author
author2 Hirohashi, Noritaka
Gerton, George L.
author2_role author
author
dc.subject.none.fl_str_mv Acrosomal Exocytosis
Fertilization
Capacitation
Spermatozoa
topic Acrosomal Exocytosis
Fertilization
Capacitation
Spermatozoa
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In recent years, the study of mammalian acrosomal exocytosis has produced some major advances that challenge the long-held, general paradigms in the field. Principally, the idea that sperm must be acrosome-intact to bind to the zona pellucida of unfertilized eggs, based largely on in vitro fertilization studies of mouse oocytes denuded of the cumulus oophorus, has been overturned by experiments using state-of-the-art imaging of cumulus-intact oocytes and fertilization experiments where eggs were reinseminated by acrosome-reacted sperm recovered from the perivitelline space of zygotes. In light of these results, this minireview highlights a number of unresolved questions and emphasizes the fact that there is still much work to be done in this exciting field. Future experiments using recently advanced technologies should lead to a more complete and accurate understanding of the molecular mechanisms governing the fertilization process in mammals.
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Hirohashi, Noritaka. Shimane University. Education and Research Center for Biological Resources; Japón
Fil: Gerton, George L.. University Of Pennsylvania; Estados Unidos
description In recent years, the study of mammalian acrosomal exocytosis has produced some major advances that challenge the long-held, general paradigms in the field. Principally, the idea that sperm must be acrosome-intact to bind to the zona pellucida of unfertilized eggs, based largely on in vitro fertilization studies of mouse oocytes denuded of the cumulus oophorus, has been overturned by experiments using state-of-the-art imaging of cumulus-intact oocytes and fertilization experiments where eggs were reinseminated by acrosome-reacted sperm recovered from the perivitelline space of zygotes. In light of these results, this minireview highlights a number of unresolved questions and emphasizes the fact that there is still much work to be done in this exciting field. Future experiments using recently advanced technologies should lead to a more complete and accurate understanding of the molecular mechanisms governing the fertilization process in mammals.
publishDate 2014
dc.date.none.fl_str_mv 2014-03-26
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7664
Buffone, Mariano Gabriel; Hirohashi, Noritaka; Gerton, George L.; Unresolved questions concerning mammalian sperm acrosomal exocytosis; Soc Study Reproduction; Biology Of Reproduction; 90; 5; 26-3-2014; 112-116
0006-3363
1529-7268
url http://hdl.handle.net/11336/7664
identifier_str_mv Buffone, Mariano Gabriel; Hirohashi, Noritaka; Gerton, George L.; Unresolved questions concerning mammalian sperm acrosomal exocytosis; Soc Study Reproduction; Biology Of Reproduction; 90; 5; 26-3-2014; 112-116
0006-3363
1529-7268
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolreprod/article/90/5/112,%201-8/2514360
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Soc Study Reproduction
publisher.none.fl_str_mv Soc Study Reproduction
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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