Serotonin (5-HT) and catecholamines (CA) antagonistic food-related behaviors in C. elegans

Autores
Blanco, Maria Gabriela; de Rosa, Maria Jose; Rayes, Diego Hernán
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Despite the intermodulation between serotonergic and adrenergic signals is crucial throughout the animal kingdom, the molecular and cellular mechanisms underlying this interrelation are poorly understood. We here use C. elegans as a model to get insights into the neural circuits linking 5-TH and CA. When food-deprived worms encounter food, 5-HT is released to slow-down their locomotion and to stimulate pharyngeal pumping.In contrast, exogenous Tyramine (TA) and Octopamine (OA), invertebrate counterparts for adrenaline and noradrenaline, stimulate locomotion and decreases pharyngeal pumping. We found that tdc-1 mutants (unable to synthesize TA and OA) are hypersensitive to 5-HT-mediated paralysis, suggesting that TA and OA acts antagonistically to 5-HT. We also identify the TA (TYRA-3) and OA (SER-3 and SER-6) receptors involved in this antagonism. Moreover, our calcium imaging recordings showed that the peak of activity of serotoninergic neurons upon encountering food is significantly higher in tdc-1 null mutant background. Taken together these results suggest that TA and OA counteract serotoninergic signaling by driving opposing behaviors and by inhibiting 5-HT release. Our final aim is to decipher the neural circuit and the molecules involved in the reciprocal modulation between CA and 5-HT in C. elegans. Given the conservation in molecular components of these pathways, our studies are likely significant to understand this interrelation in other animals.
Fil: Blanco, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Rayes, Diego Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
XXXIV Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias (SAN)
Villa Carlos Paz
Argentina
Sociedad Argentina de Investigación en Neurociencias
Materia
BIOGENIC AMINES
ANTAGONISM
NEURONAL ACTIVITY
FOOD
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/192244
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Serotonin (5-HT) and catecholamines (CA) antagonistic food-related behaviors in C. elegansBlanco, Maria Gabrielade Rosa, Maria JoseRayes, Diego HernánBIOGENIC AMINESANTAGONISMNEURONAL ACTIVITYFOODhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Despite the intermodulation between serotonergic and adrenergic signals is crucial throughout the animal kingdom, the molecular and cellular mechanisms underlying this interrelation are poorly understood. We here use C. elegans as a model to get insights into the neural circuits linking 5-TH and CA. When food-deprived worms encounter food, 5-HT is released to slow-down their locomotion and to stimulate pharyngeal pumping.In contrast, exogenous Tyramine (TA) and Octopamine (OA), invertebrate counterparts for adrenaline and noradrenaline, stimulate locomotion and decreases pharyngeal pumping. We found that tdc-1 mutants (unable to synthesize TA and OA) are hypersensitive to 5-HT-mediated paralysis, suggesting that TA and OA acts antagonistically to 5-HT. We also identify the TA (TYRA-3) and OA (SER-3 and SER-6) receptors involved in this antagonism. Moreover, our calcium imaging recordings showed that the peak of activity of serotoninergic neurons upon encountering food is significantly higher in tdc-1 null mutant background. Taken together these results suggest that TA and OA counteract serotoninergic signaling by driving opposing behaviors and by inhibiting 5-HT release. Our final aim is to decipher the neural circuit and the molecules involved in the reciprocal modulation between CA and 5-HT in C. elegans. Given the conservation in molecular components of these pathways, our studies are likely significant to understand this interrelation in other animals.Fil: Blanco, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Rayes, Diego Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaXXXIV Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias (SAN)Villa Carlos PazArgentinaSociedad Argentina de Investigación en NeurocienciasSociedad Argentina de Investigación en Neurociencias2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/192244Serotonin (5-HT) and catecholamines (CA) antagonistic food-related behaviors in C. elegans; XXXIV Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias (SAN); Villa Carlos Paz; Argentina; 2019; 43-43CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saneurociencias.org.ar/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-06-04T11:08:53Zoai:ri.conicet.gov.ar:11336/192244instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-06-04 11:08:53.735CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Serotonin (5-HT) and catecholamines (CA) antagonistic food-related behaviors in C. elegans
title Serotonin (5-HT) and catecholamines (CA) antagonistic food-related behaviors in C. elegans
spellingShingle Serotonin (5-HT) and catecholamines (CA) antagonistic food-related behaviors in C. elegans
Blanco, Maria Gabriela
BIOGENIC AMINES
ANTAGONISM
NEURONAL ACTIVITY
FOOD
title_short Serotonin (5-HT) and catecholamines (CA) antagonistic food-related behaviors in C. elegans
title_full Serotonin (5-HT) and catecholamines (CA) antagonistic food-related behaviors in C. elegans
title_fullStr Serotonin (5-HT) and catecholamines (CA) antagonistic food-related behaviors in C. elegans
title_full_unstemmed Serotonin (5-HT) and catecholamines (CA) antagonistic food-related behaviors in C. elegans
title_sort Serotonin (5-HT) and catecholamines (CA) antagonistic food-related behaviors in C. elegans
dc.creator.none.fl_str_mv Blanco, Maria Gabriela
de Rosa, Maria Jose
Rayes, Diego Hernán
author Blanco, Maria Gabriela
author_facet Blanco, Maria Gabriela
de Rosa, Maria Jose
Rayes, Diego Hernán
author_role author
author2 de Rosa, Maria Jose
Rayes, Diego Hernán
author2_role author
author
dc.subject.none.fl_str_mv BIOGENIC AMINES
ANTAGONISM
NEURONAL ACTIVITY
FOOD
topic BIOGENIC AMINES
ANTAGONISM
NEURONAL ACTIVITY
FOOD
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Despite the intermodulation between serotonergic and adrenergic signals is crucial throughout the animal kingdom, the molecular and cellular mechanisms underlying this interrelation are poorly understood. We here use C. elegans as a model to get insights into the neural circuits linking 5-TH and CA. When food-deprived worms encounter food, 5-HT is released to slow-down their locomotion and to stimulate pharyngeal pumping.In contrast, exogenous Tyramine (TA) and Octopamine (OA), invertebrate counterparts for adrenaline and noradrenaline, stimulate locomotion and decreases pharyngeal pumping. We found that tdc-1 mutants (unable to synthesize TA and OA) are hypersensitive to 5-HT-mediated paralysis, suggesting that TA and OA acts antagonistically to 5-HT. We also identify the TA (TYRA-3) and OA (SER-3 and SER-6) receptors involved in this antagonism. Moreover, our calcium imaging recordings showed that the peak of activity of serotoninergic neurons upon encountering food is significantly higher in tdc-1 null mutant background. Taken together these results suggest that TA and OA counteract serotoninergic signaling by driving opposing behaviors and by inhibiting 5-HT release. Our final aim is to decipher the neural circuit and the molecules involved in the reciprocal modulation between CA and 5-HT in C. elegans. Given the conservation in molecular components of these pathways, our studies are likely significant to understand this interrelation in other animals.
Fil: Blanco, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Rayes, Diego Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
XXXIV Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias (SAN)
Villa Carlos Paz
Argentina
Sociedad Argentina de Investigación en Neurociencias
description Despite the intermodulation between serotonergic and adrenergic signals is crucial throughout the animal kingdom, the molecular and cellular mechanisms underlying this interrelation are poorly understood. We here use C. elegans as a model to get insights into the neural circuits linking 5-TH and CA. When food-deprived worms encounter food, 5-HT is released to slow-down their locomotion and to stimulate pharyngeal pumping.In contrast, exogenous Tyramine (TA) and Octopamine (OA), invertebrate counterparts for adrenaline and noradrenaline, stimulate locomotion and decreases pharyngeal pumping. We found that tdc-1 mutants (unable to synthesize TA and OA) are hypersensitive to 5-HT-mediated paralysis, suggesting that TA and OA acts antagonistically to 5-HT. We also identify the TA (TYRA-3) and OA (SER-3 and SER-6) receptors involved in this antagonism. Moreover, our calcium imaging recordings showed that the peak of activity of serotoninergic neurons upon encountering food is significantly higher in tdc-1 null mutant background. Taken together these results suggest that TA and OA counteract serotoninergic signaling by driving opposing behaviors and by inhibiting 5-HT release. Our final aim is to decipher the neural circuit and the molecules involved in the reciprocal modulation between CA and 5-HT in C. elegans. Given the conservation in molecular components of these pathways, our studies are likely significant to understand this interrelation in other animals.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
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Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/192244
Serotonin (5-HT) and catecholamines (CA) antagonistic food-related behaviors in C. elegans; XXXIV Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias (SAN); Villa Carlos Paz; Argentina; 2019; 43-43
CONICET Digital
CONICET
url http://hdl.handle.net/11336/192244
identifier_str_mv Serotonin (5-HT) and catecholamines (CA) antagonistic food-related behaviors in C. elegans; XXXIV Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias (SAN); Villa Carlos Paz; Argentina; 2019; 43-43
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/pdf
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application/pdf
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencias
publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencias
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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