Selection of indigenous lactic acid bacteria to reinforce the intestinal microbiota of newly hatched chicken – relevance of in vitro and ex vivo methods for strains characterizatio...

Autores: Babot, Jaime Daniel; Argañaraz Martínez, Fernando Eloy; Saavedra, Maria Lucila; Apella, Maria Cristina; Perez Chaia, Adriana Beatriz
Año de publicación: 2014
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: Based on the natural beneﬁts of the indigenous microbiota, lactic acid bacteria (LAB) from poultry origin were isolated from hens and broilers intestine, and their probiotic potential was further studied. The tol erance to digestion, adhesion, capture of a mannose-binding lectin, absence of virulent factors and antibiotic resistances were studied. Different in vitro and ex vivo assays were performed to select tolerant and adherent strains because standardized protocols have not been deﬁned. Fourteen strains highly tolerant to gastrointestinal digestion were genetically identiﬁed. Hydrophobic surfaces were not required for the bacterial adhesion and only nine strains adhered ex vivo to the intestinal mucosa. Three strains captured a lectin of the same speciﬁcity of Type-1 ﬁmbriae. Virulence factors were absent but some strains evidenced multiple antibiotic resistances. These results provide bases for a future standardization of methods for the selection of probiotic strains intended to reinforce the microbiota of newly hatched chickens.
Fil: Babot, Jaime Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Argañaraz Martínez, Fernando Eloy. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
Fil: Saavedra, Maria Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Apella, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Perez Chaia, Adriana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Materia: Poultry
Lactic Acid Bacteria
Probiotic
Safety
Selection Protocols
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/25509

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spelling	Selection of indigenous lactic acid bacteria to reinforce the intestinal microbiota of newly hatched chicken – relevance of in vitro and ex vivo methods for strains characterizationBabot, Jaime DanielArgañaraz Martínez, Fernando EloySaavedra, Maria LucilaApella, Maria CristinaPerez Chaia, Adriana BeatrizPoultryLactic Acid BacteriaProbioticSafetySelection Protocolshttps://purl.org/becyt/ford/4.2https://purl.org/becyt/ford/4Based on the natural beneﬁts of the indigenous microbiota, lactic acid bacteria (LAB) from poultry origin were isolated from hens and broilers intestine, and their probiotic potential was further studied. The tol erance to digestion, adhesion, capture of a mannose-binding lectin, absence of virulent factors and antibiotic resistances were studied. Different in vitro and ex vivo assays were performed to select tolerant and adherent strains because standardized protocols have not been deﬁned. Fourteen strains highly tolerant to gastrointestinal digestion were genetically identiﬁed. Hydrophobic surfaces were not required for the bacterial adhesion and only nine strains adhered ex vivo to the intestinal mucosa. Three strains captured a lectin of the same speciﬁcity of Type-1 ﬁmbriae. Virulence factors were absent but some strains evidenced multiple antibiotic resistances. These results provide bases for a future standardization of methods for the selection of probiotic strains intended to reinforce the microbiota of newly hatched chickens.Fil: Babot, Jaime Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Argañaraz Martínez, Fernando Eloy. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; ArgentinaFil: Saavedra, Maria Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Apella, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Perez Chaia, Adriana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaElsevier2014-06-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25509Babot, Jaime Daniel; Argañaraz Martínez, Fernando Eloy; Saavedra, Maria Lucila; Apella, Maria Cristina; Perez Chaia, Adriana Beatriz; Selection of indigenous lactic acid bacteria to reinforce the intestinal microbiota of newly hatched chicken – relevance of in vitro and ex vivo methods for strains characterization; Elsevier; Research in Veterinary Science; 97; 1; 13-6-2014; 8-170034-52881532-2661CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1016/j.rvsc.2014.06.001info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0034528814001635info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:18:07Zoai:ri.conicet.gov.ar:11336/25509instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:18:08.027CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Selection of indigenous lactic acid bacteria to reinforce the intestinal microbiota of newly hatched chicken – relevance of in vitro and ex vivo methods for strains characterization
title	Selection of indigenous lactic acid bacteria to reinforce the intestinal microbiota of newly hatched chicken – relevance of in vitro and ex vivo methods for strains characterization
spellingShingle	Selection of indigenous lactic acid bacteria to reinforce the intestinal microbiota of newly hatched chicken – relevance of in vitro and ex vivo methods for strains characterization Babot, Jaime Daniel Poultry Lactic Acid Bacteria Probiotic Safety Selection Protocols
title_short	Selection of indigenous lactic acid bacteria to reinforce the intestinal microbiota of newly hatched chicken – relevance of in vitro and ex vivo methods for strains characterization
title_full	Selection of indigenous lactic acid bacteria to reinforce the intestinal microbiota of newly hatched chicken – relevance of in vitro and ex vivo methods for strains characterization
title_fullStr	Selection of indigenous lactic acid bacteria to reinforce the intestinal microbiota of newly hatched chicken – relevance of in vitro and ex vivo methods for strains characterization
title_full_unstemmed	Selection of indigenous lactic acid bacteria to reinforce the intestinal microbiota of newly hatched chicken – relevance of in vitro and ex vivo methods for strains characterization
title_sort	Selection of indigenous lactic acid bacteria to reinforce the intestinal microbiota of newly hatched chicken – relevance of in vitro and ex vivo methods for strains characterization
dc.creator.none.fl_str_mv	Babot, Jaime Daniel Argañaraz Martínez, Fernando Eloy Saavedra, Maria Lucila Apella, Maria Cristina Perez Chaia, Adriana Beatriz
author	Babot, Jaime Daniel
author_facet	Babot, Jaime Daniel Argañaraz Martínez, Fernando Eloy Saavedra, Maria Lucila Apella, Maria Cristina Perez Chaia, Adriana Beatriz
author_role	author
author2	Argañaraz Martínez, Fernando Eloy Saavedra, Maria Lucila Apella, Maria Cristina Perez Chaia, Adriana Beatriz
author2_role	author author author author
dc.subject.none.fl_str_mv	Poultry Lactic Acid Bacteria Probiotic Safety Selection Protocols
topic	Poultry Lactic Acid Bacteria Probiotic Safety Selection Protocols
purl_subject.fl_str_mv	https://purl.org/becyt/ford/4.2 https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv	Based on the natural beneﬁts of the indigenous microbiota, lactic acid bacteria (LAB) from poultry origin were isolated from hens and broilers intestine, and their probiotic potential was further studied. The tol erance to digestion, adhesion, capture of a mannose-binding lectin, absence of virulent factors and antibiotic resistances were studied. Different in vitro and ex vivo assays were performed to select tolerant and adherent strains because standardized protocols have not been deﬁned. Fourteen strains highly tolerant to gastrointestinal digestion were genetically identiﬁed. Hydrophobic surfaces were not required for the bacterial adhesion and only nine strains adhered ex vivo to the intestinal mucosa. Three strains captured a lectin of the same speciﬁcity of Type-1 ﬁmbriae. Virulence factors were absent but some strains evidenced multiple antibiotic resistances. These results provide bases for a future standardization of methods for the selection of probiotic strains intended to reinforce the microbiota of newly hatched chickens. Fil: Babot, Jaime Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Argañaraz Martínez, Fernando Eloy. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina Fil: Saavedra, Maria Lucila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Apella, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina Fil: Perez Chaia, Adriana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
description	Based on the natural beneﬁts of the indigenous microbiota, lactic acid bacteria (LAB) from poultry origin were isolated from hens and broilers intestine, and their probiotic potential was further studied. The tol erance to digestion, adhesion, capture of a mannose-binding lectin, absence of virulent factors and antibiotic resistances were studied. Different in vitro and ex vivo assays were performed to select tolerant and adherent strains because standardized protocols have not been deﬁned. Fourteen strains highly tolerant to gastrointestinal digestion were genetically identiﬁed. Hydrophobic surfaces were not required for the bacterial adhesion and only nine strains adhered ex vivo to the intestinal mucosa. Three strains captured a lectin of the same speciﬁcity of Type-1 ﬁmbriae. Virulence factors were absent but some strains evidenced multiple antibiotic resistances. These results provide bases for a future standardization of methods for the selection of probiotic strains intended to reinforce the microbiota of newly hatched chickens.
publishDate	2014
dc.date.none.fl_str_mv	2014-06-13
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/25509 Babot, Jaime Daniel; Argañaraz Martínez, Fernando Eloy; Saavedra, Maria Lucila; Apella, Maria Cristina; Perez Chaia, Adriana Beatriz; Selection of indigenous lactic acid bacteria to reinforce the intestinal microbiota of newly hatched chicken – relevance of in vitro and ex vivo methods for strains characterization; Elsevier; Research in Veterinary Science; 97; 1; 13-6-2014; 8-17 0034-5288 1532-2661 CONICET Digital CONICET
url	http://hdl.handle.net/11336/25509
identifier_str_mv	Babot, Jaime Daniel; Argañaraz Martínez, Fernando Eloy; Saavedra, Maria Lucila; Apella, Maria Cristina; Perez Chaia, Adriana Beatriz; Selection of indigenous lactic acid bacteria to reinforce the intestinal microbiota of newly hatched chicken – relevance of in vitro and ex vivo methods for strains characterization; Elsevier; Research in Veterinary Science; 97; 1; 13-6-2014; 8-17 0034-5288 1532-2661 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1016/j.rvsc.2014.06.001 info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0034528814001635
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv	application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf
dc.publisher.none.fl_str_mv	Elsevier
publisher.none.fl_str_mv	Elsevier
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str	CONICET Digital (CONICET)
collection	CONICET Digital (CONICET)
instname_str	Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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Selection of indigenous lactic acid bacteria to reinforce the intestinal microbiota of newly hatched chicken – relevance of in vitro and ex vivo methods for strains characterizatio...

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