Safety and potential beneficial properties of Enterococcus strains isolated from kefir

Autores
Carasi, Paula; Jacquot, Claudine; Romanin, David Emmanuel; Elie, Anne Marie; de Antoni, Graciela Liliana; Urdaci, María C.; Serradell, María de los Ángeles
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Kefir is a fermented milk containing microorganisms showing several properties beneficial to health. In the present work the safety aspects and beneficial properties of kefir-isolated Enterococcus strains were investigated for the first time. Enterococcus strains were identified as Enterococcus durans using phenotypical and molecular characterisation methods. Neither resistance to clinically important antibiotics, nor genes encoding for eight different virulence factors were found. Interestingly, selected E. durans strains were able to inhibit different Gram+ and Gram− pathogens. These strains were able to survive simulated gastrointestinal conditions and showed a similar power of adhesion to mucins. Of note, the E. durans strains possessed anti-inflammatory properties as demonstrated by a significant inhibition of flagellin-induced response on Caco-2 cells. Our results indicate that the presence of E. durans in kefir does not represent a threat to consumers' health, and shows their potential both functionality and as probiotics.
Fil: Carasi, Paula. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Jacquot, Claudine. Universite de Bordeaux; Francia
Fil: Romanin, David Emmanuel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Elie, Anne Marie. Universite de Bordeaux; Francia
Fil: de Antoni, Graciela Liliana. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
Fil: Urdaci, María C.. Universite de Bordeaux; Francia
Fil: Serradell, María de los Ángeles. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Enterococcus
Probiotic
Safety
Kefir
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/31411
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/31411
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Safety and potential beneficial properties of Enterococcus strains isolated from kefirCarasi, PaulaJacquot, ClaudineRomanin, David EmmanuelElie, Anne Mariede Antoni, Graciela LilianaUrdaci, María C.Serradell, María de los ÁngelesEnterococcusProbioticSafetyKefirhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Kefir is a fermented milk containing microorganisms showing several properties beneficial to health. In the present work the safety aspects and beneficial properties of kefir-isolated Enterococcus strains were investigated for the first time. Enterococcus strains were identified as Enterococcus durans using phenotypical and molecular characterisation methods. Neither resistance to clinically important antibiotics, nor genes encoding for eight different virulence factors were found. Interestingly, selected E. durans strains were able to inhibit different Gram+ and Gram− pathogens. These strains were able to survive simulated gastrointestinal conditions and showed a similar power of adhesion to mucins. Of note, the E. durans strains possessed anti-inflammatory properties as demonstrated by a significant inhibition of flagellin-induced response on Caco-2 cells. Our results indicate that the presence of E. durans in kefir does not represent a threat to consumers' health, and shows their potential both functionality and as probiotics.Fil: Carasi, Paula. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Jacquot, Claudine. Universite de Bordeaux; FranciaFil: Romanin, David Emmanuel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Elie, Anne Marie. Universite de Bordeaux; FranciaFil: de Antoni, Graciela Liliana. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; ArgentinaFil: Urdaci, María C.. Universite de Bordeaux; FranciaFil: Serradell, María de los Ángeles. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2014-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31411Carasi, Paula; Jacquot, Claudine; Romanin, David Emmanuel; Elie, Anne Marie; de Antoni, Graciela Liliana; et al.; Safety and potential beneficial properties of Enterococcus strains isolated from kefir; Elsevier; International Dairy Journal; 39; 1; 7-2014; 193-2000958-6946CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0958694614001277info:eu-repo/semantics/altIdentifier/doi/10.1016/j.idairyj.2014.06.009info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:42:36Zoai:ri.conicet.gov.ar:11336/31411instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:42:36.748CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Safety and potential beneficial properties of Enterococcus strains isolated from kefir
title Safety and potential beneficial properties of Enterococcus strains isolated from kefir
spellingShingle Safety and potential beneficial properties of Enterococcus strains isolated from kefir
Carasi, Paula
Enterococcus
Probiotic
Safety
Kefir
title_short Safety and potential beneficial properties of Enterococcus strains isolated from kefir
title_full Safety and potential beneficial properties of Enterococcus strains isolated from kefir
title_fullStr Safety and potential beneficial properties of Enterococcus strains isolated from kefir
title_full_unstemmed Safety and potential beneficial properties of Enterococcus strains isolated from kefir
title_sort Safety and potential beneficial properties of Enterococcus strains isolated from kefir
dc.creator.none.fl_str_mv Carasi, Paula
Jacquot, Claudine
Romanin, David Emmanuel
Elie, Anne Marie
de Antoni, Graciela Liliana
Urdaci, María C.
Serradell, María de los Ángeles
author Carasi, Paula
author_facet Carasi, Paula
Jacquot, Claudine
Romanin, David Emmanuel
Elie, Anne Marie
de Antoni, Graciela Liliana
Urdaci, María C.
Serradell, María de los Ángeles
author_role author
author2 Jacquot, Claudine
Romanin, David Emmanuel
Elie, Anne Marie
de Antoni, Graciela Liliana
Urdaci, María C.
Serradell, María de los Ángeles
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Enterococcus
Probiotic
Safety
Kefir
topic Enterococcus
Probiotic
Safety
Kefir
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Kefir is a fermented milk containing microorganisms showing several properties beneficial to health. In the present work the safety aspects and beneficial properties of kefir-isolated Enterococcus strains were investigated for the first time. Enterococcus strains were identified as Enterococcus durans using phenotypical and molecular characterisation methods. Neither resistance to clinically important antibiotics, nor genes encoding for eight different virulence factors were found. Interestingly, selected E. durans strains were able to inhibit different Gram+ and Gram− pathogens. These strains were able to survive simulated gastrointestinal conditions and showed a similar power of adhesion to mucins. Of note, the E. durans strains possessed anti-inflammatory properties as demonstrated by a significant inhibition of flagellin-induced response on Caco-2 cells. Our results indicate that the presence of E. durans in kefir does not represent a threat to consumers' health, and shows their potential both functionality and as probiotics.
Fil: Carasi, Paula. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Jacquot, Claudine. Universite de Bordeaux; Francia
Fil: Romanin, David Emmanuel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Elie, Anne Marie. Universite de Bordeaux; Francia
Fil: de Antoni, Graciela Liliana. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
Fil: Urdaci, María C.. Universite de Bordeaux; Francia
Fil: Serradell, María de los Ángeles. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Kefir is a fermented milk containing microorganisms showing several properties beneficial to health. In the present work the safety aspects and beneficial properties of kefir-isolated Enterococcus strains were investigated for the first time. Enterococcus strains were identified as Enterococcus durans using phenotypical and molecular characterisation methods. Neither resistance to clinically important antibiotics, nor genes encoding for eight different virulence factors were found. Interestingly, selected E. durans strains were able to inhibit different Gram+ and Gram− pathogens. These strains were able to survive simulated gastrointestinal conditions and showed a similar power of adhesion to mucins. Of note, the E. durans strains possessed anti-inflammatory properties as demonstrated by a significant inhibition of flagellin-induced response on Caco-2 cells. Our results indicate that the presence of E. durans in kefir does not represent a threat to consumers' health, and shows their potential both functionality and as probiotics.
publishDate 2014
dc.date.none.fl_str_mv 2014-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/31411
Carasi, Paula; Jacquot, Claudine; Romanin, David Emmanuel; Elie, Anne Marie; de Antoni, Graciela Liliana; et al.; Safety and potential beneficial properties of Enterococcus strains isolated from kefir; Elsevier; International Dairy Journal; 39; 1; 7-2014; 193-200
0958-6946
CONICET Digital
CONICET
url http://hdl.handle.net/11336/31411
identifier_str_mv Carasi, Paula; Jacquot, Claudine; Romanin, David Emmanuel; Elie, Anne Marie; de Antoni, Graciela Liliana; et al.; Safety and potential beneficial properties of Enterococcus strains isolated from kefir; Elsevier; International Dairy Journal; 39; 1; 7-2014; 193-200
0958-6946
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0958694614001277
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.idairyj.2014.06.009
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083534047936512
score 13.22299

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