Selective response to iron and epo signals of iron cycle proteins in a mouse model
- Autores
- Fernandez Delias, María Florencia; Roque, Marta Elena
- Año de publicación
- 2020
- Idioma
- español castellano
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- The Erythropoietin (EPO) is associated with iron mobilization.The aim was to analyze the regulatory relationship between iron and EPO studying iron key proteins in several tissues in an animal model of iron overload and EPO. CF1 mice divided into groups (n= 4/group): 1) Iron-adequate (IA); 2) Iron-overload (IO) (iron saccharate; days 0, 4, 8, and 12 i.p. ; 1,800 mg/kg) ; 3) EPO (days 17, 18, and 19) i.p. ; 20,000 UI/kg); 4) Iron overload+EPO (IO+EPO). Immunohistochemistry: anti-DMT1 (divalent metal transporter1) and ZIP14 (Zrt-Irt-like Protein14). Perl´s staining. Iron levels: Wiener kit. The Protocol was approved by CICUAE-UNS. Our data demostrated that the protective action of EPO against IO was selective in several tissues responding to different signals as follows. In lung: both DMT1 and ZIP 14 response to “EPO signal”. Interestingly was observed that DMT1 localization in bronchial cells was changed being cytoplasmic in IA/IO+EPO/EPO, while it was localized in membrane cell and apical zone in IO condition. ZIP14 expression was dowregulated by “EPO signal” in bronchial cells. In spleen: both importers were downregulated by “EPO signal”. Conversely, hepatic tissue responds to iron signal. Hepatic DMT1 and ZIP14 were dowregulated and upregulated, respectively. On contrary,in pancreas a selective importers response to “iron/EPO signal” was observed. In fact, DMT1 expression in Langerhans islets was downregulated by iron signal, however in acini ZIP14 was downregulated by “EPO signal”. In all tissues Iron level was significant higher in the IO respect to IA and a significant decrease in IO+EPO respect to IO. The protective action of “EPO signal” against IO in all studied tissues could be explain by the reduced iron uptake in spleen, lung and pancreas. Nevertheless, the prevalence of the “iron signal” in liver may be explained by the increased hepatic iron uptake through ZIP14, thus reducing iron systemic level. Understanding the relations between these proteins will contribute to extend our knowledge in the field of iron and erythropoiesis.
Fil: Fernandez Delias, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Roque, Marta Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Reunión Anual de Sociedades de Biociencia
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Nanomedicinas
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio - Materia
-
IRON
EPO
TISSUES
MOUSE MODEL - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/154558
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Selective response to iron and epo signals of iron cycle proteins in a mouse modelFernandez Delias, María FlorenciaRoque, Marta ElenaIRONEPOTISSUESMOUSE MODELhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The Erythropoietin (EPO) is associated with iron mobilization.The aim was to analyze the regulatory relationship between iron and EPO studying iron key proteins in several tissues in an animal model of iron overload and EPO. CF1 mice divided into groups (n= 4/group): 1) Iron-adequate (IA); 2) Iron-overload (IO) (iron saccharate; days 0, 4, 8, and 12 i.p. ; 1,800 mg/kg) ; 3) EPO (days 17, 18, and 19) i.p. ; 20,000 UI/kg); 4) Iron overload+EPO (IO+EPO). Immunohistochemistry: anti-DMT1 (divalent metal transporter1) and ZIP14 (Zrt-Irt-like Protein14). Perl´s staining. Iron levels: Wiener kit. The Protocol was approved by CICUAE-UNS. Our data demostrated that the protective action of EPO against IO was selective in several tissues responding to different signals as follows. In lung: both DMT1 and ZIP 14 response to “EPO signal”. Interestingly was observed that DMT1 localization in bronchial cells was changed being cytoplasmic in IA/IO+EPO/EPO, while it was localized in membrane cell and apical zone in IO condition. ZIP14 expression was dowregulated by “EPO signal” in bronchial cells. In spleen: both importers were downregulated by “EPO signal”. Conversely, hepatic tissue responds to iron signal. Hepatic DMT1 and ZIP14 were dowregulated and upregulated, respectively. On contrary,in pancreas a selective importers response to “iron/EPO signal” was observed. In fact, DMT1 expression in Langerhans islets was downregulated by iron signal, however in acini ZIP14 was downregulated by “EPO signal”. In all tissues Iron level was significant higher in the IO respect to IA and a significant decrease in IO+EPO respect to IO. The protective action of “EPO signal” against IO in all studied tissues could be explain by the reduced iron uptake in spleen, lung and pancreas. Nevertheless, the prevalence of the “iron signal” in liver may be explained by the increased hepatic iron uptake through ZIP14, thus reducing iron systemic level. Understanding the relations between these proteins will contribute to extend our knowledge in the field of iron and erythropoiesis.Fil: Fernandez Delias, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Roque, Marta Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaReunión Anual de Sociedades de BiocienciaMar del PlataArgentinaSociedad Argentina de Investigación ClínicaAsociación Argentina de Farmacología ExperimentalSociedad Argentina de BiologíaSociedad Argentina de ProtozoologíaAsociación Argentina de NanomedicinasAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioFundación Revista Medicina2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/154558Selective response to iron and epo signals of iron cycle proteins in a mouse model; Reunión Anual de Sociedades de Biociencia; Mar del Plata; Argentina; 2019; 1-80025-76801669-9106CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol79-19/s4/vol79_s4.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:37:55Zoai:ri.conicet.gov.ar:11336/154558instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:37:56.082CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Selective response to iron and epo signals of iron cycle proteins in a mouse model |
title |
Selective response to iron and epo signals of iron cycle proteins in a mouse model |
spellingShingle |
Selective response to iron and epo signals of iron cycle proteins in a mouse model Fernandez Delias, María Florencia IRON EPO TISSUES MOUSE MODEL |
title_short |
Selective response to iron and epo signals of iron cycle proteins in a mouse model |
title_full |
Selective response to iron and epo signals of iron cycle proteins in a mouse model |
title_fullStr |
Selective response to iron and epo signals of iron cycle proteins in a mouse model |
title_full_unstemmed |
Selective response to iron and epo signals of iron cycle proteins in a mouse model |
title_sort |
Selective response to iron and epo signals of iron cycle proteins in a mouse model |
dc.creator.none.fl_str_mv |
Fernandez Delias, María Florencia Roque, Marta Elena |
author |
Fernandez Delias, María Florencia |
author_facet |
Fernandez Delias, María Florencia Roque, Marta Elena |
author_role |
author |
author2 |
Roque, Marta Elena |
author2_role |
author |
dc.subject.none.fl_str_mv |
IRON EPO TISSUES MOUSE MODEL |
topic |
IRON EPO TISSUES MOUSE MODEL |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
The Erythropoietin (EPO) is associated with iron mobilization.The aim was to analyze the regulatory relationship between iron and EPO studying iron key proteins in several tissues in an animal model of iron overload and EPO. CF1 mice divided into groups (n= 4/group): 1) Iron-adequate (IA); 2) Iron-overload (IO) (iron saccharate; days 0, 4, 8, and 12 i.p. ; 1,800 mg/kg) ; 3) EPO (days 17, 18, and 19) i.p. ; 20,000 UI/kg); 4) Iron overload+EPO (IO+EPO). Immunohistochemistry: anti-DMT1 (divalent metal transporter1) and ZIP14 (Zrt-Irt-like Protein14). Perl´s staining. Iron levels: Wiener kit. The Protocol was approved by CICUAE-UNS. Our data demostrated that the protective action of EPO against IO was selective in several tissues responding to different signals as follows. In lung: both DMT1 and ZIP 14 response to “EPO signal”. Interestingly was observed that DMT1 localization in bronchial cells was changed being cytoplasmic in IA/IO+EPO/EPO, while it was localized in membrane cell and apical zone in IO condition. ZIP14 expression was dowregulated by “EPO signal” in bronchial cells. In spleen: both importers were downregulated by “EPO signal”. Conversely, hepatic tissue responds to iron signal. Hepatic DMT1 and ZIP14 were dowregulated and upregulated, respectively. On contrary,in pancreas a selective importers response to “iron/EPO signal” was observed. In fact, DMT1 expression in Langerhans islets was downregulated by iron signal, however in acini ZIP14 was downregulated by “EPO signal”. In all tissues Iron level was significant higher in the IO respect to IA and a significant decrease in IO+EPO respect to IO. The protective action of “EPO signal” against IO in all studied tissues could be explain by the reduced iron uptake in spleen, lung and pancreas. Nevertheless, the prevalence of the “iron signal” in liver may be explained by the increased hepatic iron uptake through ZIP14, thus reducing iron systemic level. Understanding the relations between these proteins will contribute to extend our knowledge in the field of iron and erythropoiesis. Fil: Fernandez Delias, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina Fil: Roque, Marta Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina Reunión Anual de Sociedades de Biociencia Mar del Plata Argentina Sociedad Argentina de Investigación Clínica Asociación Argentina de Farmacología Experimental Sociedad Argentina de Biología Sociedad Argentina de Protozoología Asociación Argentina de Nanomedicinas Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio |
description |
The Erythropoietin (EPO) is associated with iron mobilization.The aim was to analyze the regulatory relationship between iron and EPO studying iron key proteins in several tissues in an animal model of iron overload and EPO. CF1 mice divided into groups (n= 4/group): 1) Iron-adequate (IA); 2) Iron-overload (IO) (iron saccharate; days 0, 4, 8, and 12 i.p. ; 1,800 mg/kg) ; 3) EPO (days 17, 18, and 19) i.p. ; 20,000 UI/kg); 4) Iron overload+EPO (IO+EPO). Immunohistochemistry: anti-DMT1 (divalent metal transporter1) and ZIP14 (Zrt-Irt-like Protein14). Perl´s staining. Iron levels: Wiener kit. The Protocol was approved by CICUAE-UNS. Our data demostrated that the protective action of EPO against IO was selective in several tissues responding to different signals as follows. In lung: both DMT1 and ZIP 14 response to “EPO signal”. Interestingly was observed that DMT1 localization in bronchial cells was changed being cytoplasmic in IA/IO+EPO/EPO, while it was localized in membrane cell and apical zone in IO condition. ZIP14 expression was dowregulated by “EPO signal” in bronchial cells. In spleen: both importers were downregulated by “EPO signal”. Conversely, hepatic tissue responds to iron signal. Hepatic DMT1 and ZIP14 were dowregulated and upregulated, respectively. On contrary,in pancreas a selective importers response to “iron/EPO signal” was observed. In fact, DMT1 expression in Langerhans islets was downregulated by iron signal, however in acini ZIP14 was downregulated by “EPO signal”. In all tissues Iron level was significant higher in the IO respect to IA and a significant decrease in IO+EPO respect to IO. The protective action of “EPO signal” against IO in all studied tissues could be explain by the reduced iron uptake in spleen, lung and pancreas. Nevertheless, the prevalence of the “iron signal” in liver may be explained by the increased hepatic iron uptake through ZIP14, thus reducing iron systemic level. Understanding the relations between these proteins will contribute to extend our knowledge in the field of iron and erythropoiesis. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Reunión Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/154558 Selective response to iron and epo signals of iron cycle proteins in a mouse model; Reunión Anual de Sociedades de Biociencia; Mar del Plata; Argentina; 2019; 1-8 0025-7680 1669-9106 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/154558 |
identifier_str_mv |
Selective response to iron and epo signals of iron cycle proteins in a mouse model; Reunión Anual de Sociedades de Biociencia; Mar del Plata; Argentina; 2019; 1-8 0025-7680 1669-9106 CONICET Digital CONICET |
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language |
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info:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol79-19/s4/vol79_s4.pdf |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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Fundación Revista Medicina |
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Fundación Revista Medicina |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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