Selective response to iron and epo signals of iron cycle proteins in a mouse model

Autores
Fernandez Delias, María Florencia; Roque, Marta Elena
Año de publicación
2020
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The Erythropoietin (EPO) is associated with iron mobilization.The aim was to analyze the regulatory relationship between iron and EPO studying iron key proteins in several tissues in an animal model of iron overload and EPO. CF1 mice divided into groups (n= 4/group): 1) Iron-adequate (IA); 2) Iron-overload (IO) (iron saccharate; days 0, 4, 8, and 12 i.p. ; 1,800 mg/kg) ; 3) EPO (days 17, 18, and 19) i.p. ; 20,000 UI/kg); 4) Iron overload+EPO (IO+EPO). Immunohistochemistry: anti-DMT1 (divalent metal transporter1) and ZIP14 (Zrt-Irt-like Protein14). Perl´s staining. Iron levels: Wiener kit. The Protocol was approved by CICUAE-UNS. Our data demostrated that the protective action of EPO against IO was selective in several tissues responding to different signals as follows. In lung: both DMT1 and ZIP 14 response to “EPO signal”. Interestingly was observed that DMT1 localization in bronchial cells was changed being cytoplasmic in IA/IO+EPO/EPO, while it was localized in membrane cell and apical zone in IO condition. ZIP14 expression was dowregulated by “EPO signal” in bronchial cells. In spleen: both importers were downregulated by “EPO signal”. Conversely, hepatic tissue responds to iron signal. Hepatic DMT1 and ZIP14 were dowregulated and upregulated, respectively. On contrary,in pancreas a selective importers response to “iron/EPO signal” was observed. In fact, DMT1 expression in Langerhans islets was downregulated by iron signal, however in acini ZIP14 was downregulated by “EPO signal”. In all tissues Iron level was significant higher in the IO respect to IA and a significant decrease in IO+EPO respect to IO. The protective action of “EPO signal” against IO in all studied tissues could be explain by the reduced iron uptake in spleen, lung and pancreas. Nevertheless, the prevalence of the “iron signal” in liver may be explained by the increased hepatic iron uptake through ZIP14, thus reducing iron systemic level. Understanding the relations between these proteins will contribute to extend our knowledge in the field of iron and erythropoiesis.
Fil: Fernandez Delias, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Roque, Marta Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Reunión Anual de Sociedades de Biociencia
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Nanomedicinas
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Materia
IRON
EPO
TISSUES
MOUSE MODEL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/154558
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/154558
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Selective response to iron and epo signals of iron cycle proteins in a mouse modelFernandez Delias, María FlorenciaRoque, Marta ElenaIRONEPOTISSUESMOUSE MODELhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The Erythropoietin (EPO) is associated with iron mobilization.The aim was to analyze the regulatory relationship between iron and EPO studying iron key proteins in several tissues in an animal model of iron overload and EPO. CF1 mice divided into groups (n= 4/group): 1) Iron-adequate (IA); 2) Iron-overload (IO) (iron saccharate; days 0, 4, 8, and 12 i.p. ; 1,800 mg/kg) ; 3) EPO (days 17, 18, and 19) i.p. ; 20,000 UI/kg); 4) Iron overload+EPO (IO+EPO). Immunohistochemistry: anti-DMT1 (divalent metal transporter1) and ZIP14 (Zrt-Irt-like Protein14). Perl´s staining. Iron levels: Wiener kit. The Protocol was approved by CICUAE-UNS. Our data demostrated that the protective action of EPO against IO was selective in several tissues responding to different signals as follows. In lung: both DMT1 and ZIP 14 response to “EPO signal”. Interestingly was observed that DMT1 localization in bronchial cells was changed being cytoplasmic in IA/IO+EPO/EPO, while it was localized in membrane cell and apical zone in IO condition. ZIP14 expression was dowregulated by “EPO signal” in bronchial cells. In spleen: both importers were downregulated by “EPO signal”. Conversely, hepatic tissue responds to iron signal. Hepatic DMT1 and ZIP14 were dowregulated and upregulated, respectively. On contrary,in pancreas a selective importers response to “iron/EPO signal” was observed. In fact, DMT1 expression in Langerhans islets was downregulated by iron signal, however in acini ZIP14 was downregulated by “EPO signal”. In all tissues Iron level was significant higher in the IO respect to IA and a significant decrease in IO+EPO respect to IO. The protective action of “EPO signal” against IO in all studied tissues could be explain by the reduced iron uptake in spleen, lung and pancreas. Nevertheless, the prevalence of the “iron signal” in liver may be explained by the increased hepatic iron uptake through ZIP14, thus reducing iron systemic level. Understanding the relations between these proteins will contribute to extend our knowledge in the field of iron and erythropoiesis.Fil: Fernandez Delias, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Roque, Marta Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaReunión Anual de Sociedades de BiocienciaMar del PlataArgentinaSociedad Argentina de Investigación ClínicaAsociación Argentina de Farmacología ExperimentalSociedad Argentina de BiologíaSociedad Argentina de ProtozoologíaAsociación Argentina de NanomedicinasAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioFundación Revista Medicina2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/154558Selective response to iron and epo signals of iron cycle proteins in a mouse model; Reunión Anual de Sociedades de Biociencia; Mar del Plata; Argentina; 2019; 1-80025-76801669-9106CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol79-19/s4/vol79_s4.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:06:06Zoai:ri.conicet.gov.ar:11336/154558instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:06:07.129CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Selective response to iron and epo signals of iron cycle proteins in a mouse model
title Selective response to iron and epo signals of iron cycle proteins in a mouse model
spellingShingle Selective response to iron and epo signals of iron cycle proteins in a mouse model
Fernandez Delias, María Florencia
IRON
EPO
TISSUES
MOUSE MODEL
title_short Selective response to iron and epo signals of iron cycle proteins in a mouse model
title_full Selective response to iron and epo signals of iron cycle proteins in a mouse model
title_fullStr Selective response to iron and epo signals of iron cycle proteins in a mouse model
title_full_unstemmed Selective response to iron and epo signals of iron cycle proteins in a mouse model
title_sort Selective response to iron and epo signals of iron cycle proteins in a mouse model
dc.creator.none.fl_str_mv Fernandez Delias, María Florencia
Roque, Marta Elena
author Fernandez Delias, María Florencia
author_facet Fernandez Delias, María Florencia
Roque, Marta Elena
author_role author
author2 Roque, Marta Elena
author2_role author
dc.subject.none.fl_str_mv IRON
EPO
TISSUES
MOUSE MODEL
topic IRON
EPO
TISSUES
MOUSE MODEL
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The Erythropoietin (EPO) is associated with iron mobilization.The aim was to analyze the regulatory relationship between iron and EPO studying iron key proteins in several tissues in an animal model of iron overload and EPO. CF1 mice divided into groups (n= 4/group): 1) Iron-adequate (IA); 2) Iron-overload (IO) (iron saccharate; days 0, 4, 8, and 12 i.p. ; 1,800 mg/kg) ; 3) EPO (days 17, 18, and 19) i.p. ; 20,000 UI/kg); 4) Iron overload+EPO (IO+EPO). Immunohistochemistry: anti-DMT1 (divalent metal transporter1) and ZIP14 (Zrt-Irt-like Protein14). Perl´s staining. Iron levels: Wiener kit. The Protocol was approved by CICUAE-UNS. Our data demostrated that the protective action of EPO against IO was selective in several tissues responding to different signals as follows. In lung: both DMT1 and ZIP 14 response to “EPO signal”. Interestingly was observed that DMT1 localization in bronchial cells was changed being cytoplasmic in IA/IO+EPO/EPO, while it was localized in membrane cell and apical zone in IO condition. ZIP14 expression was dowregulated by “EPO signal” in bronchial cells. In spleen: both importers were downregulated by “EPO signal”. Conversely, hepatic tissue responds to iron signal. Hepatic DMT1 and ZIP14 were dowregulated and upregulated, respectively. On contrary,in pancreas a selective importers response to “iron/EPO signal” was observed. In fact, DMT1 expression in Langerhans islets was downregulated by iron signal, however in acini ZIP14 was downregulated by “EPO signal”. In all tissues Iron level was significant higher in the IO respect to IA and a significant decrease in IO+EPO respect to IO. The protective action of “EPO signal” against IO in all studied tissues could be explain by the reduced iron uptake in spleen, lung and pancreas. Nevertheless, the prevalence of the “iron signal” in liver may be explained by the increased hepatic iron uptake through ZIP14, thus reducing iron systemic level. Understanding the relations between these proteins will contribute to extend our knowledge in the field of iron and erythropoiesis.
Fil: Fernandez Delias, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Roque, Marta Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Reunión Anual de Sociedades de Biociencia
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Sociedad Argentina de Biología
Sociedad Argentina de Protozoología
Asociación Argentina de Nanomedicinas
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
description The Erythropoietin (EPO) is associated with iron mobilization.The aim was to analyze the regulatory relationship between iron and EPO studying iron key proteins in several tissues in an animal model of iron overload and EPO. CF1 mice divided into groups (n= 4/group): 1) Iron-adequate (IA); 2) Iron-overload (IO) (iron saccharate; days 0, 4, 8, and 12 i.p. ; 1,800 mg/kg) ; 3) EPO (days 17, 18, and 19) i.p. ; 20,000 UI/kg); 4) Iron overload+EPO (IO+EPO). Immunohistochemistry: anti-DMT1 (divalent metal transporter1) and ZIP14 (Zrt-Irt-like Protein14). Perl´s staining. Iron levels: Wiener kit. The Protocol was approved by CICUAE-UNS. Our data demostrated that the protective action of EPO against IO was selective in several tissues responding to different signals as follows. In lung: both DMT1 and ZIP 14 response to “EPO signal”. Interestingly was observed that DMT1 localization in bronchial cells was changed being cytoplasmic in IA/IO+EPO/EPO, while it was localized in membrane cell and apical zone in IO condition. ZIP14 expression was dowregulated by “EPO signal” in bronchial cells. In spleen: both importers were downregulated by “EPO signal”. Conversely, hepatic tissue responds to iron signal. Hepatic DMT1 and ZIP14 were dowregulated and upregulated, respectively. On contrary,in pancreas a selective importers response to “iron/EPO signal” was observed. In fact, DMT1 expression in Langerhans islets was downregulated by iron signal, however in acini ZIP14 was downregulated by “EPO signal”. In all tissues Iron level was significant higher in the IO respect to IA and a significant decrease in IO+EPO respect to IO. The protective action of “EPO signal” against IO in all studied tissues could be explain by the reduced iron uptake in spleen, lung and pancreas. Nevertheless, the prevalence of the “iron signal” in liver may be explained by the increased hepatic iron uptake through ZIP14, thus reducing iron systemic level. Understanding the relations between these proteins will contribute to extend our knowledge in the field of iron and erythropoiesis.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/154558
Selective response to iron and epo signals of iron cycle proteins in a mouse model; Reunión Anual de Sociedades de Biociencia; Mar del Plata; Argentina; 2019; 1-8
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/154558
identifier_str_mv Selective response to iron and epo signals of iron cycle proteins in a mouse model; Reunión Anual de Sociedades de Biociencia; Mar del Plata; Argentina; 2019; 1-8
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol79-19/s4/vol79_s4.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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