Amelioration of lipopolysaccharide-induced acute kidney injury by erythropoietin : involvement of mitochondria-regulated apoptosis

Autores
Stoyanoff, Tania Romina; Todaro, Juan Santiago; Aguirre, María Victoria; Zimmermann, María Carla; Brandan, Nora Cristina
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Stoyanoff, Tania Romina. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina.
Fil: Stoyanoff, Tania Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Secretaria Ciencia y Técnica. Centro Científico Tecnológico Nordeste; Argentina.
Fil: Todaro, Juan Santiago. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina.
Fil: Todaro, Juan Santiago. Universidad Nacional del Nordeste. Secretaria General de Ciencia y Técnica; Argentina.
Fil: Aguirre, María Victoria. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina.
Fil: Zimmermann, María Carla. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina.
Fil: Zimmermann, María Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste; Argentina.
Fil: Brandan, Nora Cristina. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina.
Fil: Brandan, Nora Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste; Argentina.
Sepsis remains the most important cause of acute kidney injury (AKI) in critically ill patients and is an independent predictor of poor outcome. The administration of lipopolysaccharide (LPS) to animals reproduces most of the clinical features of sepsis, including AKI, a condition associated with renal cellular dysfunction and apoptosis. Erythropoietin (EPO) is a well known cytoprotective multifunctional hormone, which exerts antiinflammatory, anti-oxidant, anti-apoptotic and angiogenic effects in several tissues. The aim of this study was to evaluate the underlying mechanisms of EPO renoprotection through the expression of the EPO receptor (EPO-R) and the modulation of the intrinsic apoptotic pathway in LPS-induced AKI. Male inbred Balb/c mice were divided in four experimental groups: Control, LPS (8 mg/kg i.p.), EPO (3000 IU sc) and LPS + EPO. Assessment of renal function, histological examination, TUNEL in situ assay, immunohistochemistry and Western blottings of caspase-3, Bax, Bcl-xL, EPO-R and Cytochrome c were performed at 24 h post treatment. LPS + EPO treatment significantly improved renal function and ameliorated histopathological injury when compared to the LPS treated group. Results showed that EPO treatment attenuates renal tubular apoptosis through: (a) the overexpression of EPO-R in tubular interstitial cells, (b) the reduction of Bax/Bcl-xL ratio, (c) the inhibition Cytochrome c release into the cytosol and (d) the decrease of the active caspase-3 expression. This study suggests that EPO exerts renoprotection on an experimental model of LPS-induced AKI. EPO induced renoprotection involves an anti-apoptotic effect through the expression of EPO-R and the regulation of the mitochondrial apoptotic pathway.
Fuente
Toxicology, 2014, vol. 318, p. 13-21.
Materia
Sepsis
EPO induced renoprotection
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
Institución
Universidad Nacional del Nordeste
OAI Identificador
oai:repositorio.unne.edu.ar:123456789/33813

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repository_id_str 4871
network_name_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling Amelioration of lipopolysaccharide-induced acute kidney injury by erythropoietin : involvement of mitochondria-regulated apoptosisStoyanoff, Tania RominaTodaro, Juan SantiagoAguirre, María VictoriaZimmermann, María CarlaBrandan, Nora CristinaSepsisEPO induced renoprotectionFil: Stoyanoff, Tania Romina. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina.Fil: Stoyanoff, Tania Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Secretaria Ciencia y Técnica. Centro Científico Tecnológico Nordeste; Argentina.Fil: Todaro, Juan Santiago. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina.Fil: Todaro, Juan Santiago. Universidad Nacional del Nordeste. Secretaria General de Ciencia y Técnica; Argentina.Fil: Aguirre, María Victoria. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina.Fil: Zimmermann, María Carla. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina.Fil: Zimmermann, María Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste; Argentina.Fil: Brandan, Nora Cristina. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina.Fil: Brandan, Nora Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste; Argentina.Sepsis remains the most important cause of acute kidney injury (AKI) in critically ill patients and is an independent predictor of poor outcome. The administration of lipopolysaccharide (LPS) to animals reproduces most of the clinical features of sepsis, including AKI, a condition associated with renal cellular dysfunction and apoptosis. Erythropoietin (EPO) is a well known cytoprotective multifunctional hormone, which exerts antiinflammatory, anti-oxidant, anti-apoptotic and angiogenic effects in several tissues. The aim of this study was to evaluate the underlying mechanisms of EPO renoprotection through the expression of the EPO receptor (EPO-R) and the modulation of the intrinsic apoptotic pathway in LPS-induced AKI. Male inbred Balb/c mice were divided in four experimental groups: Control, LPS (8 mg/kg i.p.), EPO (3000 IU sc) and LPS + EPO. Assessment of renal function, histological examination, TUNEL in situ assay, immunohistochemistry and Western blottings of caspase-3, Bax, Bcl-xL, EPO-R and Cytochrome c were performed at 24 h post treatment. LPS + EPO treatment significantly improved renal function and ameliorated histopathological injury when compared to the LPS treated group. Results showed that EPO treatment attenuates renal tubular apoptosis through: (a) the overexpression of EPO-R in tubular interstitial cells, (b) the reduction of Bax/Bcl-xL ratio, (c) the inhibition Cytochrome c release into the cytosol and (d) the decrease of the active caspase-3 expression. This study suggests that EPO exerts renoprotection on an experimental model of LPS-induced AKI. EPO induced renoprotection involves an anti-apoptotic effect through the expression of EPO-R and the regulation of the mitochondrial apoptotic pathway.Elsevier Ireland Ltd2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfStoyanoff, Tania Romina, et al, 2014. Amelioration of lipopolysaccharide-induced acute kidney injury by erythropoietin : Involvement of mitochondria-regulated. Toxicology. Ámsterdam: Elsevier Ireland Ltd, vol. 318, p. 13-21. ISSN 0300-483X.http://repositorio.unne.edu.ar/handle/123456789/33813Toxicology, 2014, vol. 318, p. 13-21.reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordesteenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentina2025-09-29T14:29:10Zoai:repositorio.unne.edu.ar:123456789/33813instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712025-09-29 14:29:10.835Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv Amelioration of lipopolysaccharide-induced acute kidney injury by erythropoietin : involvement of mitochondria-regulated apoptosis
title Amelioration of lipopolysaccharide-induced acute kidney injury by erythropoietin : involvement of mitochondria-regulated apoptosis
spellingShingle Amelioration of lipopolysaccharide-induced acute kidney injury by erythropoietin : involvement of mitochondria-regulated apoptosis
Stoyanoff, Tania Romina
Sepsis
EPO induced renoprotection
title_short Amelioration of lipopolysaccharide-induced acute kidney injury by erythropoietin : involvement of mitochondria-regulated apoptosis
title_full Amelioration of lipopolysaccharide-induced acute kidney injury by erythropoietin : involvement of mitochondria-regulated apoptosis
title_fullStr Amelioration of lipopolysaccharide-induced acute kidney injury by erythropoietin : involvement of mitochondria-regulated apoptosis
title_full_unstemmed Amelioration of lipopolysaccharide-induced acute kidney injury by erythropoietin : involvement of mitochondria-regulated apoptosis
title_sort Amelioration of lipopolysaccharide-induced acute kidney injury by erythropoietin : involvement of mitochondria-regulated apoptosis
dc.creator.none.fl_str_mv Stoyanoff, Tania Romina
Todaro, Juan Santiago
Aguirre, María Victoria
Zimmermann, María Carla
Brandan, Nora Cristina
author Stoyanoff, Tania Romina
author_facet Stoyanoff, Tania Romina
Todaro, Juan Santiago
Aguirre, María Victoria
Zimmermann, María Carla
Brandan, Nora Cristina
author_role author
author2 Todaro, Juan Santiago
Aguirre, María Victoria
Zimmermann, María Carla
Brandan, Nora Cristina
author2_role author
author
author
author
dc.subject.none.fl_str_mv Sepsis
EPO induced renoprotection
topic Sepsis
EPO induced renoprotection
dc.description.none.fl_txt_mv Fil: Stoyanoff, Tania Romina. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina.
Fil: Stoyanoff, Tania Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Secretaria Ciencia y Técnica. Centro Científico Tecnológico Nordeste; Argentina.
Fil: Todaro, Juan Santiago. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina.
Fil: Todaro, Juan Santiago. Universidad Nacional del Nordeste. Secretaria General de Ciencia y Técnica; Argentina.
Fil: Aguirre, María Victoria. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina.
Fil: Zimmermann, María Carla. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina.
Fil: Zimmermann, María Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste; Argentina.
Fil: Brandan, Nora Cristina. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina.
Fil: Brandan, Nora Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste; Argentina.
Sepsis remains the most important cause of acute kidney injury (AKI) in critically ill patients and is an independent predictor of poor outcome. The administration of lipopolysaccharide (LPS) to animals reproduces most of the clinical features of sepsis, including AKI, a condition associated with renal cellular dysfunction and apoptosis. Erythropoietin (EPO) is a well known cytoprotective multifunctional hormone, which exerts antiinflammatory, anti-oxidant, anti-apoptotic and angiogenic effects in several tissues. The aim of this study was to evaluate the underlying mechanisms of EPO renoprotection through the expression of the EPO receptor (EPO-R) and the modulation of the intrinsic apoptotic pathway in LPS-induced AKI. Male inbred Balb/c mice were divided in four experimental groups: Control, LPS (8 mg/kg i.p.), EPO (3000 IU sc) and LPS + EPO. Assessment of renal function, histological examination, TUNEL in situ assay, immunohistochemistry and Western blottings of caspase-3, Bax, Bcl-xL, EPO-R and Cytochrome c were performed at 24 h post treatment. LPS + EPO treatment significantly improved renal function and ameliorated histopathological injury when compared to the LPS treated group. Results showed that EPO treatment attenuates renal tubular apoptosis through: (a) the overexpression of EPO-R in tubular interstitial cells, (b) the reduction of Bax/Bcl-xL ratio, (c) the inhibition Cytochrome c release into the cytosol and (d) the decrease of the active caspase-3 expression. This study suggests that EPO exerts renoprotection on an experimental model of LPS-induced AKI. EPO induced renoprotection involves an anti-apoptotic effect through the expression of EPO-R and the regulation of the mitochondrial apoptotic pathway.
description Fil: Stoyanoff, Tania Romina. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Stoyanoff, Tania Romina, et al, 2014. Amelioration of lipopolysaccharide-induced acute kidney injury by erythropoietin : Involvement of mitochondria-regulated. Toxicology. Ámsterdam: Elsevier Ireland Ltd, vol. 318, p. 13-21. ISSN 0300-483X.
http://repositorio.unne.edu.ar/handle/123456789/33813
identifier_str_mv Stoyanoff, Tania Romina, et al, 2014. Amelioration of lipopolysaccharide-induced acute kidney injury by erythropoietin : Involvement of mitochondria-regulated. Toxicology. Ámsterdam: Elsevier Ireland Ltd, vol. 318, p. 13-21. ISSN 0300-483X.
url http://repositorio.unne.edu.ar/handle/123456789/33813
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland Ltd
publisher.none.fl_str_mv Elsevier Ireland Ltd
dc.source.none.fl_str_mv Toxicology, 2014, vol. 318, p. 13-21.
reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname:Universidad Nacional del Nordeste
reponame_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str Universidad Nacional del Nordeste
repository.name.fl_str_mv Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
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