Performance of four transport and storage systems for molecular detection of multidrug-resistant tuberculosis

Autores
Rabodoarivelo, Marie Sylvianne; Imperiale, Belén Rocío; Andrianiavomikotroka, Rina; Brandao, Angela; Kumar, Parveen; Singh, Sarman; Ferrazoli, Lucilaine; Morcillo, Nora; Rasolofo, Voahangy; Palomino, Juan Carlos; Vandamme, Peter; Martin, Anandi
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background Detection of drug-resistant tuberculosis is essential for the control of the disease but it is often hampered by the limitation of transport and storage of samples from remote locations to the reference laboratory. We performed a retrospective field study to evaluate the performance of four supports enabling the transport and storage of samples to be used for molecular detection of drug resistance using the GenoType MTBDRplus. Methods Two hundred Mycobacterium tuberculosis strains were selected and spotted on slides, FTA cards, GenoCards, and in ethanol. GenoType MTBDRplus was subsequently performed with the DNA extracted from these supports. Sensitivity and specificity were calculated and compared to the results obtained by drug susceptibility testing. Results For all supports, the overall sensitivity and specificity for detection of resistance to RIF was between 95% and 100%, and for INH between 95% and 98%. Conclusion The four transport and storage supports showed a good sensitivity and specificity for the detection of resistance to RIF and INH in M. tuberculosis strains using the GenoType MTBDRplus. These supports can be maintained at room temperature and could represent an important alternative cost-effective method useful for rapid molecular detection of drugresistant TB in low-resource settings.
Fil: Rabodoarivelo, Marie Sylvianne. University of Ghent; Bélgica
Fil: Imperiale, Belén Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: Andrianiavomikotroka, Rina. Institut Pasteur de Madagascar; Madagascar
Fil: Brandao, Angela. Fundación Oswaldo Cruz; Brasil
Fil: Kumar, Parveen. All India Institute of Medical Sciences; India
Fil: Singh, Sarman. All India Institute of Medical Sciences; India
Fil: Ferrazoli, Lucilaine. Instituto Adolfo Lutz Núcleo de Tuberculose e Micobacteriosis Sao Paulo; Brasil
Fil: Morcillo, Nora. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: Rasolofo, Voahangy. Institut Pasteur de Madagascar; Madagascar
Fil: Palomino, Juan Carlos. University of Ghent; Bélgica
Fil: Vandamme, Peter. University of Ghent; Bélgica
Fil: Martin, Anandi. University of Ghent; Bélgica
Materia
GenoType MTBDRplus.
DRUG RESISTANT M. TUBERCULOSIS
TRANSPORT AND STORAGE SYSTEM
MOLECULAR DRUG RESISTANCE DETECTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/85779
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/85779
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Performance of four transport and storage systems for molecular detection of multidrug-resistant tuberculosisRabodoarivelo, Marie SylvianneImperiale, Belén RocíoAndrianiavomikotroka, RinaBrandao, AngelaKumar, ParveenSingh, SarmanFerrazoli, LucilaineMorcillo, NoraRasolofo, VoahangyPalomino, Juan CarlosVandamme, PeterMartin, AnandiGenoType MTBDRplus.DRUG RESISTANT M. TUBERCULOSISTRANSPORT AND STORAGE SYSTEMMOLECULAR DRUG RESISTANCE DETECTIONhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Background Detection of drug-resistant tuberculosis is essential for the control of the disease but it is often hampered by the limitation of transport and storage of samples from remote locations to the reference laboratory. We performed a retrospective field study to evaluate the performance of four supports enabling the transport and storage of samples to be used for molecular detection of drug resistance using the GenoType MTBDRplus. Methods Two hundred Mycobacterium tuberculosis strains were selected and spotted on slides, FTA cards, GenoCards, and in ethanol. GenoType MTBDRplus was subsequently performed with the DNA extracted from these supports. Sensitivity and specificity were calculated and compared to the results obtained by drug susceptibility testing. Results For all supports, the overall sensitivity and specificity for detection of resistance to RIF was between 95% and 100%, and for INH between 95% and 98%. Conclusion The four transport and storage supports showed a good sensitivity and specificity for the detection of resistance to RIF and INH in M. tuberculosis strains using the GenoType MTBDRplus. These supports can be maintained at room temperature and could represent an important alternative cost-effective method useful for rapid molecular detection of drugresistant TB in low-resource settings.Fil: Rabodoarivelo, Marie Sylvianne. University of Ghent; BélgicaFil: Imperiale, Belén Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; ArgentinaFil: Andrianiavomikotroka, Rina. Institut Pasteur de Madagascar; MadagascarFil: Brandao, Angela. Fundación Oswaldo Cruz; BrasilFil: Kumar, Parveen. All India Institute of Medical Sciences; IndiaFil: Singh, Sarman. All India Institute of Medical Sciences; IndiaFil: Ferrazoli, Lucilaine. Instituto Adolfo Lutz Núcleo de Tuberculose e Micobacteriosis Sao Paulo; BrasilFil: Morcillo, Nora. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; ArgentinaFil: Rasolofo, Voahangy. Institut Pasteur de Madagascar; MadagascarFil: Palomino, Juan Carlos. University of Ghent; BélgicaFil: Vandamme, Peter. University of Ghent; BélgicaFil: Martin, Anandi. University of Ghent; BélgicaPublic Library of Science2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/85779Rabodoarivelo, Marie Sylvianne; Imperiale, Belén Rocío; Andrianiavomikotroka, Rina; Brandao, Angela; Kumar, Parveen; et al.; Performance of four transport and storage systems for molecular detection of multidrug-resistant tuberculosis; Public Library of Science; Plos One; 10; 10; 10-2015; 1-111932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0139382info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0139382info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:22Zoai:ri.conicet.gov.ar:11336/85779instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:23.142CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Performance of four transport and storage systems for molecular detection of multidrug-resistant tuberculosis
title Performance of four transport and storage systems for molecular detection of multidrug-resistant tuberculosis
spellingShingle Performance of four transport and storage systems for molecular detection of multidrug-resistant tuberculosis
Rabodoarivelo, Marie Sylvianne
GenoType MTBDRplus.
DRUG RESISTANT M. TUBERCULOSIS
TRANSPORT AND STORAGE SYSTEM
MOLECULAR DRUG RESISTANCE DETECTION
title_short Performance of four transport and storage systems for molecular detection of multidrug-resistant tuberculosis
title_full Performance of four transport and storage systems for molecular detection of multidrug-resistant tuberculosis
title_fullStr Performance of four transport and storage systems for molecular detection of multidrug-resistant tuberculosis
title_full_unstemmed Performance of four transport and storage systems for molecular detection of multidrug-resistant tuberculosis
title_sort Performance of four transport and storage systems for molecular detection of multidrug-resistant tuberculosis
dc.creator.none.fl_str_mv Rabodoarivelo, Marie Sylvianne
Imperiale, Belén Rocío
Andrianiavomikotroka, Rina
Brandao, Angela
Kumar, Parveen
Singh, Sarman
Ferrazoli, Lucilaine
Morcillo, Nora
Rasolofo, Voahangy
Palomino, Juan Carlos
Vandamme, Peter
Martin, Anandi
author Rabodoarivelo, Marie Sylvianne
author_facet Rabodoarivelo, Marie Sylvianne
Imperiale, Belén Rocío
Andrianiavomikotroka, Rina
Brandao, Angela
Kumar, Parveen
Singh, Sarman
Ferrazoli, Lucilaine
Morcillo, Nora
Rasolofo, Voahangy
Palomino, Juan Carlos
Vandamme, Peter
Martin, Anandi
author_role author
author2 Imperiale, Belén Rocío
Andrianiavomikotroka, Rina
Brandao, Angela
Kumar, Parveen
Singh, Sarman
Ferrazoli, Lucilaine
Morcillo, Nora
Rasolofo, Voahangy
Palomino, Juan Carlos
Vandamme, Peter
Martin, Anandi
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv GenoType MTBDRplus.
DRUG RESISTANT M. TUBERCULOSIS
TRANSPORT AND STORAGE SYSTEM
MOLECULAR DRUG RESISTANCE DETECTION
topic GenoType MTBDRplus.
DRUG RESISTANT M. TUBERCULOSIS
TRANSPORT AND STORAGE SYSTEM
MOLECULAR DRUG RESISTANCE DETECTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background Detection of drug-resistant tuberculosis is essential for the control of the disease but it is often hampered by the limitation of transport and storage of samples from remote locations to the reference laboratory. We performed a retrospective field study to evaluate the performance of four supports enabling the transport and storage of samples to be used for molecular detection of drug resistance using the GenoType MTBDRplus. Methods Two hundred Mycobacterium tuberculosis strains were selected and spotted on slides, FTA cards, GenoCards, and in ethanol. GenoType MTBDRplus was subsequently performed with the DNA extracted from these supports. Sensitivity and specificity were calculated and compared to the results obtained by drug susceptibility testing. Results For all supports, the overall sensitivity and specificity for detection of resistance to RIF was between 95% and 100%, and for INH between 95% and 98%. Conclusion The four transport and storage supports showed a good sensitivity and specificity for the detection of resistance to RIF and INH in M. tuberculosis strains using the GenoType MTBDRplus. These supports can be maintained at room temperature and could represent an important alternative cost-effective method useful for rapid molecular detection of drugresistant TB in low-resource settings.
Fil: Rabodoarivelo, Marie Sylvianne. University of Ghent; Bélgica
Fil: Imperiale, Belén Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: Andrianiavomikotroka, Rina. Institut Pasteur de Madagascar; Madagascar
Fil: Brandao, Angela. Fundación Oswaldo Cruz; Brasil
Fil: Kumar, Parveen. All India Institute of Medical Sciences; India
Fil: Singh, Sarman. All India Institute of Medical Sciences; India
Fil: Ferrazoli, Lucilaine. Instituto Adolfo Lutz Núcleo de Tuberculose e Micobacteriosis Sao Paulo; Brasil
Fil: Morcillo, Nora. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: Rasolofo, Voahangy. Institut Pasteur de Madagascar; Madagascar
Fil: Palomino, Juan Carlos. University of Ghent; Bélgica
Fil: Vandamme, Peter. University of Ghent; Bélgica
Fil: Martin, Anandi. University of Ghent; Bélgica
description Background Detection of drug-resistant tuberculosis is essential for the control of the disease but it is often hampered by the limitation of transport and storage of samples from remote locations to the reference laboratory. We performed a retrospective field study to evaluate the performance of four supports enabling the transport and storage of samples to be used for molecular detection of drug resistance using the GenoType MTBDRplus. Methods Two hundred Mycobacterium tuberculosis strains were selected and spotted on slides, FTA cards, GenoCards, and in ethanol. GenoType MTBDRplus was subsequently performed with the DNA extracted from these supports. Sensitivity and specificity were calculated and compared to the results obtained by drug susceptibility testing. Results For all supports, the overall sensitivity and specificity for detection of resistance to RIF was between 95% and 100%, and for INH between 95% and 98%. Conclusion The four transport and storage supports showed a good sensitivity and specificity for the detection of resistance to RIF and INH in M. tuberculosis strains using the GenoType MTBDRplus. These supports can be maintained at room temperature and could represent an important alternative cost-effective method useful for rapid molecular detection of drugresistant TB in low-resource settings.
publishDate 2015
dc.date.none.fl_str_mv 2015-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/85779
Rabodoarivelo, Marie Sylvianne; Imperiale, Belén Rocío; Andrianiavomikotroka, Rina; Brandao, Angela; Kumar, Parveen; et al.; Performance of four transport and storage systems for molecular detection of multidrug-resistant tuberculosis; Public Library of Science; Plos One; 10; 10; 10-2015; 1-11
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/85779
identifier_str_mv Rabodoarivelo, Marie Sylvianne; Imperiale, Belén Rocío; Andrianiavomikotroka, Rina; Brandao, Angela; Kumar, Parveen; et al.; Performance of four transport and storage systems for molecular detection of multidrug-resistant tuberculosis; Public Library of Science; Plos One; 10; 10; 10-2015; 1-11
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0139382
info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0139382
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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