Detection of multidrug-resistant tuberculosis from stored DNA Samples: A multicenter study

Autores
Rabodoarivelo, Marie Sylvianne; Brandao, A.; Novella, M.C. Cergole; Bombonatte, A.G.C.; Imperiale, Belén Rocío; Rakotosamimanana, N.; Morcillo, Nora Susana; Rasolofo, V.; Palomino, Juan Carlos; Martin, A.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: In low-income countries, rapid detection of tuberculosis (TB) drug resistance is often restricted by the difficulties of transporting and storing sputum samples from remote health centers to the reference laboratories where molecular tests are available. The aim of this study was to evaluate the performance of four transport and storage systems for molecular detection of rifampicin (RIF) and isoniazid (INH) resistance. Methods: This was a multicenter study. Molecular detection of RIF and INH resistance was performed directly from smear-positive TB sputa spotted on a slide, FTA card, GenoCard, and ethanol using the Genotype MTBDRplus assay. The performance of the DNA extraction method from each storage support to detect drug resistance was assessed by calculating their sensitivity and specificity compared to the phenotypic method. Results: From all sites, the overall sensitivity and specificity for RIF-resistance detection was 88% and 85%, respectively, for slides, 86% and 92%, respectively, for GenoCard, 87% and 89%, respectively, for FTA card, and 88% and 92%, respectively, for ethanol. For INH-resistance detection, the overall sensitivity and specificity was 82% and 90%, respectively, for slides, 85% and 96%, respectively, for GenoCard, 86% and 92%, respectively, for FTA card, and 86% and 94%, respectively, for ethanol. Conclusion: Smear slides and filter cards showed to be very useful tools to facilitate DNA extraction from sputum samples with the potential to accelerate the detection of drug resistance in remote areas.
Fil: Rabodoarivelo, Marie Sylvianne. Instituto Pasteur; Madagascar. Université d'Antananarivo; Madagascar
Fil: Brandao, A.. Instituto Adolfo Lutz; Brasil. Fundación Oswaldo Cruz; Brasil
Fil: Novella, M.C. Cergole. Instituto Adolfo Lutz; Brasil
Fil: Bombonatte, A.G.C.. Instituto Adolfo Lutz; Brasil
Fil: Imperiale, Belén Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Rakotosamimanana, N.. Instituto Pasteur; Madagascar
Fil: Morcillo, Nora Susana. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: Rasolofo, V.. Instituto Pasteur; Madagascar. Université d'Antananarivo; Madagascar
Fil: Palomino, Juan Carlos. University of Ghent; Bélgica
Fil: Martin, A.. Université Catholique de Louvain; Bélgica
Materia
FILTER CARDS
MOLECULAR DIAGNOSIS
MULTIDRUG-RESISTANT DETECTION
MYCOBACTERIUM TUBERCULOSIS
SMEAR SLIDES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/95991
Acceder
id CONICETDig_0bf32bf50f554f8abe9cd81f5fe603a7
oai_identifier_str oai:ri.conicet.gov.ar:11336/95991
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Detection of multidrug-resistant tuberculosis from stored DNA Samples: A multicenter studyRabodoarivelo, Marie SylvianneBrandao, A.Novella, M.C. CergoleBombonatte, A.G.C.Imperiale, Belén RocíoRakotosamimanana, N.Morcillo, Nora SusanaRasolofo, V.Palomino, Juan CarlosMartin, A.FILTER CARDSMOLECULAR DIAGNOSISMULTIDRUG-RESISTANT DETECTIONMYCOBACTERIUM TUBERCULOSISSMEAR SLIDEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: In low-income countries, rapid detection of tuberculosis (TB) drug resistance is often restricted by the difficulties of transporting and storing sputum samples from remote health centers to the reference laboratories where molecular tests are available. The aim of this study was to evaluate the performance of four transport and storage systems for molecular detection of rifampicin (RIF) and isoniazid (INH) resistance. Methods: This was a multicenter study. Molecular detection of RIF and INH resistance was performed directly from smear-positive TB sputa spotted on a slide, FTA card, GenoCard, and ethanol using the Genotype MTBDRplus assay. The performance of the DNA extraction method from each storage support to detect drug resistance was assessed by calculating their sensitivity and specificity compared to the phenotypic method. Results: From all sites, the overall sensitivity and specificity for RIF-resistance detection was 88% and 85%, respectively, for slides, 86% and 92%, respectively, for GenoCard, 87% and 89%, respectively, for FTA card, and 88% and 92%, respectively, for ethanol. For INH-resistance detection, the overall sensitivity and specificity was 82% and 90%, respectively, for slides, 85% and 96%, respectively, for GenoCard, 86% and 92%, respectively, for FTA card, and 86% and 94%, respectively, for ethanol. Conclusion: Smear slides and filter cards showed to be very useful tools to facilitate DNA extraction from sputum samples with the potential to accelerate the detection of drug resistance in remote areas.Fil: Rabodoarivelo, Marie Sylvianne. Instituto Pasteur; Madagascar. Université d'Antananarivo; MadagascarFil: Brandao, A.. Instituto Adolfo Lutz; Brasil. Fundación Oswaldo Cruz; BrasilFil: Novella, M.C. Cergole. Instituto Adolfo Lutz; BrasilFil: Bombonatte, A.G.C.. Instituto Adolfo Lutz; BrasilFil: Imperiale, Belén Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Rakotosamimanana, N.. Instituto Pasteur; MadagascarFil: Morcillo, Nora Susana. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; ArgentinaFil: Rasolofo, V.. Instituto Pasteur; Madagascar. Université d'Antananarivo; MadagascarFil: Palomino, Juan Carlos. University of Ghent; BélgicaFil: Martin, A.. Université Catholique de Louvain; BélgicaMedknow Publications2018-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/95991Rabodoarivelo, Marie Sylvianne; Brandao, A.; Novella, M.C. Cergole; Bombonatte, A.G.C.; Imperiale, Belén Rocío; et al.; Detection of multidrug-resistant tuberculosis from stored DNA Samples: A multicenter study; Medknow Publications; International Journal of Mycobacteriology; 7; 1; 1-2018; 40-442212-55312212-554XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4103/ijmy.ijmy_193_17info:eu-repo/semantics/altIdentifier/url/ijmyco.org/article.asp?issn=2212-5531;year=2018;volume=7;issue=1;spage=40;epage=44;aulast=Rabodoariveloinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:14:52Zoai:ri.conicet.gov.ar:11336/95991instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:14:52.413CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Detection of multidrug-resistant tuberculosis from stored DNA Samples: A multicenter study
title Detection of multidrug-resistant tuberculosis from stored DNA Samples: A multicenter study
spellingShingle Detection of multidrug-resistant tuberculosis from stored DNA Samples: A multicenter study
Rabodoarivelo, Marie Sylvianne
FILTER CARDS
MOLECULAR DIAGNOSIS
MULTIDRUG-RESISTANT DETECTION
MYCOBACTERIUM TUBERCULOSIS
SMEAR SLIDES
title_short Detection of multidrug-resistant tuberculosis from stored DNA Samples: A multicenter study
title_full Detection of multidrug-resistant tuberculosis from stored DNA Samples: A multicenter study
title_fullStr Detection of multidrug-resistant tuberculosis from stored DNA Samples: A multicenter study
title_full_unstemmed Detection of multidrug-resistant tuberculosis from stored DNA Samples: A multicenter study
title_sort Detection of multidrug-resistant tuberculosis from stored DNA Samples: A multicenter study
dc.creator.none.fl_str_mv Rabodoarivelo, Marie Sylvianne
Brandao, A.
Novella, M.C. Cergole
Bombonatte, A.G.C.
Imperiale, Belén Rocío
Rakotosamimanana, N.
Morcillo, Nora Susana
Rasolofo, V.
Palomino, Juan Carlos
Martin, A.
author Rabodoarivelo, Marie Sylvianne
author_facet Rabodoarivelo, Marie Sylvianne
Brandao, A.
Novella, M.C. Cergole
Bombonatte, A.G.C.
Imperiale, Belén Rocío
Rakotosamimanana, N.
Morcillo, Nora Susana
Rasolofo, V.
Palomino, Juan Carlos
Martin, A.
author_role author
author2 Brandao, A.
Novella, M.C. Cergole
Bombonatte, A.G.C.
Imperiale, Belén Rocío
Rakotosamimanana, N.
Morcillo, Nora Susana
Rasolofo, V.
Palomino, Juan Carlos
Martin, A.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv FILTER CARDS
MOLECULAR DIAGNOSIS
MULTIDRUG-RESISTANT DETECTION
MYCOBACTERIUM TUBERCULOSIS
SMEAR SLIDES
topic FILTER CARDS
MOLECULAR DIAGNOSIS
MULTIDRUG-RESISTANT DETECTION
MYCOBACTERIUM TUBERCULOSIS
SMEAR SLIDES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: In low-income countries, rapid detection of tuberculosis (TB) drug resistance is often restricted by the difficulties of transporting and storing sputum samples from remote health centers to the reference laboratories where molecular tests are available. The aim of this study was to evaluate the performance of four transport and storage systems for molecular detection of rifampicin (RIF) and isoniazid (INH) resistance. Methods: This was a multicenter study. Molecular detection of RIF and INH resistance was performed directly from smear-positive TB sputa spotted on a slide, FTA card, GenoCard, and ethanol using the Genotype MTBDRplus assay. The performance of the DNA extraction method from each storage support to detect drug resistance was assessed by calculating their sensitivity and specificity compared to the phenotypic method. Results: From all sites, the overall sensitivity and specificity for RIF-resistance detection was 88% and 85%, respectively, for slides, 86% and 92%, respectively, for GenoCard, 87% and 89%, respectively, for FTA card, and 88% and 92%, respectively, for ethanol. For INH-resistance detection, the overall sensitivity and specificity was 82% and 90%, respectively, for slides, 85% and 96%, respectively, for GenoCard, 86% and 92%, respectively, for FTA card, and 86% and 94%, respectively, for ethanol. Conclusion: Smear slides and filter cards showed to be very useful tools to facilitate DNA extraction from sputum samples with the potential to accelerate the detection of drug resistance in remote areas.
Fil: Rabodoarivelo, Marie Sylvianne. Instituto Pasteur; Madagascar. Université d'Antananarivo; Madagascar
Fil: Brandao, A.. Instituto Adolfo Lutz; Brasil. Fundación Oswaldo Cruz; Brasil
Fil: Novella, M.C. Cergole. Instituto Adolfo Lutz; Brasil
Fil: Bombonatte, A.G.C.. Instituto Adolfo Lutz; Brasil
Fil: Imperiale, Belén Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Rakotosamimanana, N.. Instituto Pasteur; Madagascar
Fil: Morcillo, Nora Susana. Provincia de Buenos Aires. Ministerio de Salud. Hospital "Dr. Antonio A. Cetrángolo"; Argentina
Fil: Rasolofo, V.. Instituto Pasteur; Madagascar. Université d'Antananarivo; Madagascar
Fil: Palomino, Juan Carlos. University of Ghent; Bélgica
Fil: Martin, A.. Université Catholique de Louvain; Bélgica
description Background: In low-income countries, rapid detection of tuberculosis (TB) drug resistance is often restricted by the difficulties of transporting and storing sputum samples from remote health centers to the reference laboratories where molecular tests are available. The aim of this study was to evaluate the performance of four transport and storage systems for molecular detection of rifampicin (RIF) and isoniazid (INH) resistance. Methods: This was a multicenter study. Molecular detection of RIF and INH resistance was performed directly from smear-positive TB sputa spotted on a slide, FTA card, GenoCard, and ethanol using the Genotype MTBDRplus assay. The performance of the DNA extraction method from each storage support to detect drug resistance was assessed by calculating their sensitivity and specificity compared to the phenotypic method. Results: From all sites, the overall sensitivity and specificity for RIF-resistance detection was 88% and 85%, respectively, for slides, 86% and 92%, respectively, for GenoCard, 87% and 89%, respectively, for FTA card, and 88% and 92%, respectively, for ethanol. For INH-resistance detection, the overall sensitivity and specificity was 82% and 90%, respectively, for slides, 85% and 96%, respectively, for GenoCard, 86% and 92%, respectively, for FTA card, and 86% and 94%, respectively, for ethanol. Conclusion: Smear slides and filter cards showed to be very useful tools to facilitate DNA extraction from sputum samples with the potential to accelerate the detection of drug resistance in remote areas.
publishDate 2018
dc.date.none.fl_str_mv 2018-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/95991
Rabodoarivelo, Marie Sylvianne; Brandao, A.; Novella, M.C. Cergole; Bombonatte, A.G.C.; Imperiale, Belén Rocío; et al.; Detection of multidrug-resistant tuberculosis from stored DNA Samples: A multicenter study; Medknow Publications; International Journal of Mycobacteriology; 7; 1; 1-2018; 40-44
2212-5531
2212-554X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/95991
identifier_str_mv Rabodoarivelo, Marie Sylvianne; Brandao, A.; Novella, M.C. Cergole; Bombonatte, A.G.C.; Imperiale, Belén Rocío; et al.; Detection of multidrug-resistant tuberculosis from stored DNA Samples: A multicenter study; Medknow Publications; International Journal of Mycobacteriology; 7; 1; 1-2018; 40-44
2212-5531
2212-554X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4103/ijmy.ijmy_193_17
info:eu-repo/semantics/altIdentifier/url/ijmyco.org/article.asp?issn=2212-5531;year=2018;volume=7;issue=1;spage=40;epage=44;aulast=Rabodoarivelo
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Medknow Publications
publisher.none.fl_str_mv Medknow Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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