Antibacterial Effect of Chitosan–Gold Nanoparticles and Computational Modeling of the Interaction between Chitosan and a Lipid Bilayer Model

Autores
Fuster, M.G.; Montalbán, M. G.; Carissimi, G.; Lima, Beatriz Viviana; Feresin, Gabriela Egly; Cano, M.; Giner Casares, J. J.; López Cascales, J.J.; Enriz, Ricardo Daniel; Víllora, G.
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pathogenic bacteria have the ability to develop antibiotic resistance mechanisms. Their action consists mainly in the production of bacterial enzymes that inactivate antibiotics or the appearance of modifications that prevent the arrival of the drug at the target point or the alteration of the target point itself, becoming a growing problem for health systems. Chitosan–gold nanoparticles (Cs-AuNPs) have been shown as effective bactericidal materials avoiding damage to human cells. In this work, Cs-AuNPs were synthesized using chitosan as the reducing agent, and a systematic analysis of the influence of the synthesis parameters on the size and zeta potential of the Cs-AuNPs and their UV-vis spectra was carried out. We used a simulation model to characterize the interaction of chitosan with bacterial membranes, using a symmetric charged bilayer and two different chitosan models with different degrees of the chitosan amine protonation as a function of pH, with the aim to elucidate the antibacterial mechanism involving the cell wall disruption. The Cs-AuNP antibacterial activity was evaluated to check the simulation model.
Fil: Fuster, M.G.. Universidad de Murcia. Facultad de Química; España
Fil: Montalbán, M. G.. Universidad de Murcia. Facultad de Química; España
Fil: Carissimi, G.. Universidad de Murcia. Facultad de Química; España
Fil: Lima, Beatriz Viviana. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: Feresin, Gabriela Egly. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: Cano, M.. Universidad de Cordoba. Instituto Universitario de Investigación En Química Fina y Nanoquímica.; España
Fil: Giner Casares, J. J.. Universidad de Cordoba. Instituto Universitario de Investigación En Química Fina y Nanoquímica.; España
Fil: López Cascales, J.J.. Universidad Politécnica de Cartagena; España
Fil: Enriz, Ricardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Víllora, G.. Universidad de Murcia. Facultad de Química; España
Materia
ANTIMICROBIAL ACTIVITY
CHITOSAN–GOLD NANOPARTICLE
COMPUTATIONAL MODEL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/146839
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/146839
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antibacterial Effect of Chitosan–Gold Nanoparticles and Computational Modeling of the Interaction between Chitosan and a Lipid Bilayer ModelFuster, M.G.Montalbán, M. G.Carissimi, G.Lima, Beatriz VivianaFeresin, Gabriela EglyCano, M.Giner Casares, J. J.López Cascales, J.J.Enriz, Ricardo DanielVíllora, G.ANTIMICROBIAL ACTIVITYCHITOSAN–GOLD NANOPARTICLECOMPUTATIONAL MODELhttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Pathogenic bacteria have the ability to develop antibiotic resistance mechanisms. Their action consists mainly in the production of bacterial enzymes that inactivate antibiotics or the appearance of modifications that prevent the arrival of the drug at the target point or the alteration of the target point itself, becoming a growing problem for health systems. Chitosan–gold nanoparticles (Cs-AuNPs) have been shown as effective bactericidal materials avoiding damage to human cells. In this work, Cs-AuNPs were synthesized using chitosan as the reducing agent, and a systematic analysis of the influence of the synthesis parameters on the size and zeta potential of the Cs-AuNPs and their UV-vis spectra was carried out. We used a simulation model to characterize the interaction of chitosan with bacterial membranes, using a symmetric charged bilayer and two different chitosan models with different degrees of the chitosan amine protonation as a function of pH, with the aim to elucidate the antibacterial mechanism involving the cell wall disruption. The Cs-AuNP antibacterial activity was evaluated to check the simulation model.Fil: Fuster, M.G.. Universidad de Murcia. Facultad de Química; EspañaFil: Montalbán, M. G.. Universidad de Murcia. Facultad de Química; EspañaFil: Carissimi, G.. Universidad de Murcia. Facultad de Química; EspañaFil: Lima, Beatriz Viviana. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; ArgentinaFil: Feresin, Gabriela Egly. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; ArgentinaFil: Cano, M.. Universidad de Cordoba. Instituto Universitario de Investigación En Química Fina y Nanoquímica.; EspañaFil: Giner Casares, J. J.. Universidad de Cordoba. Instituto Universitario de Investigación En Química Fina y Nanoquímica.; EspañaFil: López Cascales, J.J.. Universidad Politécnica de Cartagena; EspañaFil: Enriz, Ricardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Víllora, G.. Universidad de Murcia. Facultad de Química; EspañaMultidisciplinary Digital Publishing Institute2020-11-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/146839Fuster, M.G.; Montalbán, M. G.; Carissimi, G.; Lima, Beatriz Viviana; Feresin, Gabriela Egly; et al.; Antibacterial Effect of Chitosan–Gold Nanoparticles and Computational Modeling of the Interaction between Chitosan and a Lipid Bilayer Model; Multidisciplinary Digital Publishing Institute; Nanomaterials; 10; 12; 25-11-2020; 1-182079-4991CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2079-4991/10/12/2340info:eu-repo/semantics/altIdentifier/doi/10.3390/nano10122340info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:14Zoai:ri.conicet.gov.ar:11336/146839instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:15.0CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antibacterial Effect of Chitosan–Gold Nanoparticles and Computational Modeling of the Interaction between Chitosan and a Lipid Bilayer Model
title Antibacterial Effect of Chitosan–Gold Nanoparticles and Computational Modeling of the Interaction between Chitosan and a Lipid Bilayer Model
spellingShingle Antibacterial Effect of Chitosan–Gold Nanoparticles and Computational Modeling of the Interaction between Chitosan and a Lipid Bilayer Model
Fuster, M.G.
ANTIMICROBIAL ACTIVITY
CHITOSAN–GOLD NANOPARTICLE
COMPUTATIONAL MODEL
title_short Antibacterial Effect of Chitosan–Gold Nanoparticles and Computational Modeling of the Interaction between Chitosan and a Lipid Bilayer Model
title_full Antibacterial Effect of Chitosan–Gold Nanoparticles and Computational Modeling of the Interaction between Chitosan and a Lipid Bilayer Model
title_fullStr Antibacterial Effect of Chitosan–Gold Nanoparticles and Computational Modeling of the Interaction between Chitosan and a Lipid Bilayer Model
title_full_unstemmed Antibacterial Effect of Chitosan–Gold Nanoparticles and Computational Modeling of the Interaction between Chitosan and a Lipid Bilayer Model
title_sort Antibacterial Effect of Chitosan–Gold Nanoparticles and Computational Modeling of the Interaction between Chitosan and a Lipid Bilayer Model
dc.creator.none.fl_str_mv Fuster, M.G.
Montalbán, M. G.
Carissimi, G.
Lima, Beatriz Viviana
Feresin, Gabriela Egly
Cano, M.
Giner Casares, J. J.
López Cascales, J.J.
Enriz, Ricardo Daniel
Víllora, G.
author Fuster, M.G.
author_facet Fuster, M.G.
Montalbán, M. G.
Carissimi, G.
Lima, Beatriz Viviana
Feresin, Gabriela Egly
Cano, M.
Giner Casares, J. J.
López Cascales, J.J.
Enriz, Ricardo Daniel
Víllora, G.
author_role author
author2 Montalbán, M. G.
Carissimi, G.
Lima, Beatriz Viviana
Feresin, Gabriela Egly
Cano, M.
Giner Casares, J. J.
López Cascales, J.J.
Enriz, Ricardo Daniel
Víllora, G.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTIMICROBIAL ACTIVITY
CHITOSAN–GOLD NANOPARTICLE
COMPUTATIONAL MODEL
topic ANTIMICROBIAL ACTIVITY
CHITOSAN–GOLD NANOPARTICLE
COMPUTATIONAL MODEL
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Pathogenic bacteria have the ability to develop antibiotic resistance mechanisms. Their action consists mainly in the production of bacterial enzymes that inactivate antibiotics or the appearance of modifications that prevent the arrival of the drug at the target point or the alteration of the target point itself, becoming a growing problem for health systems. Chitosan–gold nanoparticles (Cs-AuNPs) have been shown as effective bactericidal materials avoiding damage to human cells. In this work, Cs-AuNPs were synthesized using chitosan as the reducing agent, and a systematic analysis of the influence of the synthesis parameters on the size and zeta potential of the Cs-AuNPs and their UV-vis spectra was carried out. We used a simulation model to characterize the interaction of chitosan with bacterial membranes, using a symmetric charged bilayer and two different chitosan models with different degrees of the chitosan amine protonation as a function of pH, with the aim to elucidate the antibacterial mechanism involving the cell wall disruption. The Cs-AuNP antibacterial activity was evaluated to check the simulation model.
Fil: Fuster, M.G.. Universidad de Murcia. Facultad de Química; España
Fil: Montalbán, M. G.. Universidad de Murcia. Facultad de Química; España
Fil: Carissimi, G.. Universidad de Murcia. Facultad de Química; España
Fil: Lima, Beatriz Viviana. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: Feresin, Gabriela Egly. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: Cano, M.. Universidad de Cordoba. Instituto Universitario de Investigación En Química Fina y Nanoquímica.; España
Fil: Giner Casares, J. J.. Universidad de Cordoba. Instituto Universitario de Investigación En Química Fina y Nanoquímica.; España
Fil: López Cascales, J.J.. Universidad Politécnica de Cartagena; España
Fil: Enriz, Ricardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Víllora, G.. Universidad de Murcia. Facultad de Química; España
description Pathogenic bacteria have the ability to develop antibiotic resistance mechanisms. Their action consists mainly in the production of bacterial enzymes that inactivate antibiotics or the appearance of modifications that prevent the arrival of the drug at the target point or the alteration of the target point itself, becoming a growing problem for health systems. Chitosan–gold nanoparticles (Cs-AuNPs) have been shown as effective bactericidal materials avoiding damage to human cells. In this work, Cs-AuNPs were synthesized using chitosan as the reducing agent, and a systematic analysis of the influence of the synthesis parameters on the size and zeta potential of the Cs-AuNPs and their UV-vis spectra was carried out. We used a simulation model to characterize the interaction of chitosan with bacterial membranes, using a symmetric charged bilayer and two different chitosan models with different degrees of the chitosan amine protonation as a function of pH, with the aim to elucidate the antibacterial mechanism involving the cell wall disruption. The Cs-AuNP antibacterial activity was evaluated to check the simulation model.
publishDate 2020
dc.date.none.fl_str_mv 2020-11-25
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/146839
Fuster, M.G.; Montalbán, M. G.; Carissimi, G.; Lima, Beatriz Viviana; Feresin, Gabriela Egly; et al.; Antibacterial Effect of Chitosan–Gold Nanoparticles and Computational Modeling of the Interaction between Chitosan and a Lipid Bilayer Model; Multidisciplinary Digital Publishing Institute; Nanomaterials; 10; 12; 25-11-2020; 1-18
2079-4991
CONICET Digital
CONICET
url http://hdl.handle.net/11336/146839
identifier_str_mv Fuster, M.G.; Montalbán, M. G.; Carissimi, G.; Lima, Beatriz Viviana; Feresin, Gabriela Egly; et al.; Antibacterial Effect of Chitosan–Gold Nanoparticles and Computational Modeling of the Interaction between Chitosan and a Lipid Bilayer Model; Multidisciplinary Digital Publishing Institute; Nanomaterials; 10; 12; 25-11-2020; 1-18
2079-4991
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2079-4991/10/12/2340
info:eu-repo/semantics/altIdentifier/doi/10.3390/nano10122340
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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