Neuroendocrine control of morphogenesis

Autores
Garelli, Andres; Volonté, Yanel Andrea; Viegas, Filipe; Pereirinha, Joana; Prado Spalm, Facundo Heber; Casimiro, Andreia; Macedo, Andre; Heredia, Fabiana; Gontijo, Alisson
Año de publicación
2017
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Drosophila larvae undergo a dramatic change in body shape at the end of the larval growth period: the long, transparent, and flexible larval cuticle is remodeled into the short, tanned, and rigid puparium, the skin inside which higher dipteran larvae undergo metamorphosis. This body change is achieved by a series of stereotyped muscular contractions accompanied by structural sclerotization of the cuticle. While the onset of the whole metamorphosis program is known to be under the control of the hormone ecdysone, the molecular pathways mediating the downstream behavioral and morphogenetic processes are less understood. Serendipitously, we observed that larvae lacking the conserved relaxin-receptor like G-protein coupled receptor (GPCR), Lgr3, fail to shorten into the characteristic puparium form, so that their cuticle sclerotizes into an elongated larva-like puparium instead. Tissue and cell-type-specific RNAi experiments revealed that this Lgr3 activity is separable from its previously described role during larval development, where it has been shown to act in a subpopulation of CNS neurons to coordinate imaginal disc growth with developmental timing, in a Drosophila insulin-like peptide 8 (Dilp8)-dependent manner. Rather, our results indicate that Lgr3 acts in a distinct population of CNS neurons that respond to a strong developmentally-triggered pulse of carcass-derived Dilp8 peptide, which occurs within a 30-60-min window at the onset of pupariation. Hence, the peptide hormone Dilp8 and the neuronal GPCR Lgr3 constitute a new interorgan communication pathway controlling puparium morphogenesis.
Fil: Garelli, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidade Nova de Lisboa; Portugal
Fil: Volonté, Yanel Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Viegas, Filipe. Universidade Nova de Lisboa; Portugal
Fil: Pereirinha, Joana. Universidade Nova de Lisboa; Portugal
Fil: Prado Spalm, Facundo Heber. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Casimiro, Andreia. Universidade Nova de Lisboa; Portugal
Fil: Macedo, Andre. Universidade Nova de Lisboa; Portugal
Fil: Heredia, Fabiana. Universidade Nova de Lisboa; Portugal
Fil: Gontijo, Alisson. Universidade Nova de Lisboa; Portugal
DrosTuga: 2017 Annual Portuguese Drosophila Meeting
Tomar
Portugal
Portuguese Drosophila
Materia
DROSOPHILA
METAMORPHOSIS
DILP8
LGR3
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/238169
Acceder
id CONICETDig_508464a7e53af9ea32c0b08d2cb211d8
oai_identifier_str oai:ri.conicet.gov.ar:11336/238169
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Neuroendocrine control of morphogenesisGarelli, AndresVolonté, Yanel AndreaViegas, FilipePereirinha, JoanaPrado Spalm, Facundo HeberCasimiro, AndreiaMacedo, AndreHeredia, FabianaGontijo, AlissonDROSOPHILAMETAMORPHOSISDILP8LGR3https://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3Drosophila larvae undergo a dramatic change in body shape at the end of the larval growth period: the long, transparent, and flexible larval cuticle is remodeled into the short, tanned, and rigid puparium, the skin inside which higher dipteran larvae undergo metamorphosis. This body change is achieved by a series of stereotyped muscular contractions accompanied by structural sclerotization of the cuticle. While the onset of the whole metamorphosis program is known to be under the control of the hormone ecdysone, the molecular pathways mediating the downstream behavioral and morphogenetic processes are less understood. Serendipitously, we observed that larvae lacking the conserved relaxin-receptor like G-protein coupled receptor (GPCR), Lgr3, fail to shorten into the characteristic puparium form, so that their cuticle sclerotizes into an elongated larva-like puparium instead. Tissue and cell-type-specific RNAi experiments revealed that this Lgr3 activity is separable from its previously described role during larval development, where it has been shown to act in a subpopulation of CNS neurons to coordinate imaginal disc growth with developmental timing, in a Drosophila insulin-like peptide 8 (Dilp8)-dependent manner. Rather, our results indicate that Lgr3 acts in a distinct population of CNS neurons that respond to a strong developmentally-triggered pulse of carcass-derived Dilp8 peptide, which occurs within a 30-60-min window at the onset of pupariation. Hence, the peptide hormone Dilp8 and the neuronal GPCR Lgr3 constitute a new interorgan communication pathway controlling puparium morphogenesis.Fil: Garelli, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidade Nova de Lisboa; PortugalFil: Volonté, Yanel Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Viegas, Filipe. Universidade Nova de Lisboa; PortugalFil: Pereirinha, Joana. Universidade Nova de Lisboa; PortugalFil: Prado Spalm, Facundo Heber. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Casimiro, Andreia. Universidade Nova de Lisboa; PortugalFil: Macedo, Andre. Universidade Nova de Lisboa; PortugalFil: Heredia, Fabiana. Universidade Nova de Lisboa; PortugalFil: Gontijo, Alisson. Universidade Nova de Lisboa; PortugalDrosTuga: 2017 Annual Portuguese Drosophila MeetingTomarPortugalPortuguese DrosophilaUniversidade Nova de LisboaMartinho, RuiMorais-de-Sá, EuricoMoreno, EduardoTelley, Ivo A.2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectEncuentroBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/238169Neuroendocrine control of morphogenesis; DrosTuga: 2017 Annual Portuguese Drosophila Meeting; Tomar; Portugal; 2017; 63-63CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://drosophilaresearch.org/event/2017-portugese-drosophila-meetingInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:28:12Zoai:ri.conicet.gov.ar:11336/238169instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:28:12.992CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Neuroendocrine control of morphogenesis
title Neuroendocrine control of morphogenesis
spellingShingle Neuroendocrine control of morphogenesis
Garelli, Andres
DROSOPHILA
METAMORPHOSIS
DILP8
LGR3
title_short Neuroendocrine control of morphogenesis
title_full Neuroendocrine control of morphogenesis
title_fullStr Neuroendocrine control of morphogenesis
title_full_unstemmed Neuroendocrine control of morphogenesis
title_sort Neuroendocrine control of morphogenesis
dc.creator.none.fl_str_mv Garelli, Andres
Volonté, Yanel Andrea
Viegas, Filipe
Pereirinha, Joana
Prado Spalm, Facundo Heber
Casimiro, Andreia
Macedo, Andre
Heredia, Fabiana
Gontijo, Alisson
author Garelli, Andres
author_facet Garelli, Andres
Volonté, Yanel Andrea
Viegas, Filipe
Pereirinha, Joana
Prado Spalm, Facundo Heber
Casimiro, Andreia
Macedo, Andre
Heredia, Fabiana
Gontijo, Alisson
author_role author
author2 Volonté, Yanel Andrea
Viegas, Filipe
Pereirinha, Joana
Prado Spalm, Facundo Heber
Casimiro, Andreia
Macedo, Andre
Heredia, Fabiana
Gontijo, Alisson
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Martinho, Rui
Morais-de-Sá, Eurico
Moreno, Eduardo
Telley, Ivo A.
dc.subject.none.fl_str_mv DROSOPHILA
METAMORPHOSIS
DILP8
LGR3
topic DROSOPHILA
METAMORPHOSIS
DILP8
LGR3
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Drosophila larvae undergo a dramatic change in body shape at the end of the larval growth period: the long, transparent, and flexible larval cuticle is remodeled into the short, tanned, and rigid puparium, the skin inside which higher dipteran larvae undergo metamorphosis. This body change is achieved by a series of stereotyped muscular contractions accompanied by structural sclerotization of the cuticle. While the onset of the whole metamorphosis program is known to be under the control of the hormone ecdysone, the molecular pathways mediating the downstream behavioral and morphogenetic processes are less understood. Serendipitously, we observed that larvae lacking the conserved relaxin-receptor like G-protein coupled receptor (GPCR), Lgr3, fail to shorten into the characteristic puparium form, so that their cuticle sclerotizes into an elongated larva-like puparium instead. Tissue and cell-type-specific RNAi experiments revealed that this Lgr3 activity is separable from its previously described role during larval development, where it has been shown to act in a subpopulation of CNS neurons to coordinate imaginal disc growth with developmental timing, in a Drosophila insulin-like peptide 8 (Dilp8)-dependent manner. Rather, our results indicate that Lgr3 acts in a distinct population of CNS neurons that respond to a strong developmentally-triggered pulse of carcass-derived Dilp8 peptide, which occurs within a 30-60-min window at the onset of pupariation. Hence, the peptide hormone Dilp8 and the neuronal GPCR Lgr3 constitute a new interorgan communication pathway controlling puparium morphogenesis.
Fil: Garelli, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidade Nova de Lisboa; Portugal
Fil: Volonté, Yanel Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Viegas, Filipe. Universidade Nova de Lisboa; Portugal
Fil: Pereirinha, Joana. Universidade Nova de Lisboa; Portugal
Fil: Prado Spalm, Facundo Heber. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Casimiro, Andreia. Universidade Nova de Lisboa; Portugal
Fil: Macedo, Andre. Universidade Nova de Lisboa; Portugal
Fil: Heredia, Fabiana. Universidade Nova de Lisboa; Portugal
Fil: Gontijo, Alisson. Universidade Nova de Lisboa; Portugal
DrosTuga: 2017 Annual Portuguese Drosophila Meeting
Tomar
Portugal
Portuguese Drosophila
description Drosophila larvae undergo a dramatic change in body shape at the end of the larval growth period: the long, transparent, and flexible larval cuticle is remodeled into the short, tanned, and rigid puparium, the skin inside which higher dipteran larvae undergo metamorphosis. This body change is achieved by a series of stereotyped muscular contractions accompanied by structural sclerotization of the cuticle. While the onset of the whole metamorphosis program is known to be under the control of the hormone ecdysone, the molecular pathways mediating the downstream behavioral and morphogenetic processes are less understood. Serendipitously, we observed that larvae lacking the conserved relaxin-receptor like G-protein coupled receptor (GPCR), Lgr3, fail to shorten into the characteristic puparium form, so that their cuticle sclerotizes into an elongated larva-like puparium instead. Tissue and cell-type-specific RNAi experiments revealed that this Lgr3 activity is separable from its previously described role during larval development, where it has been shown to act in a subpopulation of CNS neurons to coordinate imaginal disc growth with developmental timing, in a Drosophila insulin-like peptide 8 (Dilp8)-dependent manner. Rather, our results indicate that Lgr3 acts in a distinct population of CNS neurons that respond to a strong developmentally-triggered pulse of carcass-derived Dilp8 peptide, which occurs within a 30-60-min window at the onset of pupariation. Hence, the peptide hormone Dilp8 and the neuronal GPCR Lgr3 constitute a new interorgan communication pathway controlling puparium morphogenesis.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Encuentro
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/238169
Neuroendocrine control of morphogenesis; DrosTuga: 2017 Annual Portuguese Drosophila Meeting; Tomar; Portugal; 2017; 63-63
CONICET Digital
CONICET
url http://hdl.handle.net/11336/238169
identifier_str_mv Neuroendocrine control of morphogenesis; DrosTuga: 2017 Annual Portuguese Drosophila Meeting; Tomar; Portugal; 2017; 63-63
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://drosophilaresearch.org/event/2017-portugese-drosophila-meeting
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Universidade Nova de Lisboa
publisher.none.fl_str_mv Universidade Nova de Lisboa
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781857337704448
score 12.982451

Publicaciones similares