Ceramide-1-phosphate: a new mediator of survival and development in retina photoreceptors

Autores
Miranda, Gisela Edit; Abrahan, Carolina Elizabeth; Agnolazza, Daniela Luciana; Politi, Luis Enrique; Rotstein, Nora Patricia
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Purpose. Simple sphingolipids control crucial cellular processes in several cell types. We demonstrated that sphingolipids such as ceramide, sphingosine and sphingosine-1-phosphate are key mediators in the regulation of survival, differentiation and proliferation of retina photoreceptors. Ceramide-1-phosphate (C1P) regulates growth and survival in several cell types; however, little is known concerning its functions in the retina. We here explored whether C1P also participated in controlling photoreceptor development. Methods. Rat retina neuronal cultures were supplemented with 1-10 µM C1P. Proliferation was determined by evaluating 5-bromo-2-deoxyuridine (BrdU) uptake and number of mitotic figures and differentiation by establishing opsin and peripherin expression by immunocytochemistry and Western blot. Apoptosis was inhibited with the caspase pan-inhibitor ZVADFMK and evaluated by TUNEL assay, propidium iodide/annexin V and DAPI labeling. Preservation of mitochondrial membrane potential was evaluated. Results. C1P enhanced BrdU uptake and increased mitosis in retinal progenitors. C1P addition advanced photoreceptor differentiation, enhancing opsin and peripherin expression and stimulating development of apical processes, in which these proteins were concentrated. In the absence of their trophic factors, photoreceptors degenerate after 4 days in vitro and at day 6 almost 50% of photoreceptors were apoptotic; C1P decreased photoreceptor apoptosis, reducing this percentage by half. Inhibiting caspase activity reduced photoreceptor apoptosis in controls but did not increase opsin expression, implying C1P has separate effects on differentiation and survival. Conclusions. These results suggest for the first time that C1P is a novel mediator that plays multiple functions in photoreceptors, initially regulating their proliferation and then promoting their survival and differentiation.
Fil: Miranda, Gisela Edit. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Abrahan, Carolina Elizabeth. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Agnolazza, Daniela Luciana. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Politi, Luis Enrique. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Rotstein, Nora Patricia. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Materia
Fotorreceptor
Ceramida 1 Fosfato
Neuroprotección
Proliferación
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/6465
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/6465
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Ceramide-1-phosphate: a new mediator of survival and development in retina photoreceptorsMiranda, Gisela EditAbrahan, Carolina ElizabethAgnolazza, Daniela LucianaPoliti, Luis EnriqueRotstein, Nora PatriciaFotorreceptorCeramida 1 FosfatoNeuroprotecciónProliferaciónhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Purpose. Simple sphingolipids control crucial cellular processes in several cell types. We demonstrated that sphingolipids such as ceramide, sphingosine and sphingosine-1-phosphate are key mediators in the regulation of survival, differentiation and proliferation of retina photoreceptors. Ceramide-1-phosphate (C1P) regulates growth and survival in several cell types; however, little is known concerning its functions in the retina. We here explored whether C1P also participated in controlling photoreceptor development. Methods. Rat retina neuronal cultures were supplemented with 1-10 µM C1P. Proliferation was determined by evaluating 5-bromo-2-deoxyuridine (BrdU) uptake and number of mitotic figures and differentiation by establishing opsin and peripherin expression by immunocytochemistry and Western blot. Apoptosis was inhibited with the caspase pan-inhibitor ZVADFMK and evaluated by TUNEL assay, propidium iodide/annexin V and DAPI labeling. Preservation of mitochondrial membrane potential was evaluated. Results. C1P enhanced BrdU uptake and increased mitosis in retinal progenitors. C1P addition advanced photoreceptor differentiation, enhancing opsin and peripherin expression and stimulating development of apical processes, in which these proteins were concentrated. In the absence of their trophic factors, photoreceptors degenerate after 4 days in vitro and at day 6 almost 50% of photoreceptors were apoptotic; C1P decreased photoreceptor apoptosis, reducing this percentage by half. Inhibiting caspase activity reduced photoreceptor apoptosis in controls but did not increase opsin expression, implying C1P has separate effects on differentiation and survival. Conclusions. These results suggest for the first time that C1P is a novel mediator that plays multiple functions in photoreceptors, initially regulating their proliferation and then promoting their survival and differentiation.Fil: Miranda, Gisela Edit. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; ArgentinaFil: Abrahan, Carolina Elizabeth. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; ArgentinaFil: Agnolazza, Daniela Luciana. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; ArgentinaFil: Politi, Luis Enrique. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; ArgentinaFil: Rotstein, Nora Patricia. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; ArgentinaAssociation For Research In Vision And Ophthalmology2011-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6465Miranda, Gisela Edit; Abrahan, Carolina Elizabeth; Agnolazza, Daniela Luciana; Politi, Luis Enrique; Rotstein, Nora Patricia; Ceramide-1-phosphate: a new mediator of survival and development in retina photoreceptors; Association For Research In Vision And Ophthalmology; Investigative Ophthalmology & Visual Science; 52; 9; 8-2011; 6580-65880146-0404enginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/pmid/21724910info:eu-repo/semantics/altIdentifier/doi/10.1167/iovs.10-7065info:eu-repo/semantics/altIdentifier/url/http://iovs.arvojournals.org/article.aspx?articleid=2187942info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:04:47Zoai:ri.conicet.gov.ar:11336/6465instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:04:47.381CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Ceramide-1-phosphate: a new mediator of survival and development in retina photoreceptors
title Ceramide-1-phosphate: a new mediator of survival and development in retina photoreceptors
spellingShingle Ceramide-1-phosphate: a new mediator of survival and development in retina photoreceptors
Miranda, Gisela Edit
Fotorreceptor
Ceramida 1 Fosfato
Neuroprotección
Proliferación
title_short Ceramide-1-phosphate: a new mediator of survival and development in retina photoreceptors
title_full Ceramide-1-phosphate: a new mediator of survival and development in retina photoreceptors
title_fullStr Ceramide-1-phosphate: a new mediator of survival and development in retina photoreceptors
title_full_unstemmed Ceramide-1-phosphate: a new mediator of survival and development in retina photoreceptors
title_sort Ceramide-1-phosphate: a new mediator of survival and development in retina photoreceptors
dc.creator.none.fl_str_mv Miranda, Gisela Edit
Abrahan, Carolina Elizabeth
Agnolazza, Daniela Luciana
Politi, Luis Enrique
Rotstein, Nora Patricia
author Miranda, Gisela Edit
author_facet Miranda, Gisela Edit
Abrahan, Carolina Elizabeth
Agnolazza, Daniela Luciana
Politi, Luis Enrique
Rotstein, Nora Patricia
author_role author
author2 Abrahan, Carolina Elizabeth
Agnolazza, Daniela Luciana
Politi, Luis Enrique
Rotstein, Nora Patricia
author2_role author
author
author
author
dc.subject.none.fl_str_mv Fotorreceptor
Ceramida 1 Fosfato
Neuroprotección
Proliferación
topic Fotorreceptor
Ceramida 1 Fosfato
Neuroprotección
Proliferación
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Purpose. Simple sphingolipids control crucial cellular processes in several cell types. We demonstrated that sphingolipids such as ceramide, sphingosine and sphingosine-1-phosphate are key mediators in the regulation of survival, differentiation and proliferation of retina photoreceptors. Ceramide-1-phosphate (C1P) regulates growth and survival in several cell types; however, little is known concerning its functions in the retina. We here explored whether C1P also participated in controlling photoreceptor development. Methods. Rat retina neuronal cultures were supplemented with 1-10 µM C1P. Proliferation was determined by evaluating 5-bromo-2-deoxyuridine (BrdU) uptake and number of mitotic figures and differentiation by establishing opsin and peripherin expression by immunocytochemistry and Western blot. Apoptosis was inhibited with the caspase pan-inhibitor ZVADFMK and evaluated by TUNEL assay, propidium iodide/annexin V and DAPI labeling. Preservation of mitochondrial membrane potential was evaluated. Results. C1P enhanced BrdU uptake and increased mitosis in retinal progenitors. C1P addition advanced photoreceptor differentiation, enhancing opsin and peripherin expression and stimulating development of apical processes, in which these proteins were concentrated. In the absence of their trophic factors, photoreceptors degenerate after 4 days in vitro and at day 6 almost 50% of photoreceptors were apoptotic; C1P decreased photoreceptor apoptosis, reducing this percentage by half. Inhibiting caspase activity reduced photoreceptor apoptosis in controls but did not increase opsin expression, implying C1P has separate effects on differentiation and survival. Conclusions. These results suggest for the first time that C1P is a novel mediator that plays multiple functions in photoreceptors, initially regulating their proliferation and then promoting their survival and differentiation.
Fil: Miranda, Gisela Edit. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Abrahan, Carolina Elizabeth. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Agnolazza, Daniela Luciana. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Politi, Luis Enrique. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Rotstein, Nora Patricia. Consejo Nacional de Investigaciones Cientificas y Técnicas. Centro Científico Tecnológico Bahia Blanca. Instituto de Investigaciones Bioquímicas Bahia Blanca (i); Argentina. Universidad Nacional del Sur; Argentina
description Purpose. Simple sphingolipids control crucial cellular processes in several cell types. We demonstrated that sphingolipids such as ceramide, sphingosine and sphingosine-1-phosphate are key mediators in the regulation of survival, differentiation and proliferation of retina photoreceptors. Ceramide-1-phosphate (C1P) regulates growth and survival in several cell types; however, little is known concerning its functions in the retina. We here explored whether C1P also participated in controlling photoreceptor development. Methods. Rat retina neuronal cultures were supplemented with 1-10 µM C1P. Proliferation was determined by evaluating 5-bromo-2-deoxyuridine (BrdU) uptake and number of mitotic figures and differentiation by establishing opsin and peripherin expression by immunocytochemistry and Western blot. Apoptosis was inhibited with the caspase pan-inhibitor ZVADFMK and evaluated by TUNEL assay, propidium iodide/annexin V and DAPI labeling. Preservation of mitochondrial membrane potential was evaluated. Results. C1P enhanced BrdU uptake and increased mitosis in retinal progenitors. C1P addition advanced photoreceptor differentiation, enhancing opsin and peripherin expression and stimulating development of apical processes, in which these proteins were concentrated. In the absence of their trophic factors, photoreceptors degenerate after 4 days in vitro and at day 6 almost 50% of photoreceptors were apoptotic; C1P decreased photoreceptor apoptosis, reducing this percentage by half. Inhibiting caspase activity reduced photoreceptor apoptosis in controls but did not increase opsin expression, implying C1P has separate effects on differentiation and survival. Conclusions. These results suggest for the first time that C1P is a novel mediator that plays multiple functions in photoreceptors, initially regulating their proliferation and then promoting their survival and differentiation.
publishDate 2011
dc.date.none.fl_str_mv 2011-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/6465
Miranda, Gisela Edit; Abrahan, Carolina Elizabeth; Agnolazza, Daniela Luciana; Politi, Luis Enrique; Rotstein, Nora Patricia; Ceramide-1-phosphate: a new mediator of survival and development in retina photoreceptors; Association For Research In Vision And Ophthalmology; Investigative Ophthalmology & Visual Science; 52; 9; 8-2011; 6580-6588
0146-0404
url http://hdl.handle.net/11336/6465
identifier_str_mv Miranda, Gisela Edit; Abrahan, Carolina Elizabeth; Agnolazza, Daniela Luciana; Politi, Luis Enrique; Rotstein, Nora Patricia; Ceramide-1-phosphate: a new mediator of survival and development in retina photoreceptors; Association For Research In Vision And Ophthalmology; Investigative Ophthalmology & Visual Science; 52; 9; 8-2011; 6580-6588
0146-0404
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/pmid/21724910
info:eu-repo/semantics/altIdentifier/doi/10.1167/iovs.10-7065
info:eu-repo/semantics/altIdentifier/url/http://iovs.arvojournals.org/article.aspx?articleid=2187942
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Association For Research In Vision And Ophthalmology
publisher.none.fl_str_mv Association For Research In Vision And Ophthalmology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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