Structural stability of Sclerotium rolfsii ATCC 201126 β-glucan with fermentation time: a chemical, infrared spectroscopic and enzymatic approach

Autores
Fariña, Julia Ines; Viñarta, Silvana Carolina; Cattaneo, Mauricio; Figueroa L.I.C.
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sclerotium rolfsii ATCC 201126 exopolysaccharides (EPSs) recovered at 48 h (EPS I) and 72 h (EPS II) of fermentation, with differences in rheological parameters, hydrogel topography, salt tolerance, antisyneresis, emulsifying and suspending properties, were subjected to a polyphasic characterization in order to detect structural divergences. Methods and Results: Fermenter-scale production led to productivity (Pr) and yield (YP ⁄ C) values higher at 48 h (Pr = 0Æ542 g l)1 h)1 ; YP ⁄ C = 0Æ74) than at 72 h (Pr = 0Æ336 g l)1 h)1 ; YP ⁄ C = 0Æ50). Both EPSs were neutral glucosehomopolysaccharides with a b-(1,3)-glycosidic backbone and single b-(1,6)- glucopyranosyl sidechains regularly attached every three residues in the main chain, as revealed by chemical analyses. The infra-red diagnostic peak at 890 cm)1 confirmed b-glycosidic linkages, while gentiobiose released by b-(1,3)-glucanases confirmed single b-1,6-glycosidic branching for both EPSs. Conclusions: The true modular repeating unit of S. rolfsii ATCC 201126 scleroglucan could be resolved. Structural stability was corroborated and no structural differences could be detected as to account for the variations in EPSs behaviour. Significance and Impact of the Study: Recovery of S. rolfsii ATCC 201126 scleroglucan at 48 h might be considered based on better fermentation kinetic parameters and no detrimental effects on EPS structural features.
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Viñarta, Silvana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Cattaneo, Mauricio. Universidad Nacional de Tucumán; Argentina
Fil: Figueroa L.I.C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Materia
Sclerotium Rolfsii
Scleroglucan
Fermentation
Structure
Enzymatic Digestion
Ft-Ir
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/42040
Acceder
id CONICETDig_64096b033ad7f1c32bf31a069bd401fc
oai_identifier_str oai:ri.conicet.gov.ar:11336/42040
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Structural stability of Sclerotium rolfsii ATCC 201126 β-glucan with fermentation time: a chemical, infrared spectroscopic and enzymatic approachFariña, Julia InesViñarta, Silvana CarolinaCattaneo, MauricioFigueroa L.I.C.Sclerotium RolfsiiScleroglucanFermentationStructureEnzymatic DigestionFt-Irhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Sclerotium rolfsii ATCC 201126 exopolysaccharides (EPSs) recovered at 48 h (EPS I) and 72 h (EPS II) of fermentation, with differences in rheological parameters, hydrogel topography, salt tolerance, antisyneresis, emulsifying and suspending properties, were subjected to a polyphasic characterization in order to detect structural divergences. Methods and Results: Fermenter-scale production led to productivity (Pr) and yield (YP ⁄ C) values higher at 48 h (Pr = 0Æ542 g l)1 h)1 ; YP ⁄ C = 0Æ74) than at 72 h (Pr = 0Æ336 g l)1 h)1 ; YP ⁄ C = 0Æ50). Both EPSs were neutral glucosehomopolysaccharides with a b-(1,3)-glycosidic backbone and single b-(1,6)- glucopyranosyl sidechains regularly attached every three residues in the main chain, as revealed by chemical analyses. The infra-red diagnostic peak at 890 cm)1 confirmed b-glycosidic linkages, while gentiobiose released by b-(1,3)-glucanases confirmed single b-1,6-glycosidic branching for both EPSs. Conclusions: The true modular repeating unit of S. rolfsii ATCC 201126 scleroglucan could be resolved. Structural stability was corroborated and no structural differences could be detected as to account for the variations in EPSs behaviour. Significance and Impact of the Study: Recovery of S. rolfsii ATCC 201126 scleroglucan at 48 h might be considered based on better fermentation kinetic parameters and no detrimental effects on EPS structural features.Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Viñarta, Silvana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Cattaneo, Mauricio. Universidad Nacional de Tucumán; ArgentinaFil: Figueroa L.I.C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaWiley Blackwell Publishing, Inc2009-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/42040Fariña, Julia Ines; Viñarta, Silvana Carolina; Cattaneo, Mauricio; Figueroa L.I.C.; Structural stability of Sclerotium rolfsii ATCC 201126 β-glucan with fermentation time: a chemical, infrared spectroscopic and enzymatic approach; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 106; 1; 1-2009; 221-2321364-5072CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2008.03995.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2672.2008.03995.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:47:48Zoai:ri.conicet.gov.ar:11336/42040instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:47:49.283CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Structural stability of Sclerotium rolfsii ATCC 201126 β-glucan with fermentation time: a chemical, infrared spectroscopic and enzymatic approach
title Structural stability of Sclerotium rolfsii ATCC 201126 β-glucan with fermentation time: a chemical, infrared spectroscopic and enzymatic approach
spellingShingle Structural stability of Sclerotium rolfsii ATCC 201126 β-glucan with fermentation time: a chemical, infrared spectroscopic and enzymatic approach
Fariña, Julia Ines
Sclerotium Rolfsii
Scleroglucan
Fermentation
Structure
Enzymatic Digestion
Ft-Ir
title_short Structural stability of Sclerotium rolfsii ATCC 201126 β-glucan with fermentation time: a chemical, infrared spectroscopic and enzymatic approach
title_full Structural stability of Sclerotium rolfsii ATCC 201126 β-glucan with fermentation time: a chemical, infrared spectroscopic and enzymatic approach
title_fullStr Structural stability of Sclerotium rolfsii ATCC 201126 β-glucan with fermentation time: a chemical, infrared spectroscopic and enzymatic approach
title_full_unstemmed Structural stability of Sclerotium rolfsii ATCC 201126 β-glucan with fermentation time: a chemical, infrared spectroscopic and enzymatic approach
title_sort Structural stability of Sclerotium rolfsii ATCC 201126 β-glucan with fermentation time: a chemical, infrared spectroscopic and enzymatic approach
dc.creator.none.fl_str_mv Fariña, Julia Ines
Viñarta, Silvana Carolina
Cattaneo, Mauricio
Figueroa L.I.C.
author Fariña, Julia Ines
author_facet Fariña, Julia Ines
Viñarta, Silvana Carolina
Cattaneo, Mauricio
Figueroa L.I.C.
author_role author
author2 Viñarta, Silvana Carolina
Cattaneo, Mauricio
Figueroa L.I.C.
author2_role author
author
author
dc.subject.none.fl_str_mv Sclerotium Rolfsii
Scleroglucan
Fermentation
Structure
Enzymatic Digestion
Ft-Ir
topic Sclerotium Rolfsii
Scleroglucan
Fermentation
Structure
Enzymatic Digestion
Ft-Ir
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Sclerotium rolfsii ATCC 201126 exopolysaccharides (EPSs) recovered at 48 h (EPS I) and 72 h (EPS II) of fermentation, with differences in rheological parameters, hydrogel topography, salt tolerance, antisyneresis, emulsifying and suspending properties, were subjected to a polyphasic characterization in order to detect structural divergences. Methods and Results: Fermenter-scale production led to productivity (Pr) and yield (YP ⁄ C) values higher at 48 h (Pr = 0Æ542 g l)1 h)1 ; YP ⁄ C = 0Æ74) than at 72 h (Pr = 0Æ336 g l)1 h)1 ; YP ⁄ C = 0Æ50). Both EPSs were neutral glucosehomopolysaccharides with a b-(1,3)-glycosidic backbone and single b-(1,6)- glucopyranosyl sidechains regularly attached every three residues in the main chain, as revealed by chemical analyses. The infra-red diagnostic peak at 890 cm)1 confirmed b-glycosidic linkages, while gentiobiose released by b-(1,3)-glucanases confirmed single b-1,6-glycosidic branching for both EPSs. Conclusions: The true modular repeating unit of S. rolfsii ATCC 201126 scleroglucan could be resolved. Structural stability was corroborated and no structural differences could be detected as to account for the variations in EPSs behaviour. Significance and Impact of the Study: Recovery of S. rolfsii ATCC 201126 scleroglucan at 48 h might be considered based on better fermentation kinetic parameters and no detrimental effects on EPS structural features.
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Viñarta, Silvana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Cattaneo, Mauricio. Universidad Nacional de Tucumán; Argentina
Fil: Figueroa L.I.C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
description Sclerotium rolfsii ATCC 201126 exopolysaccharides (EPSs) recovered at 48 h (EPS I) and 72 h (EPS II) of fermentation, with differences in rheological parameters, hydrogel topography, salt tolerance, antisyneresis, emulsifying and suspending properties, were subjected to a polyphasic characterization in order to detect structural divergences. Methods and Results: Fermenter-scale production led to productivity (Pr) and yield (YP ⁄ C) values higher at 48 h (Pr = 0Æ542 g l)1 h)1 ; YP ⁄ C = 0Æ74) than at 72 h (Pr = 0Æ336 g l)1 h)1 ; YP ⁄ C = 0Æ50). Both EPSs were neutral glucosehomopolysaccharides with a b-(1,3)-glycosidic backbone and single b-(1,6)- glucopyranosyl sidechains regularly attached every three residues in the main chain, as revealed by chemical analyses. The infra-red diagnostic peak at 890 cm)1 confirmed b-glycosidic linkages, while gentiobiose released by b-(1,3)-glucanases confirmed single b-1,6-glycosidic branching for both EPSs. Conclusions: The true modular repeating unit of S. rolfsii ATCC 201126 scleroglucan could be resolved. Structural stability was corroborated and no structural differences could be detected as to account for the variations in EPSs behaviour. Significance and Impact of the Study: Recovery of S. rolfsii ATCC 201126 scleroglucan at 48 h might be considered based on better fermentation kinetic parameters and no detrimental effects on EPS structural features.
publishDate 2009
dc.date.none.fl_str_mv 2009-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/42040
Fariña, Julia Ines; Viñarta, Silvana Carolina; Cattaneo, Mauricio; Figueroa L.I.C.; Structural stability of Sclerotium rolfsii ATCC 201126 β-glucan with fermentation time: a chemical, infrared spectroscopic and enzymatic approach; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 106; 1; 1-2009; 221-232
1364-5072
CONICET Digital
CONICET
url http://hdl.handle.net/11336/42040
identifier_str_mv Fariña, Julia Ines; Viñarta, Silvana Carolina; Cattaneo, Mauricio; Figueroa L.I.C.; Structural stability of Sclerotium rolfsii ATCC 201126 β-glucan with fermentation time: a chemical, infrared spectroscopic and enzymatic approach; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 106; 1; 1-2009; 221-232
1364-5072
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2008.03995.x
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2672.2008.03995.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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