Novel linear megaplasmid from Brevibacterium sp. isolated from extreme environment

Autores
Dib, Julian Rafael; Wagenknecht, Martin; Hill, Russell T.; Farias, Maria Eugenia; Meinhardt, Friedhelm
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Brevibacterium sp. Ap13, isolated from flamingo's feces in Laguna Aparejos, a high-altitude lake located at approximately 4,200 m in the northwest of Argentina was previously found to be resistant to multiple antibiotics, and was therefore screened for plasmids that may be implicated in antibiotic resistance. Brevibacterium sp. Ap13 was found to contain two plasmids of approximately 87 and 436 kb, designated pAP13 and pAP13c, respectively. Only pAP13 was stably maintained and was extensively characterized by pulsed-field gel electrophoresis to reveal that this plasmid is linear and likely has covalently linked terminal proteins associated with its 5' ends. This is the first report of a linear plasmid in the genus Brevibacterium and may provide a new tool for genetic manipulation of this commercially important genus.
Fil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Wagenknecht, Martin. Westfalische Wilhelms Universitat; Alemania
Fil: Hill, Russell T.. University of Maryland; Estados Unidos
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Meinhardt, Friedhelm. Westfalische Wilhelms Universitat; Alemania
Materia
Linear Plasmid
Terminal Protein
Brevibacterium
Argentinean High-Altitude Lakes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41509
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Novel linear megaplasmid from Brevibacterium sp. isolated from extreme environmentDib, Julian RafaelWagenknecht, MartinHill, Russell T.Farias, Maria EugeniaMeinhardt, FriedhelmLinear PlasmidTerminal ProteinBrevibacteriumArgentinean High-Altitude Lakeshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Brevibacterium sp. Ap13, isolated from flamingo's feces in Laguna Aparejos, a high-altitude lake located at approximately 4,200 m in the northwest of Argentina was previously found to be resistant to multiple antibiotics, and was therefore screened for plasmids that may be implicated in antibiotic resistance. Brevibacterium sp. Ap13 was found to contain two plasmids of approximately 87 and 436 kb, designated pAP13 and pAP13c, respectively. Only pAP13 was stably maintained and was extensively characterized by pulsed-field gel electrophoresis to reveal that this plasmid is linear and likely has covalently linked terminal proteins associated with its 5' ends. This is the first report of a linear plasmid in the genus Brevibacterium and may provide a new tool for genetic manipulation of this commercially important genus.Fil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Wagenknecht, Martin. Westfalische Wilhelms Universitat; AlemaniaFil: Hill, Russell T.. University of Maryland; Estados UnidosFil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Meinhardt, Friedhelm. Westfalische Wilhelms Universitat; AlemaniaWiley VCH Verlag2010-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41509Dib, Julian Rafael; Wagenknecht, Martin; Hill, Russell T.; Farias, Maria Eugenia; Meinhardt, Friedhelm; Novel linear megaplasmid from Brevibacterium sp. isolated from extreme environment; Wiley VCH Verlag; Journal of Basic Microbiology; 50; 3; 5-2010; 280-2840233-111XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/jobm.200900332info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jobm.200900332info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:47Zoai:ri.conicet.gov.ar:11336/41509instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:51:48.149CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Novel linear megaplasmid from Brevibacterium sp. isolated from extreme environment
title Novel linear megaplasmid from Brevibacterium sp. isolated from extreme environment
spellingShingle Novel linear megaplasmid from Brevibacterium sp. isolated from extreme environment
Dib, Julian Rafael
Linear Plasmid
Terminal Protein
Brevibacterium
Argentinean High-Altitude Lakes
title_short Novel linear megaplasmid from Brevibacterium sp. isolated from extreme environment
title_full Novel linear megaplasmid from Brevibacterium sp. isolated from extreme environment
title_fullStr Novel linear megaplasmid from Brevibacterium sp. isolated from extreme environment
title_full_unstemmed Novel linear megaplasmid from Brevibacterium sp. isolated from extreme environment
title_sort Novel linear megaplasmid from Brevibacterium sp. isolated from extreme environment
dc.creator.none.fl_str_mv Dib, Julian Rafael
Wagenknecht, Martin
Hill, Russell T.
Farias, Maria Eugenia
Meinhardt, Friedhelm
author Dib, Julian Rafael
author_facet Dib, Julian Rafael
Wagenknecht, Martin
Hill, Russell T.
Farias, Maria Eugenia
Meinhardt, Friedhelm
author_role author
author2 Wagenknecht, Martin
Hill, Russell T.
Farias, Maria Eugenia
Meinhardt, Friedhelm
author2_role author
author
author
author
dc.subject.none.fl_str_mv Linear Plasmid
Terminal Protein
Brevibacterium
Argentinean High-Altitude Lakes
topic Linear Plasmid
Terminal Protein
Brevibacterium
Argentinean High-Altitude Lakes
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Brevibacterium sp. Ap13, isolated from flamingo's feces in Laguna Aparejos, a high-altitude lake located at approximately 4,200 m in the northwest of Argentina was previously found to be resistant to multiple antibiotics, and was therefore screened for plasmids that may be implicated in antibiotic resistance. Brevibacterium sp. Ap13 was found to contain two plasmids of approximately 87 and 436 kb, designated pAP13 and pAP13c, respectively. Only pAP13 was stably maintained and was extensively characterized by pulsed-field gel electrophoresis to reveal that this plasmid is linear and likely has covalently linked terminal proteins associated with its 5' ends. This is the first report of a linear plasmid in the genus Brevibacterium and may provide a new tool for genetic manipulation of this commercially important genus.
Fil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Wagenknecht, Martin. Westfalische Wilhelms Universitat; Alemania
Fil: Hill, Russell T.. University of Maryland; Estados Unidos
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Meinhardt, Friedhelm. Westfalische Wilhelms Universitat; Alemania
description Brevibacterium sp. Ap13, isolated from flamingo's feces in Laguna Aparejos, a high-altitude lake located at approximately 4,200 m in the northwest of Argentina was previously found to be resistant to multiple antibiotics, and was therefore screened for plasmids that may be implicated in antibiotic resistance. Brevibacterium sp. Ap13 was found to contain two plasmids of approximately 87 and 436 kb, designated pAP13 and pAP13c, respectively. Only pAP13 was stably maintained and was extensively characterized by pulsed-field gel electrophoresis to reveal that this plasmid is linear and likely has covalently linked terminal proteins associated with its 5' ends. This is the first report of a linear plasmid in the genus Brevibacterium and may provide a new tool for genetic manipulation of this commercially important genus.
publishDate 2010
dc.date.none.fl_str_mv 2010-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41509
Dib, Julian Rafael; Wagenknecht, Martin; Hill, Russell T.; Farias, Maria Eugenia; Meinhardt, Friedhelm; Novel linear megaplasmid from Brevibacterium sp. isolated from extreme environment; Wiley VCH Verlag; Journal of Basic Microbiology; 50; 3; 5-2010; 280-284
0233-111X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41509
identifier_str_mv Dib, Julian Rafael; Wagenknecht, Martin; Hill, Russell T.; Farias, Maria Eugenia; Meinhardt, Friedhelm; Novel linear megaplasmid from Brevibacterium sp. isolated from extreme environment; Wiley VCH Verlag; Journal of Basic Microbiology; 50; 3; 5-2010; 280-284
0233-111X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/jobm.200900332
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jobm.200900332
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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