Micropropagation of wild species of the genus Asparagus L. and their interspecific hybrids with cultivated A. officinalis L., and verification of genetic stability using EST-SSRs

Autores
Regalado González, Jose Javier; Carmona Martín, E.; Castro, P.; Moreno, R.; Gil, J.; Encina, C. L.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An efficient micropropagation method forasparagus species was developed in this study. The methodallows the fast cloning of the elite genotypes from differentasparagus species and the interspecific hybrids obtainedfrom these species. Rhizome bud explants were disinfectedusing 3 g l-1 of benomyl and 20 g l-1 of sodium hypochlorite.Then, they were cultured on Asparagus RhizomeBud Medium 3 (ARBM-3) consisting in modified Murashigeand Skoog medium with salts with EDDHA-Fe(85.7 mg l-1) instead of EDTA-Fe and vitamins, supplementedwith 0.3 mg l-1 NAA, 0.1 mg l-1 KIN, 2 mg l-1ancymidol and 6 % sucrose. Results showed that themethod developed produced high disinfection rates(70?95 %). More than 70 % of the explants developedshoots and the rooting rate on ARBM-3 medium was30?45 %. The rooting rate increased to 60?85 % when theunrooted shoots were subjected to an additional cycle ofrooting, reaching 100 % after two cycles of rooting. Themultiplication was achieved through mechanical divisionof rooted shoot clusters growing in ARBM-3. The acclimatizationrate of the micropropagated plantlets was higherthan 90 %. The micropopagated plantlets were screened forsomaclonal variation using 12 expressed sequence tagderived simple sequence repeat markers. The results confirmedthe character ??true to type?? of the plantlets, indicatingthat the method developed in this study can be usedto successfully micropropagate asparagus species.
Fil: Regalado González, Jose Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Superior de Investigaciones Científicas; España
Fil: Carmona Martín, E.. Consejo Superior de Investigaciones Científicas; España
Fil: Castro, P.. Genetic Improvement of Fruits and Vegetables Laboratory; Estados Unidos
Fil: Moreno, R.. Universidad de Córdoba; España
Fil: Gil, J.. Universidad de Córdoba; España
Fil: Encina, C. L.. Consejo Superior de Investigaciones Científicas; España
Materia
Rhizome Buds
Rooting
Molecular Markers
Somaclonal Variation
Asparagus Brachyphyllus
Asparagus Pseudoscaber
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/43715
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/43715
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Micropropagation of wild species of the genus Asparagus L. and their interspecific hybrids with cultivated A. officinalis L., and verification of genetic stability using EST-SSRsRegalado González, Jose JavierCarmona Martín, E.Castro, P.Moreno, R.Gil, J.Encina, C. L.Rhizome BudsRootingMolecular MarkersSomaclonal VariationAsparagus BrachyphyllusAsparagus Pseudoscaberhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1An efficient micropropagation method forasparagus species was developed in this study. The methodallows the fast cloning of the elite genotypes from differentasparagus species and the interspecific hybrids obtainedfrom these species. Rhizome bud explants were disinfectedusing 3 g l-1 of benomyl and 20 g l-1 of sodium hypochlorite.Then, they were cultured on Asparagus RhizomeBud Medium 3 (ARBM-3) consisting in modified Murashigeand Skoog medium with salts with EDDHA-Fe(85.7 mg l-1) instead of EDTA-Fe and vitamins, supplementedwith 0.3 mg l-1 NAA, 0.1 mg l-1 KIN, 2 mg l-1ancymidol and 6 % sucrose. Results showed that themethod developed produced high disinfection rates(70?95 %). More than 70 % of the explants developedshoots and the rooting rate on ARBM-3 medium was30?45 %. The rooting rate increased to 60?85 % when theunrooted shoots were subjected to an additional cycle ofrooting, reaching 100 % after two cycles of rooting. Themultiplication was achieved through mechanical divisionof rooted shoot clusters growing in ARBM-3. The acclimatizationrate of the micropropagated plantlets was higherthan 90 %. The micropopagated plantlets were screened forsomaclonal variation using 12 expressed sequence tagderived simple sequence repeat markers. The results confirmedthe character ??true to type?? of the plantlets, indicatingthat the method developed in this study can be usedto successfully micropropagate asparagus species.Fil: Regalado González, Jose Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Superior de Investigaciones Científicas; EspañaFil: Carmona Martín, E.. Consejo Superior de Investigaciones Científicas; EspañaFil: Castro, P.. Genetic Improvement of Fruits and Vegetables Laboratory; Estados UnidosFil: Moreno, R.. Universidad de Córdoba; EspañaFil: Gil, J.. Universidad de Córdoba; EspañaFil: Encina, C. L.. Consejo Superior de Investigaciones Científicas; EspañaSpringer2015-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/43715Regalado González, Jose Javier; Carmona Martín, E.; Castro, P.; Moreno, R.; Gil, J.; et al.; Micropropagation of wild species of the genus Asparagus L. and their interspecific hybrids with cultivated A. officinalis L., and verification of genetic stability using EST-SSRs; Springer; Plant Cell, Tissue and Organ Culture; 121; 2; 5-2015; 501-5100167-68571573-5044CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11240-015-0720-8info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11240-015-0720-8info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:13:53Zoai:ri.conicet.gov.ar:11336/43715instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:13:54.068CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Micropropagation of wild species of the genus Asparagus L. and their interspecific hybrids with cultivated A. officinalis L., and verification of genetic stability using EST-SSRs
title Micropropagation of wild species of the genus Asparagus L. and their interspecific hybrids with cultivated A. officinalis L., and verification of genetic stability using EST-SSRs
spellingShingle Micropropagation of wild species of the genus Asparagus L. and their interspecific hybrids with cultivated A. officinalis L., and verification of genetic stability using EST-SSRs
Regalado González, Jose Javier
Rhizome Buds
Rooting
Molecular Markers
Somaclonal Variation
Asparagus Brachyphyllus
Asparagus Pseudoscaber
title_short Micropropagation of wild species of the genus Asparagus L. and their interspecific hybrids with cultivated A. officinalis L., and verification of genetic stability using EST-SSRs
title_full Micropropagation of wild species of the genus Asparagus L. and their interspecific hybrids with cultivated A. officinalis L., and verification of genetic stability using EST-SSRs
title_fullStr Micropropagation of wild species of the genus Asparagus L. and their interspecific hybrids with cultivated A. officinalis L., and verification of genetic stability using EST-SSRs
title_full_unstemmed Micropropagation of wild species of the genus Asparagus L. and their interspecific hybrids with cultivated A. officinalis L., and verification of genetic stability using EST-SSRs
title_sort Micropropagation of wild species of the genus Asparagus L. and their interspecific hybrids with cultivated A. officinalis L., and verification of genetic stability using EST-SSRs
dc.creator.none.fl_str_mv Regalado González, Jose Javier
Carmona Martín, E.
Castro, P.
Moreno, R.
Gil, J.
Encina, C. L.
author Regalado González, Jose Javier
author_facet Regalado González, Jose Javier
Carmona Martín, E.
Castro, P.
Moreno, R.
Gil, J.
Encina, C. L.
author_role author
author2 Carmona Martín, E.
Castro, P.
Moreno, R.
Gil, J.
Encina, C. L.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Rhizome Buds
Rooting
Molecular Markers
Somaclonal Variation
Asparagus Brachyphyllus
Asparagus Pseudoscaber
topic Rhizome Buds
Rooting
Molecular Markers
Somaclonal Variation
Asparagus Brachyphyllus
Asparagus Pseudoscaber
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv An efficient micropropagation method forasparagus species was developed in this study. The methodallows the fast cloning of the elite genotypes from differentasparagus species and the interspecific hybrids obtainedfrom these species. Rhizome bud explants were disinfectedusing 3 g l-1 of benomyl and 20 g l-1 of sodium hypochlorite.Then, they were cultured on Asparagus RhizomeBud Medium 3 (ARBM-3) consisting in modified Murashigeand Skoog medium with salts with EDDHA-Fe(85.7 mg l-1) instead of EDTA-Fe and vitamins, supplementedwith 0.3 mg l-1 NAA, 0.1 mg l-1 KIN, 2 mg l-1ancymidol and 6 % sucrose. Results showed that themethod developed produced high disinfection rates(70?95 %). More than 70 % of the explants developedshoots and the rooting rate on ARBM-3 medium was30?45 %. The rooting rate increased to 60?85 % when theunrooted shoots were subjected to an additional cycle ofrooting, reaching 100 % after two cycles of rooting. Themultiplication was achieved through mechanical divisionof rooted shoot clusters growing in ARBM-3. The acclimatizationrate of the micropropagated plantlets was higherthan 90 %. The micropopagated plantlets were screened forsomaclonal variation using 12 expressed sequence tagderived simple sequence repeat markers. The results confirmedthe character ??true to type?? of the plantlets, indicatingthat the method developed in this study can be usedto successfully micropropagate asparagus species.
Fil: Regalado González, Jose Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Superior de Investigaciones Científicas; España
Fil: Carmona Martín, E.. Consejo Superior de Investigaciones Científicas; España
Fil: Castro, P.. Genetic Improvement of Fruits and Vegetables Laboratory; Estados Unidos
Fil: Moreno, R.. Universidad de Córdoba; España
Fil: Gil, J.. Universidad de Córdoba; España
Fil: Encina, C. L.. Consejo Superior de Investigaciones Científicas; España
description An efficient micropropagation method forasparagus species was developed in this study. The methodallows the fast cloning of the elite genotypes from differentasparagus species and the interspecific hybrids obtainedfrom these species. Rhizome bud explants were disinfectedusing 3 g l-1 of benomyl and 20 g l-1 of sodium hypochlorite.Then, they were cultured on Asparagus RhizomeBud Medium 3 (ARBM-3) consisting in modified Murashigeand Skoog medium with salts with EDDHA-Fe(85.7 mg l-1) instead of EDTA-Fe and vitamins, supplementedwith 0.3 mg l-1 NAA, 0.1 mg l-1 KIN, 2 mg l-1ancymidol and 6 % sucrose. Results showed that themethod developed produced high disinfection rates(70?95 %). More than 70 % of the explants developedshoots and the rooting rate on ARBM-3 medium was30?45 %. The rooting rate increased to 60?85 % when theunrooted shoots were subjected to an additional cycle ofrooting, reaching 100 % after two cycles of rooting. Themultiplication was achieved through mechanical divisionof rooted shoot clusters growing in ARBM-3. The acclimatizationrate of the micropropagated plantlets was higherthan 90 %. The micropopagated plantlets were screened forsomaclonal variation using 12 expressed sequence tagderived simple sequence repeat markers. The results confirmedthe character ??true to type?? of the plantlets, indicatingthat the method developed in this study can be usedto successfully micropropagate asparagus species.
publishDate 2015
dc.date.none.fl_str_mv 2015-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/43715
Regalado González, Jose Javier; Carmona Martín, E.; Castro, P.; Moreno, R.; Gil, J.; et al.; Micropropagation of wild species of the genus Asparagus L. and their interspecific hybrids with cultivated A. officinalis L., and verification of genetic stability using EST-SSRs; Springer; Plant Cell, Tissue and Organ Culture; 121; 2; 5-2015; 501-510
0167-6857
1573-5044
CONICET Digital
CONICET
url http://hdl.handle.net/11336/43715
identifier_str_mv Regalado González, Jose Javier; Carmona Martín, E.; Castro, P.; Moreno, R.; Gil, J.; et al.; Micropropagation of wild species of the genus Asparagus L. and their interspecific hybrids with cultivated A. officinalis L., and verification of genetic stability using EST-SSRs; Springer; Plant Cell, Tissue and Organ Culture; 121; 2; 5-2015; 501-510
0167-6857
1573-5044
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s11240-015-0720-8
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11240-015-0720-8
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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