In vitro induction of autooctoploid asparagus genotypes

Autores
Carmona Martín, E.; Regalado González, Jose Javier; Raghavan, L.; Encina, C. L.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Octoploid genotypes of Asparagus officinalis L. cv. ‘Morado de Huetor’, a tetraploid Spanish landrace, were successfully induced by treating in vitro rhizome buds explants with colchicine. Pulses during 24 h with different concentrations of colchicine are able to induce polyploid plants, colchicine applied at 0.1 % induce a 7 % of octoploid genotypes with a stable ploidy level and a 3.5 % of mixoploid genotypes. The maximum rate of mixoploid genotypes (26 %) was obtained with 0.3 % colchicine. The plant survival and rooting rates of explants decrease for increasing doses of colchicine. All octoploid genotypes were micropropagated, rooted, transplanted and successfully acclimatized (100 %) in a glasshouse. The four octoploid plants recovered show significantly better agronomical parameters such as spear diameter, canopy area and shoot length than the original tetraploid plants and histological studies confirm the size increase of octoploid cells respect the original tetraploid cells.
Fil: Carmona Martín, E.. Instituto de Hortofruticultura Subtropical y Mediterránea; España
Fil: Regalado González, Jose Javier. Instituto de Hortofruticultura Subtropical y Mediterránea; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Raghavan, L.. Sugarcane Breeding Institute; India
Fil: Encina, C. L.. Instituto de Hortofruticultura Subtropical y Mediterránea; España
Materia
Rhizome Bud
Colchicine
Ploidy
Chromosome
Flow Cytometry
Chlorophyll
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/42682
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/42682
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vitro induction of autooctoploid asparagus genotypesCarmona Martín, E.Regalado González, Jose JavierRaghavan, L.Encina, C. L.Rhizome BudColchicinePloidyChromosomeFlow CytometryChlorophyllhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Octoploid genotypes of Asparagus officinalis L. cv. ‘Morado de Huetor’, a tetraploid Spanish landrace, were successfully induced by treating in vitro rhizome buds explants with colchicine. Pulses during 24 h with different concentrations of colchicine are able to induce polyploid plants, colchicine applied at 0.1 % induce a 7 % of octoploid genotypes with a stable ploidy level and a 3.5 % of mixoploid genotypes. The maximum rate of mixoploid genotypes (26 %) was obtained with 0.3 % colchicine. The plant survival and rooting rates of explants decrease for increasing doses of colchicine. All octoploid genotypes were micropropagated, rooted, transplanted and successfully acclimatized (100 %) in a glasshouse. The four octoploid plants recovered show significantly better agronomical parameters such as spear diameter, canopy area and shoot length than the original tetraploid plants and histological studies confirm the size increase of octoploid cells respect the original tetraploid cells.Fil: Carmona Martín, E.. Instituto de Hortofruticultura Subtropical y Mediterránea; EspañaFil: Regalado González, Jose Javier. Instituto de Hortofruticultura Subtropical y Mediterránea; España. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Raghavan, L.. Sugarcane Breeding Institute; IndiaFil: Encina, C. L.. Instituto de Hortofruticultura Subtropical y Mediterránea; EspañaSpringer2014-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/42682Carmona Martín, E.; Regalado González, Jose Javier; Raghavan, L.; Encina, C. L.; In vitro induction of autooctoploid asparagus genotypes; Springer; Plant Cell, Tissue and Organ Culture; 121; 1; 12-2014; 249-2540167-68571573-5044CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11240-014-0680-4info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11240-014-0680-4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:10:51Zoai:ri.conicet.gov.ar:11336/42682instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:51.742CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro induction of autooctoploid asparagus genotypes
title In vitro induction of autooctoploid asparagus genotypes
spellingShingle In vitro induction of autooctoploid asparagus genotypes
Carmona Martín, E.
Rhizome Bud
Colchicine
Ploidy
Chromosome
Flow Cytometry
Chlorophyll
title_short In vitro induction of autooctoploid asparagus genotypes
title_full In vitro induction of autooctoploid asparagus genotypes
title_fullStr In vitro induction of autooctoploid asparagus genotypes
title_full_unstemmed In vitro induction of autooctoploid asparagus genotypes
title_sort In vitro induction of autooctoploid asparagus genotypes
dc.creator.none.fl_str_mv Carmona Martín, E.
Regalado González, Jose Javier
Raghavan, L.
Encina, C. L.
author Carmona Martín, E.
author_facet Carmona Martín, E.
Regalado González, Jose Javier
Raghavan, L.
Encina, C. L.
author_role author
author2 Regalado González, Jose Javier
Raghavan, L.
Encina, C. L.
author2_role author
author
author
dc.subject.none.fl_str_mv Rhizome Bud
Colchicine
Ploidy
Chromosome
Flow Cytometry
Chlorophyll
topic Rhizome Bud
Colchicine
Ploidy
Chromosome
Flow Cytometry
Chlorophyll
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Octoploid genotypes of Asparagus officinalis L. cv. ‘Morado de Huetor’, a tetraploid Spanish landrace, were successfully induced by treating in vitro rhizome buds explants with colchicine. Pulses during 24 h with different concentrations of colchicine are able to induce polyploid plants, colchicine applied at 0.1 % induce a 7 % of octoploid genotypes with a stable ploidy level and a 3.5 % of mixoploid genotypes. The maximum rate of mixoploid genotypes (26 %) was obtained with 0.3 % colchicine. The plant survival and rooting rates of explants decrease for increasing doses of colchicine. All octoploid genotypes were micropropagated, rooted, transplanted and successfully acclimatized (100 %) in a glasshouse. The four octoploid plants recovered show significantly better agronomical parameters such as spear diameter, canopy area and shoot length than the original tetraploid plants and histological studies confirm the size increase of octoploid cells respect the original tetraploid cells.
Fil: Carmona Martín, E.. Instituto de Hortofruticultura Subtropical y Mediterránea; España
Fil: Regalado González, Jose Javier. Instituto de Hortofruticultura Subtropical y Mediterránea; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Raghavan, L.. Sugarcane Breeding Institute; India
Fil: Encina, C. L.. Instituto de Hortofruticultura Subtropical y Mediterránea; España
description Octoploid genotypes of Asparagus officinalis L. cv. ‘Morado de Huetor’, a tetraploid Spanish landrace, were successfully induced by treating in vitro rhizome buds explants with colchicine. Pulses during 24 h with different concentrations of colchicine are able to induce polyploid plants, colchicine applied at 0.1 % induce a 7 % of octoploid genotypes with a stable ploidy level and a 3.5 % of mixoploid genotypes. The maximum rate of mixoploid genotypes (26 %) was obtained with 0.3 % colchicine. The plant survival and rooting rates of explants decrease for increasing doses of colchicine. All octoploid genotypes were micropropagated, rooted, transplanted and successfully acclimatized (100 %) in a glasshouse. The four octoploid plants recovered show significantly better agronomical parameters such as spear diameter, canopy area and shoot length than the original tetraploid plants and histological studies confirm the size increase of octoploid cells respect the original tetraploid cells.
publishDate 2014
dc.date.none.fl_str_mv 2014-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/42682
Carmona Martín, E.; Regalado González, Jose Javier; Raghavan, L.; Encina, C. L.; In vitro induction of autooctoploid asparagus genotypes; Springer; Plant Cell, Tissue and Organ Culture; 121; 1; 12-2014; 249-254
0167-6857
1573-5044
CONICET Digital
CONICET
url http://hdl.handle.net/11336/42682
identifier_str_mv Carmona Martín, E.; Regalado González, Jose Javier; Raghavan, L.; Encina, C. L.; In vitro induction of autooctoploid asparagus genotypes; Springer; Plant Cell, Tissue and Organ Culture; 121; 1; 12-2014; 249-254
0167-6857
1573-5044
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s11240-014-0680-4
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11240-014-0680-4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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