The fliR gene contributes to the virulence of S. marcescens in a Drosophila intestinal infection model

Autores
Sina Rahme, Bechara; Lestradet, Matthieu; Di Venanzio, Gisela Andrea; Ayyaz, Arshad; Wennida Yamba, Miriam; Lazzaro, Martina; Liégeois, Samuel; Garcia Vescovi, Eleonora; Ferrandon, Dominique
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Serratia marcescens is an opportunistic bacterium that infects a wide range of hosts including humans. It is a potent pathogen in a septic injury model of Drosophila melanogaster since a few bacteria directly injected in the body cavity kill the insect within a day. In contrast, flies do not succumb to ingested bacteria for days even though some bacteria cross the intestinal barrier into the hemolymph within hours. The mechanisms by which S. marcescens attacks enterocytes and damages the intestinal epithelium remain uncharacterized. To better understand intestinal infections, we performed a genetic screen for loss of virulence of ingested S. marcescens and identified FliR, a structural component of the flagellum, as a virulence factor. Next, we compared the virulence of two flagellum mutants fliR and flhD in two distinct S. marcescens strains. Both genes are required for S. marcescens to escape the gut lumen into the hemocoel, indicating that the flagellum plays an important role for the passage of bacteria through the intestinal barrier. Unexpectedly, fliR but not flhD is involved in S. marcescens-mediated damages of the intestinal epithelium that ultimately contribute to the demise of the host. Our results therefore suggest a flagellum-independent role for fliR in bacterial virulence.
Fil: Sina Rahme, Bechara. Centre National de la Recherche Scientifique; Francia
Fil: Lestradet, Matthieu. Centre National de la Recherche Scientifique; Francia
Fil: Di Venanzio, Gisela Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Ayyaz, Arshad. Centre National de la Recherche Scientifique; Francia
Fil: Wennida Yamba, Miriam. Centre National de la Recherche Scientifique; Francia
Fil: Lazzaro, Martina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Liégeois, Samuel. Centre National de la Recherche Scientifique; Francia
Fil: Garcia Vescovi, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Ferrandon, Dominique. Centre National de la Recherche Scientifique; Francia
Materia
Serratia
FliR
Drosophila
virulence
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/213723
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/213723
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The fliR gene contributes to the virulence of S. marcescens in a Drosophila intestinal infection modelSina Rahme, BecharaLestradet, MatthieuDi Venanzio, Gisela AndreaAyyaz, ArshadWennida Yamba, MiriamLazzaro, MartinaLiégeois, SamuelGarcia Vescovi, EleonoraFerrandon, DominiqueSerratiaFliRDrosophilavirulencehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Serratia marcescens is an opportunistic bacterium that infects a wide range of hosts including humans. It is a potent pathogen in a septic injury model of Drosophila melanogaster since a few bacteria directly injected in the body cavity kill the insect within a day. In contrast, flies do not succumb to ingested bacteria for days even though some bacteria cross the intestinal barrier into the hemolymph within hours. The mechanisms by which S. marcescens attacks enterocytes and damages the intestinal epithelium remain uncharacterized. To better understand intestinal infections, we performed a genetic screen for loss of virulence of ingested S. marcescens and identified FliR, a structural component of the flagellum, as a virulence factor. Next, we compared the virulence of two flagellum mutants fliR and flhD in two distinct S. marcescens strains. Both genes are required for S. marcescens to escape the gut lumen into the hemocoel, indicating that the flagellum plays an important role for the passage of bacteria through the intestinal barrier. Unexpectedly, fliR but not flhD is involved in S. marcescens-mediated damages of the intestinal epithelium that ultimately contribute to the demise of the host. Our results therefore suggest a flagellum-independent role for fliR in bacterial virulence.Fil: Sina Rahme, Bechara. Centre National de la Recherche Scientifique; FranciaFil: Lestradet, Matthieu. Centre National de la Recherche Scientifique; FranciaFil: Di Venanzio, Gisela Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Ayyaz, Arshad. Centre National de la Recherche Scientifique; FranciaFil: Wennida Yamba, Miriam. Centre National de la Recherche Scientifique; FranciaFil: Lazzaro, Martina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Liégeois, Samuel. Centre National de la Recherche Scientifique; FranciaFil: Garcia Vescovi, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Ferrandon, Dominique. Centre National de la Recherche Scientifique; FranciaNature2022-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/213723Sina Rahme, Bechara; Lestradet, Matthieu; Di Venanzio, Gisela Andrea; Ayyaz, Arshad; Wennida Yamba, Miriam; et al.; The fliR gene contributes to the virulence of S. marcescens in a Drosophila intestinal infection model; Nature; Scientific Reports; 12; 1; 2-2022; 1-102045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-022-06780-winfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:28Zoai:ri.conicet.gov.ar:11336/213723instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:28.586CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The fliR gene contributes to the virulence of S. marcescens in a Drosophila intestinal infection model
title The fliR gene contributes to the virulence of S. marcescens in a Drosophila intestinal infection model
spellingShingle The fliR gene contributes to the virulence of S. marcescens in a Drosophila intestinal infection model
Sina Rahme, Bechara
Serratia
FliR
Drosophila
virulence
title_short The fliR gene contributes to the virulence of S. marcescens in a Drosophila intestinal infection model
title_full The fliR gene contributes to the virulence of S. marcescens in a Drosophila intestinal infection model
title_fullStr The fliR gene contributes to the virulence of S. marcescens in a Drosophila intestinal infection model
title_full_unstemmed The fliR gene contributes to the virulence of S. marcescens in a Drosophila intestinal infection model
title_sort The fliR gene contributes to the virulence of S. marcescens in a Drosophila intestinal infection model
dc.creator.none.fl_str_mv Sina Rahme, Bechara
Lestradet, Matthieu
Di Venanzio, Gisela Andrea
Ayyaz, Arshad
Wennida Yamba, Miriam
Lazzaro, Martina
Liégeois, Samuel
Garcia Vescovi, Eleonora
Ferrandon, Dominique
author Sina Rahme, Bechara
author_facet Sina Rahme, Bechara
Lestradet, Matthieu
Di Venanzio, Gisela Andrea
Ayyaz, Arshad
Wennida Yamba, Miriam
Lazzaro, Martina
Liégeois, Samuel
Garcia Vescovi, Eleonora
Ferrandon, Dominique
author_role author
author2 Lestradet, Matthieu
Di Venanzio, Gisela Andrea
Ayyaz, Arshad
Wennida Yamba, Miriam
Lazzaro, Martina
Liégeois, Samuel
Garcia Vescovi, Eleonora
Ferrandon, Dominique
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Serratia
FliR
Drosophila
virulence
topic Serratia
FliR
Drosophila
virulence
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Serratia marcescens is an opportunistic bacterium that infects a wide range of hosts including humans. It is a potent pathogen in a septic injury model of Drosophila melanogaster since a few bacteria directly injected in the body cavity kill the insect within a day. In contrast, flies do not succumb to ingested bacteria for days even though some bacteria cross the intestinal barrier into the hemolymph within hours. The mechanisms by which S. marcescens attacks enterocytes and damages the intestinal epithelium remain uncharacterized. To better understand intestinal infections, we performed a genetic screen for loss of virulence of ingested S. marcescens and identified FliR, a structural component of the flagellum, as a virulence factor. Next, we compared the virulence of two flagellum mutants fliR and flhD in two distinct S. marcescens strains. Both genes are required for S. marcescens to escape the gut lumen into the hemocoel, indicating that the flagellum plays an important role for the passage of bacteria through the intestinal barrier. Unexpectedly, fliR but not flhD is involved in S. marcescens-mediated damages of the intestinal epithelium that ultimately contribute to the demise of the host. Our results therefore suggest a flagellum-independent role for fliR in bacterial virulence.
Fil: Sina Rahme, Bechara. Centre National de la Recherche Scientifique; Francia
Fil: Lestradet, Matthieu. Centre National de la Recherche Scientifique; Francia
Fil: Di Venanzio, Gisela Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Ayyaz, Arshad. Centre National de la Recherche Scientifique; Francia
Fil: Wennida Yamba, Miriam. Centre National de la Recherche Scientifique; Francia
Fil: Lazzaro, Martina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Liégeois, Samuel. Centre National de la Recherche Scientifique; Francia
Fil: Garcia Vescovi, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Ferrandon, Dominique. Centre National de la Recherche Scientifique; Francia
description Serratia marcescens is an opportunistic bacterium that infects a wide range of hosts including humans. It is a potent pathogen in a septic injury model of Drosophila melanogaster since a few bacteria directly injected in the body cavity kill the insect within a day. In contrast, flies do not succumb to ingested bacteria for days even though some bacteria cross the intestinal barrier into the hemolymph within hours. The mechanisms by which S. marcescens attacks enterocytes and damages the intestinal epithelium remain uncharacterized. To better understand intestinal infections, we performed a genetic screen for loss of virulence of ingested S. marcescens and identified FliR, a structural component of the flagellum, as a virulence factor. Next, we compared the virulence of two flagellum mutants fliR and flhD in two distinct S. marcescens strains. Both genes are required for S. marcescens to escape the gut lumen into the hemocoel, indicating that the flagellum plays an important role for the passage of bacteria through the intestinal barrier. Unexpectedly, fliR but not flhD is involved in S. marcescens-mediated damages of the intestinal epithelium that ultimately contribute to the demise of the host. Our results therefore suggest a flagellum-independent role for fliR in bacterial virulence.
publishDate 2022
dc.date.none.fl_str_mv 2022-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/213723
Sina Rahme, Bechara; Lestradet, Matthieu; Di Venanzio, Gisela Andrea; Ayyaz, Arshad; Wennida Yamba, Miriam; et al.; The fliR gene contributes to the virulence of S. marcescens in a Drosophila intestinal infection model; Nature; Scientific Reports; 12; 1; 2-2022; 1-10
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/213723
identifier_str_mv Sina Rahme, Bechara; Lestradet, Matthieu; Di Venanzio, Gisela Andrea; Ayyaz, Arshad; Wennida Yamba, Miriam; et al.; The fliR gene contributes to the virulence of S. marcescens in a Drosophila intestinal infection model; Nature; Scientific Reports; 12; 1; 2-2022; 1-10
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-022-06780-w
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature
publisher.none.fl_str_mv Nature
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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