Implementation of an epidemiological surveillance strategy for COVID-19 focused on groups at high risk of SARS-CoV-2 infection
- Autores
- Esandi, María del Carmen; Marinovich, Joel; Bergé, Ignacio; García, Nicolás Ariel; German, Olga Lorena; Ayala Peña, Victoria Belen; Sierra, Fermín; Serralunga, María Gabriela; Esandi, Maria Eugenia; del Valle, Marta; Bouzat, Cecilia Beatriz
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- The COVID-19 pandemic has been a challenge to implement strategies to mitigate the transmission of the SARS-CoV-2 virus. Asymptomatic transmission among healthcare workers (HCWs) at the front line of care is particularly concerning due to the potential emergence of outbreaks at healthcare centers. With the final goal of early identification and isolation of asymptomatic infected HCWs, we validated a pooled-sample screening and assessed implementation outcomes and results of an epidemiological surveillance strategy carried out during a 12-month period at public health institutions in Bahía Blanca. To develop and validate a coronavirus detection method, RNA was extracted from nasopharyngeal swabs and iden- tification of the viral E gene was done by an “in-house” RT-qPCR using Taqman probes and the human gene RNAseP as a control. Validation against a commercial kit demonstrated high sensibility and specificity of our test (95%, IC 95%: [85%-100%]). To increase our testing capacity, we validated sample pooling (n= 5) prior to RNA extraction. The results showed a sensibility of 73% (IC 95%: [46%- 99%]) and specificity of 100% against individuals. A tailor-made soft- ware called “VIGI-COVID” was designed to properly manage data. A prospective cohort study was conducted since 15/09/20 to 15/09/21. 860 HCWs were included in the epidemiological surveillance and 1765 swabs were performed. The annual cumulative incidence was 2,30% IC95% [1,26% - 3,39%] (20/860), and 43% of the 860 HCWs were swabbed more than once. Our study demonstrated the utili- ty of comprehensive screening of asymptomatic HCWs during the COVID19 pandemic. Early identification and isolation of infected HCWs prevent the onward transmission of SARS-CoV-2, reducing the risk of healthcare-associated outbreaks.
Fil: Esandi, María del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Marinovich, Joel. Hospital Municipal Doctor Leónidas Lucero; Argentina
Fil: Bergé, Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: García, Nicolás Ariel. Universidad Nacional del Sur. Departamento de Física; Argentina
Fil: German, Olga Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Ayala Peña, Victoria Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Sierra, Fermín. Hospital Municipal Doctor Leónidas Lucero; Argentina
Fil: Serralunga, María Gabriela. Universidad Nacional del Sur; Argentina
Fil: Esandi, Maria Eugenia. Universidad Nacional del Sur. Departamento de Economía; Argentina
Fil: del Valle, Marta. Hospital Municipal Doctor Leónidas Lucero; Argentina
Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Reunión anual de las Sociedades de Biociencia: LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión anual de la Sociedad Argentina de Immunología; LIII Reunión anual de la Asociación Argentina de Farmacología Experimental y XI Reunión anual de la Asociación Argentina de Nanomedicinas
Reunión Virtual
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Nanomedicinas
Asociación Argentina de Nanomedicinas - Materia
-
pandemia
screening
PCR
Sars-CoV2
COVID-19 - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/184785
Ver los metadatos del registro completo
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Implementation of an epidemiological surveillance strategy for COVID-19 focused on groups at high risk of SARS-CoV-2 infectionEsandi, María del CarmenMarinovich, JoelBergé, IgnacioGarcía, Nicolás ArielGerman, Olga LorenaAyala Peña, Victoria BelenSierra, FermínSerralunga, María GabrielaEsandi, Maria Eugeniadel Valle, MartaBouzat, Cecilia BeatrizpandemiascreeningPCRSars-CoV2COVID-19https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The COVID-19 pandemic has been a challenge to implement strategies to mitigate the transmission of the SARS-CoV-2 virus. Asymptomatic transmission among healthcare workers (HCWs) at the front line of care is particularly concerning due to the potential emergence of outbreaks at healthcare centers. With the final goal of early identification and isolation of asymptomatic infected HCWs, we validated a pooled-sample screening and assessed implementation outcomes and results of an epidemiological surveillance strategy carried out during a 12-month period at public health institutions in Bahía Blanca. To develop and validate a coronavirus detection method, RNA was extracted from nasopharyngeal swabs and iden- tification of the viral E gene was done by an “in-house” RT-qPCR using Taqman probes and the human gene RNAseP as a control. Validation against a commercial kit demonstrated high sensibility and specificity of our test (95%, IC 95%: [85%-100%]). To increase our testing capacity, we validated sample pooling (n= 5) prior to RNA extraction. The results showed a sensibility of 73% (IC 95%: [46%- 99%]) and specificity of 100% against individuals. A tailor-made soft- ware called “VIGI-COVID” was designed to properly manage data. A prospective cohort study was conducted since 15/09/20 to 15/09/21. 860 HCWs were included in the epidemiological surveillance and 1765 swabs were performed. The annual cumulative incidence was 2,30% IC95% [1,26% - 3,39%] (20/860), and 43% of the 860 HCWs were swabbed more than once. Our study demonstrated the utili- ty of comprehensive screening of asymptomatic HCWs during the COVID19 pandemic. Early identification and isolation of infected HCWs prevent the onward transmission of SARS-CoV-2, reducing the risk of healthcare-associated outbreaks.Fil: Esandi, María del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Marinovich, Joel. Hospital Municipal Doctor Leónidas Lucero; ArgentinaFil: Bergé, Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: García, Nicolás Ariel. Universidad Nacional del Sur. Departamento de Física; ArgentinaFil: German, Olga Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Ayala Peña, Victoria Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Sierra, Fermín. Hospital Municipal Doctor Leónidas Lucero; ArgentinaFil: Serralunga, María Gabriela. Universidad Nacional del Sur; ArgentinaFil: Esandi, Maria Eugenia. Universidad Nacional del Sur. Departamento de Economía; ArgentinaFil: del Valle, Marta. Hospital Municipal Doctor Leónidas Lucero; ArgentinaFil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaReunión anual de las Sociedades de Biociencia: LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión anual de la Sociedad Argentina de Immunología; LIII Reunión anual de la Asociación Argentina de Farmacología Experimental y XI Reunión anual de la Asociación Argentina de NanomedicinasReunión VirtualArgentinaSociedad Argentina de Investigación ClínicaAsociación Argentina de Farmacología ExperimentalAsociación Argentina de NanomedicinasAsociación Argentina de NanomedicinasFundación Revista Medicina2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/184785Implementation of an epidemiological surveillance strategy for COVID-19 focused on groups at high risk of SARS-CoV-2 infection; Reunión anual de las Sociedades de Biociencia: LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión anual de la Sociedad Argentina de Immunología; LIII Reunión anual de la Asociación Argentina de Farmacología Experimental y XI Reunión anual de la Asociación Argentina de Nanomedicinas; Reunión Virtual; Argentina; 2021; 117-1171669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/revista-medicinaNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:55:07Zoai:ri.conicet.gov.ar:11336/184785instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:55:07.449CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Implementation of an epidemiological surveillance strategy for COVID-19 focused on groups at high risk of SARS-CoV-2 infection |
| title |
Implementation of an epidemiological surveillance strategy for COVID-19 focused on groups at high risk of SARS-CoV-2 infection |
| spellingShingle |
Implementation of an epidemiological surveillance strategy for COVID-19 focused on groups at high risk of SARS-CoV-2 infection Esandi, María del Carmen pandemia screening PCR Sars-CoV2 COVID-19 |
| title_short |
Implementation of an epidemiological surveillance strategy for COVID-19 focused on groups at high risk of SARS-CoV-2 infection |
| title_full |
Implementation of an epidemiological surveillance strategy for COVID-19 focused on groups at high risk of SARS-CoV-2 infection |
| title_fullStr |
Implementation of an epidemiological surveillance strategy for COVID-19 focused on groups at high risk of SARS-CoV-2 infection |
| title_full_unstemmed |
Implementation of an epidemiological surveillance strategy for COVID-19 focused on groups at high risk of SARS-CoV-2 infection |
| title_sort |
Implementation of an epidemiological surveillance strategy for COVID-19 focused on groups at high risk of SARS-CoV-2 infection |
| dc.creator.none.fl_str_mv |
Esandi, María del Carmen Marinovich, Joel Bergé, Ignacio García, Nicolás Ariel German, Olga Lorena Ayala Peña, Victoria Belen Sierra, Fermín Serralunga, María Gabriela Esandi, Maria Eugenia del Valle, Marta Bouzat, Cecilia Beatriz |
| author |
Esandi, María del Carmen |
| author_facet |
Esandi, María del Carmen Marinovich, Joel Bergé, Ignacio García, Nicolás Ariel German, Olga Lorena Ayala Peña, Victoria Belen Sierra, Fermín Serralunga, María Gabriela Esandi, Maria Eugenia del Valle, Marta Bouzat, Cecilia Beatriz |
| author_role |
author |
| author2 |
Marinovich, Joel Bergé, Ignacio García, Nicolás Ariel German, Olga Lorena Ayala Peña, Victoria Belen Sierra, Fermín Serralunga, María Gabriela Esandi, Maria Eugenia del Valle, Marta Bouzat, Cecilia Beatriz |
| author2_role |
author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
pandemia screening PCR Sars-CoV2 COVID-19 |
| topic |
pandemia screening PCR Sars-CoV2 COVID-19 |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
The COVID-19 pandemic has been a challenge to implement strategies to mitigate the transmission of the SARS-CoV-2 virus. Asymptomatic transmission among healthcare workers (HCWs) at the front line of care is particularly concerning due to the potential emergence of outbreaks at healthcare centers. With the final goal of early identification and isolation of asymptomatic infected HCWs, we validated a pooled-sample screening and assessed implementation outcomes and results of an epidemiological surveillance strategy carried out during a 12-month period at public health institutions in Bahía Blanca. To develop and validate a coronavirus detection method, RNA was extracted from nasopharyngeal swabs and iden- tification of the viral E gene was done by an “in-house” RT-qPCR using Taqman probes and the human gene RNAseP as a control. Validation against a commercial kit demonstrated high sensibility and specificity of our test (95%, IC 95%: [85%-100%]). To increase our testing capacity, we validated sample pooling (n= 5) prior to RNA extraction. The results showed a sensibility of 73% (IC 95%: [46%- 99%]) and specificity of 100% against individuals. A tailor-made soft- ware called “VIGI-COVID” was designed to properly manage data. A prospective cohort study was conducted since 15/09/20 to 15/09/21. 860 HCWs were included in the epidemiological surveillance and 1765 swabs were performed. The annual cumulative incidence was 2,30% IC95% [1,26% - 3,39%] (20/860), and 43% of the 860 HCWs were swabbed more than once. Our study demonstrated the utili- ty of comprehensive screening of asymptomatic HCWs during the COVID19 pandemic. Early identification and isolation of infected HCWs prevent the onward transmission of SARS-CoV-2, reducing the risk of healthcare-associated outbreaks. Fil: Esandi, María del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina Fil: Marinovich, Joel. Hospital Municipal Doctor Leónidas Lucero; Argentina Fil: Bergé, Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina Fil: García, Nicolás Ariel. Universidad Nacional del Sur. Departamento de Física; Argentina Fil: German, Olga Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina Fil: Ayala Peña, Victoria Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina Fil: Sierra, Fermín. Hospital Municipal Doctor Leónidas Lucero; Argentina Fil: Serralunga, María Gabriela. Universidad Nacional del Sur; Argentina Fil: Esandi, Maria Eugenia. Universidad Nacional del Sur. Departamento de Economía; Argentina Fil: del Valle, Marta. Hospital Municipal Doctor Leónidas Lucero; Argentina Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina Reunión anual de las Sociedades de Biociencia: LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión anual de la Sociedad Argentina de Immunología; LIII Reunión anual de la Asociación Argentina de Farmacología Experimental y XI Reunión anual de la Asociación Argentina de Nanomedicinas Reunión Virtual Argentina Sociedad Argentina de Investigación Clínica Asociación Argentina de Farmacología Experimental Asociación Argentina de Nanomedicinas Asociación Argentina de Nanomedicinas |
| description |
The COVID-19 pandemic has been a challenge to implement strategies to mitigate the transmission of the SARS-CoV-2 virus. Asymptomatic transmission among healthcare workers (HCWs) at the front line of care is particularly concerning due to the potential emergence of outbreaks at healthcare centers. With the final goal of early identification and isolation of asymptomatic infected HCWs, we validated a pooled-sample screening and assessed implementation outcomes and results of an epidemiological surveillance strategy carried out during a 12-month period at public health institutions in Bahía Blanca. To develop and validate a coronavirus detection method, RNA was extracted from nasopharyngeal swabs and iden- tification of the viral E gene was done by an “in-house” RT-qPCR using Taqman probes and the human gene RNAseP as a control. Validation against a commercial kit demonstrated high sensibility and specificity of our test (95%, IC 95%: [85%-100%]). To increase our testing capacity, we validated sample pooling (n= 5) prior to RNA extraction. The results showed a sensibility of 73% (IC 95%: [46%- 99%]) and specificity of 100% against individuals. A tailor-made soft- ware called “VIGI-COVID” was designed to properly manage data. A prospective cohort study was conducted since 15/09/20 to 15/09/21. 860 HCWs were included in the epidemiological surveillance and 1765 swabs were performed. The annual cumulative incidence was 2,30% IC95% [1,26% - 3,39%] (20/860), and 43% of the 860 HCWs were swabbed more than once. Our study demonstrated the utili- ty of comprehensive screening of asymptomatic HCWs during the COVID19 pandemic. Early identification and isolation of infected HCWs prevent the onward transmission of SARS-CoV-2, reducing the risk of healthcare-associated outbreaks. |
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Implementation of an epidemiological surveillance strategy for COVID-19 focused on groups at high risk of SARS-CoV-2 infection; Reunión anual de las Sociedades de Biociencia: LXVI Reunión anual de la Sociedad Argentina de Investigación Clínica; LXIX Reunión anual de la Sociedad Argentina de Immunología; LIII Reunión anual de la Asociación Argentina de Farmacología Experimental y XI Reunión anual de la Asociación Argentina de Nanomedicinas; Reunión Virtual; Argentina; 2021; 117-117 1669-9106 CONICET Digital CONICET |
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