Carrier in carrier: DNA-doxorubicin complex self-assembled with amphiphilic cyclodextrins as nanosystems for cancer therapy

Autores
Alarcón, Liliana; Andrada, Heber Eduardo; Falcone, Ruben Dario; Silva, Fernando Oscar; Olivera, Maria Eugenia
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The goal of the present work was to characterize the interaction between DNA-Doxorubicin (DNA-Dox) complex and catanionic vesicles based on amphiphilic cyclodextrins (ModCBHD) for their application as drug delivery systems. The systems were characterized by dynamic light scattering (DLS), Zeta potential (ζ), circular dichroism (CD), Emission spectroscopy, atomic force microscope (AFM), transmission electron microscopy (TEM) and drug release in two-compartment Franz cells. Fluorescence spectra, CD profile and ζ showed multifaceted interaction pathways between DNA and Dox, with ionic and intercalation interactions, trought of the amino sugar residue and the tetracene ring system of the drug, to form DNA-Dox complexes with a negative surface charge. DNA behaves as a reservoir of Dox, that is slowly released from the complex triggered the presence of ions the medium. ModCBHD-DNA-Dox complexes were formed by self-assembling in aqueous solution without introducing any subsequent steps, and exhibited around 160 nm particle size, monodisperse size distribution (PDI 0.250) and spherical shape, which could be an advantage for the enhanced permeability and retention (EPR) effect in cancer therapy. All these results demonstrated that ModCBHD can load the DNA-Dox complex and indicated the potential of the ModCBHD-DNA-Dox systems as nanocarriers to be evaluated in both in vitro cancer cell lines and in vivo tumor models.
Fil: Alarcón, Liliana. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
Fil: Andrada, Heber Eduardo. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Falcone, Ruben Dario. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Silva, Fernando Oscar. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Olivera, Maria Eugenia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
6ta Reunión Internacional de Ciencias Farmacéuticas (RICiFa 2020+1)
Córdoba y Rosario
Argentina
Universidad Nacional de Córdoba
Universidad Nacional de Rosario
Materia
Amphiphilic Ciclodextrins
DNA-Doxorubicin complex
Drug delivery
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/232953
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/232953
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Carrier in carrier: DNA-doxorubicin complex self-assembled with amphiphilic cyclodextrins as nanosystems for cancer therapyAlarcón, LilianaAndrada, Heber EduardoFalcone, Ruben DarioSilva, Fernando OscarOlivera, Maria EugeniaAmphiphilic CiclodextrinsDNA-Doxorubicin complexDrug deliveryhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The goal of the present work was to characterize the interaction between DNA-Doxorubicin (DNA-Dox) complex and catanionic vesicles based on amphiphilic cyclodextrins (ModCBHD) for their application as drug delivery systems. The systems were characterized by dynamic light scattering (DLS), Zeta potential (ζ), circular dichroism (CD), Emission spectroscopy, atomic force microscope (AFM), transmission electron microscopy (TEM) and drug release in two-compartment Franz cells. Fluorescence spectra, CD profile and ζ showed multifaceted interaction pathways between DNA and Dox, with ionic and intercalation interactions, trought of the amino sugar residue and the tetracene ring system of the drug, to form DNA-Dox complexes with a negative surface charge. DNA behaves as a reservoir of Dox, that is slowly released from the complex triggered the presence of ions the medium. ModCBHD-DNA-Dox complexes were formed by self-assembling in aqueous solution without introducing any subsequent steps, and exhibited around 160 nm particle size, monodisperse size distribution (PDI 0.250) and spherical shape, which could be an advantage for the enhanced permeability and retention (EPR) effect in cancer therapy. All these results demonstrated that ModCBHD can load the DNA-Dox complex and indicated the potential of the ModCBHD-DNA-Dox systems as nanocarriers to be evaluated in both in vitro cancer cell lines and in vivo tumor models.Fil: Alarcón, Liliana. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; ArgentinaFil: Andrada, Heber Eduardo. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Falcone, Ruben Dario. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Silva, Fernando Oscar. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Olivera, Maria Eugenia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina6ta Reunión Internacional de Ciencias Farmacéuticas (RICiFa 2020+1)Córdoba y RosarioArgentinaUniversidad Nacional de CórdobaUniversidad Nacional de RosarioUniversidad Nacional de Córdoba2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/232953Carrier in carrier: DNA-doxorubicin complex self-assembled with amphiphilic cyclodextrins as nanosystems for cancer therapy; 6ta Reunión Internacional de Ciencias Farmacéuticas (RICiFa 2020+1); Córdoba y Rosario; Argentina; 2021; 1-2CONICET DigitalCONICETengInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:01:17Zoai:ri.conicet.gov.ar:11336/232953instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:01:18.014CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Carrier in carrier: DNA-doxorubicin complex self-assembled with amphiphilic cyclodextrins as nanosystems for cancer therapy
title Carrier in carrier: DNA-doxorubicin complex self-assembled with amphiphilic cyclodextrins as nanosystems for cancer therapy
spellingShingle Carrier in carrier: DNA-doxorubicin complex self-assembled with amphiphilic cyclodextrins as nanosystems for cancer therapy
Alarcón, Liliana
Amphiphilic Ciclodextrins
DNA-Doxorubicin complex
Drug delivery
title_short Carrier in carrier: DNA-doxorubicin complex self-assembled with amphiphilic cyclodextrins as nanosystems for cancer therapy
title_full Carrier in carrier: DNA-doxorubicin complex self-assembled with amphiphilic cyclodextrins as nanosystems for cancer therapy
title_fullStr Carrier in carrier: DNA-doxorubicin complex self-assembled with amphiphilic cyclodextrins as nanosystems for cancer therapy
title_full_unstemmed Carrier in carrier: DNA-doxorubicin complex self-assembled with amphiphilic cyclodextrins as nanosystems for cancer therapy
title_sort Carrier in carrier: DNA-doxorubicin complex self-assembled with amphiphilic cyclodextrins as nanosystems for cancer therapy
dc.creator.none.fl_str_mv Alarcón, Liliana
Andrada, Heber Eduardo
Falcone, Ruben Dario
Silva, Fernando Oscar
Olivera, Maria Eugenia
author Alarcón, Liliana
author_facet Alarcón, Liliana
Andrada, Heber Eduardo
Falcone, Ruben Dario
Silva, Fernando Oscar
Olivera, Maria Eugenia
author_role author
author2 Andrada, Heber Eduardo
Falcone, Ruben Dario
Silva, Fernando Oscar
Olivera, Maria Eugenia
author2_role author
author
author
author
dc.subject.none.fl_str_mv Amphiphilic Ciclodextrins
DNA-Doxorubicin complex
Drug delivery
topic Amphiphilic Ciclodextrins
DNA-Doxorubicin complex
Drug delivery
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The goal of the present work was to characterize the interaction between DNA-Doxorubicin (DNA-Dox) complex and catanionic vesicles based on amphiphilic cyclodextrins (ModCBHD) for their application as drug delivery systems. The systems were characterized by dynamic light scattering (DLS), Zeta potential (ζ), circular dichroism (CD), Emission spectroscopy, atomic force microscope (AFM), transmission electron microscopy (TEM) and drug release in two-compartment Franz cells. Fluorescence spectra, CD profile and ζ showed multifaceted interaction pathways between DNA and Dox, with ionic and intercalation interactions, trought of the amino sugar residue and the tetracene ring system of the drug, to form DNA-Dox complexes with a negative surface charge. DNA behaves as a reservoir of Dox, that is slowly released from the complex triggered the presence of ions the medium. ModCBHD-DNA-Dox complexes were formed by self-assembling in aqueous solution without introducing any subsequent steps, and exhibited around 160 nm particle size, monodisperse size distribution (PDI 0.250) and spherical shape, which could be an advantage for the enhanced permeability and retention (EPR) effect in cancer therapy. All these results demonstrated that ModCBHD can load the DNA-Dox complex and indicated the potential of the ModCBHD-DNA-Dox systems as nanocarriers to be evaluated in both in vitro cancer cell lines and in vivo tumor models.
Fil: Alarcón, Liliana. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
Fil: Andrada, Heber Eduardo. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Falcone, Ruben Dario. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Silva, Fernando Oscar. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Olivera, Maria Eugenia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
6ta Reunión Internacional de Ciencias Farmacéuticas (RICiFa 2020+1)
Córdoba y Rosario
Argentina
Universidad Nacional de Córdoba
Universidad Nacional de Rosario
description The goal of the present work was to characterize the interaction between DNA-Doxorubicin (DNA-Dox) complex and catanionic vesicles based on amphiphilic cyclodextrins (ModCBHD) for their application as drug delivery systems. The systems were characterized by dynamic light scattering (DLS), Zeta potential (ζ), circular dichroism (CD), Emission spectroscopy, atomic force microscope (AFM), transmission electron microscopy (TEM) and drug release in two-compartment Franz cells. Fluorescence spectra, CD profile and ζ showed multifaceted interaction pathways between DNA and Dox, with ionic and intercalation interactions, trought of the amino sugar residue and the tetracene ring system of the drug, to form DNA-Dox complexes with a negative surface charge. DNA behaves as a reservoir of Dox, that is slowly released from the complex triggered the presence of ions the medium. ModCBHD-DNA-Dox complexes were formed by self-assembling in aqueous solution without introducing any subsequent steps, and exhibited around 160 nm particle size, monodisperse size distribution (PDI 0.250) and spherical shape, which could be an advantage for the enhanced permeability and retention (EPR) effect in cancer therapy. All these results demonstrated that ModCBHD can load the DNA-Dox complex and indicated the potential of the ModCBHD-DNA-Dox systems as nanocarriers to be evaluated in both in vitro cancer cell lines and in vivo tumor models.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/232953
Carrier in carrier: DNA-doxorubicin complex self-assembled with amphiphilic cyclodextrins as nanosystems for cancer therapy; 6ta Reunión Internacional de Ciencias Farmacéuticas (RICiFa 2020+1); Córdoba y Rosario; Argentina; 2021; 1-2
CONICET Digital
CONICET
url http://hdl.handle.net/11336/232953
identifier_str_mv Carrier in carrier: DNA-doxorubicin complex self-assembled with amphiphilic cyclodextrins as nanosystems for cancer therapy; 6ta Reunión Internacional de Ciencias Farmacéuticas (RICiFa 2020+1); Córdoba y Rosario; Argentina; 2021; 1-2
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Universidad Nacional de Córdoba
publisher.none.fl_str_mv Universidad Nacional de Córdoba
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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