Binding and Encapsulation of Doxorubicin on Smart Pectin Hydrogels for Oral Delivery
- Autores
- Bosio, Valeria Elizabeth; Machain, Victoria; Gómez López, Azucena; Pérez de Berti, Ignacio Omar; Marchetti, Sergio Gustavo; Mechetti, Magdalena; Castro, Guillermo Raúl
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Pectins (Pec) of 33 to 74 % esterification degree were tested with doxorubicin (Dox), a very high toxic drug widely used in cancer therapies. Pec with 35 and 55 % DE were selected because of the Dox binding higher than Pec microspheres of 35 and 55 % obtained by ionotropic gelation with Ca+2 have 88 and 66 % Dox loading capacity. Kinetic Dox release showed more than 80.0 and about 30.0 % free drug from 35 % and 55 % Pec formulations at pH 7.4, and 37 °C after 1-h incubation, respectively. Besides, Dox release decrease to 12 % in 55 % Pec microsphere formulation after 1-year storage at 4 °C. FTIR analysis of Pec–Dox complex showed hipsochromic shifts for the σC0O, δN-H and σC-C vibrational modes compared to Dox spectrum suggesting strong interaction between the drug cargo and the matrix. Rheological studies of Pec and Pec–Dox samples flow behavior exhibited a shear-thinning nature. Fifty-five percent of Pec showed higher viscosity than the viscosity for 35 % Pec in all range of temperatures analyzed, and decreased when the temperature is raised. Besides, Pec–Dox complexes have higher viscosity values than those of the corresponding Pec samples, and viscosity curves as function of shear rate for 35 % Pec–Dox are above the curves of 55 % Pec–Dox. In both cases, the results are confirming significant interaction between the cargo and the matrix, which also was established in viscoelastic dynamic analysis.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Centro de Investigación y Desarrollo en Ciencias Aplicadas - Materia
-
Química
Drug delivery
Doxorubicin
Pectin
FTIR
Rheology - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
.jpg)
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/123865
Ver los metadatos del registro completo
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Binding and Encapsulation of Doxorubicin on Smart Pectin Hydrogels for Oral DeliveryBosio, Valeria ElizabethMachain, VictoriaGómez López, AzucenaPérez de Berti, Ignacio OmarMarchetti, Sergio GustavoMechetti, MagdalenaCastro, Guillermo RaúlQuímicaDrug deliveryDoxorubicinPectinFTIRRheologyPectins (Pec) of 33 to 74 % esterification degree were tested with doxorubicin (Dox), a very high toxic drug widely used in cancer therapies. Pec with 35 and 55 % DE were selected because of the Dox binding higher than Pec microspheres of 35 and 55 % obtained by ionotropic gelation with Ca+2 have 88 and 66 % Dox loading capacity. Kinetic Dox release showed more than 80.0 and about 30.0 % free drug from 35 % and 55 % Pec formulations at pH 7.4, and 37 °C after 1-h incubation, respectively. Besides, Dox release decrease to 12 % in 55 % Pec microsphere formulation after 1-year storage at 4 °C. FTIR analysis of Pec–Dox complex showed hipsochromic shifts for the σC0O, δN-H and σC-C vibrational modes compared to Dox spectrum suggesting strong interaction between the drug cargo and the matrix. Rheological studies of Pec and Pec–Dox samples flow behavior exhibited a shear-thinning nature. Fifty-five percent of Pec showed higher viscosity than the viscosity for 35 % Pec in all range of temperatures analyzed, and decreased when the temperature is raised. Besides, Pec–Dox complexes have higher viscosity values than those of the corresponding Pec samples, and viscosity curves as function of shear rate for 35 % Pec–Dox are above the curves of 55 % Pec–Dox. In both cases, the results are confirming significant interaction between the cargo and the matrix, which also was established in viscoelastic dynamic analysis.Centro de Investigación y Desarrollo en Fermentaciones IndustrialesCentro de Investigación y Desarrollo en Ciencias Aplicadas2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1365-1376http://sedici.unlp.edu.ar/handle/10915/123865enginfo:eu-repo/semantics/altIdentifier/issn/1559-0291info:eu-repo/semantics/altIdentifier/doi/10.1007/s12010-012-9641-8info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-22T17:10:34Zoai:sedici.unlp.edu.ar:10915/123865Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-22 17:10:35.254SEDICI (UNLP) - Universidad Nacional de La Platafalse |
| dc.title.none.fl_str_mv |
Binding and Encapsulation of Doxorubicin on Smart Pectin Hydrogels for Oral Delivery |
| title |
Binding and Encapsulation of Doxorubicin on Smart Pectin Hydrogels for Oral Delivery |
| spellingShingle |
Binding and Encapsulation of Doxorubicin on Smart Pectin Hydrogels for Oral Delivery Bosio, Valeria Elizabeth Química Drug delivery Doxorubicin Pectin FTIR Rheology |
| title_short |
Binding and Encapsulation of Doxorubicin on Smart Pectin Hydrogels for Oral Delivery |
| title_full |
Binding and Encapsulation of Doxorubicin on Smart Pectin Hydrogels for Oral Delivery |
| title_fullStr |
Binding and Encapsulation of Doxorubicin on Smart Pectin Hydrogels for Oral Delivery |
| title_full_unstemmed |
Binding and Encapsulation of Doxorubicin on Smart Pectin Hydrogels for Oral Delivery |
| title_sort |
Binding and Encapsulation of Doxorubicin on Smart Pectin Hydrogels for Oral Delivery |
| dc.creator.none.fl_str_mv |
Bosio, Valeria Elizabeth Machain, Victoria Gómez López, Azucena Pérez de Berti, Ignacio Omar Marchetti, Sergio Gustavo Mechetti, Magdalena Castro, Guillermo Raúl |
| author |
Bosio, Valeria Elizabeth |
| author_facet |
Bosio, Valeria Elizabeth Machain, Victoria Gómez López, Azucena Pérez de Berti, Ignacio Omar Marchetti, Sergio Gustavo Mechetti, Magdalena Castro, Guillermo Raúl |
| author_role |
author |
| author2 |
Machain, Victoria Gómez López, Azucena Pérez de Berti, Ignacio Omar Marchetti, Sergio Gustavo Mechetti, Magdalena Castro, Guillermo Raúl |
| author2_role |
author author author author author author |
| dc.subject.none.fl_str_mv |
Química Drug delivery Doxorubicin Pectin FTIR Rheology |
| topic |
Química Drug delivery Doxorubicin Pectin FTIR Rheology |
| dc.description.none.fl_txt_mv |
Pectins (Pec) of 33 to 74 % esterification degree were tested with doxorubicin (Dox), a very high toxic drug widely used in cancer therapies. Pec with 35 and 55 % DE were selected because of the Dox binding higher than Pec microspheres of 35 and 55 % obtained by ionotropic gelation with Ca+2 have 88 and 66 % Dox loading capacity. Kinetic Dox release showed more than 80.0 and about 30.0 % free drug from 35 % and 55 % Pec formulations at pH 7.4, and 37 °C after 1-h incubation, respectively. Besides, Dox release decrease to 12 % in 55 % Pec microsphere formulation after 1-year storage at 4 °C. FTIR analysis of Pec–Dox complex showed hipsochromic shifts for the σC0O, δN-H and σC-C vibrational modes compared to Dox spectrum suggesting strong interaction between the drug cargo and the matrix. Rheological studies of Pec and Pec–Dox samples flow behavior exhibited a shear-thinning nature. Fifty-five percent of Pec showed higher viscosity than the viscosity for 35 % Pec in all range of temperatures analyzed, and decreased when the temperature is raised. Besides, Pec–Dox complexes have higher viscosity values than those of the corresponding Pec samples, and viscosity curves as function of shear rate for 35 % Pec–Dox are above the curves of 55 % Pec–Dox. In both cases, the results are confirming significant interaction between the cargo and the matrix, which also was established in viscoelastic dynamic analysis. Centro de Investigación y Desarrollo en Fermentaciones Industriales Centro de Investigación y Desarrollo en Ciencias Aplicadas |
| description |
Pectins (Pec) of 33 to 74 % esterification degree were tested with doxorubicin (Dox), a very high toxic drug widely used in cancer therapies. Pec with 35 and 55 % DE were selected because of the Dox binding higher than Pec microspheres of 35 and 55 % obtained by ionotropic gelation with Ca+2 have 88 and 66 % Dox loading capacity. Kinetic Dox release showed more than 80.0 and about 30.0 % free drug from 35 % and 55 % Pec formulations at pH 7.4, and 37 °C after 1-h incubation, respectively. Besides, Dox release decrease to 12 % in 55 % Pec microsphere formulation after 1-year storage at 4 °C. FTIR analysis of Pec–Dox complex showed hipsochromic shifts for the σC0O, δN-H and σC-C vibrational modes compared to Dox spectrum suggesting strong interaction between the drug cargo and the matrix. Rheological studies of Pec and Pec–Dox samples flow behavior exhibited a shear-thinning nature. Fifty-five percent of Pec showed higher viscosity than the viscosity for 35 % Pec in all range of temperatures analyzed, and decreased when the temperature is raised. Besides, Pec–Dox complexes have higher viscosity values than those of the corresponding Pec samples, and viscosity curves as function of shear rate for 35 % Pec–Dox are above the curves of 55 % Pec–Dox. In both cases, the results are confirming significant interaction between the cargo and the matrix, which also was established in viscoelastic dynamic analysis. |
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2012 |
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2012 |
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