Interaction of Helja Lectib isolatd from Helianthus Annus with Candida Albicans inhibits Phagocytosis and promotes phenotypic maturation in dendritic cells

Autores
Chop, Maia; Radicioni, Melisa Belén; del Rio, Marianela Victoria; Rodriguez Rodrigues, Christian; Regente, Mariana Clelia
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Background: Helja is a sunflower lectin that recognizes mannose motifs. Candidiasis is a fungal infection caused by Candida spp. that affects immunocompromised individuals. This opportunistic yeast can be recognized by dendritic cells (DCs). The aim of this work is to analyze if the interaction between Helja and Candida albicans (Ca) cell wall could inhibit recognition and phagocytosis by DCs and modulate cell maturation. Methods: Bone marrow dendritic cells (BMDCs) were cultured in RPMI 1640 medium supplemented with FLT3-L. Helja was purified from sunflower seeds with a D-mannose-agarose resin. Phagocytosis assay was evaluated by FACS and Confocal Microscopy. C. albicans (NGY152 strain) were stained with FITC and then incubated with Helja for 45 minutes prior to BMDC stimulation. LPS (100 ng/ml) and 4°C incubation were used as controls. BMDCs phenotype was analyzed by FACS. Results: Confocal images and quantification of labeled-yeast revealed that the preincubation of fungal cells with Helja inhibited recognition by BMDCs and induced a statistically significant reduction on phagocytosis compared to control (n=2, ****p<0.0001 Helja-Ca+LPS-treated BMDCs vs Ca+LPS-treated BMDCs). This observation was confirmed by measuring FITC+ dots in the BMDC gate by FACS analysis (Helja-Ca treated BMDCs -53,7%- vs Ca-treated BMDCs -60.4%). Finally, Ca preincubated with Helja improved the upregulation of MHCII compared to untreated cells or Ca-stimulated BMDCs (n=3 **p<0.01). The same pattern was observed in the co-stimulatory molecule CD86, Helja-Ca treated BMDCs induced a significant increase in the percentage of CD86+ cells (54.1% vs 42.0% observed in Ca-treated BMDCS or 24.0% observed in untreated cells, n=3). Conclusions: These data suggest that the mannose-binding lectin blocks epitopes on Candida cell wall important for DC recognition. However, Helja promotes DCs maturation, a process required to activate adaptive immunity, highlighting its potential biomedical application.
Fil: Chop, Maia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Radicioni, Melisa Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: del Rio, Marianela Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Rodriguez Rodrigues, Christian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Regente, Mariana Clelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología & 3er Congreso Franco Argentino de Inmunología y Reunión Anual 2022 de la Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Materia
MANNOSE BINDING LECTIN
CANDIDA ALBICANS
DENDRITIC CELLS
CELL MODULATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/262180
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/262180
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Interaction of Helja Lectib isolatd from Helianthus Annus with Candida Albicans inhibits Phagocytosis and promotes phenotypic maturation in dendritic cellsChop, MaiaRadicioni, Melisa Beléndel Rio, Marianela VictoriaRodriguez Rodrigues, ChristianRegente, Mariana CleliaMANNOSE BINDING LECTINCANDIDA ALBICANSDENDRITIC CELLSCELL MODULATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Helja is a sunflower lectin that recognizes mannose motifs. Candidiasis is a fungal infection caused by Candida spp. that affects immunocompromised individuals. This opportunistic yeast can be recognized by dendritic cells (DCs). The aim of this work is to analyze if the interaction between Helja and Candida albicans (Ca) cell wall could inhibit recognition and phagocytosis by DCs and modulate cell maturation. Methods: Bone marrow dendritic cells (BMDCs) were cultured in RPMI 1640 medium supplemented with FLT3-L. Helja was purified from sunflower seeds with a D-mannose-agarose resin. Phagocytosis assay was evaluated by FACS and Confocal Microscopy. C. albicans (NGY152 strain) were stained with FITC and then incubated with Helja for 45 minutes prior to BMDC stimulation. LPS (100 ng/ml) and 4°C incubation were used as controls. BMDCs phenotype was analyzed by FACS. Results: Confocal images and quantification of labeled-yeast revealed that the preincubation of fungal cells with Helja inhibited recognition by BMDCs and induced a statistically significant reduction on phagocytosis compared to control (n=2, ****p<0.0001 Helja-Ca+LPS-treated BMDCs vs Ca+LPS-treated BMDCs). This observation was confirmed by measuring FITC+ dots in the BMDC gate by FACS analysis (Helja-Ca treated BMDCs -53,7%- vs Ca-treated BMDCs -60.4%). Finally, Ca preincubated with Helja improved the upregulation of MHCII compared to untreated cells or Ca-stimulated BMDCs (n=3 **p<0.01). The same pattern was observed in the co-stimulatory molecule CD86, Helja-Ca treated BMDCs induced a significant increase in the percentage of CD86+ cells (54.1% vs 42.0% observed in Ca-treated BMDCS or 24.0% observed in untreated cells, n=3). Conclusions: These data suggest that the mannose-binding lectin blocks epitopes on Candida cell wall important for DC recognition. However, Helja promotes DCs maturation, a process required to activate adaptive immunity, highlighting its potential biomedical application.Fil: Chop, Maia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaFil: Radicioni, Melisa Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: del Rio, Marianela Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Rodriguez Rodrigues, Christian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaFil: Regente, Mariana Clelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaLXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología & 3er Congreso Franco Argentino de Inmunología y Reunión Anual 2022 de la Sociedad Argentina de FisiologíaMar del PlataArgentinaSociedad Argentina de Investigación ClínicaFundación Revista Medicina2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/262180Interaction of Helja Lectib isolatd from Helianthus Annus with Candida Albicans inhibits Phagocytosis and promotes phenotypic maturation in dendritic cells; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología & 3er Congreso Franco Argentino de Inmunología y Reunión Anual 2022 de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2022; 149-1491669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/revistas/vol82-22/s5/1s5.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:20:40Zoai:ri.conicet.gov.ar:11336/262180instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:20:41.138CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Interaction of Helja Lectib isolatd from Helianthus Annus with Candida Albicans inhibits Phagocytosis and promotes phenotypic maturation in dendritic cells
title Interaction of Helja Lectib isolatd from Helianthus Annus with Candida Albicans inhibits Phagocytosis and promotes phenotypic maturation in dendritic cells
spellingShingle Interaction of Helja Lectib isolatd from Helianthus Annus with Candida Albicans inhibits Phagocytosis and promotes phenotypic maturation in dendritic cells
Chop, Maia
MANNOSE BINDING LECTIN
CANDIDA ALBICANS
DENDRITIC CELLS
CELL MODULATION
title_short Interaction of Helja Lectib isolatd from Helianthus Annus with Candida Albicans inhibits Phagocytosis and promotes phenotypic maturation in dendritic cells
title_full Interaction of Helja Lectib isolatd from Helianthus Annus with Candida Albicans inhibits Phagocytosis and promotes phenotypic maturation in dendritic cells
title_fullStr Interaction of Helja Lectib isolatd from Helianthus Annus with Candida Albicans inhibits Phagocytosis and promotes phenotypic maturation in dendritic cells
title_full_unstemmed Interaction of Helja Lectib isolatd from Helianthus Annus with Candida Albicans inhibits Phagocytosis and promotes phenotypic maturation in dendritic cells
title_sort Interaction of Helja Lectib isolatd from Helianthus Annus with Candida Albicans inhibits Phagocytosis and promotes phenotypic maturation in dendritic cells
dc.creator.none.fl_str_mv Chop, Maia
Radicioni, Melisa Belén
del Rio, Marianela Victoria
Rodriguez Rodrigues, Christian
Regente, Mariana Clelia
author Chop, Maia
author_facet Chop, Maia
Radicioni, Melisa Belén
del Rio, Marianela Victoria
Rodriguez Rodrigues, Christian
Regente, Mariana Clelia
author_role author
author2 Radicioni, Melisa Belén
del Rio, Marianela Victoria
Rodriguez Rodrigues, Christian
Regente, Mariana Clelia
author2_role author
author
author
author
dc.subject.none.fl_str_mv MANNOSE BINDING LECTIN
CANDIDA ALBICANS
DENDRITIC CELLS
CELL MODULATION
topic MANNOSE BINDING LECTIN
CANDIDA ALBICANS
DENDRITIC CELLS
CELL MODULATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: Helja is a sunflower lectin that recognizes mannose motifs. Candidiasis is a fungal infection caused by Candida spp. that affects immunocompromised individuals. This opportunistic yeast can be recognized by dendritic cells (DCs). The aim of this work is to analyze if the interaction between Helja and Candida albicans (Ca) cell wall could inhibit recognition and phagocytosis by DCs and modulate cell maturation. Methods: Bone marrow dendritic cells (BMDCs) were cultured in RPMI 1640 medium supplemented with FLT3-L. Helja was purified from sunflower seeds with a D-mannose-agarose resin. Phagocytosis assay was evaluated by FACS and Confocal Microscopy. C. albicans (NGY152 strain) were stained with FITC and then incubated with Helja for 45 minutes prior to BMDC stimulation. LPS (100 ng/ml) and 4°C incubation were used as controls. BMDCs phenotype was analyzed by FACS. Results: Confocal images and quantification of labeled-yeast revealed that the preincubation of fungal cells with Helja inhibited recognition by BMDCs and induced a statistically significant reduction on phagocytosis compared to control (n=2, ****p<0.0001 Helja-Ca+LPS-treated BMDCs vs Ca+LPS-treated BMDCs). This observation was confirmed by measuring FITC+ dots in the BMDC gate by FACS analysis (Helja-Ca treated BMDCs -53,7%- vs Ca-treated BMDCs -60.4%). Finally, Ca preincubated with Helja improved the upregulation of MHCII compared to untreated cells or Ca-stimulated BMDCs (n=3 **p<0.01). The same pattern was observed in the co-stimulatory molecule CD86, Helja-Ca treated BMDCs induced a significant increase in the percentage of CD86+ cells (54.1% vs 42.0% observed in Ca-treated BMDCS or 24.0% observed in untreated cells, n=3). Conclusions: These data suggest that the mannose-binding lectin blocks epitopes on Candida cell wall important for DC recognition. However, Helja promotes DCs maturation, a process required to activate adaptive immunity, highlighting its potential biomedical application.
Fil: Chop, Maia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Radicioni, Melisa Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: del Rio, Marianela Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Rodriguez Rodrigues, Christian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Regente, Mariana Clelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología & 3er Congreso Franco Argentino de Inmunología y Reunión Anual 2022 de la Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
description Background: Helja is a sunflower lectin that recognizes mannose motifs. Candidiasis is a fungal infection caused by Candida spp. that affects immunocompromised individuals. This opportunistic yeast can be recognized by dendritic cells (DCs). The aim of this work is to analyze if the interaction between Helja and Candida albicans (Ca) cell wall could inhibit recognition and phagocytosis by DCs and modulate cell maturation. Methods: Bone marrow dendritic cells (BMDCs) were cultured in RPMI 1640 medium supplemented with FLT3-L. Helja was purified from sunflower seeds with a D-mannose-agarose resin. Phagocytosis assay was evaluated by FACS and Confocal Microscopy. C. albicans (NGY152 strain) were stained with FITC and then incubated with Helja for 45 minutes prior to BMDC stimulation. LPS (100 ng/ml) and 4°C incubation were used as controls. BMDCs phenotype was analyzed by FACS. Results: Confocal images and quantification of labeled-yeast revealed that the preincubation of fungal cells with Helja inhibited recognition by BMDCs and induced a statistically significant reduction on phagocytosis compared to control (n=2, ****p<0.0001 Helja-Ca+LPS-treated BMDCs vs Ca+LPS-treated BMDCs). This observation was confirmed by measuring FITC+ dots in the BMDC gate by FACS analysis (Helja-Ca treated BMDCs -53,7%- vs Ca-treated BMDCs -60.4%). Finally, Ca preincubated with Helja improved the upregulation of MHCII compared to untreated cells or Ca-stimulated BMDCs (n=3 **p<0.01). The same pattern was observed in the co-stimulatory molecule CD86, Helja-Ca treated BMDCs induced a significant increase in the percentage of CD86+ cells (54.1% vs 42.0% observed in Ca-treated BMDCS or 24.0% observed in untreated cells, n=3). Conclusions: These data suggest that the mannose-binding lectin blocks epitopes on Candida cell wall important for DC recognition. However, Helja promotes DCs maturation, a process required to activate adaptive immunity, highlighting its potential biomedical application.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/262180
Interaction of Helja Lectib isolatd from Helianthus Annus with Candida Albicans inhibits Phagocytosis and promotes phenotypic maturation in dendritic cells; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología & 3er Congreso Franco Argentino de Inmunología y Reunión Anual 2022 de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2022; 149-149
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/262180
identifier_str_mv Interaction of Helja Lectib isolatd from Helianthus Annus with Candida Albicans inhibits Phagocytosis and promotes phenotypic maturation in dendritic cells; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología & 3er Congreso Franco Argentino de Inmunología y Reunión Anual 2022 de la Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2022; 149-149
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.medicinabuenosaires.com/revistas/vol82-22/s5/1s5.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
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dc.format.none.fl_str_mv application/pdf
application/pdf
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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