Unconventional protein secretion of Helja, an apoptotic lectin

Autores
Fassolari, Marisol; Pinedo, Marcela Lilian; de la Canal, Laura
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Proteins following unconventional protein secretion (UPS) bypass the RE-Golgi system to reach theapoplast. They use vesicular and no vesicular routes for their transport. Vesicular UPS can encompassdifferent pathways not fully characterized. In these work we analyze the route of Helja secretion, asunflower lectin lacking signal peptide but immunodetected extracellularly. Transient expression ofrecombinant Green fluorescent Helja (GFP-Helja) in Nicotiana Benthamiana leaves allow its apoplasticobservation verified by partial co-localization (Pearson r Value= 0.65) with the specific marker sec-RFP.Florescent intracellular punctate and mobile structures were also visualized. They did not co-localizewith the Golgi marker Sialyltransferase -RFP and their distribution was not affected by Brefeldin A, theinhibitor of RE-Golgi transport.Additionally, GFP-Helja neither co-localize with the multivesicular bodies marker Rha-RFP nor with theendocytic marker FM4-64. Our results support the UPS secretion of HELJA to the apoplast by a yetunidentified vesicular pathway.
Fil: Fassolari, Marisol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Pinedo, Marcela Lilian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: de la Canal, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
International Conference Plant Physiology & Biochemistry
Vienna
Austria
Vienna International Science Conferences & Events Association
Materia
Unconventional Protein secretion
sunflower lectin
vesicles
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/134752
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/134752
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Unconventional protein secretion of Helja, an apoptotic lectinFassolari, MarisolPinedo, Marcela Liliande la Canal, LauraUnconventional Protein secretionsunflower lectinvesicleshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Proteins following unconventional protein secretion (UPS) bypass the RE-Golgi system to reach theapoplast. They use vesicular and no vesicular routes for their transport. Vesicular UPS can encompassdifferent pathways not fully characterized. In these work we analyze the route of Helja secretion, asunflower lectin lacking signal peptide but immunodetected extracellularly. Transient expression ofrecombinant Green fluorescent Helja (GFP-Helja) in Nicotiana Benthamiana leaves allow its apoplasticobservation verified by partial co-localization (Pearson r Value= 0.65) with the specific marker sec-RFP.Florescent intracellular punctate and mobile structures were also visualized. They did not co-localizewith the Golgi marker Sialyltransferase -RFP and their distribution was not affected by Brefeldin A, theinhibitor of RE-Golgi transport.Additionally, GFP-Helja neither co-localize with the multivesicular bodies marker Rha-RFP nor with theendocytic marker FM4-64. Our results support the UPS secretion of HELJA to the apoplast by a yetunidentified vesicular pathway.Fil: Fassolari, Marisol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Pinedo, Marcela Lilian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: de la Canal, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaInternational Conference Plant Physiology & BiochemistryViennaAustriaVienna International Science Conferences & Events AssociationVienna International Science Conferences & Events Association2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectConferenciaBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/134752Unconventional protein secretion of Helja, an apoptotic lectin; International Conference Plant Physiology & Biochemistry; Vienna; Austria; 2018; 30-30CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://viscea.org/conferences/2018/Abstract_Books/Abstract_Book_PPB.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:09:48Zoai:ri.conicet.gov.ar:11336/134752instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:09:48.539CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Unconventional protein secretion of Helja, an apoptotic lectin
title Unconventional protein secretion of Helja, an apoptotic lectin
spellingShingle Unconventional protein secretion of Helja, an apoptotic lectin
Fassolari, Marisol
Unconventional Protein secretion
sunflower lectin
vesicles
title_short Unconventional protein secretion of Helja, an apoptotic lectin
title_full Unconventional protein secretion of Helja, an apoptotic lectin
title_fullStr Unconventional protein secretion of Helja, an apoptotic lectin
title_full_unstemmed Unconventional protein secretion of Helja, an apoptotic lectin
title_sort Unconventional protein secretion of Helja, an apoptotic lectin
dc.creator.none.fl_str_mv Fassolari, Marisol
Pinedo, Marcela Lilian
de la Canal, Laura
author Fassolari, Marisol
author_facet Fassolari, Marisol
Pinedo, Marcela Lilian
de la Canal, Laura
author_role author
author2 Pinedo, Marcela Lilian
de la Canal, Laura
author2_role author
author
dc.subject.none.fl_str_mv Unconventional Protein secretion
sunflower lectin
vesicles
topic Unconventional Protein secretion
sunflower lectin
vesicles
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Proteins following unconventional protein secretion (UPS) bypass the RE-Golgi system to reach theapoplast. They use vesicular and no vesicular routes for their transport. Vesicular UPS can encompassdifferent pathways not fully characterized. In these work we analyze the route of Helja secretion, asunflower lectin lacking signal peptide but immunodetected extracellularly. Transient expression ofrecombinant Green fluorescent Helja (GFP-Helja) in Nicotiana Benthamiana leaves allow its apoplasticobservation verified by partial co-localization (Pearson r Value= 0.65) with the specific marker sec-RFP.Florescent intracellular punctate and mobile structures were also visualized. They did not co-localizewith the Golgi marker Sialyltransferase -RFP and their distribution was not affected by Brefeldin A, theinhibitor of RE-Golgi transport.Additionally, GFP-Helja neither co-localize with the multivesicular bodies marker Rha-RFP nor with theendocytic marker FM4-64. Our results support the UPS secretion of HELJA to the apoplast by a yetunidentified vesicular pathway.
Fil: Fassolari, Marisol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Pinedo, Marcela Lilian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: de la Canal, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
International Conference Plant Physiology & Biochemistry
Vienna
Austria
Vienna International Science Conferences & Events Association
description Proteins following unconventional protein secretion (UPS) bypass the RE-Golgi system to reach theapoplast. They use vesicular and no vesicular routes for their transport. Vesicular UPS can encompassdifferent pathways not fully characterized. In these work we analyze the route of Helja secretion, asunflower lectin lacking signal peptide but immunodetected extracellularly. Transient expression ofrecombinant Green fluorescent Helja (GFP-Helja) in Nicotiana Benthamiana leaves allow its apoplasticobservation verified by partial co-localization (Pearson r Value= 0.65) with the specific marker sec-RFP.Florescent intracellular punctate and mobile structures were also visualized. They did not co-localizewith the Golgi marker Sialyltransferase -RFP and their distribution was not affected by Brefeldin A, theinhibitor of RE-Golgi transport.Additionally, GFP-Helja neither co-localize with the multivesicular bodies marker Rha-RFP nor with theendocytic marker FM4-64. Our results support the UPS secretion of HELJA to the apoplast by a yetunidentified vesicular pathway.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Conferencia
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/134752
Unconventional protein secretion of Helja, an apoptotic lectin; International Conference Plant Physiology & Biochemistry; Vienna; Austria; 2018; 30-30
CONICET Digital
CONICET
url http://hdl.handle.net/11336/134752
identifier_str_mv Unconventional protein secretion of Helja, an apoptotic lectin; International Conference Plant Physiology & Biochemistry; Vienna; Austria; 2018; 30-30
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://viscea.org/conferences/2018/Abstract_Books/Abstract_Book_PPB.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Vienna International Science Conferences & Events Association
publisher.none.fl_str_mv Vienna International Science Conferences & Events Association
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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