A lectin from Heliantus Annus recongizes GP120 from HIV-1 and promotes an inflammatory phenotype in dendritic cells
- Autores
- Chop, Maia; Radicioni, Melisa Belén; del Rio, Marianela Victoria; Sabatte, Juan Atilio; Regente, Mariana Clelia; Rodríguez Rodrígues, Christian Fernando Ariel
- Año de publicación
- 2023
- Idioma
- español castellano
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Background: Helja is a mannose-binding lectin that is isolated fromsunflower seeds. HIV-1 envelope glycoprotein (gp120) containshigh-mannose and complex N-glycans. The aim of this study wasto analyze whether Helja recognizes gp120 and induces maturationand pro-inflammatory cytokine transcription in dendritic cells (BMDCs).Methods: Helja was purified from Helianthus annuus seedsusing D-mannose-agarose affinity chromatography. Molecular interactionsbetween Helja and recombinant gp120 were evaluatedby dot and ligand blot approaches using anti-Helja antibodies. BMDCswere cultured in complete RPMI medium supplemented withFLT3-L. Cell phenotype was evaluated in Helja-treated BMDCs bymeasuring membrane proteins (CD86, CD40, MHCII, CD11c, CD80,Clec9a, Ly6G, SIPRα, CD103 and SiglecH) by FACS. BMDCs cytokineprofiles were analyzed using RT-qPCR (il-6, tnf-α, il-12, inf-αtgf-b, and il-10). Results: Helja-gp120 interactions were measuredby immunodetection and confirmed by ligand blot assays. A signaldot was observed at a MW of 95 kDa. Next, BMDCs were stimulatedwith Helja (10 μg/ml) to evaluate cell activation. The lectin induced amarked upregulation of MHCII, MHCI, CD80, CD40 and CD86 (n=3,**p<0.01 Helja-treated BMDCs vs control, *p<0.05 Helja+LPS-treatedBMDCs vs LPS control). No changes in Clec9a, Ly6G, SIRPα,CD103 and SiglecH were detected. Finally, Helja also induced thetranscriptional activity of pro-inflammatory and antiviral cytokines(il-6, tnf-α, il-12 and inf-α (n=3, *p<0,05, **p<0,01, ***p<0,001 vscontrol) resulting in an additive effect with LPS. No increase in anti-inflammatory cytokines (tgf-b, il-10) was observed. Conclusions:Helja recognizes specific glycosidic residues and arrangements ofgp120 in HIV-1, which induces dendritic cell maturation. This promotesimmune activation, leading to the release of proinflammatorycytokines by BMDCs. These results suggest that this lectin has potentialbiomedical applications as an antiviral agent.
Fil: Chop, Maia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Radicioni, Melisa Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: del Rio, Marianela Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Sabatte, Juan Atilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
Fil: Regente, Mariana Clelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Rodríguez Rodrígues, Christian Fernando Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Reunión de Sociedades de Biociencias 2023; LXVIII Annual Meeting of Sociedad Argentina de Investigación Clínica (Saic); Xxv Annual Conferences of Sociedad Argentina de Biología (Sab); Lv Annual Meeting of Asociación Argentina de Farmacología Experimental (Aafe); Viii Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (Aacytal)
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Biología
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio - Materia
-
MANNOSE-BINDING LECTIN
JACALIN
HIV
ANTIVIRAL - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/262011
Ver los metadatos del registro completo
id |
CONICETDig_79b85b009d10fb368489ad0e345d9dfc |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/262011 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
A lectin from Heliantus Annus recongizes GP120 from HIV-1 and promotes an inflammatory phenotype in dendritic cellsChop, MaiaRadicioni, Melisa Beléndel Rio, Marianela VictoriaSabatte, Juan AtilioRegente, Mariana CleliaRodríguez Rodrígues, Christian Fernando ArielMANNOSE-BINDING LECTINJACALINHIVANTIVIRALhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Helja is a mannose-binding lectin that is isolated fromsunflower seeds. HIV-1 envelope glycoprotein (gp120) containshigh-mannose and complex N-glycans. The aim of this study wasto analyze whether Helja recognizes gp120 and induces maturationand pro-inflammatory cytokine transcription in dendritic cells (BMDCs).Methods: Helja was purified from Helianthus annuus seedsusing D-mannose-agarose affinity chromatography. Molecular interactionsbetween Helja and recombinant gp120 were evaluatedby dot and ligand blot approaches using anti-Helja antibodies. BMDCswere cultured in complete RPMI medium supplemented withFLT3-L. Cell phenotype was evaluated in Helja-treated BMDCs bymeasuring membrane proteins (CD86, CD40, MHCII, CD11c, CD80,Clec9a, Ly6G, SIPRα, CD103 and SiglecH) by FACS. BMDCs cytokineprofiles were analyzed using RT-qPCR (il-6, tnf-α, il-12, inf-αtgf-b, and il-10). Results: Helja-gp120 interactions were measuredby immunodetection and confirmed by ligand blot assays. A signaldot was observed at a MW of 95 kDa. Next, BMDCs were stimulatedwith Helja (10 μg/ml) to evaluate cell activation. The lectin induced amarked upregulation of MHCII, MHCI, CD80, CD40 and CD86 (n=3,**p<0.01 Helja-treated BMDCs vs control, *p<0.05 Helja+LPS-treatedBMDCs vs LPS control). No changes in Clec9a, Ly6G, SIRPα,CD103 and SiglecH were detected. Finally, Helja also induced thetranscriptional activity of pro-inflammatory and antiviral cytokines(il-6, tnf-α, il-12 and inf-α (n=3, *p<0,05, **p<0,01, ***p<0,001 vscontrol) resulting in an additive effect with LPS. No increase in anti-inflammatory cytokines (tgf-b, il-10) was observed. Conclusions:Helja recognizes specific glycosidic residues and arrangements ofgp120 in HIV-1, which induces dendritic cell maturation. This promotesimmune activation, leading to the release of proinflammatorycytokines by BMDCs. These results suggest that this lectin has potentialbiomedical applications as an antiviral agent.Fil: Chop, Maia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaFil: Radicioni, Melisa Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: del Rio, Marianela Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Sabatte, Juan Atilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; ArgentinaFil: Regente, Mariana Clelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Rodríguez Rodrígues, Christian Fernando Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaReunión de Sociedades de Biociencias 2023; LXVIII Annual Meeting of Sociedad Argentina de Investigación Clínica (Saic); Xxv Annual Conferences of Sociedad Argentina de Biología (Sab); Lv Annual Meeting of Asociación Argentina de Farmacología Experimental (Aafe); Viii Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (Aacytal)Mar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de BiologíaAsociación Argentina de Farmacología ExperimentalAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioFundación Revista Medicina2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/262011A lectin from Heliantus Annus recongizes GP120 from HIV-1 and promotes an inflammatory phenotype in dendritic cells; Reunión de Sociedades de Biociencias 2023; LXVIII Annual Meeting of Sociedad Argentina de Investigación Clínica (Saic); Xxv Annual Conferences of Sociedad Argentina de Biología (Sab); Lv Annual Meeting of Asociación Argentina de Farmacología Experimental (Aafe); Viii Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (Aacytal); Mar del Plata; Argentina; 2023; 207-2081669-9106CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/revista-medicinaNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:43:05Zoai:ri.conicet.gov.ar:11336/262011instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:43:05.641CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
A lectin from Heliantus Annus recongizes GP120 from HIV-1 and promotes an inflammatory phenotype in dendritic cells |
title |
A lectin from Heliantus Annus recongizes GP120 from HIV-1 and promotes an inflammatory phenotype in dendritic cells |
spellingShingle |
A lectin from Heliantus Annus recongizes GP120 from HIV-1 and promotes an inflammatory phenotype in dendritic cells Chop, Maia MANNOSE-BINDING LECTIN JACALIN HIV ANTIVIRAL |
title_short |
A lectin from Heliantus Annus recongizes GP120 from HIV-1 and promotes an inflammatory phenotype in dendritic cells |
title_full |
A lectin from Heliantus Annus recongizes GP120 from HIV-1 and promotes an inflammatory phenotype in dendritic cells |
title_fullStr |
A lectin from Heliantus Annus recongizes GP120 from HIV-1 and promotes an inflammatory phenotype in dendritic cells |
title_full_unstemmed |
A lectin from Heliantus Annus recongizes GP120 from HIV-1 and promotes an inflammatory phenotype in dendritic cells |
title_sort |
A lectin from Heliantus Annus recongizes GP120 from HIV-1 and promotes an inflammatory phenotype in dendritic cells |
dc.creator.none.fl_str_mv |
Chop, Maia Radicioni, Melisa Belén del Rio, Marianela Victoria Sabatte, Juan Atilio Regente, Mariana Clelia Rodríguez Rodrígues, Christian Fernando Ariel |
author |
Chop, Maia |
author_facet |
Chop, Maia Radicioni, Melisa Belén del Rio, Marianela Victoria Sabatte, Juan Atilio Regente, Mariana Clelia Rodríguez Rodrígues, Christian Fernando Ariel |
author_role |
author |
author2 |
Radicioni, Melisa Belén del Rio, Marianela Victoria Sabatte, Juan Atilio Regente, Mariana Clelia Rodríguez Rodrígues, Christian Fernando Ariel |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
MANNOSE-BINDING LECTIN JACALIN HIV ANTIVIRAL |
topic |
MANNOSE-BINDING LECTIN JACALIN HIV ANTIVIRAL |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Background: Helja is a mannose-binding lectin that is isolated fromsunflower seeds. HIV-1 envelope glycoprotein (gp120) containshigh-mannose and complex N-glycans. The aim of this study wasto analyze whether Helja recognizes gp120 and induces maturationand pro-inflammatory cytokine transcription in dendritic cells (BMDCs).Methods: Helja was purified from Helianthus annuus seedsusing D-mannose-agarose affinity chromatography. Molecular interactionsbetween Helja and recombinant gp120 were evaluatedby dot and ligand blot approaches using anti-Helja antibodies. BMDCswere cultured in complete RPMI medium supplemented withFLT3-L. Cell phenotype was evaluated in Helja-treated BMDCs bymeasuring membrane proteins (CD86, CD40, MHCII, CD11c, CD80,Clec9a, Ly6G, SIPRα, CD103 and SiglecH) by FACS. BMDCs cytokineprofiles were analyzed using RT-qPCR (il-6, tnf-α, il-12, inf-αtgf-b, and il-10). Results: Helja-gp120 interactions were measuredby immunodetection and confirmed by ligand blot assays. A signaldot was observed at a MW of 95 kDa. Next, BMDCs were stimulatedwith Helja (10 μg/ml) to evaluate cell activation. The lectin induced amarked upregulation of MHCII, MHCI, CD80, CD40 and CD86 (n=3,**p<0.01 Helja-treated BMDCs vs control, *p<0.05 Helja+LPS-treatedBMDCs vs LPS control). No changes in Clec9a, Ly6G, SIRPα,CD103 and SiglecH were detected. Finally, Helja also induced thetranscriptional activity of pro-inflammatory and antiviral cytokines(il-6, tnf-α, il-12 and inf-α (n=3, *p<0,05, **p<0,01, ***p<0,001 vscontrol) resulting in an additive effect with LPS. No increase in anti-inflammatory cytokines (tgf-b, il-10) was observed. Conclusions:Helja recognizes specific glycosidic residues and arrangements ofgp120 in HIV-1, which induces dendritic cell maturation. This promotesimmune activation, leading to the release of proinflammatorycytokines by BMDCs. These results suggest that this lectin has potentialbiomedical applications as an antiviral agent. Fil: Chop, Maia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina Fil: Radicioni, Melisa Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina Fil: del Rio, Marianela Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina Fil: Sabatte, Juan Atilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Regente, Mariana Clelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina Fil: Rodríguez Rodrígues, Christian Fernando Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina Reunión de Sociedades de Biociencias 2023; LXVIII Annual Meeting of Sociedad Argentina de Investigación Clínica (Saic); Xxv Annual Conferences of Sociedad Argentina de Biología (Sab); Lv Annual Meeting of Asociación Argentina de Farmacología Experimental (Aafe); Viii Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (Aacytal) Mar del Plata Argentina Sociedad Argentina de Investigación Clínica Sociedad Argentina de Biología Asociación Argentina de Farmacología Experimental Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio |
description |
Background: Helja is a mannose-binding lectin that is isolated fromsunflower seeds. HIV-1 envelope glycoprotein (gp120) containshigh-mannose and complex N-glycans. The aim of this study wasto analyze whether Helja recognizes gp120 and induces maturationand pro-inflammatory cytokine transcription in dendritic cells (BMDCs).Methods: Helja was purified from Helianthus annuus seedsusing D-mannose-agarose affinity chromatography. Molecular interactionsbetween Helja and recombinant gp120 were evaluatedby dot and ligand blot approaches using anti-Helja antibodies. BMDCswere cultured in complete RPMI medium supplemented withFLT3-L. Cell phenotype was evaluated in Helja-treated BMDCs bymeasuring membrane proteins (CD86, CD40, MHCII, CD11c, CD80,Clec9a, Ly6G, SIPRα, CD103 and SiglecH) by FACS. BMDCs cytokineprofiles were analyzed using RT-qPCR (il-6, tnf-α, il-12, inf-αtgf-b, and il-10). Results: Helja-gp120 interactions were measuredby immunodetection and confirmed by ligand blot assays. A signaldot was observed at a MW of 95 kDa. Next, BMDCs were stimulatedwith Helja (10 μg/ml) to evaluate cell activation. The lectin induced amarked upregulation of MHCII, MHCI, CD80, CD40 and CD86 (n=3,**p<0.01 Helja-treated BMDCs vs control, *p<0.05 Helja+LPS-treatedBMDCs vs LPS control). No changes in Clec9a, Ly6G, SIRPα,CD103 and SiglecH were detected. Finally, Helja also induced thetranscriptional activity of pro-inflammatory and antiviral cytokines(il-6, tnf-α, il-12 and inf-α (n=3, *p<0,05, **p<0,01, ***p<0,001 vscontrol) resulting in an additive effect with LPS. No increase in anti-inflammatory cytokines (tgf-b, il-10) was observed. Conclusions:Helja recognizes specific glycosidic residues and arrangements ofgp120 in HIV-1, which induces dendritic cell maturation. This promotesimmune activation, leading to the release of proinflammatorycytokines by BMDCs. These results suggest that this lectin has potentialbiomedical applications as an antiviral agent. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Congreso Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/262011 A lectin from Heliantus Annus recongizes GP120 from HIV-1 and promotes an inflammatory phenotype in dendritic cells; Reunión de Sociedades de Biociencias 2023; LXVIII Annual Meeting of Sociedad Argentina de Investigación Clínica (Saic); Xxv Annual Conferences of Sociedad Argentina de Biología (Sab); Lv Annual Meeting of Asociación Argentina de Farmacología Experimental (Aafe); Viii Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (Aacytal); Mar del Plata; Argentina; 2023; 207-208 1669-9106 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/262011 |
identifier_str_mv |
A lectin from Heliantus Annus recongizes GP120 from HIV-1 and promotes an inflammatory phenotype in dendritic cells; Reunión de Sociedades de Biociencias 2023; LXVIII Annual Meeting of Sociedad Argentina de Investigación Clínica (Saic); Xxv Annual Conferences of Sociedad Argentina de Biología (Sab); Lv Annual Meeting of Asociación Argentina de Farmacología Experimental (Aafe); Viii Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (Aacytal); Mar del Plata; Argentina; 2023; 207-208 1669-9106 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
spa |
language |
spa |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/revista-medicina |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.coverage.none.fl_str_mv |
Nacional |
dc.publisher.none.fl_str_mv |
Fundación Revista Medicina |
publisher.none.fl_str_mv |
Fundación Revista Medicina |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844613355671650304 |
score |
13.070432 |