A lectin from Heliantus Annus recongizes GP120 from HIV-1 and promotes an inflammatory phenotype in dendritic cells

Autores
Chop, Maia; Radicioni, Melisa Belén; del Rio, Marianela Victoria; Sabatte, Juan Atilio; Regente, Mariana Clelia; Rodríguez Rodrígues, Christian Fernando Ariel
Año de publicación
2023
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Background: Helja is a mannose-binding lectin that is isolated fromsunflower seeds. HIV-1 envelope glycoprotein (gp120) containshigh-mannose and complex N-glycans. The aim of this study wasto analyze whether Helja recognizes gp120 and induces maturationand pro-inflammatory cytokine transcription in dendritic cells (BMDCs).Methods: Helja was purified from Helianthus annuus seedsusing D-mannose-agarose affinity chromatography. Molecular interactionsbetween Helja and recombinant gp120 were evaluatedby dot and ligand blot approaches using anti-Helja antibodies. BMDCswere cultured in complete RPMI medium supplemented withFLT3-L. Cell phenotype was evaluated in Helja-treated BMDCs bymeasuring membrane proteins (CD86, CD40, MHCII, CD11c, CD80,Clec9a, Ly6G, SIPRα, CD103 and SiglecH) by FACS. BMDCs cytokineprofiles were analyzed using RT-qPCR (il-6, tnf-α, il-12, inf-αtgf-b, and il-10). Results: Helja-gp120 interactions were measuredby immunodetection and confirmed by ligand blot assays. A signaldot was observed at a MW of 95 kDa. Next, BMDCs were stimulatedwith Helja (10 μg/ml) to evaluate cell activation. The lectin induced amarked upregulation of MHCII, MHCI, CD80, CD40 and CD86 (n=3,**p<0.01 Helja-treated BMDCs vs control, *p<0.05 Helja+LPS-treatedBMDCs vs LPS control). No changes in Clec9a, Ly6G, SIRPα,CD103 and SiglecH were detected. Finally, Helja also induced thetranscriptional activity of pro-inflammatory and antiviral cytokines(il-6, tnf-α, il-12 and inf-α (n=3, *p<0,05, **p<0,01, ***p<0,001 vscontrol) resulting in an additive effect with LPS. No increase in anti-inflammatory cytokines (tgf-b, il-10) was observed. Conclusions:Helja recognizes specific glycosidic residues and arrangements ofgp120 in HIV-1, which induces dendritic cell maturation. This promotesimmune activation, leading to the release of proinflammatorycytokines by BMDCs. These results suggest that this lectin has potentialbiomedical applications as an antiviral agent.
Fil: Chop, Maia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Radicioni, Melisa Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: del Rio, Marianela Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Sabatte, Juan Atilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
Fil: Regente, Mariana Clelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Rodríguez Rodrígues, Christian Fernando Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Reunión de Sociedades de Biociencias 2023; LXVIII Annual Meeting of Sociedad Argentina de Investigación Clínica (Saic); Xxv Annual Conferences of Sociedad Argentina de Biología (Sab); Lv Annual Meeting of Asociación Argentina de Farmacología Experimental (Aafe); Viii Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (Aacytal)
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Biología
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Materia
MANNOSE-BINDING LECTIN
JACALIN
HIV
ANTIVIRAL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/262011

id CONICETDig_79b85b009d10fb368489ad0e345d9dfc
oai_identifier_str oai:ri.conicet.gov.ar:11336/262011
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A lectin from Heliantus Annus recongizes GP120 from HIV-1 and promotes an inflammatory phenotype in dendritic cellsChop, MaiaRadicioni, Melisa Beléndel Rio, Marianela VictoriaSabatte, Juan AtilioRegente, Mariana CleliaRodríguez Rodrígues, Christian Fernando ArielMANNOSE-BINDING LECTINJACALINHIVANTIVIRALhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Helja is a mannose-binding lectin that is isolated fromsunflower seeds. HIV-1 envelope glycoprotein (gp120) containshigh-mannose and complex N-glycans. The aim of this study wasto analyze whether Helja recognizes gp120 and induces maturationand pro-inflammatory cytokine transcription in dendritic cells (BMDCs).Methods: Helja was purified from Helianthus annuus seedsusing D-mannose-agarose affinity chromatography. Molecular interactionsbetween Helja and recombinant gp120 were evaluatedby dot and ligand blot approaches using anti-Helja antibodies. BMDCswere cultured in complete RPMI medium supplemented withFLT3-L. Cell phenotype was evaluated in Helja-treated BMDCs bymeasuring membrane proteins (CD86, CD40, MHCII, CD11c, CD80,Clec9a, Ly6G, SIPRα, CD103 and SiglecH) by FACS. BMDCs cytokineprofiles were analyzed using RT-qPCR (il-6, tnf-α, il-12, inf-αtgf-b, and il-10). Results: Helja-gp120 interactions were measuredby immunodetection and confirmed by ligand blot assays. A signaldot was observed at a MW of 95 kDa. Next, BMDCs were stimulatedwith Helja (10 μg/ml) to evaluate cell activation. The lectin induced amarked upregulation of MHCII, MHCI, CD80, CD40 and CD86 (n=3,**p<0.01 Helja-treated BMDCs vs control, *p<0.05 Helja+LPS-treatedBMDCs vs LPS control). No changes in Clec9a, Ly6G, SIRPα,CD103 and SiglecH were detected. Finally, Helja also induced thetranscriptional activity of pro-inflammatory and antiviral cytokines(il-6, tnf-α, il-12 and inf-α (n=3, *p<0,05, **p<0,01, ***p<0,001 vscontrol) resulting in an additive effect with LPS. No increase in anti-inflammatory cytokines (tgf-b, il-10) was observed. Conclusions:Helja recognizes specific glycosidic residues and arrangements ofgp120 in HIV-1, which induces dendritic cell maturation. This promotesimmune activation, leading to the release of proinflammatorycytokines by BMDCs. These results suggest that this lectin has potentialbiomedical applications as an antiviral agent.Fil: Chop, Maia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaFil: Radicioni, Melisa Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: del Rio, Marianela Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Sabatte, Juan Atilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; ArgentinaFil: Regente, Mariana Clelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Rodríguez Rodrígues, Christian Fernando Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaReunión de Sociedades de Biociencias 2023; LXVIII Annual Meeting of Sociedad Argentina de Investigación Clínica (Saic); Xxv Annual Conferences of Sociedad Argentina de Biología (Sab); Lv Annual Meeting of Asociación Argentina de Farmacología Experimental (Aafe); Viii Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (Aacytal)Mar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de BiologíaAsociación Argentina de Farmacología ExperimentalAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioFundación Revista Medicina2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/262011A lectin from Heliantus Annus recongizes GP120 from HIV-1 and promotes an inflammatory phenotype in dendritic cells; Reunión de Sociedades de Biociencias 2023; LXVIII Annual Meeting of Sociedad Argentina de Investigación Clínica (Saic); Xxv Annual Conferences of Sociedad Argentina de Biología (Sab); Lv Annual Meeting of Asociación Argentina de Farmacología Experimental (Aafe); Viii Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (Aacytal); Mar del Plata; Argentina; 2023; 207-2081669-9106CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/revista-medicinaNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:43:05Zoai:ri.conicet.gov.ar:11336/262011instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:43:05.641CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A lectin from Heliantus Annus recongizes GP120 from HIV-1 and promotes an inflammatory phenotype in dendritic cells
title A lectin from Heliantus Annus recongizes GP120 from HIV-1 and promotes an inflammatory phenotype in dendritic cells
spellingShingle A lectin from Heliantus Annus recongizes GP120 from HIV-1 and promotes an inflammatory phenotype in dendritic cells
Chop, Maia
MANNOSE-BINDING LECTIN
JACALIN
HIV
ANTIVIRAL
title_short A lectin from Heliantus Annus recongizes GP120 from HIV-1 and promotes an inflammatory phenotype in dendritic cells
title_full A lectin from Heliantus Annus recongizes GP120 from HIV-1 and promotes an inflammatory phenotype in dendritic cells
title_fullStr A lectin from Heliantus Annus recongizes GP120 from HIV-1 and promotes an inflammatory phenotype in dendritic cells
title_full_unstemmed A lectin from Heliantus Annus recongizes GP120 from HIV-1 and promotes an inflammatory phenotype in dendritic cells
title_sort A lectin from Heliantus Annus recongizes GP120 from HIV-1 and promotes an inflammatory phenotype in dendritic cells
dc.creator.none.fl_str_mv Chop, Maia
Radicioni, Melisa Belén
del Rio, Marianela Victoria
Sabatte, Juan Atilio
Regente, Mariana Clelia
Rodríguez Rodrígues, Christian Fernando Ariel
author Chop, Maia
author_facet Chop, Maia
Radicioni, Melisa Belén
del Rio, Marianela Victoria
Sabatte, Juan Atilio
Regente, Mariana Clelia
Rodríguez Rodrígues, Christian Fernando Ariel
author_role author
author2 Radicioni, Melisa Belén
del Rio, Marianela Victoria
Sabatte, Juan Atilio
Regente, Mariana Clelia
Rodríguez Rodrígues, Christian Fernando Ariel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv MANNOSE-BINDING LECTIN
JACALIN
HIV
ANTIVIRAL
topic MANNOSE-BINDING LECTIN
JACALIN
HIV
ANTIVIRAL
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: Helja is a mannose-binding lectin that is isolated fromsunflower seeds. HIV-1 envelope glycoprotein (gp120) containshigh-mannose and complex N-glycans. The aim of this study wasto analyze whether Helja recognizes gp120 and induces maturationand pro-inflammatory cytokine transcription in dendritic cells (BMDCs).Methods: Helja was purified from Helianthus annuus seedsusing D-mannose-agarose affinity chromatography. Molecular interactionsbetween Helja and recombinant gp120 were evaluatedby dot and ligand blot approaches using anti-Helja antibodies. BMDCswere cultured in complete RPMI medium supplemented withFLT3-L. Cell phenotype was evaluated in Helja-treated BMDCs bymeasuring membrane proteins (CD86, CD40, MHCII, CD11c, CD80,Clec9a, Ly6G, SIPRα, CD103 and SiglecH) by FACS. BMDCs cytokineprofiles were analyzed using RT-qPCR (il-6, tnf-α, il-12, inf-αtgf-b, and il-10). Results: Helja-gp120 interactions were measuredby immunodetection and confirmed by ligand blot assays. A signaldot was observed at a MW of 95 kDa. Next, BMDCs were stimulatedwith Helja (10 μg/ml) to evaluate cell activation. The lectin induced amarked upregulation of MHCII, MHCI, CD80, CD40 and CD86 (n=3,**p<0.01 Helja-treated BMDCs vs control, *p<0.05 Helja+LPS-treatedBMDCs vs LPS control). No changes in Clec9a, Ly6G, SIRPα,CD103 and SiglecH were detected. Finally, Helja also induced thetranscriptional activity of pro-inflammatory and antiviral cytokines(il-6, tnf-α, il-12 and inf-α (n=3, *p<0,05, **p<0,01, ***p<0,001 vscontrol) resulting in an additive effect with LPS. No increase in anti-inflammatory cytokines (tgf-b, il-10) was observed. Conclusions:Helja recognizes specific glycosidic residues and arrangements ofgp120 in HIV-1, which induces dendritic cell maturation. This promotesimmune activation, leading to the release of proinflammatorycytokines by BMDCs. These results suggest that this lectin has potentialbiomedical applications as an antiviral agent.
Fil: Chop, Maia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Radicioni, Melisa Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: del Rio, Marianela Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Sabatte, Juan Atilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
Fil: Regente, Mariana Clelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Rodríguez Rodrígues, Christian Fernando Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Reunión de Sociedades de Biociencias 2023; LXVIII Annual Meeting of Sociedad Argentina de Investigación Clínica (Saic); Xxv Annual Conferences of Sociedad Argentina de Biología (Sab); Lv Annual Meeting of Asociación Argentina de Farmacología Experimental (Aafe); Viii Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (Aacytal)
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Biología
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
description Background: Helja is a mannose-binding lectin that is isolated fromsunflower seeds. HIV-1 envelope glycoprotein (gp120) containshigh-mannose and complex N-glycans. The aim of this study wasto analyze whether Helja recognizes gp120 and induces maturationand pro-inflammatory cytokine transcription in dendritic cells (BMDCs).Methods: Helja was purified from Helianthus annuus seedsusing D-mannose-agarose affinity chromatography. Molecular interactionsbetween Helja and recombinant gp120 were evaluatedby dot and ligand blot approaches using anti-Helja antibodies. BMDCswere cultured in complete RPMI medium supplemented withFLT3-L. Cell phenotype was evaluated in Helja-treated BMDCs bymeasuring membrane proteins (CD86, CD40, MHCII, CD11c, CD80,Clec9a, Ly6G, SIPRα, CD103 and SiglecH) by FACS. BMDCs cytokineprofiles were analyzed using RT-qPCR (il-6, tnf-α, il-12, inf-αtgf-b, and il-10). Results: Helja-gp120 interactions were measuredby immunodetection and confirmed by ligand blot assays. A signaldot was observed at a MW of 95 kDa. Next, BMDCs were stimulatedwith Helja (10 μg/ml) to evaluate cell activation. The lectin induced amarked upregulation of MHCII, MHCI, CD80, CD40 and CD86 (n=3,**p<0.01 Helja-treated BMDCs vs control, *p<0.05 Helja+LPS-treatedBMDCs vs LPS control). No changes in Clec9a, Ly6G, SIRPα,CD103 and SiglecH were detected. Finally, Helja also induced thetranscriptional activity of pro-inflammatory and antiviral cytokines(il-6, tnf-α, il-12 and inf-α (n=3, *p<0,05, **p<0,01, ***p<0,001 vscontrol) resulting in an additive effect with LPS. No increase in anti-inflammatory cytokines (tgf-b, il-10) was observed. Conclusions:Helja recognizes specific glycosidic residues and arrangements ofgp120 in HIV-1, which induces dendritic cell maturation. This promotesimmune activation, leading to the release of proinflammatorycytokines by BMDCs. These results suggest that this lectin has potentialbiomedical applications as an antiviral agent.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
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info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
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dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/262011
A lectin from Heliantus Annus recongizes GP120 from HIV-1 and promotes an inflammatory phenotype in dendritic cells; Reunión de Sociedades de Biociencias 2023; LXVIII Annual Meeting of Sociedad Argentina de Investigación Clínica (Saic); Xxv Annual Conferences of Sociedad Argentina de Biología (Sab); Lv Annual Meeting of Asociación Argentina de Farmacología Experimental (Aafe); Viii Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (Aacytal); Mar del Plata; Argentina; 2023; 207-208
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/262011
identifier_str_mv A lectin from Heliantus Annus recongizes GP120 from HIV-1 and promotes an inflammatory phenotype in dendritic cells; Reunión de Sociedades de Biociencias 2023; LXVIII Annual Meeting of Sociedad Argentina de Investigación Clínica (Saic); Xxv Annual Conferences of Sociedad Argentina de Biología (Sab); Lv Annual Meeting of Asociación Argentina de Farmacología Experimental (Aafe); Viii Regional Scientific Meeting of Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio (Aacytal); Mar del Plata; Argentina; 2023; 207-208
1669-9106
CONICET Digital
CONICET
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