A sustainable affinity partitioning process to recover papain from Carica papaya latex using alginate as macro-ligand

Autores
Rocha, María Victoria; Di Giacomo, Melisa; Beltramino, Sofia; Loh, Watson; Romanini, Diana; Nerli, Bibiana Beatriz
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The role of the natural biodegradable polymer alginate (ALG) as affinity macro-ligand for papain (PAP) was evaluated in order to design a new sustainable two-phase affinity strategy for recovering this enzyme from Carica papaya latex. In presence of PAP, decreased values of intrinsic viscosity and hydrodynamic radius of ALG were observed. These results suggested a neutralizing effect of PAP on the polymer negative charges which caused a decrease of intra-chain repulsion forces in ALG molecules and a consequent shrinkage of the polymer size. Calorimetric measurements demonstrated a cooperative interaction between PAP and ALG which was enthalpy-entropically driven. When partitioned C. papaya latex into aqueous two-phase systems (ATPSs) formed by polyethylene glycol (PEG) of MW 8000 and the biodegradable salt sodium citrate pH 5.20, only 20% of PAP was recovered at the PEG-enriched phase with a purification factor (PF) of 2.48. The addition of ALG 0.1% (w/w) into the system doubled the PAP partition coefficient (Kp, PAP), showing the ability of this polymer to enhance the enzyme recovery at the PEG-enriched phase and therefore, increasing the extraction efficiency. The subsequent addition of calcium chloride at a final concentration of 80 mM allowed the precipitation of the target enzyme and the recovering of PEG phase for recycling. The overall process showed a PAP recovery of 72% and a PF of 2.41. The proposed strategy not only conserved all the advantages of the reported extractions with PEG/ammonium sulfate (or phosphate) ATPSs, i.e., low cost, scalability and integration of the clarification and extraction steps, but also allowed overcoming their main drawbacks, i.e., the separation of the target molecule from the phase-polymer and the environmental impact caused by the disposal of these salts.
Fil: Rocha, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Di Giacomo, Melisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Beltramino, Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Loh, Watson. State university of Campinas; Brasil
Fil: Romanini, Diana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Nerli, Bibiana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Materia
Affinity Partitioning
Alginate
Aqueous Two-Phase Systems
Papain
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/52653
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/52653
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A sustainable affinity partitioning process to recover papain from Carica papaya latex using alginate as macro-ligandRocha, María VictoriaDi Giacomo, MelisaBeltramino, SofiaLoh, WatsonRomanini, DianaNerli, Bibiana BeatrizAffinity PartitioningAlginateAqueous Two-Phase SystemsPapainhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The role of the natural biodegradable polymer alginate (ALG) as affinity macro-ligand for papain (PAP) was evaluated in order to design a new sustainable two-phase affinity strategy for recovering this enzyme from Carica papaya latex. In presence of PAP, decreased values of intrinsic viscosity and hydrodynamic radius of ALG were observed. These results suggested a neutralizing effect of PAP on the polymer negative charges which caused a decrease of intra-chain repulsion forces in ALG molecules and a consequent shrinkage of the polymer size. Calorimetric measurements demonstrated a cooperative interaction between PAP and ALG which was enthalpy-entropically driven. When partitioned C. papaya latex into aqueous two-phase systems (ATPSs) formed by polyethylene glycol (PEG) of MW 8000 and the biodegradable salt sodium citrate pH 5.20, only 20% of PAP was recovered at the PEG-enriched phase with a purification factor (PF) of 2.48. The addition of ALG 0.1% (w/w) into the system doubled the PAP partition coefficient (Kp, PAP), showing the ability of this polymer to enhance the enzyme recovery at the PEG-enriched phase and therefore, increasing the extraction efficiency. The subsequent addition of calcium chloride at a final concentration of 80 mM allowed the precipitation of the target enzyme and the recovering of PEG phase for recycling. The overall process showed a PAP recovery of 72% and a PF of 2.41. The proposed strategy not only conserved all the advantages of the reported extractions with PEG/ammonium sulfate (or phosphate) ATPSs, i.e., low cost, scalability and integration of the clarification and extraction steps, but also allowed overcoming their main drawbacks, i.e., the separation of the target molecule from the phase-polymer and the environmental impact caused by the disposal of these salts.Fil: Rocha, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; ArgentinaFil: Di Giacomo, Melisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; ArgentinaFil: Beltramino, Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; ArgentinaFil: Loh, Watson. State university of Campinas; BrasilFil: Romanini, Diana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; ArgentinaFil: Nerli, Bibiana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; ArgentinaElsevier Science2016-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/52653Rocha, María Victoria; Di Giacomo, Melisa; Beltramino, Sofia; Loh, Watson; Romanini, Diana; et al.; A sustainable affinity partitioning process to recover papain from Carica papaya latex using alginate as macro-ligand; Elsevier Science; Separation and Purification Technology; 168; 8-2016; 168-1761383-5866CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.seppur.2016.05.025info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1383586616305032info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:23Zoai:ri.conicet.gov.ar:11336/52653instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:24.115CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A sustainable affinity partitioning process to recover papain from Carica papaya latex using alginate as macro-ligand
title A sustainable affinity partitioning process to recover papain from Carica papaya latex using alginate as macro-ligand
spellingShingle A sustainable affinity partitioning process to recover papain from Carica papaya latex using alginate as macro-ligand
Rocha, María Victoria
Affinity Partitioning
Alginate
Aqueous Two-Phase Systems
Papain
title_short A sustainable affinity partitioning process to recover papain from Carica papaya latex using alginate as macro-ligand
title_full A sustainable affinity partitioning process to recover papain from Carica papaya latex using alginate as macro-ligand
title_fullStr A sustainable affinity partitioning process to recover papain from Carica papaya latex using alginate as macro-ligand
title_full_unstemmed A sustainable affinity partitioning process to recover papain from Carica papaya latex using alginate as macro-ligand
title_sort A sustainable affinity partitioning process to recover papain from Carica papaya latex using alginate as macro-ligand
dc.creator.none.fl_str_mv Rocha, María Victoria
Di Giacomo, Melisa
Beltramino, Sofia
Loh, Watson
Romanini, Diana
Nerli, Bibiana Beatriz
author Rocha, María Victoria
author_facet Rocha, María Victoria
Di Giacomo, Melisa
Beltramino, Sofia
Loh, Watson
Romanini, Diana
Nerli, Bibiana Beatriz
author_role author
author2 Di Giacomo, Melisa
Beltramino, Sofia
Loh, Watson
Romanini, Diana
Nerli, Bibiana Beatriz
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Affinity Partitioning
Alginate
Aqueous Two-Phase Systems
Papain
topic Affinity Partitioning
Alginate
Aqueous Two-Phase Systems
Papain
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The role of the natural biodegradable polymer alginate (ALG) as affinity macro-ligand for papain (PAP) was evaluated in order to design a new sustainable two-phase affinity strategy for recovering this enzyme from Carica papaya latex. In presence of PAP, decreased values of intrinsic viscosity and hydrodynamic radius of ALG were observed. These results suggested a neutralizing effect of PAP on the polymer negative charges which caused a decrease of intra-chain repulsion forces in ALG molecules and a consequent shrinkage of the polymer size. Calorimetric measurements demonstrated a cooperative interaction between PAP and ALG which was enthalpy-entropically driven. When partitioned C. papaya latex into aqueous two-phase systems (ATPSs) formed by polyethylene glycol (PEG) of MW 8000 and the biodegradable salt sodium citrate pH 5.20, only 20% of PAP was recovered at the PEG-enriched phase with a purification factor (PF) of 2.48. The addition of ALG 0.1% (w/w) into the system doubled the PAP partition coefficient (Kp, PAP), showing the ability of this polymer to enhance the enzyme recovery at the PEG-enriched phase and therefore, increasing the extraction efficiency. The subsequent addition of calcium chloride at a final concentration of 80 mM allowed the precipitation of the target enzyme and the recovering of PEG phase for recycling. The overall process showed a PAP recovery of 72% and a PF of 2.41. The proposed strategy not only conserved all the advantages of the reported extractions with PEG/ammonium sulfate (or phosphate) ATPSs, i.e., low cost, scalability and integration of the clarification and extraction steps, but also allowed overcoming their main drawbacks, i.e., the separation of the target molecule from the phase-polymer and the environmental impact caused by the disposal of these salts.
Fil: Rocha, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Di Giacomo, Melisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Beltramino, Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Loh, Watson. State university of Campinas; Brasil
Fil: Romanini, Diana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Nerli, Bibiana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
description The role of the natural biodegradable polymer alginate (ALG) as affinity macro-ligand for papain (PAP) was evaluated in order to design a new sustainable two-phase affinity strategy for recovering this enzyme from Carica papaya latex. In presence of PAP, decreased values of intrinsic viscosity and hydrodynamic radius of ALG were observed. These results suggested a neutralizing effect of PAP on the polymer negative charges which caused a decrease of intra-chain repulsion forces in ALG molecules and a consequent shrinkage of the polymer size. Calorimetric measurements demonstrated a cooperative interaction between PAP and ALG which was enthalpy-entropically driven. When partitioned C. papaya latex into aqueous two-phase systems (ATPSs) formed by polyethylene glycol (PEG) of MW 8000 and the biodegradable salt sodium citrate pH 5.20, only 20% of PAP was recovered at the PEG-enriched phase with a purification factor (PF) of 2.48. The addition of ALG 0.1% (w/w) into the system doubled the PAP partition coefficient (Kp, PAP), showing the ability of this polymer to enhance the enzyme recovery at the PEG-enriched phase and therefore, increasing the extraction efficiency. The subsequent addition of calcium chloride at a final concentration of 80 mM allowed the precipitation of the target enzyme and the recovering of PEG phase for recycling. The overall process showed a PAP recovery of 72% and a PF of 2.41. The proposed strategy not only conserved all the advantages of the reported extractions with PEG/ammonium sulfate (or phosphate) ATPSs, i.e., low cost, scalability and integration of the clarification and extraction steps, but also allowed overcoming their main drawbacks, i.e., the separation of the target molecule from the phase-polymer and the environmental impact caused by the disposal of these salts.
publishDate 2016
dc.date.none.fl_str_mv 2016-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/52653
Rocha, María Victoria; Di Giacomo, Melisa; Beltramino, Sofia; Loh, Watson; Romanini, Diana; et al.; A sustainable affinity partitioning process to recover papain from Carica papaya latex using alginate as macro-ligand; Elsevier Science; Separation and Purification Technology; 168; 8-2016; 168-176
1383-5866
CONICET Digital
CONICET
url http://hdl.handle.net/11336/52653
identifier_str_mv Rocha, María Victoria; Di Giacomo, Melisa; Beltramino, Sofia; Loh, Watson; Romanini, Diana; et al.; A sustainable affinity partitioning process to recover papain from Carica papaya latex using alginate as macro-ligand; Elsevier Science; Separation and Purification Technology; 168; 8-2016; 168-176
1383-5866
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.seppur.2016.05.025
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1383586616305032
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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