Cloud point extraction based on non-ionic surfactants: An ecofriendly tool for recovering papain from papaya latex

Autores
Di Giacomo, Melisa; Bertoni, Fernando Ariel; Rocha, María Victoria; Nerli, Bibiana Beatriz; Rodriguez, Fernanda Mariana
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The potential use of cloud point extraction with aqueous micellar two-phase systems (AMTPS), formed by the non-ionic biodegradable surfactants Triton X-114 (TX-114) and Genapol X-080 (GX-080), to recover papain (PAP) from fresh papaya latex was evaluated. Binodal curves obtained for both surfactants, in absence and presence of PAP, showed cloud points below 40 °C, the PAP presence slightly favoring the phase separation phenomenon. Partitioning experiments showed that PAP migrated toward the micelle-poor phase independently of the surfactant and the working temperature assayed, this behavior being consistent with this enzyme hydrophilic character. PAP recoveries of over 60% in the poor-micelle phase were obtained when partitioned fresh papaya latex in both surfactant-based AMTPS. TX-114 systems (4.00% w/w, 33 °C) allowed recovering 66% of PAP with a purification factor of 4.6. On GX-080 systems (4.00% w/w, 42 °C) a yield of 78% PAP and a purification factor of 3.7 were achieved. This AMTPS also allowed the separation of PAP from reducing sugars present in papaya latex. These results show that TX-114 and GX-080-based AMTPS could be potentially used as simple and economical tools at recovering PAP from papaya latex.
Fil: Di Giacomo, Melisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Bertoni, Fernando Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Rocha, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Nerli, Bibiana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Rodriguez, Fernanda Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Materia
AQUEOUS TWO-PHASE SYSTEMS
BIODEGRADABLE SURFACTANTS
CLOUD POINT EXTRACTION
MICELLAR SYSTEMS
PAPAIN
SUSTAINABLE METHODS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/211599
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/211599
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cloud point extraction based on non-ionic surfactants: An ecofriendly tool for recovering papain from papaya latexDi Giacomo, MelisaBertoni, Fernando ArielRocha, María VictoriaNerli, Bibiana BeatrizRodriguez, Fernanda MarianaAQUEOUS TWO-PHASE SYSTEMSBIODEGRADABLE SURFACTANTSCLOUD POINT EXTRACTIONMICELLAR SYSTEMSPAPAINSUSTAINABLE METHODShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The potential use of cloud point extraction with aqueous micellar two-phase systems (AMTPS), formed by the non-ionic biodegradable surfactants Triton X-114 (TX-114) and Genapol X-080 (GX-080), to recover papain (PAP) from fresh papaya latex was evaluated. Binodal curves obtained for both surfactants, in absence and presence of PAP, showed cloud points below 40 °C, the PAP presence slightly favoring the phase separation phenomenon. Partitioning experiments showed that PAP migrated toward the micelle-poor phase independently of the surfactant and the working temperature assayed, this behavior being consistent with this enzyme hydrophilic character. PAP recoveries of over 60% in the poor-micelle phase were obtained when partitioned fresh papaya latex in both surfactant-based AMTPS. TX-114 systems (4.00% w/w, 33 °C) allowed recovering 66% of PAP with a purification factor of 4.6. On GX-080 systems (4.00% w/w, 42 °C) a yield of 78% PAP and a purification factor of 3.7 were achieved. This AMTPS also allowed the separation of PAP from reducing sugars present in papaya latex. These results show that TX-114 and GX-080-based AMTPS could be potentially used as simple and economical tools at recovering PAP from papaya latex.Fil: Di Giacomo, Melisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; ArgentinaFil: Bertoni, Fernando Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; ArgentinaFil: Rocha, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; ArgentinaFil: Nerli, Bibiana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; ArgentinaFil: Rodriguez, Fernanda Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; ArgentinaElsevier2022-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/211599Di Giacomo, Melisa; Bertoni, Fernando Ariel; Rocha, María Victoria; Nerli, Bibiana Beatriz; Rodriguez, Fernanda Mariana; Cloud point extraction based on non-ionic surfactants: An ecofriendly tool for recovering papain from papaya latex; Elsevier; Journal of Environmental Chemical Engineering; 10; 6; 12-2022; 1-422213-3437CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2213343722016359info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jece.2022.108762info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:09Zoai:ri.conicet.gov.ar:11336/211599instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:09.631CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cloud point extraction based on non-ionic surfactants: An ecofriendly tool for recovering papain from papaya latex
title Cloud point extraction based on non-ionic surfactants: An ecofriendly tool for recovering papain from papaya latex
spellingShingle Cloud point extraction based on non-ionic surfactants: An ecofriendly tool for recovering papain from papaya latex
Di Giacomo, Melisa
AQUEOUS TWO-PHASE SYSTEMS
BIODEGRADABLE SURFACTANTS
CLOUD POINT EXTRACTION
MICELLAR SYSTEMS
PAPAIN
SUSTAINABLE METHODS
title_short Cloud point extraction based on non-ionic surfactants: An ecofriendly tool for recovering papain from papaya latex
title_full Cloud point extraction based on non-ionic surfactants: An ecofriendly tool for recovering papain from papaya latex
title_fullStr Cloud point extraction based on non-ionic surfactants: An ecofriendly tool for recovering papain from papaya latex
title_full_unstemmed Cloud point extraction based on non-ionic surfactants: An ecofriendly tool for recovering papain from papaya latex
title_sort Cloud point extraction based on non-ionic surfactants: An ecofriendly tool for recovering papain from papaya latex
dc.creator.none.fl_str_mv Di Giacomo, Melisa
Bertoni, Fernando Ariel
Rocha, María Victoria
Nerli, Bibiana Beatriz
Rodriguez, Fernanda Mariana
author Di Giacomo, Melisa
author_facet Di Giacomo, Melisa
Bertoni, Fernando Ariel
Rocha, María Victoria
Nerli, Bibiana Beatriz
Rodriguez, Fernanda Mariana
author_role author
author2 Bertoni, Fernando Ariel
Rocha, María Victoria
Nerli, Bibiana Beatriz
Rodriguez, Fernanda Mariana
author2_role author
author
author
author
dc.subject.none.fl_str_mv AQUEOUS TWO-PHASE SYSTEMS
BIODEGRADABLE SURFACTANTS
CLOUD POINT EXTRACTION
MICELLAR SYSTEMS
PAPAIN
SUSTAINABLE METHODS
topic AQUEOUS TWO-PHASE SYSTEMS
BIODEGRADABLE SURFACTANTS
CLOUD POINT EXTRACTION
MICELLAR SYSTEMS
PAPAIN
SUSTAINABLE METHODS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The potential use of cloud point extraction with aqueous micellar two-phase systems (AMTPS), formed by the non-ionic biodegradable surfactants Triton X-114 (TX-114) and Genapol X-080 (GX-080), to recover papain (PAP) from fresh papaya latex was evaluated. Binodal curves obtained for both surfactants, in absence and presence of PAP, showed cloud points below 40 °C, the PAP presence slightly favoring the phase separation phenomenon. Partitioning experiments showed that PAP migrated toward the micelle-poor phase independently of the surfactant and the working temperature assayed, this behavior being consistent with this enzyme hydrophilic character. PAP recoveries of over 60% in the poor-micelle phase were obtained when partitioned fresh papaya latex in both surfactant-based AMTPS. TX-114 systems (4.00% w/w, 33 °C) allowed recovering 66% of PAP with a purification factor of 4.6. On GX-080 systems (4.00% w/w, 42 °C) a yield of 78% PAP and a purification factor of 3.7 were achieved. This AMTPS also allowed the separation of PAP from reducing sugars present in papaya latex. These results show that TX-114 and GX-080-based AMTPS could be potentially used as simple and economical tools at recovering PAP from papaya latex.
Fil: Di Giacomo, Melisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Bertoni, Fernando Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Rocha, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Nerli, Bibiana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Rodriguez, Fernanda Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
description The potential use of cloud point extraction with aqueous micellar two-phase systems (AMTPS), formed by the non-ionic biodegradable surfactants Triton X-114 (TX-114) and Genapol X-080 (GX-080), to recover papain (PAP) from fresh papaya latex was evaluated. Binodal curves obtained for both surfactants, in absence and presence of PAP, showed cloud points below 40 °C, the PAP presence slightly favoring the phase separation phenomenon. Partitioning experiments showed that PAP migrated toward the micelle-poor phase independently of the surfactant and the working temperature assayed, this behavior being consistent with this enzyme hydrophilic character. PAP recoveries of over 60% in the poor-micelle phase were obtained when partitioned fresh papaya latex in both surfactant-based AMTPS. TX-114 systems (4.00% w/w, 33 °C) allowed recovering 66% of PAP with a purification factor of 4.6. On GX-080 systems (4.00% w/w, 42 °C) a yield of 78% PAP and a purification factor of 3.7 were achieved. This AMTPS also allowed the separation of PAP from reducing sugars present in papaya latex. These results show that TX-114 and GX-080-based AMTPS could be potentially used as simple and economical tools at recovering PAP from papaya latex.
publishDate 2022
dc.date.none.fl_str_mv 2022-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/211599
Di Giacomo, Melisa; Bertoni, Fernando Ariel; Rocha, María Victoria; Nerli, Bibiana Beatriz; Rodriguez, Fernanda Mariana; Cloud point extraction based on non-ionic surfactants: An ecofriendly tool for recovering papain from papaya latex; Elsevier; Journal of Environmental Chemical Engineering; 10; 6; 12-2022; 1-42
2213-3437
CONICET Digital
CONICET
url http://hdl.handle.net/11336/211599
identifier_str_mv Di Giacomo, Melisa; Bertoni, Fernando Ariel; Rocha, María Victoria; Nerli, Bibiana Beatriz; Rodriguez, Fernanda Mariana; Cloud point extraction based on non-ionic surfactants: An ecofriendly tool for recovering papain from papaya latex; Elsevier; Journal of Environmental Chemical Engineering; 10; 6; 12-2022; 1-42
2213-3437
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2213343722016359
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jece.2022.108762
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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