Sodium tetrathionate effect on papain purification from different Carica papaya latex crude extracts

Autores
Llerena Suster, Carlos Rafael; Priolo, Nora Silvia; Morcelle del Valle, Susana Raquel
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Papain from latex of Carica papaya was purified up to MALDI TOF mass spectrometry homogeinity by salt precipitation from two different crude extract sources: a refined preparation obtained in our laboratory and a commercial one. Sodium tetrathionate was tested in the purification process to preserve the enzymatic activity of the peptidase. Purification was checked by SDS PAGE and cation exchange chromatography, using commercial pure papain as standard for a rapid comparison. The best purification yields (3.4 %) were obtained in presence of 30 mM sodium tetrathionate for the crude extract prepared in our laboratory. The described purification method proved to be robust and reliable to obtain pure papain in a preparative scale.
Fil: Llerena Suster, Carlos Rafael. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Priolo, Nora Silvia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina
Fil: Morcelle del Valle, Susana Raquel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Materia
PAPAIN
PLANT CYSTEINE PEPTIDASES
PROTEIN PURIFICATION
SODIUM TETRATHIONATE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/101835
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Sodium tetrathionate effect on papain purification from different Carica papaya latex crude extractsLlerena Suster, Carlos RafaelPriolo, Nora SilviaMorcelle del Valle, Susana RaquelPAPAINPLANT CYSTEINE PEPTIDASESPROTEIN PURIFICATIONSODIUM TETRATHIONATEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Papain from latex of Carica papaya was purified up to MALDI TOF mass spectrometry homogeinity by salt precipitation from two different crude extract sources: a refined preparation obtained in our laboratory and a commercial one. Sodium tetrathionate was tested in the purification process to preserve the enzymatic activity of the peptidase. Purification was checked by SDS PAGE and cation exchange chromatography, using commercial pure papain as standard for a rapid comparison. The best purification yields (3.4 %) were obtained in presence of 30 mM sodium tetrathionate for the crude extract prepared in our laboratory. The described purification method proved to be robust and reliable to obtain pure papain in a preparative scale.Fil: Llerena Suster, Carlos Rafael. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Priolo, Nora Silvia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; ArgentinaFil: Morcelle del Valle, Susana Raquel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaTaylor & Francis2011-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/101835Llerena Suster, Carlos Rafael; Priolo, Nora Silvia; Morcelle del Valle, Susana Raquel; Sodium tetrathionate effect on papain purification from different Carica papaya latex crude extracts; Taylor & Francis; Preparative Biochemistry and Biotechnology; 41; 4-2011; 107-1211082-6068CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/10826068.2011.544230info:eu-repo/semantics/altIdentifier/doi/10.1080/10826068.2011.544230info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:56:16Zoai:ri.conicet.gov.ar:11336/101835instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:56:16.631CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sodium tetrathionate effect on papain purification from different Carica papaya latex crude extracts
title Sodium tetrathionate effect on papain purification from different Carica papaya latex crude extracts
spellingShingle Sodium tetrathionate effect on papain purification from different Carica papaya latex crude extracts
Llerena Suster, Carlos Rafael
PAPAIN
PLANT CYSTEINE PEPTIDASES
PROTEIN PURIFICATION
SODIUM TETRATHIONATE
title_short Sodium tetrathionate effect on papain purification from different Carica papaya latex crude extracts
title_full Sodium tetrathionate effect on papain purification from different Carica papaya latex crude extracts
title_fullStr Sodium tetrathionate effect on papain purification from different Carica papaya latex crude extracts
title_full_unstemmed Sodium tetrathionate effect on papain purification from different Carica papaya latex crude extracts
title_sort Sodium tetrathionate effect on papain purification from different Carica papaya latex crude extracts
dc.creator.none.fl_str_mv Llerena Suster, Carlos Rafael
Priolo, Nora Silvia
Morcelle del Valle, Susana Raquel
author Llerena Suster, Carlos Rafael
author_facet Llerena Suster, Carlos Rafael
Priolo, Nora Silvia
Morcelle del Valle, Susana Raquel
author_role author
author2 Priolo, Nora Silvia
Morcelle del Valle, Susana Raquel
author2_role author
author
dc.subject.none.fl_str_mv PAPAIN
PLANT CYSTEINE PEPTIDASES
PROTEIN PURIFICATION
SODIUM TETRATHIONATE
topic PAPAIN
PLANT CYSTEINE PEPTIDASES
PROTEIN PURIFICATION
SODIUM TETRATHIONATE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Papain from latex of Carica papaya was purified up to MALDI TOF mass spectrometry homogeinity by salt precipitation from two different crude extract sources: a refined preparation obtained in our laboratory and a commercial one. Sodium tetrathionate was tested in the purification process to preserve the enzymatic activity of the peptidase. Purification was checked by SDS PAGE and cation exchange chromatography, using commercial pure papain as standard for a rapid comparison. The best purification yields (3.4 %) were obtained in presence of 30 mM sodium tetrathionate for the crude extract prepared in our laboratory. The described purification method proved to be robust and reliable to obtain pure papain in a preparative scale.
Fil: Llerena Suster, Carlos Rafael. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Priolo, Nora Silvia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina
Fil: Morcelle del Valle, Susana Raquel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
description Papain from latex of Carica papaya was purified up to MALDI TOF mass spectrometry homogeinity by salt precipitation from two different crude extract sources: a refined preparation obtained in our laboratory and a commercial one. Sodium tetrathionate was tested in the purification process to preserve the enzymatic activity of the peptidase. Purification was checked by SDS PAGE and cation exchange chromatography, using commercial pure papain as standard for a rapid comparison. The best purification yields (3.4 %) were obtained in presence of 30 mM sodium tetrathionate for the crude extract prepared in our laboratory. The described purification method proved to be robust and reliable to obtain pure papain in a preparative scale.
publishDate 2011
dc.date.none.fl_str_mv 2011-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/101835
Llerena Suster, Carlos Rafael; Priolo, Nora Silvia; Morcelle del Valle, Susana Raquel; Sodium tetrathionate effect on papain purification from different Carica papaya latex crude extracts; Taylor & Francis; Preparative Biochemistry and Biotechnology; 41; 4-2011; 107-121
1082-6068
CONICET Digital
CONICET
url http://hdl.handle.net/11336/101835
identifier_str_mv Llerena Suster, Carlos Rafael; Priolo, Nora Silvia; Morcelle del Valle, Susana Raquel; Sodium tetrathionate effect on papain purification from different Carica papaya latex crude extracts; Taylor & Francis; Preparative Biochemistry and Biotechnology; 41; 4-2011; 107-121
1082-6068
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/10826068.2011.544230
info:eu-repo/semantics/altIdentifier/doi/10.1080/10826068.2011.544230
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.001348

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