Pancreatic serine protease extraction by affinity partition using a free triazine dye

Autores
Rocha, María Victoria; Romanini, Diana; Nerli, Bibiana Beatriz; Tubio, Gisela
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Affinity partitioning combines the partitioning behavior of biological macromolecules in aqueous two phase systems with the principle of biorecognition. Among the numerous substances that have been evaluated as ligands, the reactive dyes constitute a group of low cost textile dyes which have proved to act as biomimetic ligands formany enzymes. The ability of reactive yellow 2 (RY2)to interact with trypsin (TRP) and chymotrypsin (ChTRP) and its behavior in aqueous two-phase systems formed by polyethylene glycol (PEG) and sodium citrate (NaCit) ? were investigated. Different variables such as PEG molecular weight, tie line length and dye concentration were analyzed. RY2 showed to bind specifically to both TRP and ChTRP with affinity constants near to 10 3M−1. Its partition equilibrium is practically displaced to the top phase in systems formed by PEG of differentmolecular weight. Addition ofthis dye to PEG 8000/NaCit systems until a final concentration of 0.196% (w/w) induced an increase in TRP and ChTRP partition coefficients of at least 2 times over that in the absence of the ligand. These findings demonstrate that RY2 fulfils all the requirements to be considered as an affinity ligand in aqueous two-phase partitioning of TRP and ChTRP.
Fil: Rocha, María Victoria. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Romanini, Diana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; Argentina
Fil: Nerli, Bibiana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; Argentina
Fil: Tubio, Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; Argentina
Materia
AFFINITY PARTITIONING
AQUEOUS TWO-PHASE SYSTEMS
TRYPSIN
FREE REACTIVE DYE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/271661
Acceder
id CONICETDig_0287bfded7e5b4ac305217e52199906f
oai_identifier_str oai:ri.conicet.gov.ar:11336/271661
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Pancreatic serine protease extraction by affinity partition using a free triazine dyeRocha, María VictoriaRomanini, DianaNerli, Bibiana BeatrizTubio, GiselaAFFINITY PARTITIONINGAQUEOUS TWO-PHASE SYSTEMSTRYPSINFREE REACTIVE DYEhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Affinity partitioning combines the partitioning behavior of biological macromolecules in aqueous two phase systems with the principle of biorecognition. Among the numerous substances that have been evaluated as ligands, the reactive dyes constitute a group of low cost textile dyes which have proved to act as biomimetic ligands formany enzymes. The ability of reactive yellow 2 (RY2)to interact with trypsin (TRP) and chymotrypsin (ChTRP) and its behavior in aqueous two-phase systems formed by polyethylene glycol (PEG) and sodium citrate (NaCit) ? were investigated. Different variables such as PEG molecular weight, tie line length and dye concentration were analyzed. RY2 showed to bind specifically to both TRP and ChTRP with affinity constants near to 10 3M−1. Its partition equilibrium is practically displaced to the top phase in systems formed by PEG of differentmolecular weight. Addition ofthis dye to PEG 8000/NaCit systems until a final concentration of 0.196% (w/w) induced an increase in TRP and ChTRP partition coefficients of at least 2 times over that in the absence of the ligand. These findings demonstrate that RY2 fulfils all the requirements to be considered as an affinity ligand in aqueous two-phase partitioning of TRP and ChTRP.Fil: Rocha, María Victoria. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Romanini, Diana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; ArgentinaFil: Nerli, Bibiana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; ArgentinaFil: Tubio, Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; ArgentinaElsevier Science2012-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/271661Rocha, María Victoria; Romanini, Diana; Nerli, Bibiana Beatriz; Tubio, Gisela; Pancreatic serine protease extraction by affinity partition using a free triazine dye; Elsevier Science; International Journal of Biological Macromolecules; 50; 2; 3-2012; 303-3090141-8130CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0141813011004740info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2011.12.013info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:32:46Zoai:ri.conicet.gov.ar:11336/271661instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:32:47.286CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Pancreatic serine protease extraction by affinity partition using a free triazine dye
title Pancreatic serine protease extraction by affinity partition using a free triazine dye
spellingShingle Pancreatic serine protease extraction by affinity partition using a free triazine dye
Rocha, María Victoria
AFFINITY PARTITIONING
AQUEOUS TWO-PHASE SYSTEMS
TRYPSIN
FREE REACTIVE DYE
title_short Pancreatic serine protease extraction by affinity partition using a free triazine dye
title_full Pancreatic serine protease extraction by affinity partition using a free triazine dye
title_fullStr Pancreatic serine protease extraction by affinity partition using a free triazine dye
title_full_unstemmed Pancreatic serine protease extraction by affinity partition using a free triazine dye
title_sort Pancreatic serine protease extraction by affinity partition using a free triazine dye
dc.creator.none.fl_str_mv Rocha, María Victoria
Romanini, Diana
Nerli, Bibiana Beatriz
Tubio, Gisela
author Rocha, María Victoria
author_facet Rocha, María Victoria
Romanini, Diana
Nerli, Bibiana Beatriz
Tubio, Gisela
author_role author
author2 Romanini, Diana
Nerli, Bibiana Beatriz
Tubio, Gisela
author2_role author
author
author
dc.subject.none.fl_str_mv AFFINITY PARTITIONING
AQUEOUS TWO-PHASE SYSTEMS
TRYPSIN
FREE REACTIVE DYE
topic AFFINITY PARTITIONING
AQUEOUS TWO-PHASE SYSTEMS
TRYPSIN
FREE REACTIVE DYE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Affinity partitioning combines the partitioning behavior of biological macromolecules in aqueous two phase systems with the principle of biorecognition. Among the numerous substances that have been evaluated as ligands, the reactive dyes constitute a group of low cost textile dyes which have proved to act as biomimetic ligands formany enzymes. The ability of reactive yellow 2 (RY2)to interact with trypsin (TRP) and chymotrypsin (ChTRP) and its behavior in aqueous two-phase systems formed by polyethylene glycol (PEG) and sodium citrate (NaCit) ? were investigated. Different variables such as PEG molecular weight, tie line length and dye concentration were analyzed. RY2 showed to bind specifically to both TRP and ChTRP with affinity constants near to 10 3M−1. Its partition equilibrium is practically displaced to the top phase in systems formed by PEG of differentmolecular weight. Addition ofthis dye to PEG 8000/NaCit systems until a final concentration of 0.196% (w/w) induced an increase in TRP and ChTRP partition coefficients of at least 2 times over that in the absence of the ligand. These findings demonstrate that RY2 fulfils all the requirements to be considered as an affinity ligand in aqueous two-phase partitioning of TRP and ChTRP.
Fil: Rocha, María Victoria. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Romanini, Diana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; Argentina
Fil: Nerli, Bibiana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; Argentina
Fil: Tubio, Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; Argentina
description Affinity partitioning combines the partitioning behavior of biological macromolecules in aqueous two phase systems with the principle of biorecognition. Among the numerous substances that have been evaluated as ligands, the reactive dyes constitute a group of low cost textile dyes which have proved to act as biomimetic ligands formany enzymes. The ability of reactive yellow 2 (RY2)to interact with trypsin (TRP) and chymotrypsin (ChTRP) and its behavior in aqueous two-phase systems formed by polyethylene glycol (PEG) and sodium citrate (NaCit) ? were investigated. Different variables such as PEG molecular weight, tie line length and dye concentration were analyzed. RY2 showed to bind specifically to both TRP and ChTRP with affinity constants near to 10 3M−1. Its partition equilibrium is practically displaced to the top phase in systems formed by PEG of differentmolecular weight. Addition ofthis dye to PEG 8000/NaCit systems until a final concentration of 0.196% (w/w) induced an increase in TRP and ChTRP partition coefficients of at least 2 times over that in the absence of the ligand. These findings demonstrate that RY2 fulfils all the requirements to be considered as an affinity ligand in aqueous two-phase partitioning of TRP and ChTRP.
publishDate 2012
dc.date.none.fl_str_mv 2012-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/271661
Rocha, María Victoria; Romanini, Diana; Nerli, Bibiana Beatriz; Tubio, Gisela; Pancreatic serine protease extraction by affinity partition using a free triazine dye; Elsevier Science; International Journal of Biological Macromolecules; 50; 2; 3-2012; 303-309
0141-8130
CONICET Digital
CONICET
url http://hdl.handle.net/11336/271661
identifier_str_mv Rocha, María Victoria; Romanini, Diana; Nerli, Bibiana Beatriz; Tubio, Gisela; Pancreatic serine protease extraction by affinity partition using a free triazine dye; Elsevier Science; International Journal of Biological Macromolecules; 50; 2; 3-2012; 303-309
0141-8130
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0141813011004740
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2011.12.013
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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