Distribution and characterization of corazonin in the central nervous system of Triatoma infestans (Insecta: Heteroptera)

Autores
Settembrini, Beatriz Patricia; De Pasquale, Daniela; Postal, Melissa; Pinto, Paulo M.; Carlini, Célia Regina R S; Villar, Marcelo Jose
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The distribution of corazonin in the central nervous system of the heteropteran insect Triatoma infestans was studied by immunohistochemistry. The presence of corazonin isoforms was investigated using MALDI-TOF mass spectrometry in samples containing the brain, the subesophageal ganglion, the corpora cardiaca-corpus allatum complex and the anterior part of the aorta. Several groups of immunopositive perikarya were detected in the brain, the subesophageal ganglion and the thoracic ganglia. Regarding the brain, three clusters were observed in the protocerebrum. One of these clusters was formed by somata located near the entrance of the ocellar nerves whose fibers supplied the aorta and the corpora cardiaca. The remaining groups of the protocerebrum were located in the lateral soma cortex and at the boundary of the protocerebrum with the optic lobe. The optic lobe housed immunoreactive somata in the medial soma layer of the lobula and at the level of the first optic chiasma. The neuropils of the deutocerebrum and the tritocerebrum were immunostained, but no immunoreactive perikarya were detected. In the subesophageal ganglion, immunostained somata were found in the soma layers of the mandibular and labial neuromeres, whereas in the mesothoracic ganglionic mass, they were observed in the mesothoracic, metathoracic and abdominal neuromeres. Immunostained neurites were also found in the esophageal wall. The distribution pattern of corazonin like immunoreactivity in the central nervous system of this species suggests that corazonin may act as a neurohormone. Mass spectrometric analysis revealed that [Arg 7]-corazonin was the only isoform of the neuropeptide present in T. infestans tissue samples.
Fil: Settembrini, Beatriz Patricia. Universidad Austral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales “Bernardino Rivadavia”; Argentina
Fil: De Pasquale, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales “Bernardino Rivadavia”; Argentina
Fil: Postal, Melissa. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Pinto, Paulo M.. Universidade de Caxias do Sul; Brasil
Fil: Carlini, Célia Regina R S. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Villar, Marcelo Jose. Universidad Austral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Chagas Disease
Corazonin
Immunohistochemistry
Insect Central Nervous System
Mass Spectrometry
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/69017
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/69017
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Distribution and characterization of corazonin in the central nervous system of Triatoma infestans (Insecta: Heteroptera)Settembrini, Beatriz PatriciaDe Pasquale, DanielaPostal, MelissaPinto, Paulo M.Carlini, Célia Regina R SVillar, Marcelo JoseChagas DiseaseCorazoninImmunohistochemistryInsect Central Nervous SystemMass Spectrometryhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The distribution of corazonin in the central nervous system of the heteropteran insect Triatoma infestans was studied by immunohistochemistry. The presence of corazonin isoforms was investigated using MALDI-TOF mass spectrometry in samples containing the brain, the subesophageal ganglion, the corpora cardiaca-corpus allatum complex and the anterior part of the aorta. Several groups of immunopositive perikarya were detected in the brain, the subesophageal ganglion and the thoracic ganglia. Regarding the brain, three clusters were observed in the protocerebrum. One of these clusters was formed by somata located near the entrance of the ocellar nerves whose fibers supplied the aorta and the corpora cardiaca. The remaining groups of the protocerebrum were located in the lateral soma cortex and at the boundary of the protocerebrum with the optic lobe. The optic lobe housed immunoreactive somata in the medial soma layer of the lobula and at the level of the first optic chiasma. The neuropils of the deutocerebrum and the tritocerebrum were immunostained, but no immunoreactive perikarya were detected. In the subesophageal ganglion, immunostained somata were found in the soma layers of the mandibular and labial neuromeres, whereas in the mesothoracic ganglionic mass, they were observed in the mesothoracic, metathoracic and abdominal neuromeres. Immunostained neurites were also found in the esophageal wall. The distribution pattern of corazonin like immunoreactivity in the central nervous system of this species suggests that corazonin may act as a neurohormone. Mass spectrometric analysis revealed that [Arg 7]-corazonin was the only isoform of the neuropeptide present in T. infestans tissue samples.Fil: Settembrini, Beatriz Patricia. Universidad Austral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales “Bernardino Rivadavia”; ArgentinaFil: De Pasquale, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales “Bernardino Rivadavia”; ArgentinaFil: Postal, Melissa. Universidade Federal do Rio Grande do Sul; BrasilFil: Pinto, Paulo M.. Universidade de Caxias do Sul; BrasilFil: Carlini, Célia Regina R S. Universidade Federal do Rio Grande do Sul; BrasilFil: Villar, Marcelo Jose. Universidad Austral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science2011-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/69017Settembrini, Beatriz Patricia; De Pasquale, Daniela; Postal, Melissa; Pinto, Paulo M.; Carlini, Célia Regina R S; et al.; Distribution and characterization of corazonin in the central nervous system of Triatoma infestans (Insecta: Heteroptera); Elsevier Science; Peptides; 32; 3; 3-2011; 461-4680196-9781CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.peptides.2010.10.020info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0196978110004730info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:36:10Zoai:ri.conicet.gov.ar:11336/69017instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:36:11.0CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Distribution and characterization of corazonin in the central nervous system of Triatoma infestans (Insecta: Heteroptera)
title Distribution and characterization of corazonin in the central nervous system of Triatoma infestans (Insecta: Heteroptera)
spellingShingle Distribution and characterization of corazonin in the central nervous system of Triatoma infestans (Insecta: Heteroptera)
Settembrini, Beatriz Patricia
Chagas Disease
Corazonin
Immunohistochemistry
Insect Central Nervous System
Mass Spectrometry
title_short Distribution and characterization of corazonin in the central nervous system of Triatoma infestans (Insecta: Heteroptera)
title_full Distribution and characterization of corazonin in the central nervous system of Triatoma infestans (Insecta: Heteroptera)
title_fullStr Distribution and characterization of corazonin in the central nervous system of Triatoma infestans (Insecta: Heteroptera)
title_full_unstemmed Distribution and characterization of corazonin in the central nervous system of Triatoma infestans (Insecta: Heteroptera)
title_sort Distribution and characterization of corazonin in the central nervous system of Triatoma infestans (Insecta: Heteroptera)
dc.creator.none.fl_str_mv Settembrini, Beatriz Patricia
De Pasquale, Daniela
Postal, Melissa
Pinto, Paulo M.
Carlini, Célia Regina R S
Villar, Marcelo Jose
author Settembrini, Beatriz Patricia
author_facet Settembrini, Beatriz Patricia
De Pasquale, Daniela
Postal, Melissa
Pinto, Paulo M.
Carlini, Célia Regina R S
Villar, Marcelo Jose
author_role author
author2 De Pasquale, Daniela
Postal, Melissa
Pinto, Paulo M.
Carlini, Célia Regina R S
Villar, Marcelo Jose
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Chagas Disease
Corazonin
Immunohistochemistry
Insect Central Nervous System
Mass Spectrometry
topic Chagas Disease
Corazonin
Immunohistochemistry
Insect Central Nervous System
Mass Spectrometry
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The distribution of corazonin in the central nervous system of the heteropteran insect Triatoma infestans was studied by immunohistochemistry. The presence of corazonin isoforms was investigated using MALDI-TOF mass spectrometry in samples containing the brain, the subesophageal ganglion, the corpora cardiaca-corpus allatum complex and the anterior part of the aorta. Several groups of immunopositive perikarya were detected in the brain, the subesophageal ganglion and the thoracic ganglia. Regarding the brain, three clusters were observed in the protocerebrum. One of these clusters was formed by somata located near the entrance of the ocellar nerves whose fibers supplied the aorta and the corpora cardiaca. The remaining groups of the protocerebrum were located in the lateral soma cortex and at the boundary of the protocerebrum with the optic lobe. The optic lobe housed immunoreactive somata in the medial soma layer of the lobula and at the level of the first optic chiasma. The neuropils of the deutocerebrum and the tritocerebrum were immunostained, but no immunoreactive perikarya were detected. In the subesophageal ganglion, immunostained somata were found in the soma layers of the mandibular and labial neuromeres, whereas in the mesothoracic ganglionic mass, they were observed in the mesothoracic, metathoracic and abdominal neuromeres. Immunostained neurites were also found in the esophageal wall. The distribution pattern of corazonin like immunoreactivity in the central nervous system of this species suggests that corazonin may act as a neurohormone. Mass spectrometric analysis revealed that [Arg 7]-corazonin was the only isoform of the neuropeptide present in T. infestans tissue samples.
Fil: Settembrini, Beatriz Patricia. Universidad Austral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales “Bernardino Rivadavia”; Argentina
Fil: De Pasquale, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales “Bernardino Rivadavia”; Argentina
Fil: Postal, Melissa. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Pinto, Paulo M.. Universidade de Caxias do Sul; Brasil
Fil: Carlini, Célia Regina R S. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Villar, Marcelo Jose. Universidad Austral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The distribution of corazonin in the central nervous system of the heteropteran insect Triatoma infestans was studied by immunohistochemistry. The presence of corazonin isoforms was investigated using MALDI-TOF mass spectrometry in samples containing the brain, the subesophageal ganglion, the corpora cardiaca-corpus allatum complex and the anterior part of the aorta. Several groups of immunopositive perikarya were detected in the brain, the subesophageal ganglion and the thoracic ganglia. Regarding the brain, three clusters were observed in the protocerebrum. One of these clusters was formed by somata located near the entrance of the ocellar nerves whose fibers supplied the aorta and the corpora cardiaca. The remaining groups of the protocerebrum were located in the lateral soma cortex and at the boundary of the protocerebrum with the optic lobe. The optic lobe housed immunoreactive somata in the medial soma layer of the lobula and at the level of the first optic chiasma. The neuropils of the deutocerebrum and the tritocerebrum were immunostained, but no immunoreactive perikarya were detected. In the subesophageal ganglion, immunostained somata were found in the soma layers of the mandibular and labial neuromeres, whereas in the mesothoracic ganglionic mass, they were observed in the mesothoracic, metathoracic and abdominal neuromeres. Immunostained neurites were also found in the esophageal wall. The distribution pattern of corazonin like immunoreactivity in the central nervous system of this species suggests that corazonin may act as a neurohormone. Mass spectrometric analysis revealed that [Arg 7]-corazonin was the only isoform of the neuropeptide present in T. infestans tissue samples.
publishDate 2011
dc.date.none.fl_str_mv 2011-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/69017
Settembrini, Beatriz Patricia; De Pasquale, Daniela; Postal, Melissa; Pinto, Paulo M.; Carlini, Célia Regina R S; et al.; Distribution and characterization of corazonin in the central nervous system of Triatoma infestans (Insecta: Heteroptera); Elsevier Science; Peptides; 32; 3; 3-2011; 461-468
0196-9781
CONICET Digital
CONICET
url http://hdl.handle.net/11336/69017
identifier_str_mv Settembrini, Beatriz Patricia; De Pasquale, Daniela; Postal, Melissa; Pinto, Paulo M.; Carlini, Célia Regina R S; et al.; Distribution and characterization of corazonin in the central nervous system of Triatoma infestans (Insecta: Heteroptera); Elsevier Science; Peptides; 32; 3; 3-2011; 461-468
0196-9781
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.peptides.2010.10.020
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0196978110004730
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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