Increased glucose metabolic activity is associated with CD4+ T-cell activation and depletion during chronic HIV infection

Autores
Palmer, Clovis S.; Ostrowski, Matias; Gouillou, Maelenn; Tsai, Louis; Yu, Di; Zhou, Jingling; Henstridge, Darren C.; Maisa, Anna; Hearps, Anna C.; Lewin, Sharon R.; Landay, Alan; Jaworowski, Anthony; McCune, Joseph M.; Crowe, Suzanne M.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objectives: Glucose metabolism plays a fundamental role in supporting the growth, proliferation and effector functions of T cells. We investigated the impact of HIV infection on key processes that regulate glucose uptake and metabolism in primary CD4+ and CD8+ T cells. Design and methods: Thirty-eight HIV-infected treatment-naive, 35 HIV+/ combination antiretroviral therapy, seven HIV+ long-term nonprogressors and 25 HIV control individuals were studied. Basal markers of glycolysis [e.g. glucose transporter-1 (Glut1) expression, glucose uptake, intracellular glucose-6-phosphate, and L-lactate] were measured in T cells. The cellular markers of immune activation, CD38 and HLADR, were measured by flow cytometry. Results: The surface expression of the Glut1 is up-regulated in CD4+ T cells in HIVinfected patients compared with uninfected controls. The percentage of circulating CD4+Glut1+ T cells was significantly increased in HIV-infected patients and was not restored to normal levels following combination antiretroviral therapy. Basal markers of glycolysis were significantly higher in CD4+Glut1+ T cells compared to CD4+Glut1- T cells. The proportion of CD4+Glut1+ T cells correlated positively with the expression of the cellular activation marker, HLA-DR, on total CD4+ T cells, but inversely with the absolute CD4+ T-cell count irrespective of HIV treatment status. Conclusion: Our data suggest that Glut1 is a potentially novel and functional marker of CD4+ T-cell activation during HIV infection. In addition, Glut1 expression on CD4+ T cells may be exploited as a prognostic marker for CD4+ T-cell loss during HIV disease progression.
Fil: Palmer, Clovis S.. Burnet Institute; Australia
Fil: Ostrowski, Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
Fil: Gouillou, Maelenn. Burnet Institute; Australia
Fil: Tsai, Louis. Monash University; Australia
Fil: Yu, Di. Monash University; Australia
Fil: Zhou, Jingling. Burnet Institute; Australia
Fil: Henstridge, Darren C.. Baker IDI Heart and Diabetes Institute; Australia
Fil: Maisa, Anna. Burnet Institute; Australia
Fil: Hearps, Anna C.. Burnet Institute; Australia. Monash University; Australia
Fil: Lewin, Sharon R.. Burnet Institute; Australia. Monash University; Australia. The Alfred Hospital; Australia
Fil: Landay, Alan. Rush University Medical Center; Estados Unidos
Fil: Jaworowski, Anthony. Burnet Institute; Australia. Monash University; Australia
Fil: McCune, Joseph M.. University of California; Estados Unidos
Fil: Crowe, Suzanne M.. Burnet Institute; Australia. Monash University; Australia. The Alfred Hospital; Australia
Materia
CD4+ CELLS
COMBINATION ANTIRETROVIRAL THERAPY
GLUCOSE
GLUCOSE TRANSPORTER-1
HIV
IMMUNE ACTIVATION
INFLAMMATION
LYMPHOCYTES
METABOLISM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/85835
Acceder
id CONICETDig_493475fc489b47aed1d01c23572ef521
oai_identifier_str oai:ri.conicet.gov.ar:11336/85835
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Increased glucose metabolic activity is associated with CD4+ T-cell activation and depletion during chronic HIV infectionPalmer, Clovis S.Ostrowski, MatiasGouillou, MaelennTsai, LouisYu, DiZhou, JinglingHenstridge, Darren C.Maisa, AnnaHearps, Anna C.Lewin, Sharon R.Landay, AlanJaworowski, AnthonyMcCune, Joseph M.Crowe, Suzanne M.CD4+ CELLSCOMBINATION ANTIRETROVIRAL THERAPYGLUCOSEGLUCOSE TRANSPORTER-1HIVIMMUNE ACTIVATIONINFLAMMATIONLYMPHOCYTESMETABOLISMhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Objectives: Glucose metabolism plays a fundamental role in supporting the growth, proliferation and effector functions of T cells. We investigated the impact of HIV infection on key processes that regulate glucose uptake and metabolism in primary CD4+ and CD8+ T cells. Design and methods: Thirty-eight HIV-infected treatment-naive, 35 HIV+/ combination antiretroviral therapy, seven HIV+ long-term nonprogressors and 25 HIV control individuals were studied. Basal markers of glycolysis [e.g. glucose transporter-1 (Glut1) expression, glucose uptake, intracellular glucose-6-phosphate, and L-lactate] were measured in T cells. The cellular markers of immune activation, CD38 and HLADR, were measured by flow cytometry. Results: The surface expression of the Glut1 is up-regulated in CD4+ T cells in HIVinfected patients compared with uninfected controls. The percentage of circulating CD4+Glut1+ T cells was significantly increased in HIV-infected patients and was not restored to normal levels following combination antiretroviral therapy. Basal markers of glycolysis were significantly higher in CD4+Glut1+ T cells compared to CD4+Glut1- T cells. The proportion of CD4+Glut1+ T cells correlated positively with the expression of the cellular activation marker, HLA-DR, on total CD4+ T cells, but inversely with the absolute CD4+ T-cell count irrespective of HIV treatment status. Conclusion: Our data suggest that Glut1 is a potentially novel and functional marker of CD4+ T-cell activation during HIV infection. In addition, Glut1 expression on CD4+ T cells may be exploited as a prognostic marker for CD4+ T-cell loss during HIV disease progression.Fil: Palmer, Clovis S.. Burnet Institute; AustraliaFil: Ostrowski, Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; ArgentinaFil: Gouillou, Maelenn. Burnet Institute; AustraliaFil: Tsai, Louis. Monash University; AustraliaFil: Yu, Di. Monash University; AustraliaFil: Zhou, Jingling. Burnet Institute; AustraliaFil: Henstridge, Darren C.. Baker IDI Heart and Diabetes Institute; AustraliaFil: Maisa, Anna. Burnet Institute; AustraliaFil: Hearps, Anna C.. Burnet Institute; Australia. Monash University; AustraliaFil: Lewin, Sharon R.. Burnet Institute; Australia. Monash University; Australia. The Alfred Hospital; AustraliaFil: Landay, Alan. Rush University Medical Center; Estados UnidosFil: Jaworowski, Anthony. Burnet Institute; Australia. Monash University; AustraliaFil: McCune, Joseph M.. University of California; Estados UnidosFil: Crowe, Suzanne M.. Burnet Institute; Australia. Monash University; Australia. The Alfred Hospital; AustraliaLippincott Williams2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/85835Palmer, Clovis S.; Ostrowski, Matias; Gouillou, Maelenn; Tsai, Louis; Yu, Di; et al.; Increased glucose metabolic activity is associated with CD4+ T-cell activation and depletion during chronic HIV infection; Lippincott Williams; Aids; 28; 3; 1-2014; 297-3090269-9370CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1097/QAD.0000000000000128info:eu-repo/semantics/altIdentifier/url/https://insights.ovid.com/article/00002030-201401280-00002info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:49:57Zoai:ri.conicet.gov.ar:11336/85835instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:49:57.344CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Increased glucose metabolic activity is associated with CD4+ T-cell activation and depletion during chronic HIV infection
title Increased glucose metabolic activity is associated with CD4+ T-cell activation and depletion during chronic HIV infection
spellingShingle Increased glucose metabolic activity is associated with CD4+ T-cell activation and depletion during chronic HIV infection
Palmer, Clovis S.
CD4+ CELLS
COMBINATION ANTIRETROVIRAL THERAPY
GLUCOSE
GLUCOSE TRANSPORTER-1
HIV
IMMUNE ACTIVATION
INFLAMMATION
LYMPHOCYTES
METABOLISM
title_short Increased glucose metabolic activity is associated with CD4+ T-cell activation and depletion during chronic HIV infection
title_full Increased glucose metabolic activity is associated with CD4+ T-cell activation and depletion during chronic HIV infection
title_fullStr Increased glucose metabolic activity is associated with CD4+ T-cell activation and depletion during chronic HIV infection
title_full_unstemmed Increased glucose metabolic activity is associated with CD4+ T-cell activation and depletion during chronic HIV infection
title_sort Increased glucose metabolic activity is associated with CD4+ T-cell activation and depletion during chronic HIV infection
dc.creator.none.fl_str_mv Palmer, Clovis S.
Ostrowski, Matias
Gouillou, Maelenn
Tsai, Louis
Yu, Di
Zhou, Jingling
Henstridge, Darren C.
Maisa, Anna
Hearps, Anna C.
Lewin, Sharon R.
Landay, Alan
Jaworowski, Anthony
McCune, Joseph M.
Crowe, Suzanne M.
author Palmer, Clovis S.
author_facet Palmer, Clovis S.
Ostrowski, Matias
Gouillou, Maelenn
Tsai, Louis
Yu, Di
Zhou, Jingling
Henstridge, Darren C.
Maisa, Anna
Hearps, Anna C.
Lewin, Sharon R.
Landay, Alan
Jaworowski, Anthony
McCune, Joseph M.
Crowe, Suzanne M.
author_role author
author2 Ostrowski, Matias
Gouillou, Maelenn
Tsai, Louis
Yu, Di
Zhou, Jingling
Henstridge, Darren C.
Maisa, Anna
Hearps, Anna C.
Lewin, Sharon R.
Landay, Alan
Jaworowski, Anthony
McCune, Joseph M.
Crowe, Suzanne M.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CD4+ CELLS
COMBINATION ANTIRETROVIRAL THERAPY
GLUCOSE
GLUCOSE TRANSPORTER-1
HIV
IMMUNE ACTIVATION
INFLAMMATION
LYMPHOCYTES
METABOLISM
topic CD4+ CELLS
COMBINATION ANTIRETROVIRAL THERAPY
GLUCOSE
GLUCOSE TRANSPORTER-1
HIV
IMMUNE ACTIVATION
INFLAMMATION
LYMPHOCYTES
METABOLISM
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Objectives: Glucose metabolism plays a fundamental role in supporting the growth, proliferation and effector functions of T cells. We investigated the impact of HIV infection on key processes that regulate glucose uptake and metabolism in primary CD4+ and CD8+ T cells. Design and methods: Thirty-eight HIV-infected treatment-naive, 35 HIV+/ combination antiretroviral therapy, seven HIV+ long-term nonprogressors and 25 HIV control individuals were studied. Basal markers of glycolysis [e.g. glucose transporter-1 (Glut1) expression, glucose uptake, intracellular glucose-6-phosphate, and L-lactate] were measured in T cells. The cellular markers of immune activation, CD38 and HLADR, were measured by flow cytometry. Results: The surface expression of the Glut1 is up-regulated in CD4+ T cells in HIVinfected patients compared with uninfected controls. The percentage of circulating CD4+Glut1+ T cells was significantly increased in HIV-infected patients and was not restored to normal levels following combination antiretroviral therapy. Basal markers of glycolysis were significantly higher in CD4+Glut1+ T cells compared to CD4+Glut1- T cells. The proportion of CD4+Glut1+ T cells correlated positively with the expression of the cellular activation marker, HLA-DR, on total CD4+ T cells, but inversely with the absolute CD4+ T-cell count irrespective of HIV treatment status. Conclusion: Our data suggest that Glut1 is a potentially novel and functional marker of CD4+ T-cell activation during HIV infection. In addition, Glut1 expression on CD4+ T cells may be exploited as a prognostic marker for CD4+ T-cell loss during HIV disease progression.
Fil: Palmer, Clovis S.. Burnet Institute; Australia
Fil: Ostrowski, Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
Fil: Gouillou, Maelenn. Burnet Institute; Australia
Fil: Tsai, Louis. Monash University; Australia
Fil: Yu, Di. Monash University; Australia
Fil: Zhou, Jingling. Burnet Institute; Australia
Fil: Henstridge, Darren C.. Baker IDI Heart and Diabetes Institute; Australia
Fil: Maisa, Anna. Burnet Institute; Australia
Fil: Hearps, Anna C.. Burnet Institute; Australia. Monash University; Australia
Fil: Lewin, Sharon R.. Burnet Institute; Australia. Monash University; Australia. The Alfred Hospital; Australia
Fil: Landay, Alan. Rush University Medical Center; Estados Unidos
Fil: Jaworowski, Anthony. Burnet Institute; Australia. Monash University; Australia
Fil: McCune, Joseph M.. University of California; Estados Unidos
Fil: Crowe, Suzanne M.. Burnet Institute; Australia. Monash University; Australia. The Alfred Hospital; Australia
description Objectives: Glucose metabolism plays a fundamental role in supporting the growth, proliferation and effector functions of T cells. We investigated the impact of HIV infection on key processes that regulate glucose uptake and metabolism in primary CD4+ and CD8+ T cells. Design and methods: Thirty-eight HIV-infected treatment-naive, 35 HIV+/ combination antiretroviral therapy, seven HIV+ long-term nonprogressors and 25 HIV control individuals were studied. Basal markers of glycolysis [e.g. glucose transporter-1 (Glut1) expression, glucose uptake, intracellular glucose-6-phosphate, and L-lactate] were measured in T cells. The cellular markers of immune activation, CD38 and HLADR, were measured by flow cytometry. Results: The surface expression of the Glut1 is up-regulated in CD4+ T cells in HIVinfected patients compared with uninfected controls. The percentage of circulating CD4+Glut1+ T cells was significantly increased in HIV-infected patients and was not restored to normal levels following combination antiretroviral therapy. Basal markers of glycolysis were significantly higher in CD4+Glut1+ T cells compared to CD4+Glut1- T cells. The proportion of CD4+Glut1+ T cells correlated positively with the expression of the cellular activation marker, HLA-DR, on total CD4+ T cells, but inversely with the absolute CD4+ T-cell count irrespective of HIV treatment status. Conclusion: Our data suggest that Glut1 is a potentially novel and functional marker of CD4+ T-cell activation during HIV infection. In addition, Glut1 expression on CD4+ T cells may be exploited as a prognostic marker for CD4+ T-cell loss during HIV disease progression.
publishDate 2014
dc.date.none.fl_str_mv 2014-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/85835
Palmer, Clovis S.; Ostrowski, Matias; Gouillou, Maelenn; Tsai, Louis; Yu, Di; et al.; Increased glucose metabolic activity is associated with CD4+ T-cell activation and depletion during chronic HIV infection; Lippincott Williams; Aids; 28; 3; 1-2014; 297-309
0269-9370
CONICET Digital
CONICET
url http://hdl.handle.net/11336/85835
identifier_str_mv Palmer, Clovis S.; Ostrowski, Matias; Gouillou, Maelenn; Tsai, Louis; Yu, Di; et al.; Increased glucose metabolic activity is associated with CD4+ T-cell activation and depletion during chronic HIV infection; Lippincott Williams; Aids; 28; 3; 1-2014; 297-309
0269-9370
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1097/QAD.0000000000000128
info:eu-repo/semantics/altIdentifier/url/https://insights.ovid.com/article/00002030-201401280-00002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Lippincott Williams
publisher.none.fl_str_mv Lippincott Williams
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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