Confocal Raman spectroscopy: In vivo biochemical changes in the human skin by topical formulations under UV radiation

Autores
Gaspar Tosato, Maira; Orallo, Dalila Elisabet; Ali, S. M.; Churio, Maria Sandra; Martin, A. A.; Dicelio, Lelia Elina
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A new approach to the study of the effects on human skin of mycosporine-like amino acids (MAAs) and gadusol (Gad) incorporated in polymer gel is proposed in this work. The depth profile and photoprotector effects of Pluronic F127® gels containing each of the natural actives were evaluated by in vivo confocal Raman spectroscopy aiming at the analysis of the biochemical changes on human skin. Hierarchical cluster analysis (HCA) showed that the data corresponding to different depths of the skin, from surface to 4 μm, and from 6 to 16 μm, remained in the same cluster. In vivo Raman spectra, classified into five different layers of epidermis according to their similarities, indicated that the amount of Gad gel increased by about 26% in the outermost layer of the stratum corneum (SC) and that MAAs gel at 2 μm depth was 103.4% higher than in the outermost layer of the SC. Variations in the SC of urocanic acid at 1490-1515 cm- 1 and 1652 cm- 1 and histidine at 1318 cm- 1 were calculated, before and after UV exposure with or without gels. With the application of gels the vibrational modes that correspond to lipids in trans conformation (1063 and 1128 cm- 1) increased with respect to normal skin, whereas gauche conformation (1085 cm- 1) disappeared. Our studies suggest that gels protected the skin against the stress of the natural defense mechanism caused by high levels of UV exposure.
Fil: Gaspar Tosato, Maira. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Orallo, Dalila Elisabet. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ali, S. M.. Universidade do Vale do Paraíba; Brasil
Fil: Churio, Maria Sandra. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martin, A. A.. Universidade do Vale do Paraíba; Brasil
Fil: Dicelio, Lelia Elina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Materia
Confocal Raman
Gadusol
Human Skin
Mycosporine-Like Amino Acids
Urocanic Acid
Uv Radiation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/50184
Acceder
id CONICETDig_49146394d2370234e9e9e3b52d722193
oai_identifier_str oai:ri.conicet.gov.ar:11336/50184
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Confocal Raman spectroscopy: In vivo biochemical changes in the human skin by topical formulations under UV radiationGaspar Tosato, MairaOrallo, Dalila ElisabetAli, S. M.Churio, Maria SandraMartin, A. A.Dicelio, Lelia ElinaConfocal RamanGadusolHuman SkinMycosporine-Like Amino AcidsUrocanic AcidUv Radiationhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A new approach to the study of the effects on human skin of mycosporine-like amino acids (MAAs) and gadusol (Gad) incorporated in polymer gel is proposed in this work. The depth profile and photoprotector effects of Pluronic F127® gels containing each of the natural actives were evaluated by in vivo confocal Raman spectroscopy aiming at the analysis of the biochemical changes on human skin. Hierarchical cluster analysis (HCA) showed that the data corresponding to different depths of the skin, from surface to 4 μm, and from 6 to 16 μm, remained in the same cluster. In vivo Raman spectra, classified into five different layers of epidermis according to their similarities, indicated that the amount of Gad gel increased by about 26% in the outermost layer of the stratum corneum (SC) and that MAAs gel at 2 μm depth was 103.4% higher than in the outermost layer of the SC. Variations in the SC of urocanic acid at 1490-1515 cm- 1 and 1652 cm- 1 and histidine at 1318 cm- 1 were calculated, before and after UV exposure with or without gels. With the application of gels the vibrational modes that correspond to lipids in trans conformation (1063 and 1128 cm- 1) increased with respect to normal skin, whereas gauche conformation (1085 cm- 1) disappeared. Our studies suggest that gels protected the skin against the stress of the natural defense mechanism caused by high levels of UV exposure.Fil: Gaspar Tosato, Maira. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Orallo, Dalila Elisabet. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ali, S. M.. Universidade do Vale do Paraíba; BrasilFil: Churio, Maria Sandra. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Martin, A. A.. Universidade do Vale do Paraíba; BrasilFil: Dicelio, Lelia Elina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaElsevier Science Sa2015-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/50184Gaspar Tosato, Maira; Orallo, Dalila Elisabet; Ali, S. M.; Churio, Maria Sandra; Martin, A. A.; et al.; Confocal Raman spectroscopy: In vivo biochemical changes in the human skin by topical formulations under UV radiation; Elsevier Science Sa; Journal of Photochemistry and Photobiology B: Biology; 153; 12-2015; 51-581011-1344CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jphotobiol.2015.08.030info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1011134415002808info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:28:26Zoai:ri.conicet.gov.ar:11336/50184instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:28:26.318CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Confocal Raman spectroscopy: In vivo biochemical changes in the human skin by topical formulations under UV radiation
title Confocal Raman spectroscopy: In vivo biochemical changes in the human skin by topical formulations under UV radiation
spellingShingle Confocal Raman spectroscopy: In vivo biochemical changes in the human skin by topical formulations under UV radiation
Gaspar Tosato, Maira
Confocal Raman
Gadusol
Human Skin
Mycosporine-Like Amino Acids
Urocanic Acid
Uv Radiation
title_short Confocal Raman spectroscopy: In vivo biochemical changes in the human skin by topical formulations under UV radiation
title_full Confocal Raman spectroscopy: In vivo biochemical changes in the human skin by topical formulations under UV radiation
title_fullStr Confocal Raman spectroscopy: In vivo biochemical changes in the human skin by topical formulations under UV radiation
title_full_unstemmed Confocal Raman spectroscopy: In vivo biochemical changes in the human skin by topical formulations under UV radiation
title_sort Confocal Raman spectroscopy: In vivo biochemical changes in the human skin by topical formulations under UV radiation
dc.creator.none.fl_str_mv Gaspar Tosato, Maira
Orallo, Dalila Elisabet
Ali, S. M.
Churio, Maria Sandra
Martin, A. A.
Dicelio, Lelia Elina
author Gaspar Tosato, Maira
author_facet Gaspar Tosato, Maira
Orallo, Dalila Elisabet
Ali, S. M.
Churio, Maria Sandra
Martin, A. A.
Dicelio, Lelia Elina
author_role author
author2 Orallo, Dalila Elisabet
Ali, S. M.
Churio, Maria Sandra
Martin, A. A.
Dicelio, Lelia Elina
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Confocal Raman
Gadusol
Human Skin
Mycosporine-Like Amino Acids
Urocanic Acid
Uv Radiation
topic Confocal Raman
Gadusol
Human Skin
Mycosporine-Like Amino Acids
Urocanic Acid
Uv Radiation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A new approach to the study of the effects on human skin of mycosporine-like amino acids (MAAs) and gadusol (Gad) incorporated in polymer gel is proposed in this work. The depth profile and photoprotector effects of Pluronic F127® gels containing each of the natural actives were evaluated by in vivo confocal Raman spectroscopy aiming at the analysis of the biochemical changes on human skin. Hierarchical cluster analysis (HCA) showed that the data corresponding to different depths of the skin, from surface to 4 μm, and from 6 to 16 μm, remained in the same cluster. In vivo Raman spectra, classified into five different layers of epidermis according to their similarities, indicated that the amount of Gad gel increased by about 26% in the outermost layer of the stratum corneum (SC) and that MAAs gel at 2 μm depth was 103.4% higher than in the outermost layer of the SC. Variations in the SC of urocanic acid at 1490-1515 cm- 1 and 1652 cm- 1 and histidine at 1318 cm- 1 were calculated, before and after UV exposure with or without gels. With the application of gels the vibrational modes that correspond to lipids in trans conformation (1063 and 1128 cm- 1) increased with respect to normal skin, whereas gauche conformation (1085 cm- 1) disappeared. Our studies suggest that gels protected the skin against the stress of the natural defense mechanism caused by high levels of UV exposure.
Fil: Gaspar Tosato, Maira. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Orallo, Dalila Elisabet. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ali, S. M.. Universidade do Vale do Paraíba; Brasil
Fil: Churio, Maria Sandra. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martin, A. A.. Universidade do Vale do Paraíba; Brasil
Fil: Dicelio, Lelia Elina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
description A new approach to the study of the effects on human skin of mycosporine-like amino acids (MAAs) and gadusol (Gad) incorporated in polymer gel is proposed in this work. The depth profile and photoprotector effects of Pluronic F127® gels containing each of the natural actives were evaluated by in vivo confocal Raman spectroscopy aiming at the analysis of the biochemical changes on human skin. Hierarchical cluster analysis (HCA) showed that the data corresponding to different depths of the skin, from surface to 4 μm, and from 6 to 16 μm, remained in the same cluster. In vivo Raman spectra, classified into five different layers of epidermis according to their similarities, indicated that the amount of Gad gel increased by about 26% in the outermost layer of the stratum corneum (SC) and that MAAs gel at 2 μm depth was 103.4% higher than in the outermost layer of the SC. Variations in the SC of urocanic acid at 1490-1515 cm- 1 and 1652 cm- 1 and histidine at 1318 cm- 1 were calculated, before and after UV exposure with or without gels. With the application of gels the vibrational modes that correspond to lipids in trans conformation (1063 and 1128 cm- 1) increased with respect to normal skin, whereas gauche conformation (1085 cm- 1) disappeared. Our studies suggest that gels protected the skin against the stress of the natural defense mechanism caused by high levels of UV exposure.
publishDate 2015
dc.date.none.fl_str_mv 2015-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/50184
Gaspar Tosato, Maira; Orallo, Dalila Elisabet; Ali, S. M.; Churio, Maria Sandra; Martin, A. A.; et al.; Confocal Raman spectroscopy: In vivo biochemical changes in the human skin by topical formulations under UV radiation; Elsevier Science Sa; Journal of Photochemistry and Photobiology B: Biology; 153; 12-2015; 51-58
1011-1344
CONICET Digital
CONICET
url http://hdl.handle.net/11336/50184
identifier_str_mv Gaspar Tosato, Maira; Orallo, Dalila Elisabet; Ali, S. M.; Churio, Maria Sandra; Martin, A. A.; et al.; Confocal Raman spectroscopy: In vivo biochemical changes in the human skin by topical formulations under UV radiation; Elsevier Science Sa; Journal of Photochemistry and Photobiology B: Biology; 153; 12-2015; 51-58
1011-1344
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jphotobiol.2015.08.030
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1011134415002808
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Sa
publisher.none.fl_str_mv Elsevier Science Sa
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781862038470656
score 12.982451

Publicaciones similares