Confocal Raman spectroscopy: determination of natural moisturizing factor profile related to skin hydration

Autores
Teixeira, Ana Paula; Rangel, João Lucas; Raniero, Leandro José; Gaspar Tosato, Maira; Pereira Fávero, Priscila; Martin, Airton Abrahao
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Skin health and skin care to reduce the effects of aging are the main interests of many researchers. The skin is very important because it protects the body from various effects of the external environment, and studies of the largest organ of the human body have been conducted since antiquity. In skin, aging effects are severe enough to promote changes in cell structure and biochemical composition. In this study, we quantitatively analyzed the water content and natural moisturizing factor of human facial skin in vivo and in real time by confocal Raman spectroscopy. This non-invasive technique is capable of providing detailed information on the biochemical composition at different depth profiles in the skin. Methods: We studied 10 volunteers, phototype II (40 and 50 years old), using a confocal Raman system to examine the skin surface down to 25 μm. Raman spectra were obtained before product use (T0), and after 30 days of continuous use of cosmetics (T30). Results: The results show a significant increase of 6.4% in water content in the surface layer of the facial skin after the cosmetic use. The amounts of natural moisturizing factor (NMF) compounds were also increased. Urocanic acid underwent a greater change in relation to carboxylic acid pyrrolidone, with a 38.5% increase in the stratum corneum. Conclusion: Confocal Raman spectroscopy identified changes in the biochemical composition of the superficial layers of the epidermis, which suggests the anti-aging efficacy of the formulation.
Fil: Teixeira, Ana Paula . Universidade do Vale do Paraiba. Instituto de Pesquisa e Desenvolvimento; Brasil
Fil: Rangel, João Lucas . Universidade do Vale do Paraiba. Instituto de Pesquisa e Desenvolvimento; Brasil
Fil: Raniero, Leandro José . Universidade do Vale do Paraiba. Instituto de Pesquisa e Desenvolvimento; Brasil
Fil: Gaspar Tosato, Maira. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires; Argentina
Fil: Pereira Fávero, Priscila . Universidade do Vale do Paraiba. Instituto de Pesquisa e Desenvolvimento; Brasil
Fil: Martin, Airton Abrahao. Universidade do Vale do Paraiba. Instituto de Pesquisa e Desenvolvimento; Brasil
Materia
SKIN AGING
COSMETICS
CONFOCAL RAMAN SPECTROSCOPY
NMF
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/9204
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/9204
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Confocal Raman spectroscopy: determination of natural moisturizing factor profile related to skin hydrationTeixeira, Ana Paula Rangel, João Lucas Raniero, Leandro José Gaspar Tosato, MairaPereira Fávero, Priscila Martin, Airton AbrahaoSKIN AGINGCOSMETICSCONFOCAL RAMAN SPECTROSCOPYNMFhttps://purl.org/becyt/ford/2.6https://purl.org/becyt/ford/2Skin health and skin care to reduce the effects of aging are the main interests of many researchers. The skin is very important because it protects the body from various effects of the external environment, and studies of the largest organ of the human body have been conducted since antiquity. In skin, aging effects are severe enough to promote changes in cell structure and biochemical composition. In this study, we quantitatively analyzed the water content and natural moisturizing factor of human facial skin in vivo and in real time by confocal Raman spectroscopy. This non-invasive technique is capable of providing detailed information on the biochemical composition at different depth profiles in the skin. Methods: We studied 10 volunteers, phototype II (40 and 50 years old), using a confocal Raman system to examine the skin surface down to 25 μm. Raman spectra were obtained before product use (T0), and after 30 days of continuous use of cosmetics (T30). Results: The results show a significant increase of 6.4% in water content in the surface layer of the facial skin after the cosmetic use. The amounts of natural moisturizing factor (NMF) compounds were also increased. Urocanic acid underwent a greater change in relation to carboxylic acid pyrrolidone, with a 38.5% increase in the stratum corneum. Conclusion: Confocal Raman spectroscopy identified changes in the biochemical composition of the superficial layers of the epidermis, which suggests the anti-aging efficacy of the formulation.Fil: Teixeira, Ana Paula . Universidade do Vale do Paraiba. Instituto de Pesquisa e Desenvolvimento; BrasilFil: Rangel, João Lucas . Universidade do Vale do Paraiba. Instituto de Pesquisa e Desenvolvimento; BrasilFil: Raniero, Leandro José . Universidade do Vale do Paraiba. Instituto de Pesquisa e Desenvolvimento; BrasilFil: Gaspar Tosato, Maira. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires; ArgentinaFil: Pereira Fávero, Priscila . Universidade do Vale do Paraiba. Instituto de Pesquisa e Desenvolvimento; BrasilFil: Martin, Airton Abrahao. Universidade do Vale do Paraiba. Instituto de Pesquisa e Desenvolvimento; Brasil2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/9204Teixeira, Ana Paula ; Rangel, João Lucas ; Raniero, Leandro José ; Gaspar Tosato, Maira; Pereira Fávero, Priscila ; et al.; Confocal Raman spectroscopy: determination of natural moisturizing factor profile related to skin hydration; Revista Brasileira de Engenharia Biomedica; 30; 1; 3-2014; 1-61517-3151enginfo:eu-repo/semantics/altIdentifier/doi/10.4322/rbeb.2014.002info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/8x9nqhinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:44:01Zoai:ri.conicet.gov.ar:11336/9204instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:44:01.357CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Confocal Raman spectroscopy: determination of natural moisturizing factor profile related to skin hydration
title Confocal Raman spectroscopy: determination of natural moisturizing factor profile related to skin hydration
spellingShingle Confocal Raman spectroscopy: determination of natural moisturizing factor profile related to skin hydration
Teixeira, Ana Paula
SKIN AGING
COSMETICS
CONFOCAL RAMAN SPECTROSCOPY
NMF
title_short Confocal Raman spectroscopy: determination of natural moisturizing factor profile related to skin hydration
title_full Confocal Raman spectroscopy: determination of natural moisturizing factor profile related to skin hydration
title_fullStr Confocal Raman spectroscopy: determination of natural moisturizing factor profile related to skin hydration
title_full_unstemmed Confocal Raman spectroscopy: determination of natural moisturizing factor profile related to skin hydration
title_sort Confocal Raman spectroscopy: determination of natural moisturizing factor profile related to skin hydration
dc.creator.none.fl_str_mv Teixeira, Ana Paula
Rangel, João Lucas
Raniero, Leandro José
Gaspar Tosato, Maira
Pereira Fávero, Priscila
Martin, Airton Abrahao
author Teixeira, Ana Paula
author_facet Teixeira, Ana Paula
Rangel, João Lucas
Raniero, Leandro José
Gaspar Tosato, Maira
Pereira Fávero, Priscila
Martin, Airton Abrahao
author_role author
author2 Rangel, João Lucas
Raniero, Leandro José
Gaspar Tosato, Maira
Pereira Fávero, Priscila
Martin, Airton Abrahao
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv SKIN AGING
COSMETICS
CONFOCAL RAMAN SPECTROSCOPY
NMF
topic SKIN AGING
COSMETICS
CONFOCAL RAMAN SPECTROSCOPY
NMF
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.6
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Skin health and skin care to reduce the effects of aging are the main interests of many researchers. The skin is very important because it protects the body from various effects of the external environment, and studies of the largest organ of the human body have been conducted since antiquity. In skin, aging effects are severe enough to promote changes in cell structure and biochemical composition. In this study, we quantitatively analyzed the water content and natural moisturizing factor of human facial skin in vivo and in real time by confocal Raman spectroscopy. This non-invasive technique is capable of providing detailed information on the biochemical composition at different depth profiles in the skin. Methods: We studied 10 volunteers, phototype II (40 and 50 years old), using a confocal Raman system to examine the skin surface down to 25 μm. Raman spectra were obtained before product use (T0), and after 30 days of continuous use of cosmetics (T30). Results: The results show a significant increase of 6.4% in water content in the surface layer of the facial skin after the cosmetic use. The amounts of natural moisturizing factor (NMF) compounds were also increased. Urocanic acid underwent a greater change in relation to carboxylic acid pyrrolidone, with a 38.5% increase in the stratum corneum. Conclusion: Confocal Raman spectroscopy identified changes in the biochemical composition of the superficial layers of the epidermis, which suggests the anti-aging efficacy of the formulation.
Fil: Teixeira, Ana Paula . Universidade do Vale do Paraiba. Instituto de Pesquisa e Desenvolvimento; Brasil
Fil: Rangel, João Lucas . Universidade do Vale do Paraiba. Instituto de Pesquisa e Desenvolvimento; Brasil
Fil: Raniero, Leandro José . Universidade do Vale do Paraiba. Instituto de Pesquisa e Desenvolvimento; Brasil
Fil: Gaspar Tosato, Maira. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires; Argentina
Fil: Pereira Fávero, Priscila . Universidade do Vale do Paraiba. Instituto de Pesquisa e Desenvolvimento; Brasil
Fil: Martin, Airton Abrahao. Universidade do Vale do Paraiba. Instituto de Pesquisa e Desenvolvimento; Brasil
description Skin health and skin care to reduce the effects of aging are the main interests of many researchers. The skin is very important because it protects the body from various effects of the external environment, and studies of the largest organ of the human body have been conducted since antiquity. In skin, aging effects are severe enough to promote changes in cell structure and biochemical composition. In this study, we quantitatively analyzed the water content and natural moisturizing factor of human facial skin in vivo and in real time by confocal Raman spectroscopy. This non-invasive technique is capable of providing detailed information on the biochemical composition at different depth profiles in the skin. Methods: We studied 10 volunteers, phototype II (40 and 50 years old), using a confocal Raman system to examine the skin surface down to 25 μm. Raman spectra were obtained before product use (T0), and after 30 days of continuous use of cosmetics (T30). Results: The results show a significant increase of 6.4% in water content in the surface layer of the facial skin after the cosmetic use. The amounts of natural moisturizing factor (NMF) compounds were also increased. Urocanic acid underwent a greater change in relation to carboxylic acid pyrrolidone, with a 38.5% increase in the stratum corneum. Conclusion: Confocal Raman spectroscopy identified changes in the biochemical composition of the superficial layers of the epidermis, which suggests the anti-aging efficacy of the formulation.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/9204
Teixeira, Ana Paula ; Rangel, João Lucas ; Raniero, Leandro José ; Gaspar Tosato, Maira; Pereira Fávero, Priscila ; et al.; Confocal Raman spectroscopy: determination of natural moisturizing factor profile related to skin hydration; Revista Brasileira de Engenharia Biomedica; 30; 1; 3-2014; 1-6
1517-3151
url http://hdl.handle.net/11336/9204
identifier_str_mv Teixeira, Ana Paula ; Rangel, João Lucas ; Raniero, Leandro José ; Gaspar Tosato, Maira; Pereira Fávero, Priscila ; et al.; Confocal Raman spectroscopy: determination of natural moisturizing factor profile related to skin hydration; Revista Brasileira de Engenharia Biomedica; 30; 1; 3-2014; 1-6
1517-3151
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4322/rbeb.2014.002
info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/8x9nqh
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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