Estudio de los genes alinasa y quitinasa en materiales de ajo costarricense (Allium sativum L.).

Autores
Barboza Rojas, Karina; Hernández Soto, Alejandro; Zúñiga Vega, Claudia
Año de publicación
2013
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
AbstractGarlic production in Costa Rica has been affected by the quality and quantity of the harvested seeds. Bulb production has also been deteriorated by diseases.However, this crop is preferred for its flavor, considered superior to the one imported from China. Pungency of garlic is partially due to the action of the alliinase enzyme. Furthermore, the resistance to certain pathogenic fungi is influenced by the chitinase enzyme activity. The encoding genes for both enzymes were analyzed in this study, by using in vitro plantlets obtained from local materials from Llano Grande, Santa Ana, Miramar and San Ramon zones and garlic imported from China. By using these genes, DNA sequences were compared and studied in order to find differences that allow the characterization of various materials. The results showed the presence of different alliinase gene copies. The chitinase gene presented a highly conserved sequence in all materials tested. Two highly conserved introns were found in the Costa Rican germplasm and the Asian reference material. It was concluded that Costa Rican garlic is very similar to the Asian. Also, the first report of the existence of introns in thechitinase of garlic is presented here.
Fil: Barboza Rojas, Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Cuyo Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Hernández Soto, Alejandro. Instituto Tecnologico de Costa Rica; Costa Rica
Fil: Zúñiga Vega, Claudia. Instituto Tecnologico de Costa Rica; Costa Rica
Materia
ALLINASA
QUITINASA
AJO COSTARRICENSE
ALLIUM SATIVUM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7662
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Estudio de los genes alinasa y quitinasa en materiales de ajo costarricense (Allium sativum L.).Study of the genes alliinase and chitinase in materials of costarican garlic (Allium sativum L)Barboza Rojas, KarinaHernández Soto, AlejandroZúñiga Vega, ClaudiaALLINASAQUITINASAAJO COSTARRICENSEALLIUM SATIVUMhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1AbstractGarlic production in Costa Rica has been affected by the quality and quantity of the harvested seeds. Bulb production has also been deteriorated by diseases.However, this crop is preferred for its flavor, considered superior to the one imported from China. Pungency of garlic is partially due to the action of the alliinase enzyme. Furthermore, the resistance to certain pathogenic fungi is influenced by the chitinase enzyme activity. The encoding genes for both enzymes were analyzed in this study, by using in vitro plantlets obtained from local materials from Llano Grande, Santa Ana, Miramar and San Ramon zones and garlic imported from China. By using these genes, DNA sequences were compared and studied in order to find differences that allow the characterization of various materials. The results showed the presence of different alliinase gene copies. The chitinase gene presented a highly conserved sequence in all materials tested. Two highly conserved introns were found in the Costa Rican germplasm and the Asian reference material. It was concluded that Costa Rican garlic is very similar to the Asian. Also, the first report of the existence of introns in thechitinase of garlic is presented here.Fil: Barboza Rojas, Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Cuyo Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; ArgentinaFil: Hernández Soto, Alejandro. Instituto Tecnologico de Costa Rica; Costa RicaFil: Zúñiga Vega, Claudia. Instituto Tecnologico de Costa Rica; Costa RicaEditorial Tecnológica de Costa Rica2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7662Barboza Rojas, Karina; Hernández Soto, Alejandro; Zúñiga Vega, Claudia; Estudio de los genes alinasa y quitinasa en materiales de ajo costarricense (Allium sativum L.).; Editorial Tecnológica de Costa Rica; Tecnologia en Marcha; 26; 1; 1-2013; 46-530379-3982spainfo:eu-repo/semantics/altIdentifier/url/http://revistas.tec.ac.cr/index.php/tec_marcha/article/view/1121info:eu-repo/semantics/altIdentifier/doi/10.18845/tm.v26i1.1121info:eu-repo/semantics/altIdentifier/url/https://dialnet.unirioja.es/servlet/articulo?codigo=4835675info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:34:59Zoai:ri.conicet.gov.ar:11336/7662instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:35:00.16CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Estudio de los genes alinasa y quitinasa en materiales de ajo costarricense (Allium sativum L.).
Study of the genes alliinase and chitinase in materials of costarican garlic (Allium sativum L)
title Estudio de los genes alinasa y quitinasa en materiales de ajo costarricense (Allium sativum L.).
spellingShingle Estudio de los genes alinasa y quitinasa en materiales de ajo costarricense (Allium sativum L.).
Barboza Rojas, Karina
ALLINASA
QUITINASA
AJO COSTARRICENSE
ALLIUM SATIVUM
title_short Estudio de los genes alinasa y quitinasa en materiales de ajo costarricense (Allium sativum L.).
title_full Estudio de los genes alinasa y quitinasa en materiales de ajo costarricense (Allium sativum L.).
title_fullStr Estudio de los genes alinasa y quitinasa en materiales de ajo costarricense (Allium sativum L.).
title_full_unstemmed Estudio de los genes alinasa y quitinasa en materiales de ajo costarricense (Allium sativum L.).
title_sort Estudio de los genes alinasa y quitinasa en materiales de ajo costarricense (Allium sativum L.).
dc.creator.none.fl_str_mv Barboza Rojas, Karina
Hernández Soto, Alejandro
Zúñiga Vega, Claudia
author Barboza Rojas, Karina
author_facet Barboza Rojas, Karina
Hernández Soto, Alejandro
Zúñiga Vega, Claudia
author_role author
author2 Hernández Soto, Alejandro
Zúñiga Vega, Claudia
author2_role author
author
dc.subject.none.fl_str_mv ALLINASA
QUITINASA
AJO COSTARRICENSE
ALLIUM SATIVUM
topic ALLINASA
QUITINASA
AJO COSTARRICENSE
ALLIUM SATIVUM
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv AbstractGarlic production in Costa Rica has been affected by the quality and quantity of the harvested seeds. Bulb production has also been deteriorated by diseases.However, this crop is preferred for its flavor, considered superior to the one imported from China. Pungency of garlic is partially due to the action of the alliinase enzyme. Furthermore, the resistance to certain pathogenic fungi is influenced by the chitinase enzyme activity. The encoding genes for both enzymes were analyzed in this study, by using in vitro plantlets obtained from local materials from Llano Grande, Santa Ana, Miramar and San Ramon zones and garlic imported from China. By using these genes, DNA sequences were compared and studied in order to find differences that allow the characterization of various materials. The results showed the presence of different alliinase gene copies. The chitinase gene presented a highly conserved sequence in all materials tested. Two highly conserved introns were found in the Costa Rican germplasm and the Asian reference material. It was concluded that Costa Rican garlic is very similar to the Asian. Also, the first report of the existence of introns in thechitinase of garlic is presented here.
Fil: Barboza Rojas, Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Cuyo Mendoza-San Juan. Estación Experimental Agropecuaria La Consulta; Argentina
Fil: Hernández Soto, Alejandro. Instituto Tecnologico de Costa Rica; Costa Rica
Fil: Zúñiga Vega, Claudia. Instituto Tecnologico de Costa Rica; Costa Rica
description AbstractGarlic production in Costa Rica has been affected by the quality and quantity of the harvested seeds. Bulb production has also been deteriorated by diseases.However, this crop is preferred for its flavor, considered superior to the one imported from China. Pungency of garlic is partially due to the action of the alliinase enzyme. Furthermore, the resistance to certain pathogenic fungi is influenced by the chitinase enzyme activity. The encoding genes for both enzymes were analyzed in this study, by using in vitro plantlets obtained from local materials from Llano Grande, Santa Ana, Miramar and San Ramon zones and garlic imported from China. By using these genes, DNA sequences were compared and studied in order to find differences that allow the characterization of various materials. The results showed the presence of different alliinase gene copies. The chitinase gene presented a highly conserved sequence in all materials tested. Two highly conserved introns were found in the Costa Rican germplasm and the Asian reference material. It was concluded that Costa Rican garlic is very similar to the Asian. Also, the first report of the existence of introns in thechitinase of garlic is presented here.
publishDate 2013
dc.date.none.fl_str_mv 2013-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7662
Barboza Rojas, Karina; Hernández Soto, Alejandro; Zúñiga Vega, Claudia; Estudio de los genes alinasa y quitinasa en materiales de ajo costarricense (Allium sativum L.).; Editorial Tecnológica de Costa Rica; Tecnologia en Marcha; 26; 1; 1-2013; 46-53
0379-3982
url http://hdl.handle.net/11336/7662
identifier_str_mv Barboza Rojas, Karina; Hernández Soto, Alejandro; Zúñiga Vega, Claudia; Estudio de los genes alinasa y quitinasa en materiales de ajo costarricense (Allium sativum L.).; Editorial Tecnológica de Costa Rica; Tecnologia en Marcha; 26; 1; 1-2013; 46-53
0379-3982
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://revistas.tec.ac.cr/index.php/tec_marcha/article/view/1121
info:eu-repo/semantics/altIdentifier/doi/10.18845/tm.v26i1.1121
info:eu-repo/semantics/altIdentifier/url/https://dialnet.unirioja.es/servlet/articulo?codigo=4835675
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Editorial Tecnológica de Costa Rica
publisher.none.fl_str_mv Editorial Tecnológica de Costa Rica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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